• Journal of Life Science
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• v.17 no.3 s.83
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• pp.386-395
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• 2007

A total of 27 strains of Vibrio parahaemolyticus (18 strains isolated from Korea and 9 strains from Japan) were serotyped and examined for biochemical characteristics, antimicrobial susceptibility patterns, cytotoxicity assay, thermostable direct hemolysin (TDH) production and molecular epidemiology. Using polymerase chain reaction (PCR) method and DNA probe hybridization method, the strains were tested for toxR, tdh, trh and ORF 8 genes. The V. parahaemolyticus isolated from patients were belonged to 8 different serotypes : O3:K6, O1:K38, O3:K57, O4:K9, O4:Kl2, O4:K68, O5:Kl5 and O6:K46. Urease-positive strain possessed the trh gene, and conversely, urease-negative strains lacked the gene, indicating that urease production by V. parahemolyticus strains strongly correlates with the possession of the trh gene. Most strains showed multiple resistant to more than three antibiotics and the antibiogram could be classified into 6 group (I to VI). All of the O3:K6 strains isolated in South Korea and Japan producted TDH at high levels. The TDH titers ranged between 256 and 2.048, and the average titer was 1009. To distinguish the new and increasingly common V. parahaemolyticus strains from clinical isolates, ORF 8 is a useful genetic marker. After Southern hybridization, the HindIII restriction fragment patterns of the tdh gene were grouped one type, respectively. One type showed two bands one of which was 4.3kb and the other was 11.5kb in size. Variation between the O3:K6 serotype are minor when compared to the differences seen with the non O3:K6 strains. The migration patterns of Not I -digested of the total DNA of the O3:K6 strains were similar, and only slight variations were observed between the serotypes. By contrast, the O3:K6 strains and non O3:K6 had markedly different profiles. In conclusion, Random amplified polymorphic DNA (RAPD) profile using appropriate primers was an effective epidemiological marker.

• Journal of Mechanical Science and Technology
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• v.16 no.6
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• pp.851-860
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• 2002

In this study, it was attempted to obtain the fundamental data for the formation and oxidation of soot from a diesel engine. Combustion of spray injected into a cylinder is complex phenomenon having physical and chemical processes, and these processes affect each other. There are many factors in the mechanism of the formation and oxidization of soot and it is necessary to observe spray combustion microscopically. In order to observe with that view, free fuel droplet array was used as an experimental object and the droplet array was injected into an atmospheric combustion chamber with high temperature. Ambient temperature of the combustion chamber, interdroplet spacing, and droplet diameter were selected as parameters, which affect the formation and oxidation of soot. In this study, it was found that the parameters also affect ignition delay of droplet. The ambient temperature especially affected the ignition delay of droplet as well as the flame temperature after self-ignition. As the interdroplet spacing that means the local equivalence ratio in a combustion chamber was narrow, formation of soot was increased. As diameter of droplet was large, surface area of the droplet was also broad, and hence evaporation of the droplet was more active than that of a droplet with relative small diameter.

• Journal of Advanced Marine Engineering and Technology
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• v.29 no.6
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• pp.662-670
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• 2005

With highly oxygenated fuels the smoke emissions decreased sharply and linearly with increases in the fuel oxygen content and entirely disappeared at an oxygen content of $38wt-\%$ even at stoichiometric mixture conditions The NOx also decreased monotonically with increases in oxygen content. and thermal efficiency slightly improved because of a reduction in cooling loss and improvement in the degree of constant volume combustion. The mechanisms of the significant reductions in emissions and improvement in engine performance were analyzed with a bottom view type DI diesel engine. Together with direct flame images, flame images were taken through an optical fetter passing only two wavelengths for use in 2-D two-color analysis. The results showed that luminous flame decreased significantly with increases in oxygen content and was not detected for neat dimethoxy methane(DMM). The decrease in flame luminosity with highly oxygenated fuels corresponds with decreases in soot and cooling losses, including those due to heat radiation. The 2-D two-color flame analysis indicated that the high temperature flame and high KL factor areas apparently decreased with increasing fuel oxygen content. These results correspond strongly with decreases in NOx. smoke. and cooling loss with increases in oxygen content.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.31 no.2 s.257
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• pp.132-140
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• 2007

