• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 1999년도 임시총회 및 추계 학술발표회
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• pp.38.1-38
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• 1999

• 한국수의병리학회:학술대회논문집
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• 한국수의병리학회 2004년도 추계 학술대회 초록집
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• pp.45-45
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• 2004

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2000년도 추계 학술발표회
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• pp.21.1-21
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• 2000

• 한국수의병리학회:학술대회논문집
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• 한국수의병리학회 2005년도 Proceedings of The 2nd Asian Society of Veterinary Pathology Symposium(Vol.2) and 2005 Annual Meeting of The Korean Society of Veterinary Pathology(Vol.9)
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• pp.70-71
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• 2005

• 대한수의학회지
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• 제50권3호
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• pp.221-229
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• 2010

Encephalomyocarditis virus (EMCV) belongs to the genus Cardiovirus within the family Picornaviridae. EMCV has been recognized either as a cause of mortality in young pigs, due to acute myocarditis, or of reproductive failure in sows. An EMCV K3 strain was isolated from the heart and brain in a mummified and aborted swine fetus in 1989. For the molecular characterization of the poly(C)-tract of EMCV Korean isolates, K3 strain, viral RNA was extracted and digested with RNase T1, and analyzed the length of the poly(C)-tract by polyacrylamide gel electrophoresis. The poly(C) regions also were amplified by RT-PCR and sequenced. The present study shows that K3 strain of EMCV had a short polymorphic poly(C) tracts (5 to 30 C's) with sequences consisting of $C_9$, $C_{10}$, $C_{13}$, $C_{14}$, $C_{16}$, $C_{20}$, $CUC_{11}$, $C_8UCUC_3UC_{10}$, $C_9UCUC_3UC_{10}$, $C_{10}UCUC_3UC_{10}$, etc. These polymorphism of poly(C)-tracts of EMCV K3 strain implies the historical information of in vivo and/or in vitro passage.

• The Plant Pathology Journal
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• 제30권4호
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• pp.440-444
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• 2014

Iris yellow spot virus (IYSV) is a plant pathogenic virus which has been reported to continuously occur in onion bulbs, allium field crops, seed crops, lisianthus, and irises. In South Korea, IYSV is a "controlled" virus that has not been reported, and inspection is performed when crops of the genus Iris are imported into South Korea. In this study, reverse-transcription polymerase chain reaction (RT-PCR) and nested PCR inspection methods, which can detect IYSV, from imported crops of the genus Iris at quarantine sites, were developed. In addition, a modified positive plasmid, which can be used as a positive control during inspection, was developed. This modified plasmid can facilitate a more accurate inspection by enabling the examination of a laboratory contamination in an inspection system. The inspection methods that were developed in this study are expected to contribute, through the prompt and accurate inspection of IYSV at quarantine sites to the plant quarantine in South Korea.

• 한국수의병리학회지
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• 제3권1호
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• pp.61-64
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• 1999

A dead marten(Martes melampus) showing cough, ataxia and convulsion of hind limb followed by seizures, was submitted for diagnosis to the Pathology Division of the National Veterinary Research and Quarantine Service. In the gross lesions, lung was congested and consolidated and meningeal blood vessels were mildly congested. Histopathologic findings were diffuse interstitial pneumonia and nonsuppurative meningoencephalitis with malacia of cerebral and cerebellar white matter. Eosinophilic inclusion bodies were observed in neurons and astrocytes and oligodendroglial cells of brain and transitional epithelium of kidney. Using FA test and PCR method, specific antigens of canine distemper virus were demonstrated in the brain.

• Mycobiology
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• 제40권3호
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• pp.205-207
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• 2012

A fungus detected from the importing seeds of Papaver rhoeas under plant quarantine inspection in Korea was identified as Brachycladium penicillatum Corda. It differed in morphological characteristics from a similar species, B. papaveris, which was known to form no macroconidiophores and no microsclerotia. Since the first interception in 2006, this fungus has frequently been found from importing seeds of Papaver spp. It was detected from 31 out of 282 seed consignments imported from 2006 to 2011. To prevent its introduction to Korea, the seed consignments infested by B. penicillatum were destroyed or reshipped.

• 대한수의학회지
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• 제55권2호
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• pp.105-110
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• 2015

A real-time PCR assay using hybridization probe (HybProbe) has been developed to detect Brucella (B.) melitensis strains. The primer and HybProbe sets were designed based on the gap gene of chromosome I with a specific single nucleotide polymorphism of B. melitensis. Specificity of the assay was confirmed by comparison to reference Brucella species and other related strains. In the melting curve analysis, B. melitensis generated a peak at $67^{\circ}C$ unlike those for other Brucella species observed at $61^{\circ}C$. Sensitivity of the assay for B. melitensis ranged from 20 ng to 200 fg of genomic DNA. The ability to identify 94 Mongolian B. melitensis isolates using the real-time PCR assay was identical to that of classical biotyping methods and differential multiplex PCR. These data showed that this new molecular technique is a simple and quick method for detecting B. melitensis, which will be important for the control and prevention of brucellosis.

• 한국동물위생학회지
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• 제39권1호
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• pp.65-68
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• 2016

Multiple enlarged submandibular nodules were discovered during a routine antimortem examination at slaughter in 3 dairy cows of 20-month-old. Grossly, submandibular nodules were firm, reddish, and oval in shape. On cut surface, multiple yellowish abscesses in the parenchyma were observed. Histopathologically, the nodules were characterized by severe diffuse pyogranulomatous inflammation and chronic fibrosis with multiple eosinophilic clubs of Splendore-Hoeppli material containing central bacterial colonies. Trueperella pyogenes was isolated from the lesions. This case was diagnosed as submandibular pyogranuloma caused by Trueperella pyogenes in slaughtered dairy cows in Korea.