• Title/Summary/Keyword: KIST

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김치유래 젖산균의 균체지방산 분석을 이용한 분류학적 연구

  • Lee, Jung-Sook;Jung, Min-Chul;Kim, Woo-Sik;Lee, Keun-Chul;Kim, Hong-Joong;Park, Chan-Sun;Lee, Hun-Joo;Joo, Yun-Jung;Lee, Kun-Jong;Ahn, Jong-Seog;Park, Wan;Park, Yong-Ha;Mheen, Tae-Ick
    • Microbiology and Biotechnology Letters
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    • v.24 no.2
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    • pp.234-241
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    • 1996
  • Two hundreds and thirty lactic acid bacteria, mostly isolated from Kimchi, including type strains were used for analysis of cellular fatty acids. The 230 test strains were recoverd in 7 major and 1 single clusters defined at Euclidian distance of 17.5. These aggregate taxa were equivalent to the genus Leuconostoc (aggregate group A, B, C and D), the genus Lactobacillus (aggregate group F), the genera Lactobacillus and Pediococcus (aggregate group E) and the genera Leuconostoc and Lactobacillus (aggregate group G). It is concluded as evident that FAMEs (Fatty Acid Methyl Esters) profile of cell can be used as a criterion in classification of lactic acid bacteria from kimchi. Additional comparative taxonomic studies need to be carried out on well chosen representative strains to determine the most appropriate methods of value.

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A New Evaluation Model for Natural Attenuation Capacity of a Vadose Zone Against Petroleum Contaminants (유류 오염물질에 대한 불포화대 자연 저감능 등급화 기법 개발)

  • Woo, Heesoo;An, Seongnam;Kim, Kibeum;Park, Saerom;Oh, Sungjik;Kim, Sang Hyun;Chung, Jaeshik;Lee, Seunghak
    • Journal of Soil and Groundwater Environment
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    • v.27 no.spc
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    • pp.92-98
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    • 2022
  • Although various methods have been proposed to assess groundwater vulnerability, most of the models merely consider the mobility of contaminants (i.e., intrinsic vulnerability), and the attenuation capacity of vadose zone is often neglected. This study proposed an evaluation model for the attenuation capacity of vadose zone to supplement the limitations of the existing index method models for assessing groundwater vulnerability. The evaluation equation for quantifying the attenuation capacity was developed from the combined linear regression and weighted scaling methods based on the lab-scale experiments using various vadose zone soils having different physical and biogeochemical properties. The proposed semi-quantifying model is expected to effectively assess the attenuation capacity of vadose zone by identifying the main influencing factors as input parameters together with proper weights derived from the coefficients of the regression results. The subsequent scoring and grading system has great versatility while securing the objectivity by effectively incorporating the experimental results.

Screening of the Angiotensin II Antagonists from Medicinal Plants (생약의 안지오텐신 II 수용체 결합저해활성 검색)

  • Oh, Won-Keun;Kang, Dae-Ook;Park, Chan-Sun;Ahn, Soon-Cheol;Ko, Hack-Ryong;Kim, Bo-Yeon;Mheen, Tae-Ik;Ahn, Jong-Seog;Lee, Hyun-Sun
    • Korean Journal of Pharmacognosy
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    • v.28 no.1
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    • pp.26-34
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    • 1997
  • The total MeOH extracts of 149 herbal medicines were screened far angiotensin ll receptor antagonistic activity using rat liver membrane fraction. Four medicinal plants- Atractylodes japonica, Evodia officinalis, Scutellaria baicalensis, Machilus thunbergii showed strong angiotensin ll antagonistic activity.

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F1064m: New Inhibitor of Cholesteryl Ester Transfer Protein (CETP), Isolated from Gliocladium virens F1064

  • Kwon, Byoung-Mog;Cho, Joung-Suk;Jeong, Tae-Sook;Kim, Sung-Uk;Son, Kwang-Hee;Kim, Young-Kook;Nam, Ji-Youn;Han, Kyu-Hoon;Bok, Song-Hae
    • Proceedings of the Korean Society of Applied Pharmacology
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    • 1995.04a
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    • pp.65-65
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    • 1995
  • Cholesteryl Ester Transfer Protein(CETP), a hydrophobic glycoprotein with molecular mass 74KDa, is a lipid transfer protein found in plasma which mediates the transfer of cholesterol ester and triglyceride between high-density lipoprotein (HDL) and other lipoproteins, therefore, it might influence HDL levels. The lipoprotein profile associated with human CETP deficiency (that are two Japanese families, high HDL and low LDL) has low atherogenic potential, raising the possibility that CETP inhibitors can be used as antiatherosclerotic drugs.

