• Proceedings of the Korean Society of Broadcast Engineers Conference
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• 2011.11a
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• pp.34-35
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• 2011

우면산 산사태로 인한 EBS 방송중단으로 방송통신재난관리의 필요성에 대한 관심이 높아지고 있다. 통신재난관리는 전기통신기본법에 의하여 규정되어 지금까지 잘 수행되어 왔으나 방송재난관리는 명확한 법 규정 없이 각 방송사 내부적으로 수행하여 왔다. 그러나 방송통신융합에 따른 방송통신발전 기본법 시행으로 방송통신재난관리 규정이 신설되어 방송사들의 재난관리 업무가 법적으로 명확히 규정되었다. 본 논문에서는 방송통신발전 기본법 시행에 따라 방송재난이라는 개념을 정의하고, 주요방송사업자(KBS, MBC, SBS, YTN, MBN)의 방송통신재난관리 기본계획, 방송재난 대비, 방송재난 보고, 방송통신재난대책본부와 관련된 법 조항을 분석하였다. 통신사업자들의 경우는 재난방송 의무는 없으나 재난방송협의회 참가를 통하여 CBS 등과 같은 재난방송에 도움을 줄 수 있도록 하는 것이 필요하다.

• Journal of Microbiology and Biotechnology
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• v.23 no.7
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• pp.984-992
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• 2013

The entire nucleotide sequence of the xylose reductase (XR) gene in Candida milleri CBS8195 sourdough yeast was determined by degenerate polymerase chain reaction (PCR) and genome walking. The sequence analysis revealed an open-reading frame of 981 bp that encoded 326 amino acids with a predicted molecular mass of 36.7 kDa. The deduced amino acid sequence of XR of C. milleri was 64.7% homologous to that of Kluyveromyces lactis. The cloned XR gene was expressed in Saccharomyces cerevisiae, and the resulting recombinant S. cerevisiae strain produced xylitol from xylose, indicating that the C. milleri XR introduced into S. cerevisiae is functional. An enzymatic activity assay and semiquantitative reverse transcription-PCR revealed that the expression of CmXR was induced by xylose. The GenBank Accession No. for CmXR is KC599203.

• Proceedings of the KIEE Conference
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• 2003.07b
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• pp.828-830
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• 2003

AVR은 동기발전기의 출력전압을 부하변동에 관계없이 일정하게 제어하며 발전기 및 시스템 보호를 위한 각 종 제어 알고리즘을 구비하고 있다. 종래의 아나로그타입 AVR은 제어설정이 불편하고 제어신뢰성이 떨어지기 때문에 점차 디지털 타입으로 교체되고 있다. 본 연구는 선박발전기에 적용하기 위한 디지털 AVR 개발에 관한 것이다. TI사의 TMS320LF2407 CPU를 사용하여 제어 H/W를 개발했으며, 과여자/저여자제어, Droop제어, 무부하/역률제어 등 다양한 제어기능을 S/W로 구비하여 부하에 따른 적용의 유연성을 높였다. 그리고 전력회로는 IGBT를 사용하여 고속 스위칭 제어함으로써 SCR 방식에 비해 제어 속응성을 개선하였다. 개발 된 제품은 AVR, 직류전원공급장치 및 부하나 계통의 사고시 보호를 위한 전류부스트(CBS)기능을 포함하고 있다.

• Journal of Microbiology and Biotechnology
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• v.11 no.4
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• pp.564-569
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• 2001

This study was carried out in order to investigate the characteristics of xylitol fermentation by a xylitol dehydrogenase defective mutant PXM-4 of P stipitis CBS 5776 and to determime optimum conditions for the high yield ofxylitol production from xylose. Gluconic acid was selected as a co substrate for the xylitol fermentation, since gluconic acid neither blocked xylose transport nor repressed xylose reductase expression. An increase of gluconic acid concentration reduced the rates of xylitol production and cell growth by decreasing medium pH, and the optimal concentration of gluconic acid was determined to be 20 gll with approximately 100% xylitol conversion yield. A fed-batch cell culture resulted in a 44.8 g/l xylitol concentration with 100% yield, based on the amount of xylose consumed.

• Journal of Microbiology and Biotechnology
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• v.1 no.3
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• pp.151-156
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• 1991

In order to construct a yeast strain having high ethanol tolerance together with good lactose fermentation ability, the protoplast fusion using Saccharomyces cerevisiae STV 89 and Kluyveromyces fragilis CBS 397 was carried out. Auxotrophic mutants of K. fragilis were obtained as a selection marker by treatment of ethylmethane sulfonate. The best mutant for protoplast fusion was selected based on the capabilities of ${\beta}-galactosidase$ production and lactose fermentation. The protoplast fusion using polyethylene glycol and calcium chloride solution led to the fusion frequence of $3{\times}10^{-6}$ and a number of fusants were obtained. Among these fusants, a fusant F-3-19 showed the best results in terms of ethanol tolerance, ${\beta}-galactosidase$ activity and lactose fermentation. The performance of lactose fermentation and ethanol tolerance by this fusant were better than those of K. fragilis. Study on the ethanol tolerance having relation to fatty acid composition and intracellular ethanol concentration revealed that the fusant F-3-19 had a higher unsaturated fatty acids content and accumulated less amount of intracellular ethanol compared with a parent of K. fragilis.

