• Title/Summary/Keyword: K-CBS

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Nucleotide Sequence of $\alpha$-Amylase Gene in the Yeasr Schwanniomyces accidentalis var. persoonii CBS 2169 (Schwanniomyces occidentalis var. persoonii CBS 2169 $\alpha$-Amylase 유전자의 Nucleotide Sequence)

  • Park, Jong-Chun;Bai, Suk;Oh, Sang-Jin;Lee, Jin-Jong;Chun, Soon-Bai
    • Microbiology and Biotechnology Letters
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    • v.21 no.6
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    • pp.534-541
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    • 1993
  • The relationship between Schwanniomyces occidentalis CBS 2863 (formerly castellii) and CBS 1153 (formerly alluvius), and their variety persoonii was examined at alpha-amylase gene level. Using Sch. occidentalis alpha-amylase gene as probe, Sch. occidentalis alpha-amylase gene homologues were obtained from Sch. occidentalis CBS 1153 and Sch. occidentalis var. persoonii. The restriction analysis of these homologues showed that the restriction enzyme sites between Sch. occidentalis CBS 2863 and CBS 1153 was identical but different between these strains and Sch. occidentalis var. persoonii.

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Engineering Characteristics of Blast Furnace Slag Cement Mortar Using Chlorine Bypass System-Dust as Alkali Activator (Chlorine Bypass System-Dust를 알칼리 자극제로 사용한 고로슬래그 시멘트 모르타르의 공학적 특성)

  • Han, Min-Cheol;Lee, Dong-Joo
    • Journal of the Korean Recycled Construction Resources Institute
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    • v.8 no.2
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    • pp.235-244
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    • 2020
  • This study conducted a series of studies to offer a novel method of using CBS-dust that produced as by-product in the manufacture of cement. Four different contents of BS and CBS-dust were adopted for test parameters of this study. Mortar with 50% of W/B was fabricated. First, in the case of the fresh mortar, the flow decreased as the CBS-dust replacement rate increased, but the binder composition ratio BS 45% and 65% showed higher fl ow than Pl ain when repl acing CBS-dust 5%. In the case of air content, overall, the tendency was proportional to the CBS-dust replacement rate, and chloride tended to exceed the reference value at all replacement rates except for the CBS-dust 0% replacement. The compressive strength of the hardened mortar shows the resul t that the strength is improved when the CBS-dust is repl aced by 5% to 10%, and the CSH gel and structure formation is confirmed by microstructure analysis through the hydration reaction when the CBS-dust is replaced. Therefore, for a given condition CBS-dust is used as a early-strength admixture in a concrete secondary product that uses a large amount of admixture without reinforcing bars it can be an effective method for enhancing the strength of concrete as an alkali activator.

Electrochemical Performance of Activated Carbons/Mn3O4-Carbon Blacks for Supercapacitor Electrodes

  • Kim, Ki-Seok;Park, Soo-Jin
    • Bulletin of the Korean Chemical Society
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    • v.34 no.8
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    • pp.2343-2347
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    • 2013
  • In this work, manganese dioxide ($Mn_3O_4$)/carbon black (CB) composites (Mn-CBs) were prepared by an in situ coating method as electrical fillers and the effect of the Mn-CBs on the electrical performance of activated carbon (AC)-based electrodes was investigated. Structural features of Mn-CBs produced via in situ coating using a $KMnO_4$ solution were confirmed by XRD and TEM images. The electrical performances, including cv curves, charge-discharge behaviors, and specific capacitance of the ACs/Mn-CBs, were determined by cyclic voltammograms. It was found that the composites of $Mn_3O_4$ and CBs were successfully formed by in situ coating method. ACs/Mn-CBs showed higher electrical performance than that of AC electrodes fabricated with conventional CBs due to the pesudocapacitance reaction of manganese oxides in the aqueous electrolyte. Consequently, it is anticipated that the incorporation of $Mn_3O_4$ into CBs could facilitate the utilization of CBs as electrical filler, leading to enhanced electrochemical performance of AC electrodes for supercapacitors.

