• 천문학회보
• /
• 제41권2호
• /
• pp.52.3-53
• /
• 2016

일반적으로 사용되는 소구경 망원경은 경통에 의한 차폐로 인해 내부 구조를 보기 쉽지 않으므로, 망원경 광학계를 이해하기에는 적합하지 않다. 본 연구에서는 최소한의 배플 만을 사용하여 경통이 없는 구조의 개방형 망원경을 설계 및 제작하였다. 개발된 변환식 반사망원경 키트(TRT Kit, Transformable Reflecting Telescope Kit)는 부경 모듈을 교체하는 방식만으로 뉴턴식 망원경(Newtonian Telescope), 카세그레인식 망원경(Cassegrain Telescope), 그리고 그레고리식 망원경(Gregorian Telescope)으로 변형하는 것이 가능하다. 주경, 부경을 비롯한 망원경의 모든 부분은 사용자가 직접조립할 수 있도록 모듈화(Modularization) 하였다. 또한 부경에 부착된 슬라이딩 장치 및 리니어 스테이지(Linear Stage)는 망원경의 초점을 정밀하게 맞출 수 있도록 설계하였다. TRT Kit를 이용하여 학생들은 세 가지 형태의 망원경 광학계를 직접 조립하고 그 구조 및 성능을 비교해 볼 수 있으며, 광축 정렬, 정밀 초점 조절 과정을 통해 기본적인 광학계의 원리를 이해 할 수 있다.

• Asian-Australasian Journal of Animal Sciences
• /
• 제33권9호
• /
• pp.1497-1506
• /
• 2020

Objective: Gamma-aminobutyric acid (GABA) and piperine (PIP) are both nutritional supplements with potential use in animal diets. The purpose of this study is to investigate the effect of GABA and/or PIP treatment on the gene expression pattern of a pig kidney epithelial cell line. Methods: LLCPK1 cells were treated with GABA, PIP, or both, and then the gene expression pattern was analyzed using microarray. Gene ontology analysis was done using GeneOntology (Geneontology.org), and validation was performed using quantitative real-time polymerase chain reaction. Results: Gene ontology enrichment analysis was used to identify key pathway(s) of genes whose expression levels were regulated by these treatments. Microarray results showed that GABA had a positive effect on the transcription of genes related to regulation of erythrocyte differentiation and that GABA and PIP in combination had a synergistic effect on genes related to immune systems and processes. Furthermore, we found that effects of GABA and/or PIP on these selected genes were controlled by JNK/p38 MAPK pathway. Conclusion: These results can improve our understanding of mechanisms involved in the effect of GABA and/or PIP treatment on pig kidney epithelial cells. They can also help us evaluate their potential as a clinical diagnosis and treatment.

• Toxicological Research
• /
• 제31권1호
• /
• pp.69-75
• /
• 2015

The objective of this study was to investigate the toxicological information of freeze-dried powder from Allomyrina dichotoma (A. dichotoma) larvae as a food ingredient. The powder, suspended in distilled water, was administered once daily by oral gavage to four groups of Sprague-Dawley (SD) rats at dose levels of 0 (vehicle control), 250, 850, and 2500 mg/kg/day. After 13 wks of repeated administration, the standard toxicological parameters such as mortality, clinical signs, body weight, food consumption, ophthalmologic examination, clinical pathology, organ weights and macro/microscopic examination were applied for assessment of general toxicity. In addition, serum IgE and histamine levels were determined to evaluate allergenicity. The freeze-dried powder from A. dichotoma larvae did not produce treatment-related changes or findings in any toxicological parameters in either sex of any dosed groups except for slight increases in serum histamine levels at 2500 mg/kg/day. The changes were considered not to be adverse since the magnitude was minimal. In conclusion, the NOAEL (No Observed Adverse Effect Level) of the freeze-dried powder from A. dichotoma larvae was determined to be 2500 mg/kg/day or more in both sexes of SD rats and it is considered a candidate to be edible material.

