• Title/Summary/Keyword: KIT-1

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Studies on the Pregnancy Diagnosis by Easy Measurement of Serum (유우의 혈청내 Progesterone 농도의 간역측정에 의한 임신판단에 관한 연구)

  • 김상근;김민규;신현주;이만휘;이명훈
    • Korean Journal of Animal Reproduction
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    • v.13 no.3
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    • pp.157-163
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    • 1989
  • This stduy was carried out to evaluate the ability of clinical application of pregnancy diagnosis based upon the determination of progesterone in serum, utilizing EIA-kit of progesterone concentrations in the serum were assayed by radioimmunoassay. 1. The progesterone concentrations of the pregnant cows(2.40$\pm$0.34ng/ml) were significantly higher than those of non-pregnant cows(1.03$\pm$0.09ng/ml), and thereafter began to increase and maintained high levels. 2. During 20 to 22 days after artificial insemination, the accuracy of pregnancy diagnosis from EIA-kit of progesterone were 95.0% for non-pregnant cows, and 92.3% for pregnant cows. 3. During 20 to 22 days after artificial insemination, the accuracy of pregnancy diagnosis from serum progesterone concentrations were 100% for non-pregnant cows(<1.4ng/ml), and 96.2% for pregnant cows( 2.0ng/ml). The average overall accuracy of prediction for pregnant and non-pregnant cows were 98.1%. 4. Accordingly, the pregnancy diagnosis from EIA-kit of progesterone is thought to be recommendable because this early diagnostic means are simple with accurate results.

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A STUDY ON THE GLOSS AND ROUGHNESS OF THE COMPOSITE RESIN (복합레진의 광택 및 표면조도에 관한 연구)

  • Cho, Seung-Joo;Lee, Myung-Jong
    • Restorative Dentistry and Endodontics
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    • v.15 no.1
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    • pp.67-80
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    • 1990
  • This study was performed for elucidating the effects on surface polishing of composite resins. In this study, Silux(microfilled), Graft(hybrid), Bisfil- I (hybrid posterior) and Hi-pol(conventional) were used. Sixty specimens were made with 4 brands of composite resins and Optilux system in $2.0{\times}1.3{\times}1.0cm$ resin block which has a cavity with 0.5cm diameter and 0.5cm depth. Polishing was done with #600 sand paper and Soflex, Super-snap, Micron finishing system, or Composite polishing kit. Final polished surfaces were measured by roughness tester(Kasaka Lab. Ltd., Japan) and image analyser(Omnimet Image Analyser, Buehler, USA). The results were as follows, 1. The celluloid strip produced the smoothest surfaces. 2. Light curing microfilled composite resin, Silux, had smoother surface than any others. 3. The surfaces polished by Soflex were smoothest. 4. Aluminum oxide disk, Soflex and Super-Snap, made smoother surface than diamond bur, M.F.S., or silicon point, Composite polishing kit. 5. The roughness values of surface polished by M.F.S. composed of diamond burs, were less than those of Composite polishing kit made from silicone points.

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Impact of PSI-KIT Nitriding model on hypothetical Spent Fuel Pool accident simulation

  • Mateusz Malicki;Terttaliisa Lind
    • Nuclear Engineering and Technology
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    • v.55 no.7
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    • pp.2504-2515
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    • 2023
  • In past years the Paul Scherrer Institute (PSI, Switzerland) and the Karlsruhe Institue of Technology (KIT, Germany)) collaborated to develop a model to account for the active role of nitrogen in the air oxidation of a Zircalloy cladding. The "PSI-KIT Nitriding Model for Zirconium based Fuel Cladding" model was implemented at PSI into PSI-MELCOR 1.8.6. In order to make a preliminary evaluation of the effect of the new model on the evolution of full-scale spent fuel pool accidents, one spent fuel pool event was analyzed using the PSI research version of PSI-MELCOR 1.8.6, which includes the nitriding model. To adapt an existing input deck for the calculations, a sensitivity study was conducted to find an optimal nodalization for the analyses. The nitriding model results were compared to those calculated with the MELCOR 1.8.6-PSI without the new nitriding model. The results demonstrate the effect of the nitriding reactions in spent fuel pool accident progression. Moreover, they confirm the impact of ZrN formation during cladding oxidation in air when the oxidation reactions lead to oxygen starvation inside the fuel assemblies. The nitriding reaction led to higher chemical heat generation during the accident and to an earlier failure of the cladding than when the effect of nitrogen reactions was not considered. It should be noted that the nitriding model, as implemented in the PSI version of MELCOR 1.8.6 has not yet been conclusively validated. Thereby the results presented in this paper should be treated as a preliminary demonstration of the capabilities of the model.