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F93-A: A Inhibitor of Farnesyl Protein Transferase from Aspergillus fumigatus KL93

  • Kwon, Byoung-Mog;Lee, Seung-Ho;Jeong, Tae-Sook;Kim, Sung-Uk;Son, Kwang-Hee;Park, Diol;Kim, Young-Kook;Nam, Ji-Youn;Bok, Song-Hae
    • Proceedings of the Korean Society of Applied Pharmacology
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    • 1995.04a
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    • pp.66-66
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    • 1995
  • Mutated forms of the ras oncogenes are associated with about 30% of human tumors. The ras genes encode 21KDa proteins, called p21 or Ras, that are associated with the plasma membrane. FPTase is a dimeric enzyme that catalyses the transfer of the farnesyl group from farnesyl pyrophosphate onto cysteine 186 at the C-terminus of the Ras protein. This is mandatory process for triggering ras oncogene toward tumor formation. Therefore, selective inhibitors of FPTase have the potential to be used as antitumorgenic agents.

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Genome-Based Reclassification of Strain KIST612, Previously Classified as Eubacterium limosum, into a New Strain of Eubacterium callanderi

  • Ji-Yeon Kim;Byeongchan Kang;Soyoung Oh;Yeji Gil;In-Geol Choi;In Seop Chang
    • Journal of Microbiology and Biotechnology
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    • v.33 no.8
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    • pp.1084-1090
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    • 2023
  • The strain KIST612, initially identified as E. limosum, was a suspected member of E. callanderi due to differences in phenotype, genotype, and average nucleotide identity (ANI). Here, we found that E. limosum ATCC 8486T and KIST612 are genetically different in their central metabolic pathways, such as that of carbon metabolism. Although 16S rDNA sequencing of KIST612 revealed high identity with E. limosum ATCC 8486T (99.2%) and E. callanderi DSM 3662T (99.8%), phylogenetic analysis of housekeeping genes and genome metrics clearly indicated that KIST612 belongs to E. callanderi. The phylogenies showed that KIST612 is closer to E. callanderi DSM 3662T than to E. limosum ATCC 8486T. The ANI between KIST612 and E. callanderi DSM 3662T was 99.8%, which was above the species cut-off of 96%, Meanwhile, the ANI value with E. limosum ATCC 8486T was not significant, showing only 94.6%. The digital DNA-DNA hybridization (dDDH) results also supported the ANI values. The dDDH between KIST612 and E. callanderi DSM 3662T was 98.4%, whereas between KIST612 and E. limosum ATCC 8486T , it was 57.8%, which is lower than the species cut-off of 70%. Based on these findings, we propose the reclassification of E. limosum KIST612 as E. callanderi KIST612.

Phenolic Wastewater Treatment by a Mixed Culture GE2 Immobilized on Activated Carbon

  • Oh, Hee-Mock;Ku, Young-Hwan;Ahn, Keuk-Hyon;Kwon, Gi-Seok;Kho, Yung-Hee;Mheen, Tae-Ick;Yoon, Byung-Dae
    • Journal of Microbiology and Biotechnology
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    • v.6 no.2
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    • pp.116-119
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    • 1996
  • The biological treatment by a mixed culture GE2 immobilized on activated carbon was investigated with a phenolic resin industrial wastewater containing 41,000 mg/l of phenol and 2,800 mg/l of formaldehyde. At a dilution of 20 times with aerated tap water, influent and effluent $COD_{Mn}$ were 4,587 mg/l and 46 mg/l, that is, $COD_{Mn}$ removal efficiency was 99.0%. At this time, phenol and formaldehyde con-centration of the effluent were 1.24 and 6.80 mg/l, indicating removal efficiencies of 99.9 and 94.1%, respectively.

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