• Journal of Applied Biological Chemistry
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• 제48권4호
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• pp.161-166
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• 2005

Among 24 isolates of fluorescent Pseudomonads, 5 isolates named as LPT1, LPT2, LPT3, LPT4 and LPT5 were screened in vitro for their nematicidal activity against cyst forming nematode, Heterodera cajani causing patchiness, poor and stunting growth besides discoloration in Sesamum indicum. Second stage juveniles of H. cajani hatched from egg masses were collected from roots of host plant and subjected to fresh and heat-treated culture filtrate of isolates for 24 h. Mortality of H. cajani was recorded on the basis of parameters used for test organism bioassay. Among these isolates, Pseudomonas aeruginosa LPT5 caused maximum mortality towards second stage juvenile of H. cajani in vitro. Five isolates were used as seed coating for the management of cyst forming nematode H. cajani on sesame in green house condition. The strains LPT5 was better than the other strains in reducing the population of H. cajani both in vitro and in vivo. The reduction in cyst and juveniles population was found to be 49 and 60%, respectively when seeds were coated with strain LPT5. Among other strains, LPT4 was also found to inhibit the cyst and juveniles population 12 and 36% respectively. Increases in early vegetative plant growth parameters recorded in both in vitro and in vivo further revealed the significance of indigenous bacteria in comparison to introduced strain.

• 한국수산과학회지
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• 제39권4호
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• pp.326-332
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• 2006

To investigate the effects of environmental salinity on the expression of the genes for growth hormone (GH) and prolactin (PRL) in the pituitary, and their receptors (GHR, PRLR) In the kidney, intestine, and gills in teleosts, we acclimated juvenile olive flounders (Paralichthys olivaceus) to different salinities (5, 15, 25, or 32 psu) for 3 days and examined their mRNA levels using the reverse transcription-polymerase chain reaction (RT-PCR). In the fish adapted to low salinity, the PRL mRNA levels in the pituitary were elevated dramatically, whereas the GH mRNA levels did not differ significantly. PRLR mRNA increased significantly in fish exposed to low salinity, whereas GHR mRNA levels did not differ. These results suggest that PRL is an important hormone for flounders that are acclimated to brackish water and it may control ion homeostasis with PRLR in the osmoregulatory organs.

• Fisheries and Aquatic Sciences
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• 제18권4호
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• pp.359-365
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• 2015

We investigated the effects of dietary lipid sources on growth and fatty acid composition of juvenile Chinese longsnout catfish. Triplicate groups of fish (initial average weight, 3.8 g) were fed four diets containing either fish oil (FO), soybean oil (SO), linseed oil (LO) and lauric acid (LA) for 10 weeks. There were no differences among the groups in body weight, feed intake, feed efficiency, protein efficiency ratio, and body proximate composition of fish fed the diets containing different lipid sources (P > 0.05) during the study. However, fatty acids compositions of the whole body were influenced by dietary lipid source. Fish fed the SO diet had high concentration of linoleic acid, whereas those of fish fed the LO diet were rich in linolenic acid and arachidonic acid. Fish fed the FO diet had significantly (P < 0.05) higher levels of monounsaturated fatty acids such as 18:1n-9 and 20:1n-9 than those of fish fed the SO and LO diets. Eicosapentaenoic acid (EPA; 20:5n-3) and docosahexaenoic acid (DHA; 22:6n-3) composition of body were not influenced by dietary lipid source. The results suggest that each of FO, SO, LO or LA can be used as a lipid source in the diets of Chinese longsnout catfish without any negative effects on growth and feed utilization and these data demonstrate the potential impact which dietary fat composition can change the body fatty acid profile.

• 수산해양교육연구
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• 제28권4호
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• pp.1098-1106
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• 2016

To study the influence of light intensity on blind-side hypermelanosis of starry flounder, Platichthys stellatus, we compared growth, glucose, cortisol, ratio of malpigmented blind-side area, ambicolored fish ratio and expression of mch mRNA for 60 days in 0 lux(darkness) and 20 lux(low light density). The test was done in duplicate at 50 fish/tank with the selected ordinary juvenile flounder. The rearing was performed in FRP aquarium tanks ($H100cm{\times}L100cm{\times}W100cm$). Growth was higher in the 0 lux. The ratio of malpigmented blind-side area and ambicolored fish ratio were significantly higher at the 0 lux than at the 20 lux. The result suggests that 20 lux could inhibit the hypermelanosis. The expression level of MCH 1, 2 mRNA was significantly lower in 0 lux, indicating that MCH 1, 2 and light intensity are related to blind-side hypermelanosis of starry flounder.

