• Journal of Applied Biological Chemistry
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• 제61권4호
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• pp.411-416
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• 2018

Chlorpyrifos (CPF) is one of the most heavily used organophosphate pesticides and is useful as an insecticide drug. However, CPF also causes toxic effects in nontarget organisms, including humans and animals. Jageum-Jung (JGJ) is a traditional oriental medicine, composed of five specific herbs with antioxidant and hepatoprotective properties, used for detoxification. In the present study, highly concentrated CPF was orally administrated to male Institute of Cancer Research mice to produce acute toxicity, and the protective effects of JGJ administration were investigated through statistical analysis of changes in body and organ weights and serum biochemical parameters. JGJ caused body and organ weights to recover and reduced the levels of serum biochemical parameters indicative of liver damage, such as glutamic oxalate transaminase, glutamic pyruvate transaminase, alkaline phosphatase, lactic dehydrogenase, urea, glucose, total cholesterol, and triglyceride, that had been increased by CPF treatment. Our results demonstrated that JGJ ameliorates the effects of acute chlorpyrifos-induced toxicity. Therefore, JGJ has the potential to be used as a traditional medicine to alleviate insecticide toxicity.

• 대한전자공학회논문지SP
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• 제46권3호
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• pp.112-118
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• 2009

피부색 검출 기법은 안면 정보를 이용한 체질 진단 및 건강 진단, 인간과 로봇과의 상호작용, 영상 검색 시스템 등 다양한 응용분야에서 사람의 얼굴과 손의 검출을 위해 많이 사용되어 왔다. 비디오 영상의 경우 조명이나 환경 변화에 강인한 피부색 영역의 추적을 위해 매 프레임마다 대상 영역의 피부색 모델을 업데이트 하는 것이 일반적이나, 단일 영상에서 피부색 영역을 검출하거나 비디오 영상의 첫 프레임에서 피부색 영역을 검출할 때에는, 많은 연구들이 하나의 고정된 피부색 모델을 이용하기 때문에 입력 영상의 특징에 따라 낮은 검출율이나 높은 긍정 오류율이 발생하는 경우가 많다. 이러한 문제점을 해결하기 위해 본 논문에서는 피부색 검출 결과를 피드백 받아 피드백 받은 정보를 바탕으로 피부색 검출 조건을 수정하는 과정을 반복함으로써 다양한 환경 조건들을 가지는 단일 영상에 대해 효과적으로 피부색을 검출할 수 있는 방법을 제안한다.

• KSBB Journal
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• 제29권2호
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• pp.124-130
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• 2014

Acer tegmentosum was a traditional Korean herbal medicine showing various pharmacological activities. In this work, A. tegmentosum were extracted with boiling water and then successively partitioned with dichloromethane, ethyl acetate, n-butyl alcohol (n-BuOH), and water. Salidoside, the target compound, was purified in n-BuOH phase using a chromatography method. For the analysis of salidoside, TLC and LC-MS were used as well as on-line screening $HPLC-ABTS^+$ assay with three different wavelength of 254, 280, and 320 nm. An amount of 1.34 g of salidoside were obtained in n-BuOH phase fromAcer tegmentosum was a traditional Korean herbal medicine showing various pharmacological activities. In this work, A. tegmentosum were extracted with boiling water and then successively partitioned with dichloromethane, ethyl acetate, n-butyl alcohol (n-BuOH), and water. Salidoside, the target compound, was purified in n-BuOH phase using a chromatography method. For the analysis of salidoside, TLC and LC-MS were used as well as online screening $HPLC-ABTS^+$ assay with three different wavelength of 254, 280, and 320 nm. An amount of 1.34 g of salidoside were obtained in n-BuOH phase from 3 kg of dry biomass. The on-line screening $HPLC-ABTS^+$ assay is rapid and efficient tool to search bioactivity from A. tegmentosum. 3 kg of dry biomass. The on-line screening $HPLC-ABTS^+$ assay is rapid and efficient tool to search bioactivity from A. tegmentosum.