• Parasites, Hosts and Diseases
• /
• 제52권4호
• /
• pp.377-381
• /
• 2014

Microscopy is considered as the gold standard for malaria diagnosis although its wide application is limited by the requirement of highly experienced microscopists. PCR and serological tests provide efficient diagnostic performance and have been applied for malaria diagnosis and research. The aim of this study was to investigate the diagnostic performance of nested PCR and a recently developed an ELISA-based new rapid diagnosis test (RDT), NovaLisa test kit, for diagnosis of malaria infection, using microscopic method as the gold standard. The performance of nested-PCR as a malaria diagnostic tool is excellent with respect to its high accuracy, sensitivity, specificity, and ability to discriminate Plasmodium species. The sensitivity and specificity of nested-PCR compared with the microscopic method for detection of Plasmodium falciparum, Plasmodium vivax, and P. falciparum/P. vivax mixed infection were 71.4 vs 100%, 100 vs 98.7%, and 100 vs 95.0%, respectively. The sensitivity and specificity of the ELISA-based NovaLisa test kit compared with the microscopic method for detection of Plasmodium genus were 89.0 vs 91.6%, respectively. NovaLisa test kit provided comparable diagnostic performance. Its relatively low cost, simplicity, and rapidity enables large scale field application.

• 대한수의학회지
• /
• 제36권3호
• /
• pp.731-741
• /
• 1996

ELISA kit for cortisol was developed and then evaluated. Polyclonal antihydrocortisone-3-(o-carboxymethyl)oxime BSA rabbit serum was used to coat the 96-well microplates. The minimum detection limit of the kit was 250pg of cortisol per milliliter. The within-run variation and the day to day variation of the ELISA system were 2.0 and 5.9 at maximum, respectively. The kit was used to determine whether salivary cortisol concentration could replace blood cortisol concentration in dexamathasone suppression test of dogs. Changes of cortisol concentration were measured in serum or saliva after intravenous administration of 0.01mg of dexamethasone per kilogram of body weight. Blood alone, saliva alone or both were collected at 0, 30, 60, 120, 240, and 360 minutes after injection of dexamethasone. The change in blood cortisol concentration was found to be suitable in dexamathasone suppression test of dogs, but the change in salivary cortisol concentration was not. The kit was also used to determine whether salivary cortisol concentration could be a stress index as well as blood cortisol concentration in dogs. Two types of trial were performed to estimate the stress either by blood or salivary cortisol concentration. The first trial was stress experiment by intravenous injection of 0.2IU of PZI-insulin per kilogram body weight. Either blood alone or saliva alone was collected at 0, 30, 60, and 90 minutes after insulin administration. Both blood and salivary cortisol concentration were found to be suitable index in estimating stress from hypoglycemia by injection of insulin. The second trial was stress experiment by electrical irritation. The dogs were irritated with anti-bark device for 10 seconds. Blood was collected before and at 2 and 5 minutes after electrical irritation. Saliva was collected before and at 3 and 6 minutes after electrical irritation. The blood cortisol concentration, but not the salivary cortisol concentration was found to be suitable index in estimating stress from electrical irritation. Cushing syndrome in a dog was also successfully diagnosed with this kit.

• 한국항공우주학회지
• /
• 제35권3호
• /
• pp.260-267
• /
• 2007

GAK(GPS adapter kit)은 GPS/INS 항법 모듈을 내장한 사거리 연장 키트의 일종으로 자유 낙하식 폭탄에 장착하여 투하 정확도의 향상을 목적으로 한다. 본 논문에서는 GAK 항법 모듈의 차량 시험 결과를 제시하였다. 항법 모듈은 상용의 MEMS IMU, 내장형 GPS 수신기 및 항법 컴퓨터로 구성된다. GPS 수신기는 위성 가시성 향상 및 자세 측정을 위하여 다중 안테나를 사용하는 구조로 설계하였다. 실시간 항법 소프트웨어는 모듈화 구조로 설계하여 유지보수성 및 확장성을 고려하였다. 항법 모듈의 성능을 평가하기 위해 고성능 INS인 Honeywell H-726 MAPS를 탑재한 차량 시험을 수행하였다. 차량 시험 결과 GPS 자세 결과를 사용한 GAK 통합 합법 모듈이 일반적인 GPS/INS 통합의 경우에 비하여 더 나은 항법 성능을 나타내었으며 재밍 환경을 고려한 경우에도 순수 항법에 비하여 우수한 coasting 성능을 제공하였다.