• Journal of Microbiology and Biotechnology
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• v.28 no.11
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• pp.1937-1945
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• 2018

Malassezia pachydermatis is a commensal yeast found on the skin of dogs. However, M. pachydermatis is also considered an opportunistic pathogen and is associated with various canine skin diseases including otitis externa and atopic dermatitis, which usually require treatment using an azole antifungal drug, such as ketoconazole. In this study, we isolated a ketoconazole-resistant strain of M. pachydermatis, designated "KCTC 27587," from the external ear canal of a dog with otitis externa and analyzed its resistance mechanism. To understand the mechanism underlying ketoconazole resistance of the clinical isolate M. pachydermatis KCTC 27587, the whole genome of the yeast was sequenced using the PacBio platform and was compared with M. pachydermatis type strain CBS 1879. We found that a ~84-kb region in chromosome 4 of M. pachydermatis KCTC 27587 was tandemly quadruplicated. The quadruplicated region contains 52 protein coding genes, including the homologs of ERG4 and ERG11, whose overexpression is known to be associated with azole resistance. Our data suggest that the quadruplication of the ~84-kb region may be the cause of the ketoconazole resistance in M. pachydermatis KCTC 27587.

• Journal of Digital Convergence
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• v.15 no.12
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• pp.461-468
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• 2017

'Beauty and the Beast' was generated as a novel in 1740 and descended to date with the musical movie in 2017. This study is to understand what contents has been derived and expanded from one story for 277 years upon analyzing 'Beauty and the Beast' with the background of the times. Due to the limitation to include all the folktales and stories, the subjects of this study are limited with the contents that had been known and recorded as 'Beauty and the Beast.' 'Beauty and the Beast' has been expanded to 7 types of contents including novel, fairytale, movie, drama, animation, musical and film opera. It was created as a novel in the eighteenth century, made as a fairytale, and developed as a movie by Jean Cocteau, a movie director. With Industrial Revolution and the development of science, 'Beauty and the Beast' which derived into animation, drama, and musical contents, has been developed as the film opera thanks to the new trial of Philip Glass and the development of music industry. 'Beauty and the Beast' is still regenerating with a variety of contents continuously upon the development of computer technology and the fad of global remake. With anticipation of further analytical studies on 'Beauty and the Beast,' it is expected that the data of this study can be contributed as a reference in the other studies.

• KSBB Journal
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• v.25 no.4
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• pp.401-407
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• 2010

We established a strategy for bioethanol production using the hydrolysate of rape stem, in which the inhibitor cocktail was added intentionally. The final goal of this study was to circumvent the detoxification process when the hydrolysate of lignocelluloisic biomass contained the toxic substances in high concentration. When six yeast strains were examined, Sacchromyces cerevisiae ATCC 96581 and Pichia stipitis CBS 7126 were relatively resistant to inhibitor cocktail. Then, using strains 96581 and 7126, we designed a process strategy for bioethanol production, assuming that the concentration of toxic substance in the hydrolysate of rape stem was remarkably high. When strains 96581 and 7126 were inoculated simultaneously, it was observed that strain 7126 produced bioethanol as well as strain 96581, although the concentration of inhibitor cocktail was 18.2% (v/v). Finally, throughout this co-cultivation of strains 96581 and 7126, bioethanol was produced about 6.0 (g/L), and bioethanol yield reached at 0.4 (g-bioethanol/g-reducing sugar) (78.4% of theoretical value).

• Journal of Microbiology and Biotechnology
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• v.24 no.12
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• pp.1606-1608
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• 2014

During an investigation of the fungal diversity of Korean soils, four Penicillium strains could not be assigned to any described species. The strains formed monoverticillate conidiophores with occasionally a divaricate branch. The conidia were smooth or finely rough-walled, globose to broadly ellipsoidal and $2.5-3.5{\times}2.0-3.0{\mu}m$ size. Their taxonomic novelty was determined using partial ${\beta}$-tubulin gene sequences and the ribosomal internal transcribed spacer region. The phylogenetic analysis showed that the isolates belonged to section Lanata-Divaricata and were most closely related to Penicillium raperi. Phenotypically, the strains differed from P. raperi in having longer and thicker stipes and thicker phialides. Strain KACC $47721^T$ from bamboo field soil was designated as the type strain of the new species, and the species was named Penicillium koreense sp. nov., as it was isolated from various regions in Korea.

• Proceedings of the KIPE Conference
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• 2008.06a
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• pp.136-138
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• 2008

The output voltage of synchronous generator is regulated constantly by field current control in excitation system. Most of ship generator exciter system uses the thyristor phase controlled rectifier. However this rectifier is difficult to realize that the speed control system because its control period is slower than MOSFET and IGBT type converter. Therefore, this paper deals with PMG(Permanent Magnet Generator) type digital excitation system using MOSFET for ship synchronous generator. The organization of this excitation system is very simple. When the generator is under the short circuit accident, the output voltage becomes zero state and AVR can not operate. Thus generator requires the function for flowing output current through CBS. The performance of the proposed system is evaluated on a 10kVA experimental prototype circuit in place of real ship generator.