Recombinant Adeno-associated Virus-Mediated Gene Transfer in Homocystinuria Mice (호모시스틴뇨증 동물 모델의 유전자 치료)

  • Park, Eun-Sook
    • Journal of The Korean Society of Inherited Metabolic disease
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    • v.5 no.1
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    • pp.9-17
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    • 2005
  • Homocystinuria is a metabolic disorder caused by a deficiency of cystathionine ${\beta}$-synthase (CBS). Patients with homocystinuria show clinical symptoms such as mental retardation, lens dislocation, vascular disease with life-threatening thromboembolisms and skeletal deformities. Generally, the major treatments for CBS deficiency include pharmacologic doses of pyridoxine or dietary restriction of methionine. However, there is no effective treatment for this disease up till today and gene therapy can be an attractive novel approach to treatment of the disease. We investigated whether a recombinant adeno-associated virus could be used as a CBS gene transfer vector to reduce the excessive homocysteine level in the homocystinuria mouse model. Recombinant adeno-associated virus vector encoding the human CBS gene (rAAV-hCBS), driven by EF1-a promoter, was infused into CBS-deficient mice ($CBS^{-/-}$) via intramuscular (IM) and intraperitoneal (IP) injection. IP injection was more efficient than IM injection for prolongation of lives and reduction of plasma homocysteine levels. After 2 weeks of gene transfer by IP injection, serum homocysteine level was significantly decreased in treated mice compared with the age-matched controls and the life span was extended about 1.5 times. Also, increased expression of CBS gene was observed by immunohistochemical staining in livers of treated $CBS^{-/-}$ mice and microvesicular lipid droplets was decreased in cytoplasm of liver. These results demonstrate the possibility and efficacy of gene therapy by AAV gene transfer in homocystinuria mice.

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Antimicrobial and Antioxidant Peptide from Bacillus Strain CBS73 Isolated from Korean Food

  • Kim, Miri;Khan, Md Maruf;Yoo, Jin Cheol
    • Journal of Integrative Natural Science
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    • v.10 no.3
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    • pp.154-161
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    • 2017
  • An antimicrobial peptides-producing Bacillus strain CBS73 was isolated from fermented food (kimchi) that produces low-molecular-weight proteins with broad-spectrum antimicrobial activity. Our goal was to explore the therapeutic potential of antimicrobial substances produced by Bacillus species. Peptide CBS73 was purified from Bacillus subtilis subsp. subtilis with identity of 99.79%. It was found to be stable at pH 4.0-10.0 and temp $20-60^{\circ}C$. A protein band around 5.2 kDa was detected in tricine-SDS-PAGE and band was confirmed by MALDI-TOF test. Peptide CBS73 showed antimicrobial activity against MDR bacteria. The minimal inhibitory concentration (MIC) of peptide CBS73 for vancomycin-resistant S. aureus (VRSA), vancomycin resistant Enterococci (VRE) and Salmonella typhimurium ranged from $10-40{\mu}g/mL$. The antioxidant activity of peptide CBS73 was measured by DPPH scavenging, reducing power activity and total phenolic content. Cell viability and NO production result showed less cytotoxic effect upto $12{\mu}g/mL$. Peptide CBS73 could be a promising antimicrobial agent for clinical application.

The Comparison Between CBS and Pencil and Paper Test in Patients With Unilateral Neglect (편측무시 환자에서 관찰을 통한 Catherine Bergego Scale과 지필 검사 간 비교)

  • Heo, Eun-Young;Kwon, Hyuk-Cheol;Hwang, Ki-Cheol
    • Therapeutic Science for Rehabilitation
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    • v.2 no.1
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    • pp.48-56
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    • 2013
  • Purpose : To identify the correlation between CBS and pencil and paper test, and to test the sensitivity of above tests about unilateral neglect. Mostly used tool of pencil and paper test is line bisection and albert test. CBS is behavioral neglect test. Methods : The subjects of study were 44 stroke patients with left hemiplegia. Data were analyzed for Pearson correlation, paired t-test and ANOVA using SPSS program. The significance level was ${\alpha}<.01$. Results : The results of the study was as follow: 1. CBS had a significant correlation of line bisection and Albert test. 2. CBS was more sensitive than the pencil and paper in unilateral neglect. 3. CBS had high correlations with ADL than the pencil and paper test. Conclusion : The result showed that CBS was more useful than a pencil and paper test to distinguish unilateral neglect and available to diagnose anosognosia. Therefore, it should be considered applying CBS to inspect and cure unilateral neglect.

