• Journal of Sericultural and Entomological Science
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• v.35 no.2
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• pp.129-133
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• 1993

The 44Md plasmid of Bacillus thruingiensis var. kurstaki HD-1(B. t k HD-1) was partially digested with Sau3AI and the fragments were cloned into E. coli HB101 on vector pBR322. Of 2, 950 clones with a recombinant pBR322, only one clone KC1 was determined to have the gene for crystal toxic proteins from the 44Md plasmid of B. t k HD-1 at the BamHI site of pBR322. The recombinant pBR322 was named pKC1 and its molecular size was 12kb. The KC1 produced a protein which was toxic to the silkworm and antigenically similar to the crystal toxic protein of B. t k HD-1. Also, electrophoretic mobility of the KC1 protein was apparently the same as that of the crystal toxic protein of B. t k HD-1.

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.2
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• pp.290-296
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• 2002

Serum immunoglobulins (Igs) from Israeli carp were purified using affinity chromatography. Fish were immunized with purified mouse IgG, and the specific fish antibodies were purified from the immune serum on a mouse IgG-immobilized agarose gel. Rabbit anti-Israeli carp Igs (R $\alpha$ I. carp Igs) antibodies were produced following hyperimmunization with mouse IgG specific carp antibodies. SDS-PAGE analysis under reducing condition showed that Israeli carp Igs were composed of two $\mu$-like heavy chains with about 82 and 50 kd, respectively, and one light chain with about 25 kd. On immunoblotting analysis, however, R $\alpha$ I. carp Igs failed to react with the light chain. When both protein A and protein G-purified normal carp Ig were compared with mouse IgG-specific Israeli carp Ig, no significant structural differences among them were observed. To investigate if there is any homology between other fish Ig molecules, cross-reactivity of R $\alpha$ I. carp Igs against Ig molecules from 6 different fish sera and mouse control serum was checked on immunoblotting analysis. As a result, R $\alpha$ I. carp Igs responded to Israeli carp, common carp, and tilapia Ig molecules. In flow cytometry study, however, R $\alpha$ I. carp Igs appeared to recognize 42.0%, 35.8% and <5% of Israeli carp, common carp and tilapia $Ig^+$ head kidney cells, respectively. The result suggests the heterogeneity between receptor Igs on B-like lymphocytes and soluble Igs in serum. It is crucial to obtain pure fish Igs to produce reagent antibodies as tools for the study on their specific immune responses.

• Proceedings of the Korea Water Resources Association Conference
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• 2011.05a
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• pp.238-238
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• 2011

복단면 하도는 홍수소통을 위한 상부 하도와 생태계 서식처 강화와 유사이송능력 개선, 경제적인 유지유량의 확보 등을 도모하는 자연적인 저수로 하도로 구성된다. 그러므로 갈수기와 홍수기의 유량차가 큰 하천에서, 복단면 하도는 하천 공학, 환경 생태 관점에서 다양한 이점을 제공한다. 그러나 복단면 하도에서의 흐름저항, 조도계수, 통수능, 수위-유량관계, 횡방향 유속분포, 그리고 하상전단응력 분포는 단단면 하도와 상이하며, 중요한 차이점은 주수로부와 홍수터부 사이의 유속차로 인하여 발생하는 활발한 운동량 교환에 의해 추가적인 전단층이 생성되는 점이다. 주수로와 홍수터 사이에서 발생된 경계전단력(Apparent Shear Force, ASF)은 하천의 전반적인 통수능을 감소시켜 홍수의 하도내 저류효과를 증가시킬 수도 있다. 또한 주수로 및 홍수터가 분담할 수 있는 유량은 경계전단력에 민감하기 때문에, 홍수터에 수목 식재, 구조물 설치 등을 계획할 경우 정량적인 항력저항의 산정을 위하여 매우 중요하다. 현재까지 복단면에서 발생하는 경계전단응력을 추정하는 다양한 방법들이 개발되었으며, 각각의 방법으로 부터 경계전단력을 정량적으로 예측할 수 있다. 그러나 대부분의 방법이 실험에 근거한 경험적 방법이므로 각 식의 개발에 사용된 자료에만 적합할 수 있는 한계가 있으며, 균일한 수로의 기하학적 변수(전단면과 주수로의 하폭비, 홍수터와 주수로의 수심비, 홍수터와 주수로의 조도비, 주수로의 하폭대 수심비 등)에 의한 식으로 구성되어 수치모형에서 직접 활용하기에는 한계가 있다. 따라서 본 연구에서는 복단면 경계전단력 산정을 위하여 개발된 다양한 연구결과들을 비교 검토하고, 기존문헌 또는 웹상에서 가용한 수리실험 자료들을 이용하여 각 방법에서 계산된 유량과 실측 유량을 비교함으로써 각 방법이 지닌 한계를 분석하였다. 본 연구에서 검토된 5가지 방법은 Knight and Demetriou (1983)(이하 KD), Wormleaton and Merret (1990)(이하 WM), Cristodoulou (1992), Bousmar and Zech(1999) (이하 BZ) 그리고 Moreta and Martin-Vide (2010)(이하 MM)이다. BZ 방법을 제외하고 나머지 방법들은 전체 유량의 비교에서 5% 이내의 오차를 나타내었다. 그러나 나머지 4가지 방법 중 주수로부 유속의 비교에 있어서는 소규모 수로 실험자료만 이용한 KD 방법이 5% 이상의 오차를 나타내어 정확도가 떨어지는 것으로 나타났다. 따라서 비교적 단순한 형태의 Cristodoulou (1992) 방법이 적용하고자 하는 수로의 규모와 무관하게 복단면 유량예측에 적합할 것으로 판단된다.

