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Diversity of Epiphytic and Acid-tolerant Epiphytic Bacterial Communities on Plant Leaves

  • Joung Pil-Mun;Shin Kwang-Soo;Lim Jong-Soon;Park Seong Joo
    • Proceedings of the Microbiological Society of Korea Conference
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    • 2002.10a
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    • pp.100-105
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    • 2002
  • The diversity of epiphytic bacterial communities on deciduous oak tree (Quercus dentate Thunb.) leaves was examined both in the natural forest area with a clean air and in the industrial estate to assess effects of acidic deposition to the phyllosphere using 16S rDNA sequence data. In addition, acid-tolerant epiphytic bacterial communities were compared. A total of 78 epiphytic and 444 acid-tolerant clones were obtained from clone libraries, resulting in 20 and 17 phylotypes by analysis of restriction fragment length polymorphism (RFLP) for PCR-amplified 16S rDNA products. A low bacterial diversity in both areas was found. As tree leaves grow older, bacterial diversities were slightly increased in the level of subphylum. The community structure of epiphytic bacteria in both areas in April consisted of only two subphyla, $\beta-and\;\gamma-Proteobacteria$. In August two additional subphyla in both areas were found, but the composition was a little different, Acidobacteria and Cytophaga-Flexibacter-Bacteroids (CFB) group in the industrial estate and a -Proteobacteria and CFB group in the natural area, respectively. Acidobacteria could be an indicator of epiphytic bacteria for acidic deposition on plant leaves, whereas a -Proteobacteria be one of epiphytic bacteria that naturally survive on leaves that are not affected by acidic deposition. The acid-tolerant bacterial communities in April were composed of two subphyla, $\gamma-Proteobacteria$ and Low G+C gram-positive bacteria in both areas, and in August a-Proteobacteria was added to the community just in the natural forest area. The direct influence of acidic deposition on the acid-tolerant bacterial phylogenetic composition could not be detected in higher taxonomic levels such as subphylum, but at narrower or finer levels it could be observed by a detection of Xanthomonadales group of $\gamma-Proteobacteria$ just in the industrial estate.

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Dynamic Analysis of a Deployable Space Structure Using Passive Deployment Mechanism (수동형 전개힌지를 이용한 전개형 우주 구조물의 전개 동특성 해석)

  • Choi, Young-Jun;Oh, Hyun-Ung;Choi, Yong-Hoon;Lee, Kyung-Joo
    • Journal of the Korea Institute of Military Science and Technology
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    • v.11 no.3
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    • pp.161-168
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    • 2008
  • The deployable space structure is necessary to minimize the satellite volume and launch cost. For the deployment, passive deployment mechanism has widely been used to attenuate a latch shock induced when the structure is just fully deployed. To reduce the latch shock, viscous damper is applied to the passive deployment mechanism and it can control the deployment speed of the structure. In this paper, dynamic analysis of the deployable space structure using the passive deployment mechanism with the viscous damper has been performed. The viscous damping values have been optimized through numerical simulation. The satellite's attitude influenced by pyro activation for the release of the structure has also been investigated.

Study of Waterhammer Suppression Technique due to Valve Closing on Water Supply Pipeline (송수관로 밸브폐쇄에 따른 수충격현상 완화기법 연구)

  • Park, Jong-Ho;Park, Han-Yung
    • The KSFM Journal of Fluid Machinery
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    • v.14 no.6
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    • pp.11-17
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    • 2011
  • The cause of waterhammer phenomenon due to valve closing which is installed on pipeline is clarified in this study. Also if waterhammer phenomenon occurs on simple pipeline, expensive facilities like pressure relief valve is adapted to protect pipeline from waterhammer so far. But this study shows that waterhammer phenomenon can be suppressed by just simple modification of valve control sequence, and this technique is verified by simulation and site experiment.

an RFID based Collaborative Production Support System for Automotive Module Parts (RFID기반 모듈화 부품 생산지원시스템에 관한 연구)

  • Kim, San-Lack;Ahn, Geon-Tae
    • Proceedings of the Korean Society of Computer Information Conference
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    • 2008.06a
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    • pp.291-295
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    • 2008
  • 오늘날 정보기술(IT)은 다양한 산업분야 융화되어 기존 산업의 경쟁력을 강화하고 생산성 및 품질 향상의 견인차 역할을 수행하고 있다. 특히 생산 및 물류 분야에서 IT 융합을 통한 새로운 시너지 창출을 시도하고 있는 것이 RFID 기술이라 할 수 있다. 이미 정부 주도의 시범사업이 끝났으며, 2008년 공공기간 확산사업이 성공적으로 마무리되면, 본격적으로 산업분야 확산이 기대되고 있다. 본 논문에서 자동차 산업에서의 RFID 응용 모델로서 RFID 기반 지능형 직서열 생산시스템의 설계 및 구현에 대하여 기술한다. 직서열 시스템은 완성차 생산라인의 조립시간과 순서에 맞춰 모듈과 부품을 공급하는 협업 생산시스템이다. 자동차 조달물류 프로세스 혁신을 위하여 RFID 기술을 도입함으로써 협력기업간 정보 교환을 원활하게 지원하며 실시간 추적관리 체제의 구현을 이루고자 한다. 본 논문에서 제안하는 시스템은실시간 재고 파악 및 생산물량 예측이 가능하며, 이기종 부품 장착에 의한 조립불량률을 현저히 줄일 수 있는 장점이 있다.

