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Chloroplast-type Ferredoxin Involved in Reactivation of Catechol 2,3-Dioxygenase from Pseudomonas sp.S-47

  • Park, Dong-Woo (Department of Microbiology and Biotechnology, and Research Institute for Genetic Engineering, Chungbuk National University) ;
  • Chae, Jong-Chan (Department of Microbiology and Biotechnology, and Research Institute for Genetic Engineering, Chungbuk National University) ;
  • Kim, Young-Soo (Department of Pharmacy, Chungbuk National University) ;
  • Iida, Toshiya (Laboratory of Microbiology, The Institute of Physical and Chemical Research (RIKEN)) ;
  • Kudo, Toshiaki (Laboratory of Microbiology, The Institute of Physical and Chemical Research (RIKEN)) ;
  • Kim, Chi-Kyung (Department of Microbiology and Biotechnology, and Research Institute for Genetic Engineering, Chungbuk National University)
  • Published : 2002.07.31
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Abstract

Pseudomonas sp. S-47 is capable of degrading catechol and 4-chlorocatechol via the meta-cleavage pathway. XyITE products catalyze the dioxygenation of the aromatics. The sylT of the strain S-47 is located just upstream of the xylE gene. XylT of the strain S-47 is located just upstream of the xylE gene. XyIT is typical chloroplast-type ferredoxin, which is characterized by 4 cystein residues that are located at positions 41, 46, 49, and 81. The chloroplast-type ferredoxin of Pseudomonas sp. S-47 exhibited a 98% identity with that of P. putida mt-2(TOL plasmid) in the amino acid sequence, but only about a 40 to 60% identity with the corresponding enzymes from other organisms. We constructed two recombinant plasmids (pRES1 containing xylTE and pRES101 containing xylE without xylT) in order to examine the function of XyIT for the reactivation of the catechol 2,3-dioxygenase (XyIE) that is oxidized with hydrogen peroxide was recovered in the catechol 2,3-dioxygenase (C23O) activity about 4 mimutes after incubation, but the pRES101 showed no recovery. That means that the typical chloroplast-type ferredoxin (XyIT) of Pseudomonas sp. S-47 is involved in the reactivation of the oxidized C23O in the dioxygenolytic cleavage of aromatic compounds.

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References

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