• The KIPS Transactions:PartD
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• v.13D no.4 s.107
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• pp.477-490
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• 2006

Existing index structures need very much update cost because they repeat delete and insert operations in order to update continuously moving objects. In this paper, we propose a new index structure which reduces the update cost of continuously moving objects. The proposed index structure consists of a space partitioning index structure that stores the location of the moving objects and an auxiliary index structure that directly accesses to their current positions. In order to increase the fanout of the node, it stores not the real partitioning area but kd-tree as the information about the child node of the node. In addition, we don't traverse a whole index structure, but access the leaf nodes directly and accomplish a bottom-up update strategy for efficiently updating the positions of moving objects. We show through the various experiments that our index structure outperforms the existing index structures in terms of insertion, update and retrieval.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.13 no.1
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• pp.439-444
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• 2012

This study was conducted to determine the effects of electrolytic water washing(EWW) and tap water washing(TWW) on proximate composition, color difference and SDS-PAGE changes of Mackerel(Scomber japonicus) muscle. Moisture contents of washed mackerel sediments EWW were much higher than TWW(p<0.05). Crude proteins of washed mackerel sediments EWW were 1% lower than TWW. Crude lipides had same results with crude proteins. Hunter value L, a, b were tested to each samples. $L^*$ values of TWW were higher than EWW. Both of $aL^*$ values were lower with washing times in order of 3rd>2nd>1st(p<0.05) but 2nd and 3rd of EWW were not significantly different(p>0.05). $b^*$ values were not different between the TWW and EWW(p<0.05). SDS-PAGE patterns of EWW muscle sediments were more darkeness 205KD band than TWW muscle sediments. In these results said that EWW is better than TWW for red meat kamaboko industry, respectively.

• Microbiology and Biotechnology Letters
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• v.22 no.5
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• pp.507-514
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• 1994

Acetyl xylan esterase was produced by E. coli HB101 harboring a recombinant plasmid pKMG6 which contained the estI gene of Bacillus stearothermophilus. The maximum production was observed when the E. coli strain was grown at 37$\circC for 12 hours in the medium containing 0.5% acetyl xylan, 1.0% tryptons, 1.0% sodium chloride, and 0.5% yeast extract. The esterase produced was purified to homogeneity using a combination of ammonium sulfate fractionation, DEAE Sepharose CL-6B ion exchange chromatography and Sephacryl S-200 gel filtration. The native enzyme had an apparent molecular mass of 60 kd and was composed of two identical subunits of 29 kd. The N-terminal amino acid sequence of the polypeptide was Ala-X-Leu-Gln- Ile-Gln-Phe-X-X-Gln. The acetyl esterase displayed a pH optimum of 6.5 and a temperature opti- mum of 45$\circC. The heavy metal ions such as Ag$^{++}$, Hg$^{++}$ and Cu$^{++}$ inhibited nearly completely the activity of the esterase, and no specific metal ion was found to be required for the enzyme activity. The enzyme readily cleaved MAS, $\beta$-D-glucose pentaacetate, $\alpha$-naphthyl acetate, $\rho$-nitrophenyl acetate as well as acetyl xylan, but had no activity on $\rho$-nitrophenyl propionate, $\beta$-nitrophenyl butyrate or $\beta$-nitrophenyl valerate. The Km and Vmax values for MAS were 2.87 mM and 11.55 $\mu$mole/min, respectively. Synergistic behavior was demonstrated with a combination of xylanase and esterase from B. stearothermophilus in hydrolyzing acetyl xylan.