The temperature and soot of the visualized diesel engine's turbulent flow of the flame was measured qualitatively. In the combustion chamber, in order to judge the affect that the swirl current has on the current ratio two heads with different ratios were used. Using a high speed camera, the results were analyzed using flame visualization. In order to measure the temperature and soot of the turbulent flames like diesel flames, two color methods were used to acquire temperature and the soot of the flames according to the conditions through analyzing the two wavelengths of the flames. It was possible to measure the highest temperature of the non-swirl head visualized engine, which is approximately 2400K, and that swirl head engine managed up to 2100K. With respect to the visualized diesel engine soot, we got the grasp of the KL factor which bears the qualitative information of the soot. This study is dedicated to suggesting the possibility of measuring not only the temperature but also soot of the diffusion flame of the diesel engine turbulent flames.

• Transactions of the Korean Society of Automotive Engineers
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• v.15 no.1
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• pp.177-185
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• 2007

The temperature and soot of the visualized diesel engine's turbulent flow of flame was qualitatively measured. In combustion chamber, in order to judge the affect that the swirl has on the in-cylinder's current, was used two different heads with different values. Using the high speed camera, and the results were analyzed using the heat release rate produced by the pressure sensor. In order to measure the temperature and soot of the turbulent flames like that of the diesel flames two color methods were used temperature and the soot of the flames according to the conditions through analyzing the two wavelengths of the flames. It was possible to measure the highest temperature of the non-swirl head visualized engine which is approximately 2400K, and that swirl head engine managed up to 2100K. With respect to the visualized diesel engine soot, we got the grasp of the KL factor which bears the qualitative information of soot. This study is dedicated to suggesting the possibility of measuring not only the temperature but also soot of the diffusion flame of the diesel engine turbulent flames through such method.

• Journal of Digital Convergence
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• v.10 no.6
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• pp.205-211
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• 2012

According to the Hebei Spirit incident, 12,547kl of crude oil was spilled into the sea and a total of approximately 40,000 households was damaged. Also, eleven cities and counties including "Taean" were designated as a Special Disaster District by government. Large oil pollution incidents cause severe impact on the change of the surrounding environment as well as destruction of the natural environment of damaged areas. This study analyzes the impacts on the surrounding environment of damaged areas caused by Hebei Spirit oil pollution incident, focusing on the largest damaged county called Taean. Survey was performed with items which are changes in population and local economy, effects on public health, social conflicts and etc. The result of analysis can be used as an important factor to forecast the impact on a damaged area in case of future oil pollution incidents and to establish effective policy of government.

• Journal of the Korean Institute of Gas
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• v.18 no.2
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• pp.69-72
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• 2014

If LNG is leaked from 9% Ni steel inner tank by damage, LNG is retained by outer concrete tank. Then large tensile stress can be caused at cylindrical bottom of outer tank by temperature difference between outer and inner surface of outer tank. Therefore, in order to reduce the tensile stress is caused by temperature difference, corner-protection is installed with insulation and 9% Ni steel as a second barrier. In this paper, using finite element method, structural analysis was performed for rectangular and circular shape of knuckle and based on the results, fatigue life of welds of corner protection was predicted. As a consequence of structural analysis, safety factor of circular knuckle shows 33% bigger than rectangular one shows, and circular knuckle has 25% bigger fatigue life time than rectangle has. These results can be applied to life time assessment and design optimization in the future.

• Journal of the Korean Society for Nondestructive Testing
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• v.22 no.5
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• pp.490-499
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• 2002

Recently, gamma radiographic equipments have been used about 1,000 sets manually and operated by about 2,500 persons in Korea. In order for a radiography to work effectively with avoiding any hazard of the high level radiation from the source, many field workers have expected developing a wireless automatic remote exposure controller. The KlTCO research team has developed an automatic remote exposure controller that can regulate the speed of $0.4{\sim}1.2m/s$ by BLDC motor of 24V 200W which has output of $54kgf{\cdot}cm$, suitable torque and safety factor for the work. And the developed automatic remote exposure controller can control rpm of motor, pigtail position by photo-sensor and exposure time by timer to RF sensor. Thus, the developed equipment is expected that the unit can be used in many practical applications with benefits in economical advantage to combine the use of both automatic and manual type because attachment is possible existent manual remote exposure controller, AC and DC combined use.