• Microbiology and Biotechnology Letters
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• v.44 no.2
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• pp.140-144
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• 2016

The gene encoding chondroitinase ABC from a genomic library of Bacteroides stercoris HJ-15, which was isolated from human feces, was cloned. The cloned gene consisted of 3,090 bp and was predicted to encode a 1,029−amino-acid protein. The B. stercoris chondroitinase ABC gene was not homologous to other chondroitinase ABC genes; however, its amino acid sequence showed 71% homology to that of Bacteroides thetaiotaomicron. The gene was cloned in the pET-26b+ expression vector and expressed under the T7 promoter in Escherichia coli BL21(DE3). The purified recombinant chondroitinase ABC degraded chondroitin sulfates A, B, and C.

• The Plant Pathology Journal
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• v.30 no.4
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• pp.375-383
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• 2014

Fungi tolerate exposure to various abiotic stresses, including cytotoxic compounds and fungicides, via their ATP-driven efflux pumps belonging to ATP-binding cassette (ABC) transporters. To clarify the molecular basis of interaction between the fungus and various abiotic stresses including fungicides, we constructed a cDNA library from germinated conidia of Colletotrichum acutatum, a major anthracnose pathogen of pepper (Capsicum annum L.). Over 1,000 cDNA clones were sequenced, of which single clone exhibited significant nucleotide sequence homology to ABC transporter genes. We isolated three fosmid clones containing the C. acutatum ABC1 (CaABC1) gene in full-length from genomic DNA library screening. The CaABC1 gene consists of 4,059 bp transcript, predicting a 1,353-aa protein. The gene contains the typical ABC signature and Walker A and B motifs. The 5'-flanking region contains a CAAT motif, a TATA box, and a Kozak region. Phylogenetic and structural analysis suggested that the CaABC1 is a typical ABC transporter gene highly conserved in various fungal species, as well as in Chromista, Metazoans, and Viridiplantae. We also found that CaABC1 was up-regulated during conidiation and a minimal medium condition. Moreover, CaABC1 was induced in iprobenfos, kresoxim-methyl, thiophanate-methyl, and hygromycin B. These results demonstrate that CaABC1 is necessary for conidiation, abiotic stress, and various fungicide resistances. These results will provide the basis for further study on the function of ABC transporter genes in C. acutatum.

• Journal for History of Mathematics
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• v.21 no.3
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• pp.113-126
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• 2008

This work is devoted to study about the abc conjecture: how it works in the development of the proof of Fermat's last theorem and more generally in the diophantine equation theory. And it is also studied the application of the abc conjecture to the iteration of polynomials.

• Journal of Life Science
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• v.20 no.2
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• pp.176-182
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• 2010

Magnaporthe oryzae is destructive plant-pathogenic fungus and causes rice blast. The pathogen uses several mechanisms to circumvent the inhibitory actions of fungicides. ATP-binding cassette (ABC) transporters are known to provide protection against toxic compounds in the environment. PC facilitated bioinformatic analysis, particularly with respect to accessing and extracting database information and domain identification. We predicted ABC transporter genes from the M. oryzae genome sequence with computation and bioinformatics tools. A total of thirty three genes were predicted to encode ABC transporters. Three of thirty three putative genes corresponded to three known ABC transporter genes (ABC1, ABC2 and ABC3). Copy numbers of the ABC transporter genes were proven by Southern blot analysis, which revealed that twenty genes tested exist as a single copy. We amplified the DNA complementary to RNA corresponding to eleven of these by reverse transcriptase polymerase chain reaction.

• Fire Science and Engineering
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• v.32 no.3
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• pp.1-7
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• 2018

This study the effect of pyrolysis products ABC dry chemical and of monoammonium phosphate on wood surface. When the pyrolysis product was removed from the wood surface, monoammonium phosphate was removed due to the high viscosity of the transparent pyrolysis product, but the ABC dry chemical was removed in a lump form. Thermal analysis showed that the pyrolysis characteristics of each sample were similar but the weight of pyrolysis residue was 55.9% for ABC dry chemical and 25.2% for monoammonium phosphate. The additives added to the ABC dry chemical also affect the weight of the pyrolysis residue and the fire protection effect of metaphosphoric acid.

