• 한국비블리아학회지
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• 제29권4호
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• pp.319-344
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• 2018

본 연구에서는 오픈사이언스 정책의 일환으로 학문분야별 연구데이터 공유 활용에 관한 인식을 비교분석하였다. 이를 기반으로 과학기술분야 정부출연연구기관 27개 기관 데이터 업무담당자를 대상으로 반구조화된 질문을 통하여 심층인터뷰를 실시하였다. 그 중 데이터 관리 면에서 우수기관 9개 기관을 선정하여 연구데이터 수집 및 관리현황 사례를 구체적으로 제시하였다. 결과 현재 출연연구기관의 연구데이터 수집 관리는 전반적으로 시범사업 단계이며, 데이터의 수집 구축 수준 또한 기관별로 상이하다. 기관별로 살펴보면 수집을 시작하는 단계(KIOM), 수집 관리가 고도화되고 있는 단계(KIST), 공유 활용을 시작하는 단계(KRIBB, KRICT) 등으로 구분된다.

• 대한본초학회지
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• 제39권3호
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• pp.77-84
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• 2024

Objectives : Lysimachia mauritiana Lam. is known as a medicinal plant native to Korea that has antioxidant, anticancer, antibacterial, and antiviral activities. However, until now, research on the cultivation technology of L. mauritiana is insufficient, and there are no research data on the systematic cultivation method and mass production of L. mauritiana. Therefore, this study aims to establish a cultivation system of L. mauritiana. Methods : The cultivation environment of open land and facilities according to the growth of L. mauritiana was compared and tested. In addition, the equivalence of the origin collection extract and the cultivation extract was evaluated through Ultra high performance liquid chromatography (UHPLC) patterns analysis according to cultivation and comparison of the effect of inhibiting respiratory inflammation using BEAS-2B human bronchial epithelial cells. Results : The cultivation technology system was established through cultivation research of L. mauritiana raw materials. In addition, as a result of comparing and evaluating the equivalence of cultivated plants and L. mauritiana raw materials for suppressing respiratory inflammation, the same results were confirmed, and the equivalence was confirmed as a result of analyzing the UHPLC pattern with L. mauritiana raw materials. Conclusions : This study suggests that extract from cultivation research of L. mauritiana plants, which are native to Korea, can be used as a health functional food or medicine to improve respiratory health.

• 한국한의학연구원논문집
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• 제3권1호
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• pp.85-104
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• 1997

As one of the chemical and experimental study on cancer treatment in Korea oriental medicine, we investigated the clinical characteristics on the treatment of cancer patients in oriental medical hospital. Furthermore, we carried out the cytotoxicity test of extracts of herbal medicine. The results are as follows: About Clinical Aspects of the Orient낄 Medical Cancer Treatment- (1) Clinical experience is that 420 Oriental medical hospitals were surveyed. Number of cancer patiens treated by one oriental medical doctor was under 10(about 60%)or from 10 to 20(about 30%). (2) Diagnostic Procedure Is that more than 80% of cancer patients were diagnosed by medical doctor of western hospital. (3) Therapeutic Method is that combination of oriental and western medical treatment was used in oriental medical hospital. (4) Relationship between cancer and constitutions is that to the relationship between cancer and constitutions, about 75% responded and most were considered to be Um. About Experimental Study- Toevaluate the direct cytotoxicity of hot water and methanol extracts of 34 oriental medical prescriptions on stomach cancer cell, SRB assay was carried out. (1) The $IC_{50}$ value of water extract of Samsoum was $19{\mu}g/ml$ and that of methanol extract was $15{\mu}g/ml$ so that we confirmed Samsoum has a strong direct cytotoxicity. (2) The $IC_{50}$ values of water extract of Coptidis Rhizoma, methanol extract of Caesalpiniae Lignum and Draba Semen were $10{\mu}g/ml$, $9{\mu}g/ml$ and $12.5{\mu}g/ml$ respectively, so that we confirmed Coptidis Rhizoma, Caesalpiniae Lignum and Drabs Semen have a strong direct cytototoxicity. (3) The $IC_{50}$ lalue of combinatory prescription of Rhei Rhizoma, Forsythie Fructus and Caesalpiniae Lignum was about $40{\mu}g/ml$.

• 한국한의학연구원논문집
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• 제1권1호
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• pp.433-440
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• 1995

Astragali Radix, the roots of Korean Astragalus membranaceus BUNGE (Leguminosae), was extracted and fractionated. One fraction of them exhibited anti-Herpes Simplex (type I) activity ($ED_{50}=3.403\;{\mu}g/ml$).

• 한국한의학연구원논문집
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• 제1권1호
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• pp.209-244
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• 1995

The time therapy in oriental medicine is to reserch physiological activity and pathological variation with time, or time with disease. In that, this therapy is to research the development of disease, progress, diagnosis, treatment, and the meaning of preventive medicine. The theory of time therapy has been developed form heaven-human corresponding principle, and fourtimes of seasons is related with human life. After that, this theory affects in yin-yang five circle principle and organ-meridian match system. The independent development was formed later, but the origin has progressed for a logn times. in relation with time therapy, traditional acupuncture therory suggested five semen. In twenty century, the doctor rheinhold voll suggested energy exchange theory, this theory is based on time therapy, but has partial differance also too. His oppinion is this´ the energy present in the body has a circulation following the acupuncture teaching, i.e., the organs and their meridians are flooded in a certain sequence by this flowing energy. This maximum times of the organs are of physiologic and pathologic significance to man. In next time, we will discuss the indivisual theory in detail, and wil have comparison, and considering too.