• 센서학회지
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• 제26권2호
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• pp.141-147
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• 2017

A pressure sensor in pulse measurement system is a core component for precisely measuring the pulse waveform of radial artery. A pulse sensor signal that measures the pulse wave in contact with the skin is affected by the temperature difference between the ambient temperature and skin surface. In this study, we found experimentally that the signal changes of the pressure sensors and a temperature sensor were caused by the temperature of the wrist surface while the pressure sensor was contacted on the skin surface for measuring pulse wave. To observe the signal change of the pulse sensor caused by temperature increase on sensor surface, Peltier device that can be kept at a set temperature was used. As the temperature of Peltier device was kept at $35^{\circ}C$ (the maximum wrist temperature), the device was put on the pulse sensor surface. The temperature and pressure signals were obtained simultaneously from a temperature sensor and six pressure sensors embedded in the pulse sensor. As a result of signal analysis, the sensor pressure was decreased during temperature increase of pulse sensor surface. In addition, the signal difference ratio of pressure and temperature sensors with respect to thickness of cover layer in pulse sensor was increased exponentially. Therefore, the signal of pressure sensor was modified by the compensation equation derived by the temperature sensor signal. We suggested that the thickness of cover layer in pulse sensor should be designed considering the skin surface temperature.

• 동의생리병리학회지
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• 제18권4호
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• pp.1028-1035
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• 2004

Imperatorin, a biologically active furanocoumarin from the roots of Angelica dahurica (Umbelliferae), was mutagenic and induced transformation of mouse fibroblast cell lines, whereas it provided inhibiting effects on mutagenesis and carcinogenesis induced by various carcinogens. Furthermore, it has been suggested that imperatorin may have potential anticarcinogenic effects when administered orally in the diet. In addition to its anticarcinogenic properties, imperatorin has been shown to possess anticancer activities. We investigated the macro scale gene expression analysis on the HL-60 cells treated with imperatorin. Imperatorin (10μM) were used to treat the cells for 6h, 12h, 24h, 48h, and 72h. In a human cDNAchip study of 10,000 genes evaluated 6, 12, 24, 48, 72 hours after treated with imperatorin in HL-60 cells. Hierarchical cluster against the genes which showed expression changes by more than 2 fold. Three hundred eighty six genes were grouped into 6 clusters by a hierarchical clustering algorithm. Pathway analysis using gene microarray pathway prof Her that is a computer application designed to visualize gene expression data on screen representing biological pathways and groupings of genes.

• 한국콘텐츠학회:학술대회논문집
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• 한국콘텐츠학회 2011년도 춘계 종합학술대회 논문집
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• pp.185-186
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• 2011

본 연구의 목적은 미국국립의학도서관(NLM)의 Unified Medical Language System(UMLS)에 한의학의 용어를 등재하는 것이다. 1차 등재 대상으로 한국한의학연구원(KIOM)에서 진행중인 한의학 용어 정제 연구 결과물 중 임(任) 독맥(督脈)과 12정경(正經)에 속하는 360개의 경혈명(經穴名)의 Concept Unique Identify(CUI)를 선정하였다. UMLS Knowledge Source Server (UMLSKS)의 메타시소러스 검색을 통해 UMLS 내 경혈(經穴)용어에 대한 terminology정보를 수집, 분석하였다. 이를 바탕으로 용어간 개념 비교를 통해 UMLS의 경혈(經穴)용어와 KIOM의 경혈(經穴)용어를 매핑하였다. 마지막으로 Rich Release Format(RRF)로 데이터를 저장하고, Unicode Transformation Format-8 (UTF-8)로 인코딩하여 NLM으로 송부하였다. NLM에서 용어 등재에 대한 적합성 여부를 판별한 후 2010AB버젼에 "TKMT2010"이라는 소스명으로 한글로 된 한의학 용어가 최초로 UMLS에 등재되었다. 향후 UMLS와 같은 국제표준의학용어와 연계를 통해 한의학 용어가 다양한 의학용어체계와 상호호완성을 확보하고 표준화, 세계화할 수 있도록 지속적인 연구가 필요하다.

• 한국한의학연구원논문집
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• 제6권1호
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• pp.89-98
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• 2000

Cerebral ischemia, the most prevalent form of clinical stroke, is a medical problem of the first magnitude. Substantial efforts are being made to develop drugs which will protect the brain from the neurodegeneration followed by an ischemic stroke. A key factor in this process is the development of animal models that mimic the neuropathological consequences of stroke. Recently, there is increasing an evidence that free radical is involved in the mechanisms of ischemic brain damage. We investigated the macro scale gene expression analysis on the global ischemia induced by 4-vessel occlusion in Wister rats. The recent availability of microarrays provides an attractive strategy for elaborating an unbiased molecular profile of large number of genes during ischemic injury. This experimental approach offers the potential to identify molecules or cellular pathways not previously associated with ischemia. Ischemia was induced by 4-vessel occlusion for 10 minutes and reperfused again. RNA from sham control brain and time-dependent ischemed brain were hybridized to microarrays containing 4,000 rat genes. 589 genes were found to be at least 2 fold regulated at one or more time points. These survey data provide the foundation studies that should provide convincing proof for ischemia and oxidative stress on gene expression.

