• Journal of Life Science
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• v.8 no.5
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• pp.604-613
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• 1998

The last enzyme of the sesquiterpen phytoalexin capsidiol synthesis in tobacco cell, 5-epi-aristolochene hydro-xylase which convert 5-epi-aristolochene (EAS) to capsidiol, was cloned by a reverse transcription polymerase chain reaction strategy and cDNA library screening. Cloned CYP-B3 contained high probability amino acid matches to known plant cytochrome P450 sequences and open reading frame with the conserved FxxGxRxCxG heme-binding region. Transcripts of CYP-B3 were not detected in control cells, but induced in elicitor-treated cells. Furthermore, CYP-B3 transcripts were induced by fungal extracts and cellulase but not by other stimuli(chilling, heat shock and 2,4-D). Induction of CYP-B3 transcripts by elicitor treatment was not affected by ancymidol and ketoconazole treat-ments suggesting that an inhibition of hydroxylase activity by Cyt P450 inhibitors resulting from post translational processing event.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.43 no.8
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• pp.748-756
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• 2015

In this paper, we developed a miniaturized real time video compression system satisfying the military environment using ADV212 and FPGA. We present an efficient hardware design scheme for the weight reduction of the device and also a software solution to deal with noisy image signals. Experimental results show that the frame delay is reduced by a factor of 2 or 3 and the device's weight is decreased by a factor of 6 to 7. In order to prove the reliability for the military usage of this development, we examine the environmental test (MIL-STD-810G) and EMI test (MIL-STD-461F). Experimental results show that the developed system satisfies the requirements.

• Journal of Communications and Networks
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• v.18 no.1
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• pp.65-74
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• 2016

Ship ad-hoc network (SANET) extends the coverage of the maritime communication among ships with the reduced cost. To fulfill the growing demands of real-time services, the SANET requires an efficient clock time synchronization algorithm which has not been carefully investigated under the ad-hoc maritime environment. This is mainly because the conventional algorithms only suggest to decrease the beacon collision probability that diminishes the clock drift among the units. However, the SANET is a very large-scale network in terms of geographic scope, e.g., with 100 km coverage. The key factor to affect the synchronization performance is the signal propagation delay, which has not being carefully considered in the existing algorithms. Therefore, it requires a robust multi-hop synchronization algorithm to support the communication among hundreds of the ships under the maritime environment. The proposed algorithm has to face and overcome several challenges, i.e., physical clock, e.g., coordinated universal time (UTC)/global positioning system (GPS) unavailable due to the atrocious weather, network link stability, and large propagation delay in the SANET. In this paper, we propose a logical clock synchronization algorithm with multi-hop function for the SANET, namely multi-hop clock synchronization for SANET (MCSS). It works in an ad-hoc manner in case of no UTC/GPS being available, and the multi-hop function makes sure the link stability of the network. For the proposed MCSS, the synchronization time reference nodes (STRNs) are efficiently selected by considering the propagation delay, and the beacon collision can be decreased by the combination of adaptive timing synchronization procedure (ATSP) with the proposed STRN selection procedure. Based on the simulation results, we finalize the multi-hop frame structure of the SANET by considering the clock synchronization, where the physical layer parameters are contrived to meet the requirements of target applications.

• Horticultural Science & Technology
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• v.34 no.1
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• pp.102-111
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• 2016

The goal of this study was to characterize the BrDSR (Drought Stress Resistance in B. rapa) gene and to identify the expression network of drought-inducible genes in Chinese cabbage under drought stress. Agrobacterium-mediated transformation was conducted using a B. rapa inbred line ('CT001') and the pSL100 vector containing the BrDSR full length CDS (438 bp open reading frame). Four transgenic plants were selected by PCR and the expression level of BrDSR was approximately 1.9-3.4-fold greater than that in the wild-type control under drought stress. Phenotypic characteristics showed that BrDSR over-expressing plants were resistant to drought stress and showed normal growth habit. To construct a co-expression network of drought-responsive genes, B. rapa 135K cDNA microarray data was analyzed to identify genes associated with BrDSR. BrDSR was directly linked to DARK INDUCIBLE 2 (DIN2, AT3G60140) and AUTOPHAGY 8H (ATG8H, AT3G06420) previously reported to be leaf senescence and autophagy-related genes in plants. Taken together, the results of this study indicated that BrDSR plays a significant role in enhancement of tolerance to drought conditions.

• Journal of Mechanical Science and Technology
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• v.20 no.4
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• pp.437-446
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• 2006

Laser vision inspection systems are becoming popular for automated inspection of manufactured components. The performance of such systems can be enhanced by improving accuracy of the hardware and robustness of the software used in the system. This paper presents a new approach for enhancing the capability of a laser vision system by applying hardware compensation and using efficient analysis software. A 3D geometrical model is developed to study and compensate for possible distortions in installation of gantry robot on which the vision system is mounted. Appropriate compensation is applied to the inspection data obtained from the laser vision system based on the parameters in 3D model. The present laser vision system is used for dimensional inspection of car chassis sub frame and lower arm assembly module. An algorithm based on simplex search techniques is used for analyzing the compensated inspection data. The details of 3D model, parameters used for compensation and the measurement data obtained from the system are presented in this paper. The details of search algorithm used for analyzing the measurement data and the results obtained are also presented in the paper. It is observed from the results that, by applying compensation and using appropriate algorithms for analyzing, the error in evaluation of the inspection data can be significantly minimized, thus reducing the risk of rejecting good parts.

