• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.44 no.1
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• pp.1-9
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• 2008

This study aims to reduce the bycatch of young fishes and other species in the octopus(Octopus vulagris, Octopus dolfleini) trap fishery on the East Sea, Korea. We carried out field experiments and tank experiments to verify the fishing efficiency and bycatch with the different 8 types of trap. 4 of them had mesh size 20, 35, 55 respectively and 75mm, 2 of them with escape ring of diameter 30mm and 50mm and 2 of them with escape device. The gap was 20 and 40mm respectively. The mesh sizes of the traps with ring and frame were 20mm. The traps with mesh size 20 and 35mm were not reasonable because it could fish under 300g octopus as bycatch which is not permitted by the Korean fisheries regulations. The catch number of octopus over 300g by traps with escape ring of diameter 30mm was 53. The catch of trap with mesh size 20mm was 54. The catch of trap with mesh size 35mm was 53. There is no significant difference among them. The catch number of octopus over 500g that is fishermen's favorite one was 46 in the trap with escap ring. The diameter of was 30 mm and the catch of trap with mesh size 20mm was more 3 catch and the catch of trap with mesh size 35mm had 6 more catch. Fishermen wanted to catch over 500g octopus because the octopuses had commercial value. The traps with escape ring can be replaceable with he traps with mesh size 20 or 35mm because the trap is suitable to conserve the octopus resources and keep the fishermen's fishing substantiality.

• Horticultural Science & Technology
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• v.16 no.1
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• pp.15-17
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• 1998

In order to provide cool night to strawberry plant with cool air and water, an abandoned cool mine was utilized. It's effects on flower induction and fruit yield in 4 different cultivars were examined. After 70 days of transplanting, flowering frequency was below 65% and 100% in control and treatment of night cooling, respectively and regardless of cultivars. Number of flower buds and flower clusters were higher in treated plants compared with the control. Average time until flowering was much less in treated plants. In terms of yield weight and total yield, 'Suhong' was found to be the best cultivar, averaging 24.2g fruit. Cooling contributed to the 6-7 times of increase in total yield within the frame of harvesting times. The fruit were harvested at 80-97 days after transplanting.

• Journal of Microbiology and Biotechnology
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• v.26 no.10
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• pp.1800-1807
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• 2016

To understand how human cytomegalovirus (HCMV) might change and evolve after reactivation, it is very important to understand how the nucleotide sequence of cultured HCMV changes after in vitro passaging in cell culture, and how these changes affect the genome of HCMV and the consequent variation in amino acid sequence. Strain JHC of HCMV was propagated in vitro for more than 40 passages and its biological and genetic changes were monitored. For each passage, real-time PCR was performed in order to determine the genome copy number, and a plaque assay was employed to get virus infection titers. The infectious virus titers gradually increased with passaging in cell culture, whereas the number of virus genome copies remained relatively unchanged. A linear correlation was observed between the passage number and the log₁₀ infectious virus titer per virus genome copy number. To understand the genetic basis underlying the increase in HCMV infectivity with increasing passage, the whole-genome DNA sequence of the high-passage strain was determined and compared with the genome sequence of the low-passage strain. Out of 100 mutations found in the high-passage strain, only two were located in an open reading frame. A G-T substitution in the RL13 gene resulted in a nonsense mutation and caused an early stop. A G-A substitution in the UL122 gene generated an S-F nonsynonymous mutation. The mutations in the RL13 and UL122 genes might be related to the increase in virus infectivity, although the role of the mutations found in noncoding regions could not be excluded.

• Journal of Biosystems Engineering
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• v.43 no.2
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• pp.103-109
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• 2018

