Morphological and Biochemical Characterization of the Chorion in Interspecific Hybrid Between Bombyx mori and Bombyx mandarina

집누에(Bombyx mori)와 멧누에(Bombyx mandayina)의 종간교잡에 있어서 란각구조 및 Chorion 단백질

choChorion(egg-shell) morphology of the F1 hybrid between Bombyx mori and Bombyx mandarina has been observed by scanning electrom microscope and chorion protein was analyzed by electrophoresis. The chorion surface structure of F1 hybrids in the lateral(flat) region was similar to that of maternal line. The F1 hybrids chorion was found to have basically a three layer structure. The middle and inner layer were very much like those of the Bombyx mandarina and Bombyx mori. There were many conic pillar structures in the outer layer of the F1 hybrid, which was similar to Bombyx mandarina. This conic pillar structure had a thin cover layer was more clear in the dorsal and ventral side of the F1 hybrid chorion. The conic pillar structure of Bombyx mandarina was found to be dominant in F1 hybrid chorion irrespective of their maternal line. Major components of chorion protein were analyzed by two-dimensional electrophoresis and found to have isoelectric points in the range of pH 4.0-6.5 and molecular weight 10 to 50 kd. F1 hybrid chorion protein components related directly to those of the maternal line. The conic pillar structure was dominat characteristic and it was present in all F1 hybrid.

집누에와 멧누에의 종간교잡에 의한 란각구조 및 chorion 단백질의 변이와 양 종간의 유연관계를 검토했다. 1. 교잡종 란의 측면부의 란각표면구조는 양 종이 모두 집누에와 유사한 그물상구조를 나타내지만 각각의 교잡 모체쪽의 구조와 유사했다. 2. 교잡종에서의 란각단면구조는 멧누에의 구조와 유사했으며 최외층인 덮개층은 측면부에서 주변부로 갈수록 더욱 뚜렷이 관찰되었다. 3. Chorion 단백질의 성분분석 결과 등전점이 4.0-6.5 분자량 10-50 kd 사이에 대부분의 성분이 검출되었으며 교잡종이 chorion 단백질성분은 영동 pattern, 성분수, 등전점 및 분자량 등에 있어서 교잡모체의 chorion 단백질과 유사했다.