• 미생물학회지
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• 제25권1호
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• pp.46-51
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• 1987

In order to increase the production of tryptophan by maximizing expression of recombinant trp plasmid, Klebsiella pneumoniae KC 105(pheA tyrA trpE trpR tyrR) was genetically modified. KC 107, inosine monophospate(IMP) auxotroph from KC 105 and KC 108, histidine(His) auxotroph from KC 107 were also derived respectively to increase phosphoribosylpyrophosphate(PRPP) production which is required for tryptophan biosynthesis. From KC 107 phosphoribosylpyrophosphate consumption which is required for tryptophan biosynthesis. From KC 107 and KC 108, KC 109 and KC 110, both arginine auxotrophs were derived respectively. To investigate the expression of recombinant trp plasmid in the selected K. pneumoniae mutants, the auxotrophic mutants were transformed with recombinant trp plasmids pSC 101-$trpE^{FBR}$, pSC 101-trpL(.DELTA.att) $trpE^{FBR}$ (pSC 101-trp-AF). Amount of tryptophan produced and activities of tryptophan synthase of $trpR^{-}$ mutant (KC 100) and $tyrR^{-}$ mutnat(KC 105) containing recombinant plasmid pSC 101-trp operon were increased by 30-40% as compared with KC 99(pheA tyrA trpE) containing recombinant plasmid pSC 101-trp operon. Activities of tryptophan synthase and production of tryptophan of KC 108 ($His^{-}$) and KC 109($Arg^{-}$) containing recombinant plasmid pSC 101-trp operon were increase by two-fold as compared with KC 107 containing pSC 101-trp operon.

• 대한한의학회지
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• 제24권1호
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• pp.1-8
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• 2003

Purpose : Nowadays, there are many studies of growth retardation treatment by oriental medical therapy. This study was performed to evaluate the effect of Hwalhyulsungjang-san (Huoxuechengzhang-san) and KC101 on physical development in growth, Methods : 60 young (3 weeks old) male rats, whose mean weight was $46.8{\pm}0,7g$, were divided into 5 groups : groups A, B, C, and D were fed an experimental diet containing respectively Hwalhyulsungjang-san 2.5%, Hwalhyulsungjang-san 7.5%, KC101 5.0%, and KC101 10.0%, and the other group were fed an AIN-diet containing neither Hwalhyulsungjang-san nor KC101 as a control. Study of each group used 2 rats and was repeated in 6 times. Body weight, daily weight gain, dietary intake, body length, femur length, backbone length and serum concentration of IGF-I and hemoglobin were measured. Result : 1. Body weight and daily weight gain of group D (KC101 10%) were highest; dietary intake of group D was also increased by 13% over the control group. 2. Average body length of group D was increased to 1.69cm, which was 3.2% over the control group. Backbone length of group D was also increased 4% compared to control group. 3. Serum concentration of IGF-I of group D increased 17% compared to control group. 4. Serum concentration of hemoglobin of group B (Hwalhyulsungjang-san 7.5%) increased 8% compared to control group. Conclusion : This study showed that Hwalhyulsungjang-san and KC101 composed of oriental medicinal stuffs have effect on physical development in growing rats. There were not any statistical meaning compared to control group, but this study suggests that intake of these compounds from a young age may lead to increase physical development in growth.

• 한국잠사곤충학회지
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• 제35권2호
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• pp.129-133
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• 1993

Bacillus thruingiensis var. kurstaki HD-1의 내독소 단백질 유전자의 발현기작을 규명하기 위하여 이 균으로부터 내독소 단백질 유전자가 존재하는 것으로 확인된 29Md와 44Md plasmid를 분리한 후, Sau3AI 제어효소로 부분절단하고, pBR322 BamHI site에 ligation하여, E. coli HB101 strain에 transformation시켜, 3,000여개의 Ampr/Ters한 colony를 얻어 면역학적 방법과 살충적 검정으로 통해 재조합 균주 KC1을 얻었다. PKC1 plasmid DNA는 vector DNA를 포함하여 약 12kb 정도의 크기를 가지며, B. t k HD-1 내독소 단백질과 이동도가 같거나 일치하는 132kd, 117kd의 KC1 specific band 2개를 얻었다. KC1 cell extract를 첨식한 결과 약 80% 치사율을 보였다.

• 고려인삼학회:학술대회논문집
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• 고려인삼학회 1998년도 Advances in Ginseng Research - Proceedings of the 7th International Symposium on Ginseng -
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• pp.96-102
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• 1998

A lot of individual ginseng plants were selected in the farmer's fields to develop new ginseng varieties with desirable traits. Among them, a promising line has been developed through comparative cultivation of several lines selected with pure line separation of local raced. It was then designated as "KC (denotes Korean Ginseng)" and tested in the regional yield and adaptation trials for 10 years. KG lines grew vigorously after 4 years of age. Especially, KG 102 line arson운 them showed traits of multiple and short stems. As for the root characters, the length of taproot of KG 101 line was longer than that of local race, Jakyung-jong, or other lines ailed the root weight of KG 102 line waIn 15 % higher than that of local race. In general, KG 101, KG 103, KG 104, and Hwangsauk-jong had good root shape. Total amount of ginsenosides of ginseng taproot was the highest in KG 103 line than in local race and other lines. In these studies, we, elected three superior lines, KG 101, KG 102, and KG 103, having characteristic of good root shape, high yield, and large amount of ginsenoside, respectively.

