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Cultivation, UHPLC Pattern Analysis, and Inhibitory Effect on Respiratory Inflammation of Lysimachia mauritiana Lam.

갯까치수염(Lysimachia mauritiana Lam.)의 재배 및 UHPLC 패턴 분석, 호흡기염증 억제 효과

  • Dong-Seon Kim (KM Science Research Division, KIOM) ;
  • Heung Joo Yuk (KM Science Research Division, KIOM) ;
  • JungMi Kim (Medicinal Crop team, Mungyeong Agricultural Technology Center) ;
  • Chaeseok Koh (Medicinal Crop team, Mungyeong Agricultural Technology Center) ;
  • Younjung Jang (Medicinal Crop team, Mungyeong Agricultural Technology Center) ;
  • Yoon-Young Sung (KM Science Research Division, KIOM)
  • 김동선 (한국한의학연구원 한의과학연구부) ;
  • 육흥주 (한국한의학연구원 한의과학연구부) ;
  • 김정미 (문경시농업기술센터 약용작물팀) ;
  • 고채석 (문경시농업기술센터 약용작물팀) ;
  • 장윤정 (문경시농업기술센터 약용작물팀) ;
  • 성윤영 (한국한의학연구원 한의과학연구부)
  • Received : 2024.04.09
  • Accepted : 2024.05.25
  • Published : 2024.05.30

Abstract

Objectives : Lysimachia mauritiana Lam. is known as a medicinal plant native to Korea that has antioxidant, anticancer, antibacterial, and antiviral activities. However, until now, research on the cultivation technology of L. mauritiana is insufficient, and there are no research data on the systematic cultivation method and mass production of L. mauritiana. Therefore, this study aims to establish a cultivation system of L. mauritiana. Methods : The cultivation environment of open land and facilities according to the growth of L. mauritiana was compared and tested. In addition, the equivalence of the origin collection extract and the cultivation extract was evaluated through Ultra high performance liquid chromatography (UHPLC) patterns analysis according to cultivation and comparison of the effect of inhibiting respiratory inflammation using BEAS-2B human bronchial epithelial cells. Results : The cultivation technology system was established through cultivation research of L. mauritiana raw materials. In addition, as a result of comparing and evaluating the equivalence of cultivated plants and L. mauritiana raw materials for suppressing respiratory inflammation, the same results were confirmed, and the equivalence was confirmed as a result of analyzing the UHPLC pattern with L. mauritiana raw materials. Conclusions : This study suggests that extract from cultivation research of L. mauritiana plants, which are native to Korea, can be used as a health functional food or medicine to improve respiratory health.

Keywords

Acknowledgement

본 연구는 한국한의학연구원의 기본사업 '자생식물을 활용한 호흡기 건강 기능성 식품 소재 개발' 과제(과제번호 : KSN20234316)의 지원으로 이루어졌으며, 이에 감사드립니다.