• 대한전자공학회논문지SP
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• 제47권1호
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• pp.1-8
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• 2010

얼굴의 윤곽선을 검출하기 위해서는 일반적으로 입력 영상에 직접 동적 윤곽선 모델(Active Contour Model)을 적용하는 방법을 많이 사용한다. 그러나 동적 윤곽선 모델은 초기의 위치 설정과 사용되는 에너지 함수의 계수 값에 따라 성능에 영향을 받기 때문에, 다양한 조명조건과 환경조건에 따라 최적화된 파라미터들을 설정해야 하는 번거로움이 있다. 또한 섬세한 윤곽선의 검출을 위해서는 모델에서 사용되는 정점의 수를 증가시켜야 하는 단점이 있다. 이러한 단점들을 해결하기 위해, 본 논문에서는 입력영상의 분할된 영역에서의 히스토그램 분석을 통하여, 얼굴 영역과 배경 영역의 픽셀 값을 구분할 수 있는 임계값을 자동으로 찾아, 얼굴의 윤곽선 영역을 검출하는 접근 방법을 제안한다. 제안된 방법은 입력 영상의 분석을 통하여 얼굴의 윤곽선 영역을 검출하기 때문에 다양한 조명과 배경 조건하에서도 높은 성능으로 얼굴의 윤곽선 영역을 검출하였다.

• 한국한의학연구원논문집
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• 제4권1호통권4호
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• pp.27-45
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• 1998

$\ulcorner$Euibangyoochui醫方類聚$\lrcorner$ (1445) is regarded as a treasure-house of the knowledge of traditional oriental medicine which contains over 50,000 prescriptions and enormerous amount of medical information. Despite the importance and information contained in this book, it has been rarely used since it was not convenient to use this book. In this study, therefore, the establishment of database on $\ulcorner$Euibangyoochui$\lrcorner$ was carried out. Before the database establishment of $\ulcorner$Euibangyoochui$\lrcorner$ , basic works such as correction, interpretation, proofreading and translation of original text should be done. The results obtained in this study are summaried as follows : 1) During the course of studying the original text of $\ulcorner$Euibangyoochui$\lrcorner$ , the editing process and transmission of medical books in early Chosun dynasty was figured out. 2) For better correction, interpretation, proofreading and translation of $\ulcorner$Euibangyoochui$\lrcorner$ , $\ulcorner$Euibangyoochui$\lrcorner$ microfilms which are the collection of Japanese Royal Library (宮內廳 圖書寮) were obtained in this study. Through this process, the errors in the republication were able to be corrected. 3) Analyzing the organization and compilatory method of $\ulcorner$Euibangyoochui$\lrcorner$ is one of the basic requirements of understanding the scale of the whole. book and establishing database as a result. So the analysis results were used for the basic structuring of database. 4) $\ulcorner$Euibangyoochui$\lrcorner$ CD- ROM was designed in a way that the images of microfilms, original text and Korean translation can be compared by 3-D device. In addition, the convenience and proficiency of imaging the information and prescriptions of the text is one of the remarkable features of this CD-ROM.

• 동의생리병리학회지
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• 제20권4호
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• pp.853-860
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• 2006

This study was performed to investigate genes which are differently expressed in human blood by administrating of OLT-2. OLT-2 was medical precipitation composed of three medicinal herbs, Ginseng Radix, Astragali Radix, Glycyrrhizae Radix, and anti-leukemia effect of it was evaluated from Byung Hun Jeon of Wonkwang University this study was approved by Institutional Review Board of Korea Institute of Oriental Medicine (Taejeon, Korea) and four male subjects participated in this study. Gene expressions were evaluated by cDNA chip, in which 24,000 genes were spotted. Hierarchical cluster and biological process against the genes, which expression changes were more than 1.6 fold, were constructed by cluster 3.0 providing Stanford University and EASE(http://apps1 .maid.nih.gov/DAVID). Five groups were clustered according to their expression patterns. Group A contained gene decreased by OLT-2 and increased genes by OLT-2 were involved in Group B, C, D. In biological process, expression of genes involved in cytokine or cell calcium signaling, such as interleukin 18 and G-protein beta 4 were increased, but protein tyrosine phosphatase receptor type c, which function is cell adhesion between antigen-presenting cell and T or B-cell, was decreased by OLT-2. This study provides the most comprehensive available survey of gene expression changes in response to anti-leukemia effect of OLT-2 in human blood.