• Microbiology and Biotechnology Letters
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• v.25 no.1
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• pp.23-29
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• 1997

The nucleotide sequence of the estI gene encoding acetylxylan esterase I of Bacillus stearothermophilus was determined and analyzed. The estI gene was found to consist of a 810 base pair open reading frame coding for a polypeptide of 270 amino acids with a deduced molecular weight of 30 kDa. This was in well agreement with the molecular weight (29 kDa) estimated by SDS-PAGE of the purified esterase. The coding sequence was preceded by a putative ribo some binding site 10 bp upsteam of the ATG codon. Further 53 bp upstream, the transcription initiation signals were identified. The putative $_{-}$10 sequence (TCCAAT) and $_{-}$35 seqence (TTGAAT) corresponded closely to the respective consensus sequences for the Bacillus subtiis major RNA polymerase. The G+C content of the coding region of the estI was 51% whereas that of the third position of codone was 60.2%. The N-terminal amino acid sequence of the EstI deduced from the nucleotide sequence perfectly matched the corresponding region of the purified esterase described previously. Comparison with the amino acid sequence of other esterases and lipases reported so far allowed us to identify a sequence, GLSMG at positions 123 to 127 of the EstI which was reported to be the highly conserved active site sequence for those enzymes. The nucleotide sequence of the estI revealed 55.7% homology to that of the xylC coding for the acetylxylan esterase of Caldocellum saccharolyticum.

• Journal of Microbiology and Biotechnology
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• v.5 no.3
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• pp.117-124
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• 1995

A gene (xynA) encoding the endo-xylanase (E.C.3.2.1.8) from Bacillus stearothermophilus was cloned in E. coli, and its complete nucleotide sequence was determined. The xynA gene consists of a 636 base pairs open reading frame coding for a protein of 212 amino acids with a deduced molecular weight of 23, 283 Da. A putative signal sequence of 27 amino acid residues shows the features comparable with the Bacillus signal sequences; namely, the signal contains a positively charged region close to the N-terminus followed by a long hydrophobic string. The coding sequence is preceded by a possible ribosome binding site with a free energy value of -16.6 kcal/mol and the transcription initiation signals are located further upstream. The translation termination codon (TAA) at the 3 end of the coding sequence is followed by two palindrome sequences, one of which is thought to act as a terminator. The xynA gene has a high GC content, especially in the wobble position of codons (64%). Comparison of the primary protein sequence with those of other xylanases shows a high homology to the xylanases belonging to family G.

• The Journal of Internal Korean Medicine
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• v.30 no.3
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• pp.465-480
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• 2009

Objective : Lonicerae Flos has detoxifying properties and been used as antipyretic, antibacterial and antitumor. Fermentation of herbal medicine is known to increase the absorption, enhance effectiveness, decrease herbal toxicity and reduce side-effects. This study was performed to measure the effects of fermented Lonicerae Flos on influenza A/WSN (H1N1) virus replication. Material and Methods : Lonicerae Flos was fermented by Lactobacillus casei PM1. Fermented Lonicerae Flos was treated for 12 hours to MDCK (Mardin Darby canine kidney) cells, then cell-virulence was observed by MTT assay for 12 hours, 24 hours, and 36 hours after treatment. Following cases were conducted for 0, 10, 100, and $1000{\mu}g/ml$ concentrations of fermented Lonicerae Flos under the same time-frame; the fermented Lonicerae Flos was treated to MDCK cells before and after contamination by A-type influenza virus. The fermented Lonicerae Flos and the virus were mixed directly. The influence was observed by MTT assay and plaque assay. Results : These findings suggest that the fermented Lonicerae Flos inhibited the virulence of influenza A virus in MDCK cells and suppressed the plaque forming colonies induced by influenza A virus. Furthermore, pretreatment with fermented Lonicerae Flos was more effective than post-treatment. The titer of influenza virus was reduced for all before and after influenza A virus inoculation.

• Structural Engineering and Mechanics
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• v.59 no.6
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• pp.1121-1137
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• 2016

A large number of structure in the world were build with poor seismic details, with or without any lateral load resisting system like concentrically braced frames and steel plate shear walls. These structures can reveal deteriorating hysteretic behaviors with stiffness and strength degradation. Therefore, seismic retrofitting of such structures for drift control has vital importance. In this study a retrofit methodology has been developed, which involves diagonal bracing of steel frames with different cable arrangements. In the experimental and numerical program 5 different lateral load resisting system were tested and results compared with each other. The results indicated that multi-cable arrangements suggested in this study showed stable ductile behavior without any sudden decrease in strength. Due to the usage of more than one diagonal cable, fracture of any cable did not significantly affect the overall strength and deformation capacity of the system. In cable braced systems damages concentrated in the boundary zones of the cables and beams. That is why boundary zone must have enough stiffness and strength to resist tension field action of cables.

• Structural Engineering and Mechanics
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• v.17 no.3_4
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• pp.539-556
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• 2004

In this paper, the design, fabrication and characterization of a piezoelectric friction damper are presented. It was sized with the proposed practical procedure to minimize the story drift and floor acceleration of an existing 1/4-scale, three-story frame structure under both near-fault and far-field earthquakes. The design operation friction force in kip was numerically determined to range from 2.2 to 3.3 times the value of the peak ground acceleration in g (gravitational acceleration). Experimental results indicated that the load-displacement loop of the damper is nearly rectangular in shape and independent of the excitation frequency. The coefficient of friction of the damper is approximately 0.85 when the clamping force on the damper is above 400 lbs. It was found that the friction force variation of the damper generated by piezoelectric actuators with 1000 Volts is approximately 90% of the expected value. The properties of the damper are insensitive to its ambient temperature and remain almost the same after being tested for more than 12,000 cycles.