• 공업화학
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• 제20권4호
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• pp.449-452
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• 2009

철,셀레늄,그리고 몰리브데늄이 결여되어 있는 최소배지는 Escherichia coli MC4100의 혐기성 배양에서의 수소생산을 억제하였다. 철, 셀레늄, 그리고 몰리브데늄은 대장균과 Enterobacter 모두의 formate dehydrogenase ($FDH_{II}$) 효소의 보조인자로 알려져 있다.그러므로 이러한 미량성분들이 최소배지에 결여되어 있을 때는 $FDH_{II}$를 통한 수소생산이 대장균 뿐만 아니라 E. aerogenes에서도 저해될 것이다. 이러한 성분들이 부족할 때 E. aerogenes 413에 의한 수소 생산은 지연되었다.그러므로,E. aerogenes에 의한 수소 생산은 NADH의 재산화가 아닌 포메이트 탈카복시 반응에 의해서 시작된다고 사료된다.

• Biomolecules & Therapeutics
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• 제3권4호
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• pp.311-315
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• 1995

The K. aerogenes ureal gene product was previously shown to facilitate assembly of the crease metallocenter (Lee, M. H., Mulrooney, S. B., Renner, M. J., Markowicz, Y., and Hausinger, R. P. (1992) J. Bacteriol. 174, 4324-4330). UreG protein has now been purified and characterized. Although the protein is predicted to possess a putative NTP-binding P-loop motif, equilibrium dialysis studies showed negative results. Immunogold electron microscopic studies using polyclonal antibodies directed against UreG protein confirm that UreG is located in the cytoplasm as predicted in the DNA sequence.

• Journal of Korean Neurosurgical Society
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• 제29권1호
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• pp.126-130
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• 2000

A 40-year-old female with hypertensive thalamic hemorrhage, secondary intraventricular hemorrhage, and hydrocephalus was treated with extraventricular drainage. She developed catheter-related ventriculitis caused by gram-negative rods, Enterobacter aerogenes. She was treated with systemic pefloxacine, ceftazidime, amikin and intraventricular vancomycin, gentamicin was unsuccessful. The ventriculitis was successfully controlled by intraventricular administration of the pefloxacine. Regarding their excellent activity against gram-negative rods, Enterobacter aerogenes, and probable safety when administered intraventricularly, administration of the pefloxacine, may be considered in the treatment of ventriculitis if the pathogen is resistant to other conventional antibiotics.

• 한국미생물·생명공학회지
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• 제15권2호
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• pp.112-115
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• 1987

B. megaterium ATCC 13639와 E. aerogenges의 비타민 B$_{12}$ 생산을 위한 최적 pH는 각각 6.0과 5.0으로 pH의 영향을 크게 받았으나 두 세균에 의한 riboflavin 생산은 pH에 따른 변화가 거의 없었다. Sucrose를 첨가하면 두 비타민 생산량이 크게 증가되었고 최적pH도 변함을 알 순 있었다. 두 세균에 의한 두 비타민 생산에 미치는 온도의 영향은 pH에 비하여 아주 적었다. 이상의 결론은 장차 여러 가지 식품 폐기물을 이용한 비타민 B$_{12}$와 riboflavin의 생산이 가능함을 보여주었다.

• KSBB Journal
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• 제23권1호
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• pp.54-58
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• 2008

수소는 연료전지와 같은 친환경적인 용도로 인해 미래의 에너지로서 주목받고 있는데, 생물학적인 발효법은 수소의 생산을 위한 유망한 방법이다. 본 연구에서는 Enterobacter aerogenes KCCM 40146을 대상으로 수소 생산을 최대로 하기 위한 배지의 조건을 최적화하였다. 그 결과, 0.5M potassium phosphate buffer pH 6.5에서 glucose 30 g/L일 때 수소의 누적 농도가 431 $m{\ell}$로 최대값을 얻을 수 있었다. 질소원으로 peptone과 tryptone을 넣은 배지가 수소의 생산 뿐 아니라 균주의 성장에 가장 효율적이었다 한편, 미생물의 성장속도조절이 수소의 효율적 생산을 위해 중요한 실험변수임을 알 수 있었다.

• 농업과학연구
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• 제43권1호
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• pp.100-108
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• 2016

An auxin-producing bacteria (strain 5-1) was isolated from button mushroom compost in Boryeong-Si, Chungcheongnam-Do. The 5-1 strain was classified as a novel strain of Enterobacter aerogenes based on chemotaxonomic and phylogenetic analyses. The isolated E. aerogenes 5-1 was confirmed to produce indole-3-acetic acid (IAA), one of the auxin hormones, using TLC and HPLC analyses. When the concentration of IAA was assessed by performing HPLC quantitative analysis, a maximum concentration of IAA of $109.9mgL^{-1}$ was detected in the culture broth incubated in R2A medium containing 0.1% L-tryptophan for 24 h at $35^{\circ}C$. Acidification of the culture was deemed caused by an increase of IAA because a negative relationship between IAA production and pH was observed. Supplementation with a known precursor of IAA production, L-tryptophan, appeared to induce maximal production at 0.1% concentration, but it reduced production at concentrations above 0.2%. To investigate the growth-promoting effects to crops, the culture broth of E. aerogenes 5-1 was used to inoculate water cultures and seed pots of mung bean and lettuce. In consequence, adventitious root induction and root growth of mung bean and lettuce were two times higher than those of the control.