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Chloroplast-type Ferredoxin Involved in Reactivation of Catechol 2,3-Dioxygenase from Pseudomonas sp.S-47

  • Park, Dong-Woo;Chae, Jong-Chan;Kim, Young-Soo;Iida, Toshiya;Kudo, Toshiaki;Kim, Chi-Kyung
    • BMB Reports
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    • v.35 no.4
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    • pp.432-436
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    • 2002
  • Pseudomonas sp. S-47 is capable of degrading catechol and 4-chlorocatechol via the meta-cleavage pathway. XyITE products catalyze the dioxygenation of the aromatics. The sylT of the strain S-47 is located just upstream of the xylE gene. XylT of the strain S-47 is located just upstream of the xylE gene. XyIT is typical chloroplast-type ferredoxin, which is characterized by 4 cystein residues that are located at positions 41, 46, 49, and 81. The chloroplast-type ferredoxin of Pseudomonas sp. S-47 exhibited a 98% identity with that of P. putida mt-2(TOL plasmid) in the amino acid sequence, but only about a 40 to 60% identity with the corresponding enzymes from other organisms. We constructed two recombinant plasmids (pRES1 containing xylTE and pRES101 containing xylE without xylT) in order to examine the function of XyIT for the reactivation of the catechol 2,3-dioxygenase (XyIE) that is oxidized with hydrogen peroxide was recovered in the catechol 2,3-dioxygenase (C23O) activity about 4 mimutes after incubation, but the pRES101 showed no recovery. That means that the typical chloroplast-type ferredoxin (XyIT) of Pseudomonas sp. S-47 is involved in the reactivation of the oxidized C23O in the dioxygenolytic cleavage of aromatic compounds.

A CONSTRUCTION METHOD OF MULTIPLE CONTROL SYSTEMS USING PARTIAL KNOWLEDGE UPON SYSTEM DYNAMICS

  • Yoshisara, Ikuo;Indaba, Masaaki;Aoyama, Tomoo;Yasunaga, Moritoshi
    • 제어로봇시스템학회:학술대회논문집
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    • 1999.10a
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    • pp.73-78
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    • 1999
  • This paper presents an effective construction method of adaptive multiple control systems utilizing some knowledge upon the plants. The adaptive multiple control system operates plants un-der widely changing environmental conditions. The adaptive multiple control system is composed of a family of candidate controllers together with a supervisor. The system does not require any identification schemes of environmental conditions. Monitoring outputs of the plant, the supervisor switches from one candidate controller to another, The basic ideas of adaptation are as follows: (1)each candidate controller is prepared for each environmental condition in advance; (2)the supervise. applies a sequence of speculative controls to the plant with candidate controllers just after the start of control or just after the detection of a change in the environmental condition. Each candidate controller can keep the system stable during one-step period of the speculative control and the most appropriate candidate controller for the environmental condition to which the system is exposed can be selected before the last trial of speculative control step comes to an end. We proposed a construction method of adaptive multiple control system without any knowledge of plant dynamics and applied the method to a cart-pole balancing problem and a vehicle anti skid braking system. In real applications, as we can often easily obtain a piece of knowledge upon plant dynamics beforehand, we intend to extend the method such that multiple control systems can be efficiently designed using the knowledge. We apply the new idea to the cart-pole balancing problem with variable length of the pole. The simulation experiments lead us to the conclusion that the new attempt can reduce the manpower to design the candidate controllers for adaptive multiple control systems.

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Molecular Cloning of the Gene in Schizosaccharomyces pombe Related to the CDC3 Gene in Saccharomyces cerevisiae (Saccharomyces cerevisiae의 CDC3 유전자와 유사한 Schizosaccharomyces pombe 유전자의 클로닝)

  • 김형배
    • Korean Journal of Microbiology
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    • v.31 no.3
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    • pp.197-202
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    • 1993
  • The budding yeast S. cerevisiae contains 10-nm filament ring that lies just inside the plasma memhrane in the region of the mother-bud neck. It is possihle that CDC3. CDCIO, CDCII. CDCI2 genes encode the filaments. Recently it has been shown that the CDC3 and CDCI2 gene products arc localized to [he vicinity of the neck lilaments by immunolluorescence. However. the role of the lilament ring is not clear. In order to find out the role of filament ring. I have tried to clone the similar gene in S. pomhe to the CDC3 in S. cerevisiae. Genomic library was constructed by use of $\lambda$gtll expression vector and screened with CDC3 antibodies. From sequencing data, there were more than two introns in the newly cloned gene. There was 62% homology between the part of the predicted amino acid sequence of cloned gene and CDC3 amino acid sequence.