Reliability Design of the Hinge Kit System in Common Refrigerator Under Repetitive Load (상용 냉장고에서 반복 하중을 받는 힌지 키트 시스템(HKS)의 신뢰성 설계 연구)

  • Woo, Seong-woo;Lee, Jongkil
    • 대한공업교육학회지
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    • v.33 no.2
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    • pp.312-324
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    • 2008
  • To improve the newly designed HKS(hinge kit system) in common refrigerators, it was investigated the new robust methodologies. There were the study of failure modes, mechanisms in the marketplace, and the design parameters of HKS with various improvements using accelerated life testing. Based on the claimed marketplace product returns and 1st ALT reproduction, the fracturing and cracking occur in the housing of the HKS. The missing design parameters of the failed HKS in the design phase of the refrigerator was the housing hinge kit structure. The corrective action plans are the modifications of the housing hinge kit structure from the open supporting to all supporting structure. Based on 2nd ALTs, the fracturing and cracking occur in the torsion shaft. The missing design parameter was the roundness of torsion shaft. After a sequence of ALT testing, the levels of the missing design parameters were setup. The yearly failure rate and B1 life of the redesigned HKS, based on the results of ALT, were over 0.01 percent and 10 years, respectively. The parameter design through the inspection of the failed product, load analysis, and three rounds of ALT, was very effective in the new robust design methodologies of the mechanical system and this method can be applied to other design system.

Development of a 3-axis fine positioning stage : Part 2. Experiments and performance evaluation (초정밀 3축 이송 스테이지의 개발 :2. 동특성 실험 및 성능 평가)

  • Kang, Joong-Ok;Kim, Man-Dal;Baek, Seok;Han, Chang-Soo;Hong, Sung-Wook
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 2003.06a
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    • pp.1207-1210
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    • 2003
  • This paper deals with experiments for dynamic characteristics and performance evaluation of the 3-axis fine positioning stage developed in [1]. The features of the developed fine positioning stage are the long stroke due to the magnetically preloaded PZT actuators, the minimum motion crosstalk due to the use of a ball contact mechanism and the compact design. The dynamic characteristics of the actuator and the stage are tested with the preload changed in order to validate the actuator and the stage design. Performance evaluation is also made for the PZT actuators as well as the stage positioning accuracy. Experimental results show that the developed stage is accurate enough to be used for nanometer positioning.

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Exergy Analysis of Regenerative Gas Turbine Systems with Afterfogging (압축기 출구 물분사가 있는 재생 가스터빈 시스템의 엑서지 해석)

  • Kim, Kyoung-Hoon;Ko, Hyung-Jong;Kim, Se-Woong
    • Journal of the Korean Society of Industry Convergence
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    • v.13 no.1
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    • pp.31-39
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    • 2010
  • An exergy analysis is carried out for the regenerative gas turbine cycle which has a potential of enhanced thermal efficiency and specific power owing to the more possible water injection than that of inlet fogging under the ambient conditions. Using the analysis model in the view of the second law of thermodynamics, the effects of pressure ratio, water injection ratio and ambient temperature are investigated on the performance of the system such as exergetic efficiency, heat recovery ratio of recuperator, exergy destruction or loss ratios, and on the optimal conditions for maximum exergy efficiency. The results of computation for the typical cases show that the regenerative gas turbine system with afterfogging can make a notable enhancement of exergy efficiency.

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Design Optimization Based on Designer's Preferences for the Mean and Variance (평균과 분산에 관한 설계자 선호에 기초한 설계 최적화)

  • Park, Jong-Cheon;Kim, Kyung-Mo;Kim, Kwang-Ho
    • Journal of the Korean Society of Industry Convergence
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    • v.12 no.1
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    • pp.35-42
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    • 2009
  • In Taguchi's quadratic expected loss function used as robustness metric of performance characteristics, the mean and variance contributions are confounded. The consolidation of the mean and variance in the expected loss function may not always be the ideal approach. This paper presents a procedure for multi-attributes design optimization, where the mean and variance of performance characteristics are considered as separate attributes having designer's relative preferences for them and Technique for Order Preference by Similarity to Ideal Solution(TOPSIS) is introduced to attain robust optimal design. The effectiveness of proposed approach is shown with an example of a weld line minimization problem in the injection molding process.