• KSBB Journal
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• 제26권3호
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• pp.223-228
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• 2011

The effects of pH, glycerol concentration and salt on cell growth and ethanol production using Enterobacter aerogenes KCTC 2190 were evaluated in the anaerobic culture condition. In condition of initial pH 5, cell growth and ethanol production were highest. An initial concentration of 10 g/L of pure glycerol gave the highest cell growth and ethanol production. However, in case of over 15 g/L of pure glycerol, they decreased. The cell growth and ethanol production decreased with the increase of salt concentration. When 10 g/L of crude glycerol was used as the carbon source, the cell growth and ethanol production were $1.32\;OD_{600}$ and 3.95 g/L, respectively, which were about 94.4% and 88.5% compared to those of pure glycerol. These result indicates that the crude glycerol produced in the biodiesel manufacturing process maybe useful as a potential carbon source for ethanol production form Enterobacter aerogenes KCTC 2190.

• Journal of Microbiology and Biotechnology
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• 제27권8호
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• pp.1491-1499
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• 2017

Trimethylamine N-oxide (TMAO), which is transformed from trimethylamine (TMA) through hepatic flavin-containing monooxygenases, can promote atherosclerosis. TMA is produced from dietary carnitine, phosphatidylcholine, and choline via the gut microbes. Previous works have shown that some small molecules, such as allicin, resveratrol, and 3,3-dimethyl-1-butanol, are used to reduce circulating TMAO levels. However, the use of bacteria as an effective therapy to reduce TMAO levels has not been reported. In the present study, 82 isolates were screened from healthy Chinese fecal samples on a basal salt medium supplemented with TMA as the sole carbon source. The isolates belonged to the family Enterobacteriaceae, particularly to genera Klebsiella, Escherichia, Cronobacter, and Enterobacter. Serum TMAO and cecal TMA levels were significantly decreased in choline-fed mice treated with Enterobacter aerogenes ZDY01 compared with those in choline-fed mice treated with phosphate-buffered saline. The proportions of Bacteroidales family S24-7 were significantly increased, whereas the proportions of Helicobacteraceae and Prevotellaceae were significantly decreased through the administration of E. aerogenes ZDY01. Results indicated that the use of probiotics to act directly on the TMA in the gut might be an alternative approach to reduce serum TMAO levels and to prevent the development of atherosclerosis and "fish odor syndrome" through the effect of TMA on the gut microbiota.

• 한국식품영양학회지
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• 제21권1호
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• pp.15-21
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• 2008

In this study, the minimum inhibition concentration(MIC), growth inhibition activity, and colony forming inhibitory activity of water soluble propolis against Bacillus cereus, Bacillus subtilis, Listeria monocytogenes, Staphylococcus aureus, Streptococcus mutans, Citrobacter freundii, Enterobacter aerogenes, Escherichia coli, Klebsiella pneumoniae and Salmonella enteritidis were tested. The MICs of the water soluble propolis against Bacillus cereus, Bacillus subtilis, Listeria monocytogenes, Staphylococcus aureus, Streptococcus mutans, Citrobacter freundii, Enterobacter aerogenes, Escherichia coli, Klebsiella pneumoniae, and Salmonella enteritidis were 312.5 ppm, below 156.3 ppm, 625 ppm, 10,000 ppm, above 10,000 ppm, 10,000 ppm, above 10,000 ppm, above 10,000 ppm, 10,000 ppm, and above 10,000 ppm, respectively. The growth inhibition concentrations against Bacillus cereus, Bacillus subtilis, Listeria monocytogenes, Staphylococcus aureus, Streptococcus mutans, Citrobacter freundii, Enterobacter aerogenes, Escherichia coli, and Klebsiella pneumoniae were 156.3 ppm, below 156.3 ppm, 625 ppm, 5,000 ppm, 10,000 ppm, 10,000 ppm, 10,000 ppm, 10,000 ppm, and 5,000 ppm, respectively. However, 10,000 ppm did not inhibit the growth of Salmonella enteritidis. Finally, the colony forming inhibitory activities against Bacillus cereus, Bacillus subtilis, Listeria monocytogenes, Staphylococcus aureus, Streptococcus mutans, Citrobacter freundii, Enterobacter aerogenes, Escherichia coli, Klebsiella pneumoniae, and Salmonella enteritidis were 98.0%, 99.8%, 69.8%, 98.1%, 62.0%, 63.1%, 79.5%, 61.9%, 79.6%, and 0.0%, respectively.

• 한국토양비료학회지
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• 제49권4호
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• pp.318-326
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• 2016

Two phosphate solubilizing bacteria, Pantoea rodasii PSB-11and Enterobacter aerogenes PSB-12, were isolated from button mushroom compost and employed to assess their synergistic effect in liquid medium and on growth of green gram plants by single and co-inoculation of the strains. Co-inoculation of two strains was found to release the highest content of soluble phosphorus ($521{\mu}g\;ml^{-1}$) into the medium, followed by single inoculation of Pantoea strain ($485{\mu}g\;ml^{-1}$) and Enterobacter strain ($470{\mu}g\;ml^{-1}$). However, there was no significant difference between single inoculation of bacterial strain and co-inoculation of two bacterial strains in terms of phosphorous release. The highest pH reduction, organic acid production and glucose consumption was observed in the E. aerogenes PSB-12 single inoculated culture medium rather than those of co-inoculation. According to the plant growth promotion bioassay, co-inoculated mung bean seedlings recorded 10.6% and 10.7% higher shoot and root growth respectively compared to the control. Therefore, in concluding, co-inoculation of the strains P. rodasii and E. aerogenes displayed better performance in stimulating plant growth than inoculation of each strain alone. However, being short assessment period of the present study, we recommend in engaging further works under field conditions in order to test the suitability of the strains to be used as bio-inoculants.