Purpose: This study has been conducted to develop a potato yield monitoring system, consisting of a segmentation algorithm to detect potatoes scattered on a soil surface and a counting system to count the number of potatoes and convert the data from two-dimensional images to masses. Methods: First, a segmentation algorithm was developed using top-hat filtering and processing a series of images, and its performance was evaluated in a stationary condition. Second, a counting system was developed to count the number of potatoes in a moving condition and calculate the mass of each using a mass estimation equation, where the volume of a potato was obtained from its two-dimensional image, and the potato density and a correction factor were obtained experimentally. Experiments were conducted to segment potatoes on a soil surface for different potato sizes. The counting system was tested 10 times for 20 randomly selected potatoes in a simulated field condition. Furthermore, the estimated total mass of the potatoes was compared with their actual mass. Results: For a $640{\times}480$ image size, it took 0.04 s for the segmentation algorithm to process one frame. The root mean squared deviation (RMSD) and average percentage error for the measured mass of potatoes using this counting system were 12.65 g and 7.13%, respectively, when the camera was stationary. The system performance while moving was the best in L1 (0.313 m/s), where the RMSD and percentage error were 6.92 g and 7.79%, respectively. For 20 newly prepared potatoes and 10 replication measurements, the counting system exhibited a percentage error in the mass estimation ranging from 10.17-13.24%. Conclusions: At a travel speed of 0.313 m/s, the average percentage error and standard deviation of the mass measurement using the counting system were 12.03% and 1.04%, respectively.

• Fisheries and Aquatic Sciences
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• v.14 no.1
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• pp.43-54
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• 2011

Gene structure of copper/zinc-superoxide dismutase (Cu/Zn-SOD; sod1) was characterized in Hemibarbus mylodon (Teleostei; Cypriniformes), an endangered freshwater fish species in Korean peninsula. Full-length cDNA of H. mylodon SOD1 consisted of a 796-bp open reading frame sequence encoding 154 amino acids, and the deduced polypeptide sequence shared high sequence homology with other orthologs, particularly with regard to metal-coordinating ligands. Genomic structure of the H. mylodon sod1 gene (hmsod1; 1,911 bp from the ATG start codon to the stop codon) was typical quinquepartite (i.e., five exons interrupted by four introns); the lengths of the exons were similar among species belonging to various taxonomic positions. The molecular phylogeny inferred from sod1 genes in the teleost lineage was in accordance with the conventional taxonomic assumptions. 5'-flanking upstream region of hmsod1, obtained using the genome walking method, contained typical TATA and CAAT boxes. It also showed various transcription factor binding motifs that may be potentially involved in stress/immune response (e.g., sites for activating proteins or nuclear factor kappa B) or metabolism of xenobiotic compounds (e.g., xenobiotic response element; XRE). The hmsod1 transcripts were ubiquitously detected among tissues, with the liver and spleen showing the highest and lowest expression, respectively. An experimental challenge with Edwardsiella tarda revealed significant upregulation of the hmsod1 in kidney (4.3-fold) and spleen (3.1-fold), based on a real-time RT-PCR assay. Information on the molecular characteristics of this key antioxidant enzyme gene could be a useful basis for a biomarker-based assay to understand cellular stresses in this endangered fish species.

• Journal of Microbiology and Biotechnology
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• v.17 no.7
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• pp.1106-1112
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• 2007

This report provides the complete nucleotide sequences of the full-length cDNA encoding squalene synthase (SQS) and its genomic DNA sequence from a triterpene-producing fungus, Ganoderma lucidum. The cDNA of the squalene synthase (SQS) (GenBank Accession Number: DQ494674) was found to contain an open reading frame (ORF) of 1,404 bp encoding a 468-amino-acid polypeptide, whereas the SQS genomic DNA sequence (GenBank Accession Number: DQ494675) consisted of 1,984 bp and contained four exons and three introns. Only one gene copy was present in the G. lucidum genome. The deduced amino acid sequence of Ganoderma lucidum squalene synthase (GI-SQS) exhibited a high homology with other fungal squalene synthase genes and contained six conserved domains. A phylogenetic analysis revealed that G. lucidum SQS belonged to the fungi SQS group, and was more closely related to the SQS of U. maydis than to those of other fungi. A gene expression analysis showed that the expression level was relatively low in mycelia incubated for 12 days, increased after 14 to 20 days of incubation, and reached a relatively high level in the mushroom primordia. Functional complementation of GI-SQS in a SQS-deficient strain of Saccharomyces cerevisiae confirmed that the cloned cDNA encoded a squalene synthase.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.12 no.1
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• pp.178-203
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• 2018