References

  1. Lee Y, Yun N, Kang J, Choi S, Paik JH. The complete chloroplast genome of the medicinal plant Lysimachia mauritiana (Lamarck, 1792). Mitochondrial DNA B Resour. 2022; 7(3): 554-555. doi: 10.1080/23802359.2022.2054738
  2. Roh SS, Seo BI, Park JH. A herbological study on the native plants of Dokdo island. Kor J Herbol. 2012; 27(2): 17-24. doi: 10.6116/kjh.2012.27.2.17
  3. Yasukawa K, Takido M. A flavonol glycoside from Lysimachia mauritiana. Phytochemistry. 1987; 26(4): 1224-1226. https://doi.org/10.1016/S0031-9422(00)82393-6
  4. Bae S, Song YJ. Inhibition of varicella‑zoster virus replication by an ethanol extract of Lysimachia mauritiana. Mol Med Rep. 2017; 15(6): 3847-3851. doi: 10.3892/mmr.2017.6444
  5. Jin SE, Kim JE, Kim SY, Park BJ, Song YJ. An ethanol extract of Lysimachia mauritiana exhibits inhibitory activity against hepatitis E virus genotype 3 replication. J Microbiol. 2017; 55(12): 984-988. doi: 10.1007/s12275-017-7477-1
  6. Pal D. Anti-viral metabolites from medicinal plants (Reference series in Phytochemistry). New York : Springer International Publishing AG; 1st ed. 2024 edition. 2023: 666.
  7. Jwon HS. Iksaeng Yaksul Daejeon (益生養術大全) : An amazing herbal medicine with the efficacy of oriental medicine. Seoul : Academic Editing Center. 2012: 97.
  8. Herbal Medicine Research Society. Herbal medicine encyclopedia. Seoul : Baekman Munhwasa. 2020.
  9. Nam S, Kang S, Kim HJ, Kim J. Effect of substrate volumetric water contents on growth in Lysimachia mauritiana Lam. Hortic Sci Technol. 2017; 35 (Suppl. II): 47.
  10. Jeong E, Kwon S, Choi H, Jeon K, Moon S, Lee SY, Lee CH. Effect of culture condition affecting seed germination and seedling growth of Lysimachia mauritiana Lam. Hortic Sci Technol. 2017; 36 (Suppl. I): 177.
  11. Oh SI, Lee JH, Lee WH, Kang JH, Lee TJ, Lee AK. Analysis of growth, antioxidant concentrations and activity by LED light quality of Lysimachia mauritiana in Korea. Hortic Sci Technol. 2019; 37 (Suppl. I): 151.
  12. Jeong E, Jeon K, Choi H, Moon S, Yeon SH, Lee SY, Kwon S, Lee CH. Several factors affecting cultivation of ground-cover plants on Lysimachia mauritiana Lam. Korea J Plant Res. 2018; 2018: 59.
  13. Boero S, Silvestri M, Ullmann N, Rossi GA. Modulation by flunisolide of tumor necrosis factor-α-induced stimulation of airway epithelial cell activities related to eosinophil inflammation. J Asthma. 2010; 47(4): 381-387. doi: 10.3109/02770901003759410
  14. Fujisawa T, Kato Y, Atsuta J, Terada A, Iguchi K, Kamiya H, Yamada H, Nakajima T, Miyamasu M, Hirai K. Chemokine production by the BEAS-2B human bronchial epithelial cells: differential regulation of eotaxin, IL-8, and RANTES by TH2- and TH1-derived cytokines. J Allergy Clin Immunol. 2000; 105(1 Pt 1): 126-133. doi: 10.1016/s0091-6749(00)90187-8
  15. Meyer-Hoffert U, Lezcano-Meza D, Bartels J, Montes-Vizuet AR, Schroder JM, Teran LM. Th2-and to a lesser extent Th1-type cytokines upregulate the production of both CXC (IL-8 and gro-alpha) and CC (RANTES, eotaxin, eotaxin-2, MCP-3 and MCP-4) chemokines in human airway epithelial cells. Int Arch Allergy Immunol. 2003; 131(4): 264-271. doi: 10.1159/000072138
  16. Kim KJ, Jeon WY, Lee MY, Seo CS, Li HS, Shin HK. Inhibitory activity of Socheongryong‑tang and its constituent components against the production of RANTES, eotaxin, eotaxin-3 and MMP-9 from BEAS-2B cells. Mol Med Rep. 2014; 10(6): 3035-3046. doi: 10.3892/mmr.2014.2637
  17. Stellato C, Beck LA, Gorgone GA, Proud D, Schall TJ, Ono SJ, Lichtenstein LM, Schleimer RP. Expression of the chemokine RANTES by a human bronchial epithelial cell line. Modulation by cytokines and glucocorticoids. J Immunol. 1995; 155(1): 410-418. https://doi.org/10.4049/jimmunol.155.1.410
  18. Shin SH, Heo WW, Ye MK, Park JY, Cho CH, Sohn JH, Lee SH. Expression of eotaxin and RANTES mRNA in Nasal Polyps and its effect on eosinophils. Korean J Otorhinolaryngol-Head Neck Surg. 2000; 43(7): 724-730.
  19. Shin SH, Park JY, Jeon CH, Choi JK, Lee SH. Quantitative analysis of eotaxin and RANTES messenger RNA in nasal polyps: Association of tissue and nasal eosinophils. Laryngoscope. 2000; 110: 1353-1357. doi: 10.1097/00005537-200008000-00025
  20. Lim HS, Ha H, Seo CS, Jin SE, Kin Y, Jeon WY, Yoo SR, Shin IS, Kim JH, Kim SS, Shin NR, Lee MY, Jeong SJ, Kim OS, Shin HK. Comparative study on efficacies and ingredient contents of different solvent extracts of Insampaedok-san. Kor J Herbol. 2012; 27(6): 115-122. doi: 10.6116/kjh.2012.27.6.115
  21. Jo IJ. Inhibitory activity of Terminalia chebula extract against TNF-α/IFN-γ-induced chemokine increase on huma keratinocyte, HaCaT cells. Kor J Herbol. 2022; 37(3): 41-47. https://doi.org/10.6116/kjh.2022.37.3.41
  22. Wu SJ, Huang WC, Cheng CY, Wang MC, Cheng SC, Liou CJ. Fisetin suppresses the inflammatory response and oxidative stress in bronchial epithelial cells. Nutrients. 2022; 14(9): 1841. https://doi.org/10.3390/nu14091841