• 한국축산식품학회지
• /
• 제33권2호
• /
• pp.198-204
• /
• 2013

This study aimed to apply the Parallux system to detect (fluoro)quinone antibiotics residues in raw bovine milk. The immunogen enabled the generation of a specific antiserum with a titer of 1/40,000. The $Parallax^{TM}$ kit using the antibody displayed $IC_{50}$ value of 10 to 150 ppb for (fluoro)quinolone antibiotics. $Parallax^{TM}$ kit was also sensitive for the detection of incurred (fluoro)quinolone at Korean Maximum Residual Levels in raw bovine milk as the result of dose response test. Cross reactivities of the antibody with the common (fluoro)quinolones were determined to be norfloxacin, 100%; enrofloxacin, 100%; ciprofloxacin, 100%; danofloxacin, 100%; nalidixic acid, 40%. Lower detection limit (LOD) values of the $Parallax^{TM}$ kit in raw bovine milk were determined to be norfloxacin, 4 ppb; enrofloxacin, 5 ppb; danofloxacin, 5 ppb; ciprofloxacin, 5 ppb and nalidixic acid, 10 ppb. The $Parallax^{TM}$ kit was run 8 times with five different concentrations of norfloxacin to determine the coefficient of variation (CV, %) of intra-assay, which was between 2.7% and 11.8%. To confirm the precision among kit batches for the inter-assay, five different batch kits were tested with 2 different concentration of norfloxacin. The CVs of the inter assay were 4.2% at 50 ppb, and 7.2% at 10 ppb norfloxacin, respectively.

• 공학교육연구
• /
• 제24권5호
• /
• pp.38-45
• /
• 2021

The CanSat was designed as an educational satellite simulation program that implements the overall system of the satellite such as the command processing unit, the communication unit, and the power unit in a structure of the size of a can. In particular, the training effect is very excellent because the trainee can learn a process similar to the actual satellite development process by designing, manufacturing, testing, and launching. Republic of Korea Air Force Academy has been using the CanSat production kit used by the domestic can satellite contest experience department for education, but since it was produced based on PCB, it was impossible to show creativity and operation was restricted even with small mistakes. In this paper, we analyze the existing CanSat kit and propose a new educational CanSat kit that can be used in creative engineering design and practice subjects that will be reorganized into a regular course from 2021, and a lesson plan. In conclusion, by using the proposed CanSat kit for lectures, it is possible to achieve educational purposes and effects, improve lecture satisfaction, and provide stable instruction.

• 열처리공학회지
• /
• 제32권4호
• /
• pp.168-174
• /
• 2019

The microstructural features and relative room temperature mechanical properties were investigated in various compositions of Mg-xAl-yZn alloys by thxiomolding process. The microstructure was composed of ${\alpha}$-Mg particles and mixture of ${\alpha}$-Mg and ${\beta}-Mg_{17}Al_{12}$ eutectic phase. The amount of ${\beta}-Mg_{17}Al_{12}$ eutectic phase in mixture was increased with increasing Al and Zn contents without grain refinement. After adding Ti content, however, the morphology of ${\beta}-Mg_{17}Al_{12}$ eutectic phase transformed from net-like to discontinuous shape and the average grain size reduced. To determine the relationship between microstructural features and their mechanical properties, a tensile test was performed at room temperature. As a result, it was found that the mechanical properties were improved in all of Ti contained alloys due to increased elongation and the mechanisms are discussed in terms of microstructural evolution.

• 한국재료학회지
• /
• 제29권4호
• /
• pp.258-263
• /
• 2019

Since the directly bonded interface between TiAl alloy and SCM440 includes lots of cracks and generated intermetallic compounds(IMCs) such as TiC, FeTi, and $Fe_2Ti$, the interfacial strength can be significantly reduced. Therefore, in this study, Cu is selected as an insert metal to improve the lower tensile strength of the joint between TiAl alloy and SCM440 during friction welding. As a result, newly formed IMCs, such as $Cu_2TiAl$, CuTiAl, and $TiCu_2$, are found at the interface between TiAl alloy and Cu layer and the thickness of IMCs layers is found to vary with friction time. In addition, to determine the relationship between the thickness of the IMCs and the strength of the welded interfaces, a tensile test was performed using sub-size specimens obtained from the center to the peripheral region of the friction-welded interface. The results are discussed in terms of changes in the IMCs and the underlying deformation mechanism. Finally, it is found that the friction welding process needs to be idealized because IMCs generated between TiAl alloy and Cu act to not only increase the bonding strength but also form an easy path of fracture propagation.