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A New ELISA Kit Based on Antigenic Epitopes for Diagnosing Brucella abortus

  • Khodabakhsh, Tannaz;Arabi, Azin;Pakzad, Parviz;Gheflat, ShivaSadat;Bahreinipour, Akram;Bandehpour, Mojgan
    • Microbiology and Biotechnology Letters
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    • v.47 no.1
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    • pp.158-163
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    • 2019
  • Brucellosis is one of the most important zoonotic diseases that lead to a great amount of economic losses. Prevention and diagnosis are both necessary to eradicate this disease. The identification and evaluation of different antigens of Brucella spp. play a key role in the progress of diagnostic programs. In this study, we designed, produced, and evaluated a 24-kDa polypeptide containing antigenic epitopes of VirB2, 3, and 9 of Brucella abortus for use with the ELISA kit. The produced polypeptide is appropriate for diagnosing brucellosis in bovines by a laboratory diagnostic kit, with 100% sensitivity and 97.5% specificity.

Correlation Between Skin Prick Test and Enzyme-linked Immunosorbent Assay Using Serum for Identification of Subjects Positive to Major Indoor Respiratory Allergens (실내환경 주요 호흡기 알레르기 항원 감작검사를 위한 피부단자 시험과 혈청 ELISA시험의 상관성에 대한 연구)

  • Heo, Yong;Kim, Hyoung-Ah
    • Journal of Environmental Health Sciences
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    • v.34 no.5
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    • pp.369-373
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    • 2008
  • The indoor environment is an important source of exposure to various aeroallergens and pathogenic microorganism. It has been shown that exposure to aeroallergens enhances the risk of indoor inhabitants developing asthma. Since the skin prick test, a typical clinical method for identification of subjects positive to allergens, can rarely cause fatal or non-fatal reactions in susceptible persons, an in vitro assay such as ELISA using serum has been considered for testing positivity against various allergens. We evaluated the validity of a serum ELISA kit for screening positive subjects to major aeroallergens including Dermatophagoides farinae, Dermatophagoides pteronyssinus, cockroach, Alternaria, Cladosporium, Aspergillus, Penicillium, dog hair, cat fur, mugwort, and ragweed. The ELISA results were compared with the skin prick test results, and sensitivity, specificity, and overall accuracy were calculated to each allergen. Higher sensitivities were obtained from D. farinae, (77.8%) and D. pteronyssinus (69.2%), but sensitivities to Aspergillus, Penicillium, dog hair, cat fur, and ragweed were very low down to 0%. Specificity ranged from 88.7% (cat fur) to 100% (mugwort and ragweed). Overall the accuracy of the serum ELISA kit was relatively high, in that the lowest was 85.1% for cat fur and the highest was 98.6% for Alternaria, Cladosporium, and ragweed. Considering specificity and overall accuracy for the serum ELISA kit, it may be considered reliable. However, when the kit is used for screening purpose, positivity to aeroallergens should be carefully determined since sensitivity for the kit is low.

Effects of gamma-aminobutyric acid and piperine on gene regulation in pig kidney epithelial cell lines

  • Shin, Juhyun;Lee, Yoon-Mi;Oh, Jeongheon;Jung, Seunghwa;Oh, Jae-Wook
    • Asian-Australasian Journal of Animal Sciences
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    • v.33 no.9
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    • pp.1497-1506
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    • 2020
  • Objective: Gamma-aminobutyric acid (GABA) and piperine (PIP) are both nutritional supplements with potential use in animal diets. The purpose of this study is to investigate the effect of GABA and/or PIP treatment on the gene expression pattern of a pig kidney epithelial cell line. Methods: LLCPK1 cells were treated with GABA, PIP, or both, and then the gene expression pattern was analyzed using microarray. Gene ontology analysis was done using GeneOntology (Geneontology.org), and validation was performed using quantitative real-time polymerase chain reaction. Results: Gene ontology enrichment analysis was used to identify key pathway(s) of genes whose expression levels were regulated by these treatments. Microarray results showed that GABA had a positive effect on the transcription of genes related to regulation of erythrocyte differentiation and that GABA and PIP in combination had a synergistic effect on genes related to immune systems and processes. Furthermore, we found that effects of GABA and/or PIP on these selected genes were controlled by JNK/p38 MAPK pathway. Conclusion: These results can improve our understanding of mechanisms involved in the effect of GABA and/or PIP treatment on pig kidney epithelial cells. They can also help us evaluate their potential as a clinical diagnosis and treatment.