• 대한본초학회지
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• 제28권6호
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• pp.101-109
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• 2013

Objectives : The purpose of this study was to investigate the effects of Polygoni Multiflori Radix Water Extract (PM) on the production of inflammatory mediators in RAW 264.7 mouse macrophages induced by lipopolysaccharide (LPS). Method : We examined effect of PM Extract on the cell viability of RAW 264.7 cells. Futhermore, we investigated anti-inflammatory effect of PM Extract by the production of proinflammatory cytokines such as NO, intracellular calcium, interleukin(IL)-$1{\alpha}$, IL-3, IL-$1{\beta}$, IL-6, interferon inducible protein-10(IP-10), keratinocyte-derived chemokine(KC) and vascular endothelial growth factor(VEGF). Result : No significant changes have been found in the mouse macrophge cell viability by the PM Extract at the concentration of 25, 50, 100 and $200{\mu}g/mL$. The water extract of PM significantly inhibited the production of NO and intracellular calcium in the LPS-induced macrophages at the concentration of 25, 50, 100 and $200{\mu}g/mL$. The water extract of PM significantly inhibited the production of IL-$1{\alpha}$, IL-${\beta}$, IL-3, IP-10, KC, VEGF in the LPS-induced macrophages at the concentration of 50, 100, and $200{\mu}g/mL$; IL-6 at the concentration of 100 and $200{\mu}g/mL$ ; and IL-17 at $200{\mu}g/mL$. Conclusion : The water extract of PM significantly inhibited the production of NO, intracellular calcium, IL-$1{\alpha}$, IL-3, IL-${\beta}$, IP-10, KC, VEGF at the concentration of 50 ㎍/mL or higher in the LPS-induced macrophages with no changes in the cell viability of them. These results suggest that water extract of Polygoni Multiflori Radix has anti-inflammatory effect regulating the production of proinflammatory cytokines in the LPS-induced macrophages.

• 생명과학회지
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• 제23권1호
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• pp.24-30
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• 2013

본 연구는 mtDNA cytochrome b (Cyt b) 유전자 서열을 토대로 한국흑우의 유전적 다양성 및 계통유전학적 위치를 파악하기 위하여 실시하였다. 한국흑우 38두로부터 결정된 mtDNA Cyt b 유전자 전체서열 내에서 염기삽입 및 결실 없이 1개의 silent mutation이 동정되었다. 또한 2개의 haplotype으로 분류되었고, 염기변이율 및 haplotype 다양성지수에 기초한 한국흑우의 유전적 다양성은 기존에 보고된 중국 품종에 비해 낮게 나타났다. 한국, 일본, 중국에 분포하고 있는 12품종 101개 서열을 수집하여 한국흑우와의 유연관계를 확인하였다. 한국흑우에서 분류된 2개의 haplotype은 모두 B. taurus 계열에 포함되었으며, 한우, 일본흑우, Yanbian, Zaosheng 등 4개 품종과 하나의 그룹을 형성하였다. 또한 품종별 Dxy 유전거리 산출 결과, 한국흑우는 한우 및 일본흑우에 비해서 중국의 Yanbian, Zaosheng 품종과 더 가까운 유연관계를 보였다. 본 연구의 결과는 가축유전자원으로서 한국흑우의 보존 및 유전적 특성 구명을 위한 중요한 자료로 활용이 가능할 것으로 사료된다.

• Journal of Microbiology and Biotechnology
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• 제28권7호
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• pp.1133-1140
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• 2018

Pseudomonas fluorescens KLR101 was found to be capable of producing polyhydroxyalkanoate (PHA) using various sugars and fatty acids with carbon numbers ranging from 2 to 6. The PHA granules consisted mainly of a poly(3-hydroxybutyrate) homopolymer and/or poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymer. Genomic DNA of P. fluorescens was fractionated and cloned into a lambda library, in which a 5.8-kb fragment that hybridized to a heterologous phaC probe from Ralstonia eutropha was identified. In vivo expression in Klebsiella aerogenes KC2671 (pUMS), restriction mapping, Southern hybridization experiments, and sequencing data revealed that PHA biosynthesis by P. fluorescens relied upon a polypeptide encoded by a 1,683-bp non-operonal ORF, which was preceded by a possible -24/-12 promoter and highly similar to DNA sequences of a gene encoding PHA synthase in the genus Pseudomonas. In vivo expression of the putative PHA synthase gene ($phaC_{Pf}$) in a recombinant Escherichia coli strain was investigated by using glucose and decanoate as substrates. E. coli (${phaC_{Pf}}^+$, pUMS) grown in medium containing glucose accumulated PHA granules consisting mainly of 3-hydroxybutyrate, whereas only a trace amount of 3-hydroxydecanoate was detected from an E. coli fadR mutant (${phaC_{Pf}}^+$) grown in medium containing decanoate. In vitro enzymatic assessment experiments showed that 3-hydroxybutyryl-CoA was efficiently used as a substrate of purified $PhaC_{Pf}$, suggesting that the putative PHA synthase of P. fluorescens utilizes mainly short-chain-length PHA precursors as a substrate.