• 대한한의학회지
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• 제34권1호
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• pp.170-178
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• 2013

Objectives: Because TKM-related statistics are scattered in year books, white papers, or web services, it is not easy to use. This study aimed to build a TKM statistical database offering one-stop service. Methods: We collected TKM statistics from year books, white papers, and web services using TKM related keywords which came from the advanced study. Then we removed, merged, or split the statistics looking through data and meta-data of statistics to build a database. Results: First, we classified the statistics into 6 categories and found that the service category was 303, product category 37 and so on. Second, we analyzed the source of statistics and found that the Ministry of Health & Welfare and the Korea statistics were the strongest sources. Third, we analyzed the years of statistics and found that the oldest was 1962, 35% were within the past 3 years, and only 14% were over 10 years old. Finally, we found that the 415 statistics were composed of 1,120 items and the average was 2.70. Conclusions: Through this study we found that more sophisticated TKM statistics must be produced and developed specially in the education, research, and administration categories. We hope that the effort of TKM societies is available to eliminate the lack of statistical producer's understanding about TKM.

• Journal of Applied Biological Chemistry
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• 제57권4호
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• pp.301-305
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• 2014

On-line screening high performance liquid chromatography (HPLC)-$ABTS^+$ assay를 이용한 청호로부터 chlorogenic acid, caffeic acid, vitexin, queretin, aremisinin의 항산화 활성을 온라인스크리닝 하였다. 이때 침적방법을 적용한 추출시간과 추출용매 조성으로부터 추출효율을 확인하였다. 이 결과 100% 물 추출물에서 수율이 가장 좋왔고 chloroenic acid의 항산화 활성이 가장 높았다. 또한 on-line screening HPLC-$ABTS^+$ assay 분석은 천연물에서 항산화 활성을 신속하게 탐색하는데 효율적이다.

• 대한한의학회지
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• 제37권3호
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• pp.39-46
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• 2016

Objectives: The present study aimed at investigating the effects of Schisandrae Fructus (SF) on menopause symptoms in ovariectomized mice. Methods: 6 weeks old female mice were ovariectomized (OVX), and the OVX mice were fed with a three doses of SF (25, 100, and 400 mg/kg, respectively) or $17{\beta}$-estradiol (E2, 0.5 mg/kg) 3 times a week for 6 weeks. Body weight was measured at every week and uterus weight was also measured at the end of experiment. Lipids and osteocalcin levels in serum were analyzed. Results: The uterine weight was not changed in SF treated group, while E2 intake increased the reduced uterine weight by OVX. Although Body weight was increased since two weeks after ovariectomy compared with sham operated group, there was no change in SF or E2 treated group compared with OVX. But LDL was significantly reduced in SF (400 mg/kg) or E2 fed mice and SF or E2 treatment decreased enhanced osteocalcin level by OVX. Conclusion: These results indicated that SF would be effective in the improvement of LDL level and osteoporosis on menopause.

• 한방재활의학과학회지
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• 제26권1호
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• pp.49-61
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• 2016

Objectives To analyze the latest trend through the domestic studies of obesity and support further studies and clinical trials. Methods The theses were searched through KIOM OASIS (http://oasis.kiom.re.kr), The Journal of Society of Korean Medicine for Obesity Research, The Journal of Korean Medicine Rehabilitation. Then, domestic studies were gathered and classified by year, journal and type. Results 92 theses were included. The theses were constantly published every year. According to the analyzing by journals, the most published journal was The Journal of Society of Korean Medicine for Obesity Research. Through the analysis of type, 19 clinical studies and 11 case reports were found. The result of 43 theses analyzing survey and examination numerical value, the study of relationship between obesity and body index is the most published. Among 19 literary studies, 11 studies were searched domestic thesis, 2 studies were searched foreign thesis, 5 studies were searched domestic and foreign thesis simultaneously. Conclusions We need to effort to supplement the statistical error and the topic needs to be diverse. The sustainable research of The human body-oriented obesity studies is required.

• KSBB Journal
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• 제27권1호
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• pp.33-39
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• 2012

In this study three major curcuminoids in turmeric curcumin (1), demethoxycurcumin (2) and bismethoxycurcumin (3) were efficiently extracted by optimizing extraction condition and simultaneously identified using a fast and reliable RP-HPLC-UV-MS and TLC method. The analysis by the $C_{18}$ column was performed and the UV wavelength was fixed at 425 nm. In this result, the total extraction yield of turmeric (Curcuma longa) was increased with extraction time from 1 to 7 h. So, optimum extraction time is 4 h. Also, the highest yield of extraction amount 0.433g 8.66% was obtained by ultrasonic waves with quarter frequency kHz and an extraction time of 7 h. The experiment method was consistent with theoretical Value $r^2$ = 0.987 (1), 0.997 (2) and 0.998 (3). Moreover, LC-MS analysis provided efficiently molecular weight information of three major curcuminoids in turmeric extracts and high purity (~95%) of the curcuminoids were obtained. This work offers would be useful for chemical and biological studies of natural plants and its products.