• Fisheries and Aquatic Sciences
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• 제26권8호
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• pp.461-469
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• 2023

The objective of this study was to evaluate how the tank colours may change the effects of extreme temperature on the survival, growth, and quality of juvenile golden trevally (Gnathanodon speciosus). The experiment was set up with fifteen treatments of five tank colours (blue, red, yellow, grey, and white) and three temperatures (30℃, 32℃, 34℃) with three replications. Fish performance was assessed for four weeks. The results showed that tank colours and elevated temperatures affected the quality of golden trevally juveniles. The survival and growth rate of fish tend to decrease gradually, but the deformation rate of fish tended to increase in the order of tank colours: red, yellow > grey, blue, and white. The growth and survival rate of fish gradually decreased when the rearing temperature increased from 30℃ to 34℃ and this effect was independent of tank colors. Importantly, the deformation rate increased under elevated temperature, particularly in blue and white tanks with potential long-term effects. It is, therefore, not recommended to use blue and white tanks for rearing the golden trevally juveniles, particularly during extremely high temperatures from heatwave events.

• 한국수산과학회지
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• 제33권5호
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• pp.463-468
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• 2000

첨가제로서 K. fragilis, C. utilis 및 맥주효모의 효능을 조사하기 위하여, 어분함량이 다른 배합사료에 각각의 효모를 $3{\%}$씩 첨가하여 7주간 3반복으로 사육 실험하였다. 생존율은 사료의 어분함량 및 첨가 효모종류에 영향을 받지 않았다 (P > 0.05). 효모를 첨가하지 않는 대조구의 증체율은 어분함량에 영향을 받지 않았다(P>0.05). 어분 $55{\%}$ 사료에서 K. fragilis 및 맥주효모 첨가구는 대조구와 유의차 없이 (P < 0.05) 양호하였으나, C. utilis 첨가구는 대조구보다 낮은 값을 보였다 (P < 0.05). 어분 $45{\%}$ 사료에서는 맥주효모 첨가구는 대조구와 비슷한 값을 보였으나, K. fragilis 및 C. utilis 첨가구는 대조구보다 낮은 값을 보였다 (P < 0.05), 사료효율 및 단백질효율은 대조구와 맥주효모 첨가구의 경우 어분함량에 영향을 받지 않았고 (P > 0.05), 어분 $55{\%}$ 사료에서는 효모 첨가종류에 따른 차이는 없었으나, 어분 $45{\%}$ 사료에서는 K. fragilis 및 C. utilis 첨가구가 대조구보다 유의하게 낮은 값을 보였다(P < 0.05). 간 중량지수는 어분함량에는 영향을 받지 않았지만, 첨가효모의 종류에 영향을 받아 두 어분 함량 모두에서 C. utilis 첨가구에서 가장 낮은 값을 보였다 (P < 0.05). 간의 수분, 단백질 및 지질 함량도 사료의 어분 함량에는 유의적인 차이는 없었으나 (P > 0.05), 첨가된 효모의 종류에 따라서는 차이를 보였는데, C. utilis 첨가구는 타 실험구보다 높은 수분과 단백질 그리고 낮은 지질함량을 보였다 (P < 0.05). Hb, Ht, RBC, 혈장 글루코오스, 혈장 단백질 함량은 모든 실험구간에서 유의차가 없었다 (P > 0.05). 이상의 결과로부터, 경제적인 측면에서 고려해 볼 때, 본 실험에서 사용된 사료 조성비에는 조피볼락 성장개선을 위한 효모첨가는 불필요한 것으로 판단된다.

• 한국해양학회지:바다
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• 제19권1호
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• pp.35-40
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• 2014

2011년 2월부터 5월까지 경남 남해군 연안에서 소형 Beam trawl에 채집된 문치가자미 치어 이석을 관찰 분석하여 부화시기와 초기성장을 추정하였다. 문치가자미 치어는 2011년 2월에서 5월까지 총 175개체가 채집되었며, 체장-체중 관계식은 $W=9.76^*10^{-8}L^{4.39}$ ($r^2=0.93$)를, 체장-이석경 관계식은 L=0.0795R+0.2249 ($r^2=0.92$)로 추정되었다. 체장 성장은 $L_t=135{\exp}\{-{\exp}[0.0103(t-116)]\}$ ($r^2=0.83$)로 Gompertz 성장식이 가장 유의하게 회귀되었다. 이석 일령으로부터 추정한 주 산란시기는 12월에서 1월인 것으로 추정되었다.

