• The Journal of Internal Korean Medicine
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• v.17 no.1
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• pp.265-277
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• 1996

Jwagyuyeum and Woogyuyeum, being known to reinforce Kidney-yin and -yang, were tested for the effects of on free radical generating enzyme and lipid peroxidation in rat's kidney. In vitro, levels of lipid peroxide in tissues of brain were proportionally decreased to concentration of extracts prepared from Jwagyuyeum and Woogyuyeum. They were much more decreased, when lipid peroxidation was induced with ferrous iron (Fe+2), In vivo, after both herbs were administered to the rat, levels of lipid peroxide in kidney were decreased only in Jwagyuyeum. The enzyme activities and the ratio of type conversion of xanthine oxidase were decreased only in Jwagyuyeum. But, We can't see special changes in Woogyuyeum. The enzyme activities of aldehyde oxidase was lowered in both herbs, especially Jwagyuyeum was much more done. These results suggest that Jwagyuyeum and Woogyuyeum decrease the activities of free radical generating enzymes such as xanthine oxidase and aldehyde oxidase which form lipid peroxide.

• The Journal of Internal Korean Medicine
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• v.16 no.1
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• pp.62-80
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• 1995

Jwagyuyeum and Woogyuyeum, being known to reinforce Kidney-yin and -yang, were tested for the effects of on free-radical generating enzyme and lipid peroxidation. In vitro, levels of lipid peroxide in tissues of liver were proportionally decreased to concentration of extracts prepared from Jwagyuyeum and Woogyuyeum. They were much more decreased, when lipid peroxidation was induced with ferrous iron (Fe+2). In vivo, after both herbs were administered to the rat, levels of lipid peroxide in liver were decreased only in Jwagyuyeum. And, enzyme activities of xanthine oxidase and aldehyde oxidase in liver were not changed. It was guessed that Jwagyuyeum and Woogyuyeum inhibited lipid peroxidation directly, or acted on free radical resolving enzymes which decrease lipid peroxide. Consequently both herbs, particularly in Jwagyuyeum might delay aging.

• The Journal of Korean Medicine
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• v.17 no.1 s.31
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• pp.21-36
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• 1996

This study was undertaken to examine the effect of Jwagyuyeum and Woogyuycum, being known to reinforce Kidney-yin and yang, on the activities of endogenous antioxidant enzymes and the production of oxygen free radicals in the liver and kidney tissues, Alterations in enzyme activities were observed after in vivo treatment in rats, Jwagyuyeum and Woogyuyeum caused a significant increase in the activities of superoxide dismutase(SOD) and catajase Jwagyuyeum significantly increased the activity of glutathione peroxidase in both liver and kidney, but the enzyme activity was not significantly altered by Woogyuyeum. Treatment in vitro of Jwagyuyeum and Woogyuyeum decreased the production of oxygen free radicals in a dose-dependent fashion. These results suggest that Jwagyuyeum and Woogyuyeum stimulate the activities of antioxidant enzymes and inhibit directly the production of oxygen free radicals. These effects of both herbs may contribute to prevent the oxygen free radical-induced impairment of cell function.

• The Journal of Korean Medicine
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• v.16 no.2 s.30
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• pp.348-364
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• 1995

Jwagyuyeum and Woogyuyeum, being known to reinforce Kidney-yin and -yang, were tested for the effects of on free radical generating enzyme and lipid peroxidation in senile rat brain. In vitro, levels of lipid peroxide in tissues of brain were proportionally decreased to concentration of extracts prepared from Jwagyuyeum and Woogyuyeum. They were much more decreased, when lipid peroxidation was induced with ferrous iron ($Fe^{-2}$). In vivo, after both berbs were administered to the rat. levels of lipid peroxide in brain were decreased. especially it was much more decreased using Jwagyuyeum. Also, enzyme activities of xanthine oxidase and aldehyde oxidase in brain were decreased. The ratio of type conversion of the brain xanthine oxidase was lowered in both, especially Jwagyuyeum was much more done. These results suggest that Jwagyuyeum and Woogyuyeum decrease the activities of free radical generating enzymes such as xanthine oxidase and aldehyde oxidase which form lipid peroxide, Consequently both herbs might delay aging.