• Applied Microscopy
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• v.24 no.2
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• pp.12-25
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• 1994

This research was undertaken to determine the effects of the anticancer and immunosuppressive drug cyclophosphamide (CP) on the epididymis of the male rat in terms of ultrastructural alteration and protein analysis by SDS-PAGE at different groups; control group, 1 week group, 3 weeks group, 5 weeks group were treated with saline (control group) or CP at doses of 20mg/Kg/week, 1 time a week, respectively. In the cytoplasm of the principal cells on the epididymis, the mitochondrial outer and inner membranes were significantly swollen or disrupted. The cisterns of rough endoplasmic reticulum (rER) were also swollen, and a number of Golgi vesicles were increased, respectively. It is suggested that treatment with CP alters the specific cell organelles in all segments of the epididymis. CP caused changes in protein concentrations in cauda of epididymis after CP treatment. Total proteins of 30 to 39 species such as lactate dehydrogenase, carnitine acetyltransferase and acid phosphatase were expressed in the cauda fluid. Then the more CP was increased, the more concentration of proteins caused to decrease, synthesize or increase in epididymal cauda. In contrast to the control group, in particular 29KD and the other 10 proteins in the cauda fluid were decreased or disappeared, respectively, whereas 89KD and the other 6 proteins in the cauda, were increased or synthesized, respectively. The other proteins are not showed distinctive difference. Therefore, it is possible that CP at a high dose accumulation alters epididymal function with dose-related increase or decrease in specific activity of marked proteins for all regions of the epididymis (particularly, specific segment of cauda). These alterations could be mediated by direct, toxic effects of the drug on the epithelium or be secondary to changes in the spermatozoa as a result of the CP treatment.

• Journal of Veterinary Clinics
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• v.7 no.1
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• pp.391-402
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• 1990

To examine the effects of vaccination against Babesia gibsoni infection in dogs, 15 normal mixed-breed dogs(5 month to 1 year old) divided into 3 groups with 5 dogs in a group. One of them was selected as control group(group A) and others were selected as experimental groups(group B and C). The group B was vaccinated with sonicated antigens and the group C was vaccinated with 0.2% of formalin treated antigens. The results obtained in the examination were summarized as follows : 1. In the western blot, the lane A revealed specific two bands on the regions of 54kd and 100kd, respectively. 2. After the first vaccination, the antibody titers of group B and C were higher 5 times(1 ： 200) than those of control group(1：40). After the second vaccination, the antibody titers of group B and C have not changed. When challenged with the protozoa(Babesia gibsoni), the antibody titers(1 : 5,000) were elevated in all groups. But these were not exceeded over 1 ： 5,000 for 4 weeks. 3. After challenge, the peak time of increased numbers of the protozoa was the 15th day (12-18 days) in all groups. During these days, the rate of parasitized erythrocytes in control group was 55.0${\pm}$5.4%. But those of group B and group C were 26.0${\pm}$6.4%, and 15.6${\pm}$7.8％, respectively. 4. After challenge, all of the values of PCV, Hb, RBC were shown to decrease in all of the control and experimental groups. 5. The total leukocytes counts are shown a tendency of reduction in all groups after challenge. 6. In all groups, there were increase in lymphocytes and monocytes after challenge.

• Journal of Sericultural and Entomological Science
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• v.36 no.1
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• pp.30-36
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• 1994

choChorion(egg-shell) morphology of the F1 hybrid between Bombyx mori and Bombyx mandarina has been observed by scanning electrom microscope and chorion protein was analyzed by electrophoresis. The chorion surface structure of F1 hybrids in the lateral(flat) region was similar to that of maternal line. The F1 hybrids chorion was found to have basically a three layer structure. The middle and inner layer were very much like those of the Bombyx mandarina and Bombyx mori. There were many conic pillar structures in the outer layer of the F1 hybrid, which was similar to Bombyx mandarina. This conic pillar structure had a thin cover layer was more clear in the dorsal and ventral side of the F1 hybrid chorion. The conic pillar structure of Bombyx mandarina was found to be dominant in F1 hybrid chorion irrespective of their maternal line. Major components of chorion protein were analyzed by two-dimensional electrophoresis and found to have isoelectric points in the range of pH 4.0-6.5 and molecular weight 10 to 50 kd. F1 hybrid chorion protein components related directly to those of the maternal line. The conic pillar structure was dominat characteristic and it was present in all F1 hybrid.

• Korean Journal of Veterinary Research
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• v.52 no.3
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• pp.199-203
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• 2012

Neurofascin, one of the members of L1CAM, has been known to have some important roles during the development of nerve fibers. In order to investigate the role of neurofascin associated with the development of nerve fibers in the rat sciatic nerve, the initial development of NF155 in the paranode was studied with immuno-fluorescence and immuno-electron microscopy. The result of the present study showed NF155 was not detected in the fetal sciatic nerve and began to reveal at the postnatal day 0 (P0) and dramatically increased by time lapse until postnatal day 7 (P7). NF155 was prominently localized in the axolemma of paranode and not detected in the central region of node of Ranvier. According to the present study, NF155 is likely to have some relationships with the formation of paranode and myelin sheath.