• Reproductive and Developmental Biology
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• 제34권2호
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• pp.81-88
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• 2010

Our objective of current study was to investigate the development of bone and heart in association with diabetes mellitus (DM). DM was induced by administering an intraperitoneal injection of streptozotocin (STZ; 60 mg/kg) to 4-week-old Sprague-Dawley rats. Body weight and blood glucose were monitored, and rats were sacrificed after 2 or 5 weeks. The left ventricle (LV), including the interventricular septum, was weighed, and body weight and tibial bone length were assessed. Young diabetic rats showed reduced growth in terms of tibial length and body weight compared to controls. Moreover, diabetic males showed more significant growth suppression and reduced LV size than diabetic females. Morphometric analysis of tibiae from diabetic rats revealed suppressed bone growth at 2 and 5 weeks, with no difference between genders. STZ-induced diabetes decreased bone growth and retarded pre-pubertal heart development. As a result, diabetes may increase cardiovascular risk factors and lead to eventual heart failure. Therefore, new therapeutic approaches are required for diabetic children exhibiting growth retardation. Heart growth factor, exercise, and cardiopulmonary physical therapy may be required to promote heart development and physiological function.

• 한국미생물·생명공학회지
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• 제29권2호
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• pp.104-109
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• 2001

공생 박테리아 Photorhabdus sp. strain TF 균체량이 증가함에 따라서 병원성 선충 Heterorhabditis bacteriophora의 Infective Juveniles의 생산이 증가되었다. 이 공생 박테리아의 성장 최적 조건은 각각 배양 온도 3$0^{\circ}C$ 그리고 초기 배지 pH 5.5부터 7.3 사이에서 얻어졌다. 통기 상태에서 박테리아 균체의 생산과 성장이 촉진됨이 밝혀졌다. Photorhabdus sp. strain TF 박테리아의 성장 중 생산된 색소와 배양액의 pH의 변화는 박테리아의 성장 정도 나타내어 액체배양에서 병원성 선충 Heterorhabditis bacteriophora의 접종시기로 표시로서 이용할 수 있을 것이다.

• 한국양식학회지
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• 제19권4호
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• pp.247-253
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• 2006

본 실험은 치어기 넙치에 잇어서 ${\beta}-1,3$ 글루칸을 사료내 첨가시 성장, 비특이적 면역반응 및 질병저항성에 미치는 영향을 조사하기 위하여 사료내 ${\beta}-1,3$ 글루칸을 수준별 첨가하여 실시였다. 실험어는 평균무게 3.2 g인 넙치 치어를 사용하였으며, 기초사료에 ${\beta}-1,3$ 글루칸을 대조구, ${\beta}-1,3$ 글루칸 0.01, 0.025%, 0.05% 및 0.1%를 각각 첨가하여 총 5개 실험구로 3반복배치하여 6주간 실시하였다. 총 6주간의 성장 실험결과, 면역증강물질인 ${\beta}-1,3$ 글루칸의 첨가에 따르는 사료효율과 단백질전환효율에 있어서 ${\beta}-1,3$ 글루칸 0.1%를 공급한 사료구가 대조구와 다른처리구에 비하여 유의하게 높은 값을 나타냈다(P<0.05). 증체율과 일간성장율에 있어서는 ${\beta}-1,3$ 글루칸 0.1%를 공급한 사료구가 대조구, ${\beta}-1,3$ 글루칸 0.01%, 0.025%를 첨가한 구보다 유의하게 높은 값을 나타내었지만 ${\beta}-1,3$ 글루칸 0.05%구와는 유의한 차이가 없었다. 비만도에 있어서는 ${\beta}-1,3$ 글루칸 0.05%와 0.1%를 첨가한 구가 대조구와 첨가구보다는 유의적으로 높았다. 헤마토크리트치는 ${\beta}-1,3$ 글루칸 0.05%와 0.1%첨가한 실험구가 대조구와 ${\beta}-1,3$ 글루칸을 0.01%와 0.025%를 첨가한 실험구에 비하여 유의하게 높은 값을 나타내었다(P<0.05). 혈청내 GOT에 있어서 ${\beta}-1,3$ 글루칸 0.05%와 0.1%를 첨가한 실험구가 대조구, ${\beta}-1,3$ 글루칸 0.01%와 0.025%를 첨가한 실험구보다 유의하게 낮은 값을 나타내었다(P<0.05). 비특이적 면역반응 결과에 있어서는 ${\beta}-1,3$ 글루칸을 0.05%와 0.1%를 첨가한 실험구가 혈청의 lysozyme 활성 및 두신 phagocyte의 chemiluminescent(CL) 반응에서 대조구, ${\beta}-1,3$ 글루칸 0.01%와 0.025%를 첨가한 실험구보다 유의하게 높은 값을 나타내었으나, 보체대체활성의 경로에 있어서는 전실험구간의 유의한 차이를 보이지 않았다. 공격 실험 결과에서는 ${\beta}-1,3$ 글루칸을 첨가한 실험구가 대조구에 비하여 초기폐사율이 낮음을 확인할수 있었으며, 상기 결과를 토대로, 넙치 치어의 경우 ${\beta}-1,3$ 글루칸을 0.05% 이상 0.1% 미만을 사료에 첨가하는 것이 성장, 사료효율 증진, 항산화능 및 질병저항성에 가장 좋은 효과를 나타낼 수 있을 것을 사료된다.