In this paper, authors have been evaluated the Frame Error Rate (FER) performance of IEEE 802.11 a/g/p standard 5 GHz frequency band WLAN over Rayleigh and Rician distributed fading channels in presence of Additive White Gaussian Noise (AWGN). Orthogonal Frequency Division Multiplexing (OFDM) based transceiver is implemented by using real-time signal processing frameworks (IEEE 802.11 Blocks) in GNU Radio Companion (GRC) and Ettus USRP N200 is used to process the symbol over the wireless radio channel. The FER is calculated for each sub-carrier conventional modulation schemes used by OFDM such as BPSK, QPSK, 16, 64-QAM with different punctuated coding rates. More precise SNR is computed by modifying the SNR calculation process of YANS and NIST error rate model to estimate more accurate FER. Here, real-time signal constellations, OFDM signal spectrums etc. are also observed to find the effect of multipath propagation of signals through flat and frequency selective fading channels. To reduce the error rate due to the multipath fading effect and Doppler shifting, channel estimation (CE) and equalization techniques such as Least Square (LS) and training based adaptive Least Mean Square (LMS) algorithm are applied in the receiver. The simulation work is practically verified at GRC by turning into a pair of Software Define Radio (SDR) as a simultaneous transceiver.

• Journal of Ocean Engineering and Technology
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• v.29 no.4
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• pp.322-330
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• 2015

As a new technical approach, a hydraulic and magnetic clamp device was developed to realize a magnetic clamp crane system by simultaneously actuating eight individual hydraulic cylinders. In this approach, an Sr-type of ferritic permanent magnet (SrO· 6Fe₂O₃), rather than the previous electromagnet, was utilized for the purpose of lifting and transporting the large curved steel plates used for manufacturing ships. This study had the goal of developing and manufacturing a hydraulic, magnetic clamp prototype composed of three main parts, including the base frame, cylinder joint, and magnet joint, in order to safely transport curved steel plates. Furthermore, this research included a performance evaluation of the manufactured prototype and acquired the purposed quantity value in the performance test. The most significant item, the magnetic adhesive force (G), was evaluated in a performance test, which utilized a ferritic permanent magnet (Sr type) with 3700~4000 G of residual induction (Br) and 2640/2770 Oe of coercive force (Hc). In particular, relevant items such as the hoist tension (kN), transportation time (s), and applied load (Kgf) on the hydraulic cylinders were also evaluated in order to determine the optimum values.

• BMB Reports
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• v.42 no.11
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• pp.731-736
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• 2009

The generation of functional recombinant antibodies from hybridomas is necessary for antibody engineering. However, this is not easily accomplished due to high levels of aberrant heavy and light chain mRNAs, which require a highly selective technology that has proven complicated and difficult to operate. Herein, we attempt to use an alkaline phosphate (AP)-fused form of single-chain variable fragment (scFv) for the simple identification of a hybridoma-derived, functional recombinant antibody. As a representative example, we cloned the scFv gene from a hybridoma-producing mouse IgG against branched-chain keto acid dehydrogenase complex-E2 (BCKD-E2) into an expression vector containing an in-frame phoA gene. Functional recombinant antibodies were easily identified by conventional enzyme-linked immunosorbent assay (ELISA) by employing scFv-AP fusion protein, which also readily serves as a valuable immuno-detective reagent.

• BMB Reports
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• v.29 no.2
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• pp.156-162
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• 1996

Transcription of hepatitis B viral pregenomic promoter is known to be regulated mainly by the combined interaction of enhancers I, II and the intervening regulatory sequences between the two enhancers. A positive regulatory element was identified by serial deletion and measuring the linked chloramphenicol acetyltransferase (CAT) activities, which overlapped with the 5' region of the X open reading frame. When the positive regulatory element was inserted upstream of the SV40 early promoter, it elevated SV40 promoter activity in HepG2 cells. Two cellular proteins of 110 (p110) and 33 (p33) kDa interacted with the positive element and both of them were present in the nucleus, but p110 also existed in the cytoplasm in phosphorylated form. Dephosphorylation of p110 by acid phosphatase enhanced the DNA-binding activity of p110. The p33 could bind to single-strand DNA specifically as well as to double-strand DNA.