An Implementation Method of Cycle Accurate Simulator for the Design of a Pipelined DSP

  • Park, Hyeong-Bae;Park, Ju-Sung;Kim, Tae-Hoon;Chi, Hua-Jun
    • JSTS:Journal of Semiconductor Technology and Science
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    • v.6 no.4
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    • pp.246-251
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    • 2006
  • In this paper, we introduce an implementation method of the CBS (Cycle Base Simulator), which describes the operation of a DSP (Digital Signal Processor) at a pipeline cycle level. The CBS is coded with C++, and is verified by comparing the results from the CBS and HDL simulation of the DSP with the various test vectors and application programs. The CBS shows the data about the internal registers, status flags, data bus, address bus, input and output pin of the DSP, and also the control signals at each pipeline cycle. The developed CBS can be used in evaluating the performance of the target DSP before the RTL(Register Transfer Level) coding as well as a reference for the RTL level design.

Validation of the Korean Version of the Continuing Bonds Scale (한국판 지속 유대 척도의 타당화)

  • Kyeyang Kim ;Jongwon Park ;Wan-Suk Gim
    • Korean Journal of Culture and Social Issue
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    • v.22 no.2
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    • pp.263-283
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    • 2016
  • The present study aimed at examining the factor structure, reliability and validity of the Korean version of the Continuing Bonds Scale (K-CBS). In study 1, exploratory factor analysis was administered to 293 bereaved adults who had experienced the death of a loved one, and it revealed a single factor structure with 10 items that explained 52.59% of the total variance. The K-CBS showed good internal consistency with Cronbach's alpha of .92. In study 2, confirmatory factor analysis in a different sample of 200 bereaved adults indicated satisfactory standardized regression weights of all items. However, one item had a squared multiple correlation less than .40, hence, this item was discarded, and 9 items remained for the final scale. The single factor model with 9 items displayed a good fit. The K-CBS had strong positive correlation with grief symptoms, and weak positive correlation with depression. After controlling for grief, however, the K-CBS was predictive of a decrease in depression. The K-CBS was positively associated with posttraumatic growth. In addition, significant differences in scores of the K-CBS were shown among groups based on the deceased's relation to the bereaved and expectedness of loss. These results suggest that the K-CBS is a reliable and valid instrument to measure continuing bonds. Finally, implications, limitations, and directions for future research were discussed.

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A Synchronous Cooperative Communication for Emergency Alert Broadcast Based on Cellular Systems (이동통신 기반의 재난경보 방송을 위한 동기식 협력통신 방식)

  • Chang, Sekchin
    • Journal of Broadcast Engineering
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    • v.19 no.2
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    • pp.184-194
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    • 2014
  • The CBS methodology has been utilized in order to make a rapid broadcast of emergency alert based on cellular systems. We present a synchronous cooperative communication method for the CBS. Especially, we suggest a synchronization scheme and a data recovery approach for high-rate cooperative communications. For the high-rate transfer of emergency alert, the cyclic prefix is added to the preamble for the synchronization. For the data recovery, the Alamouti technique is utilized on frequency domain, which is similar to SC-FDE. The simulation results confirm that our proposed scheme is very suitable for the CBS.

A Delivery Scheme of CBS Alert Message for 3G Cellular Phone in indoor Environment (실내 공간에서 3G 이동단말기를 위한 CBS 재난 정보 전송)

  • Song, Mihwa;Chang, Sekchin
    • Proceedings of the Korean Society of Broadcast Engineers Conference
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    • 2013.06a
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    • pp.204-205
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    • 2013
  • 최근 일본의 지진 쓰나미 등 전 세계적으로 많은 재난이 증가하고 있고, 국내에서는 신속한 재난 정보 전송을 위해서 CBS(Cell Broadcast Service) Alert Message를 국가 표준으로 제정하였다. CBS를 통한 전송은 지역단위 전송이 가능하므로 재난이 발생한 지역에 신속한 전송 서비스를 가능하게 해준다. 하지만 국내의 경우 CBS Alert Message가 LTE(Long Term Evolution) 단말에서만 수신이 가능하고 3G 단말에서는 수신이 불가능하다. 이에 본 논문은 실내 환경에서 gateway-AP(Access Point)를 이용하여 3G 단말이 CBS Alert Message를 수신 할 수 있는 방법을 제시하려고 한다.

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