• 미생물학회지
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• 제30권2호
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• pp.88-95
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• 1992

유전공학 기법으로 번형시킨 Km' plasmid 들의 전이를 30.deg.C 의 Luris-Bertani broth 에서 실시하였으며, 그 전이 빈도에 대한 donor 와 recipient 의 균주와 함께 pH 의 영향을 연구하였다. GEM 균주들과 NI 균주의 Km'plasmid 의 전이빈도는 recipient 가 MTI 일 경우 pH 7 에서 약 $10^{-5}$ 로 비슷하였으나 CS 균주인 DKC601 에서는 Km'plasmid 의 전이빈도가 $2.2 * 10^{-7}$ 로 훨씬 떨어졌다. 그리고 recipient 가 lab. strain (E. coli HB101) 인 경우에도 Km'plasmid 의 전이경향은 GEM 균주들과 NI 균주사이에 큰차이가 없었다. 또 어느 균주의 경우에나 pH 7 일 때의 전이 빈도가 $10^{-5}$ 정도로 가장 높게 나타났으며, pH 5 와 pH 9 에서는 그보다 조금 떨어졌다. 그러나 E. coli C600을 host 로 하여 제조한 CS 균주(DKC601)에서는 Km'plasmid 의 전이 빈도가 다른 GEM 균주들에 비하여 $10^{2}$ $-10^{3}$ 배 낮았으며 pH 4와 9 에서 6 시간의 conjugation 후에도 전이가 전혀 일어나지 않았다. Conjugant 에서의 plasmid의 재배열은 lab, strain 을 recipient 로 했을 경우에는 별로 없었지만, NI 균주를 recipient 로 했을 경우에 많이 나타났으며, 특히 donor 가 NI 균주보다 GEM 균주인 경우에 더욱 다양하였다. 그러나 pH 에 따른 타이는 그렇게 크지 않았다.

• 대한약리학회지
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• 제24권1호
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• pp.93-101
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• 1988

돼지 우관상동백을 적출하여 항경련제인 oxybutynin의 약리작용을 조사하였다. 1. Acetylcholine (ACh)과 KCl은 관상동맥을 수축시켰고 이 수축효과는 용량의존적이었다. ACh의 수축효과는 내피손상표본 $(EC_{50}=0.52\;{\mu}M)$에서 내피표본 $(EC_{50}=0.52\;{\mu}M)$보다 약 2배 강화되있으나 KCl의 수축효과는 양군간에 차이가 없었다. 2. $ACh(1.0\;{\mu}M)$의 수축효과는 oxybutynin과 atropine에 의 하여 용량의존적으로 억제되었고 두 약물의 $(IC_{50}$는 각각 11.0 nM과 0.47 nM로 atropine이 약 23배나 더 예민하였다. 그러나 KCl (35 mM)의 수축효과는 atropine으로는 전혀 영향받지 않았고 oxybutynin으로는 용량의존적으로 억제 되었으며 $(IC_{50}=49.7\;{\mu}M)$이였다. 3. ACh의 용량반응곡선은 oxybutynin $(IC_{50}=11\;nM)$ 및 atropine $(IC_{50}=0.47\;nM)$ 전처 리 하에서 우측으로 평 행 이 동되 있고, KCI의 용량반응곡선은 oxybutynin $(IC_{50}=49.7\;nM)$ 전처리하에서 우측으로 비상경적 이동을 일으켰다. 4. Oxybutynin은 Bay K 8644 $(0.1\;{\mu}M)$의 수축효과를 용량의존적 으로 억제하였고 $(IC_{50}=63.0\;{\mu}M)$이었으며 , histamine $(35\;{\mu}M)$의 수축효과는 oxybutynin의 최대량 $(500\;{\mu}M)$으로 부분억제 (최대 50%)만을 일으켰다. 이상의 성적으로 적출돼지 관상동백에서 내피세포는 ACh에 의한 수축반응에 억제적 영향을 미치며, oxybutynin은 강력한 muscarine receptor 차단작용과 calcium influx 억제작용에 의하여 혈관근 이완을 일으킨다고 추론하였다.