• Journal of Environmental Health Sciences
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• v.39 no.4
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• pp.369-375
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• 2013

Objectives: This study was conducted to identify factors determining the toxicity of manufactured silver nano-particles (AgNPs) on aquatic organisms. Methods: For this purpose, we prepared several AgNPs with varied characteristics, including hydrodynamic size (nano-$^{ABC}Ag^{Cit}\;vs$-sized-$^{ABC}Ag^{Cit}$), impurities ($^{ABC}Ag$ stock vs $^{ABC}Ag$), and citrate capping ($^{ABC}Ag^{Cit}$), using a commercially available manufactured AgNP ($^{ABC}Ag$ stock). Acute tests were conducted using larval zebrafish (Danio rerioI). In addition, in order to determine the ecotoxicological potentials of various capping agents, toxicity tests were conducted with microbes, waterfleas, and fish for eight different capping agents that are used for NPs. Results: The toxicity of AgNPs in terms of 96 h fish $LC_{50}$ increased in the following order: $^{ABC}Ag$ stock < $^{ABC}Ag=^{ABC}Ag^{Cit}=nano-^{ABC}Ag^{Cit}$ < ${\mu}$-sized-$^{ABC}Ag^{Cit}$ < $AgNO_3$. After removing impurities by dialysis, 96 h $LC_{50}$ value decreased significantly from $126.6{\mu}g/L$ (95% confidence intervals [CI]: 107.0-146.2) ($^{ABC}Ag$ stock) to $78.6{\mu}g/L$ (CI: 72.7-84.8) ($^{ABC}Ag$). For ${\mu}$-sized-$^{ABC}Ag^{Cit}$ (ranging between 3.9 and 40.6 nm) and $^{ABC}Ag^{Cit}$ (40.6 nm and $9.1{\mu}m$), the 96 h $LC_{50}$ of the former ($43.9{\mu}g/L$, CI: 36.0-51.7) was approximately two-fold lower than that of the latter ($87.0{\mu}g/L$, CI: 73.5-100.3). Conclusions: In this study, we found that for acute lethality, the contribution of impurities and particle size was significant, but that of citrate was negligible.

• Journal of Gifted/Talented Education
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• v.19 no.2
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• pp.241-260
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• 2009

The purpose of this study was to contrast young gifted children with ordinary young children in K-ABC profile. The subject were 51 young gifted children and 51 ordinary young children, 2 to 4 years of age. Data of children's K-ABC profile were analyzed by Correlation and Crosstabs. The main results of this study were as follows: First, in the case of ordinary young children, there were significant positive correlation among 'Mental Processing Composite' and all sub-tests of mental processing composite except 'face memory' test, 'Achievement Scale'. In young gifted children, there were significant positive correlation among 'Mental Processing Composite' and just four sub-tests of mental processing composite, and there were no significant correlation between 'Mental Processing Composite' and 'Achievement Scale'. Second, there were no significant differences among all sub-tests' strength and weakness in young gifted children and ordinary young children. Third, young gifted children got higher score in 'Sequential Processing Scale' and 'Mental Processing Composite' than 'Achievement Scale'. But in ordinary young children, there were no significant differences among all K-ABC' sub-scales.

• Korea Journal of Hospital Management
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• v.10 no.1
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• pp.25-47
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• 2005

This study was purposed to find out the difference of the accounting of practical cost between the ABC system and the traditional costing system applied in a hospital, to verified general effect of ABC. Methods: This case study deals with the method of calculation, the cost information that is produced at K hospital in Busan. To examine ABC system and traditional costing system, applying them to the clinical pathology, radiology, physics in K hospital. Results: As a result of costing analysis, it is showed maximum difference of 50% between ABC and traditional cost. compared in revenue center, it occurs the difference of 15% of them. considering the result, it is confirmed that ABC could be used as a means to offer more precise information. therefore, ABC makes possible to produce precise costing information and grasp the driver of cost, and it is possible to reduce cost effectively. Conclusion: ABC provide six benefits: (1) more accurate of service delivered (2) inproved pricing and contracting strategies (3) improved management decision making capability (4) greater ease of determining relevant costs (5) reduced nonvalue added costs.

• Korean Journal of Child Studies
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• v.26 no.2
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• pp.75-89
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• 2005

The subjects of this study were 172 5-year-old children enrolled in a kindergarten located in Daejeon city. Instruments were the K-ABC(1997) to test children's intelligence and the TTCT(1999) to test children's creativity, Data were analyzed by Pearson's R and Canonical R. Results indicated that the relation between K-ABC IQ and TTCT CQ was statistically significant(r=.292, p<.000). This result is at variance with threshold theory where the correlation between intelligence and creativity is predicted to be high for children with IQ below 120, but above that level, the correlations are predicted to be much lower. A significant canonical function(Wilk's =.760, p<.000) was found between the sequential processing scale, achievement scale of K-ABC and elaborateness, resistance to premature closure of TTCT.

• Journal of Digital Convergence
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• v.14 no.5
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• pp.485-491
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• 2016

The purpose of this study was to compare of the fall risk factors for elderly in the city. 62 people aged 65 years or older were classified as fallers and nonfallers based on experience of their falls in the previous year. By comparing the difference between the groups via evaluations of general characteristics, health related behavior and chronic disease, balance-related psychological (K-ABC) and physical measurement (BBS), depression (SGDS), and the correlations between the significant differences in variables were identified. According to the results, K-ABC, BBS, and SGDS are statistically significant differences between fallers and nonfallers (P<0.05). Also it has positive correlations between BBS and K-ABC (r=0.499) whereas negative correlation between K-ABC and SGDS(r=-0.472).