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Integrate Processing Scheme of Flow Control Language (흐름 제어 언어의 통합 처리)

  • Kim, Tae-Wan;Chang, Chun-Hyon
    • The KIPS Transactions:PartD
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    • v.11D no.2
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    • pp.415-422
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    • 2004
  • Automation systems improve the productivity of works which relate to product design, facilities management, fault processing and quality evaluation. In these systems, the description language for monitoring and control process is called new control language. These are five flow control languages : IL, ST, FBD, SFC and LD. IL and ST are based on left form. FBD, SFC and LD are based on graphic form. Generally, a software which monitors and controls a system is allowed to use just one flow control language. It is impossible to use more than two languages for simulation in the same system environment. In this paper, we analyzed the characteristics of flow control languages and the process of programming in the legacy system. In addition, for the Integrated processing of languages, we propose Extended ST based on the high-level ST language. Based on this research, we implement a graphical language editor and EST-IL convertor. The graphical language editor makes sequence rules, and converts graphical language into EST. EST-IL convertor has a function to convert EST into IL which is similar to assembly language. As the result of this paper, we present a scheme which integrates all the flow control language processing based on IL.

Clinical Factors Associated with the Non-Operative Airway Management of Patients with Robin Sequence

  • Albino, Frank P.;Wood, Benjamin C.;Han, Kevin D.;Yi, Sojung;Seruya, Mitchel;Rogers, Gary F.;Oh, Albert K.
    • Archives of Plastic Surgery
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    • v.43 no.6
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    • pp.506-511
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    • 2016
  • Background The indications for surgical airway management in patients with Robin sequence (RS) and severe airway obstruction have not been well defined. While certain patients with RS clearly require surgical airway intervention and other patients just as clearly can be managed with conservative measures alone, a significant proportion of patients with RS present with a more confusing and ambiguous clinical course. The purpose of this study was to describe the clinical features and objective findings of patients with RS whose airways were successfully managed without surgical intervention. Methods The authors retrospectively reviewed the medical charts of infants with RS evaluated for potential surgical airway management between 1994 and 2014. Patients who were successfully managed without surgical intervention were included. Patient demographics, nutritional and respiratory status, laboratory values, and polysomnography (PSG) findings were recorded. Results Thirty-two infants met the inclusion criteria. The average hospital stay was 16.8 days (range, 5-70 days). Oxygen desaturation (<70% by pulse oximetry) occurred in the majority of patients and was managed with temporary oxygen supplementation by nasal cannula (59%) or endotracheal intubation (31%). Seventy-five percent of patients required a temporary nasogastric tube for nutritional support, and a gastrostomy tube placed was placed in 9%. All patients continued to gain weight following the implementation of these conservative measures. PSG data (n=26) demonstrated mild to moderate obstruction, a mean apneahypopnea index (AHI) of $19.2{\pm}5.3events/hour$, and an oxygen saturation level <90% during only 4% of the total sleep time. Conclusions Nonsurgical airway management was successful in patients who demonstrated consistent weight gain and mild to moderate obstruction on PSG, with a mean AHI of <20 events/hour.

A Case Study on Capacitated Lot-sizing and Scheduling in a Paper Remanufacturing System (제지 재제조 시스템에서의 자원제약을 고려한 로트 크기 결정 및 일정 계획에 대한 사례연구)

  • Kim, Hyeok-Chol;Doh, Hyoung-Ho;Yu, Jae-Min;Kim, Jun-Gyu;Lee, Dong-Ho
    • Journal of Korean Society of Industrial and Systems Engineering
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    • v.35 no.3
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    • pp.77-86
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    • 2012
  • We consider the capacitated lot-sizing and scheduling problem for a paper remanufacturing system that produces several types of corrugated cardboards. The problem is to determine the lot sizes as well as the sequence of lots for the objective of minimizing the sum of setup and inventory holding costs while satisfying the demand and the machine capacity over a given planning horizon. In particular, the paper remanufacturing system has sequence-dependent setup costs that depend on the type of product just completed and on the product to be processed. Also, the setup state at one period can be carried over to the next period. An integer programming model is presented to describe the problem. Due to the complexity of the problem, we modify the existing two-stage heuristics in which an initial solution is obtained and then it is improved using a multi-pass interchange method. To show the performances of the heuristics, computational experiments were done using the real data, and a significant amount of improvement is reported.