• 한국식품영양과학회지
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• 제27권6호
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• pp.1148-1151
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• 1998

We investigated the inhibition effects of teeth decalcification and glucosyltransferase(GTase) activity on the Juniperus rigida S. et Z. Teeth decalcifications by Streptococcus mutans were respectively inhibited to 70.13, 74.93% on methanol and n hexane fractions. In the inhibition test of GTase activities by solvent fractions of J. rigida, they were respectively inhibited to 86.6, 89.5% to a similar degree. And in the result to identify GTase produced by S. mutans with SDS PAGE, the band near 65KD estimated as GTase did not show in the lanes of methanol, n hexane and chloroform fractions.

• Journal of the Korean Wood Science and Technology
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• 제32권1호
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• pp.59-66
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• 2004

노간주나무 잎을 채취하여 건조시킨 후 분말로 제조하여 1.5 kg을 아세톤-물(7:3, v/v)로 추출하고 헥산, 메틸렌클로라이드, 에틸아세테이트 및 수용성으로 분획하여 동결 건조시켰다. 그 중에서 에틸아세테이트용성과 수용성 분획을 Sephadex LH-20으로 충진한 칼럼에서 메탄올과 에탄올-헥산 혼합액을 용리용매로 사용하여 칼럼크로마토그래피를 실시하였다. 단리된 화합물들은 TLC로 확인한 후 NMR스펙트럼을 사용하여 정확한 구조를 규명하였고 FAB 및 EI-MS로써 분자량을 측정하였다. 주로 많은 양의 (+)-catechin, quercetin-3-O-α-L-rhamnopyranoside와 isoconiferin이 분리되었으며, 소량의 umbelliferone과 quercetin-3-O- β-D-rutinoside도 분리되었다. 각 분획물들과 단리된 화합물들은 DPPH 라디칼 소거법을 이용하여 항산화실험을 실시하였으며, 분획물중에는 에틸아세테이트용성이 그리고 화합물중에는 (+)-catechin과 quercetin-3-O-α-L-rhamnopyranoside이 우수한 항산화 효능이 있는 것으로 나타났다.

• 한국균학회지
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• 제43권2호
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• pp.133-136
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• 2015

We investigated endophytic fungi diversity of two Pinaceae species (Pinus densiflora, Pinus rigida) and one Cupressaceae species (Juniperus rigida) on Mt. Seodae, Korea. In total, 34 isolates were obtained from 19 host plants and identified according to internal transcribed spacer (ITS) region. As a result, they were placed in 13 taxa. Among them, 52.9% belonged to Leotiomycetes, 32.3% belonged to Sordariomycetes, 5.8% belonged to Agaricomycetes, and 3.0% for isolates belonged to either Eurotiomycetes, Dothideomycetes or Ustilaginomycetes. In particular, Lophodermium growing in pine would be an especially important instance of biodiversity for endophytes, suggesting that further study examining its ecological function in the environment is critical.

• Applied Biological Chemistry
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• 제41권5호
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• pp.395-398
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• 1998

생약에서 충치균에 대한 항균성 물질을 개발하기 위하여 수종의 야생생약을 수집하여 항균활성을 측정해 보았다. 그 중에서 충치균에 강한 항균활성을 나타낸 두송실(Juniperus rigida S. et Z.)을 methanol로서 추출하고 극성이 다른 수종의 용매로서 분획하여, 충치균에 대한 항균활성과 유기산의 생성억제효과를 측정해 본 결과, n-hexane, chloroform 분획에서 활성이 나타났다. 충치균에 의하여 생성된 유기산은 주로 lactic acid와 citric acid로 전체의 $80{\sim}90%$를 차지하였는데 두송실의 methanol추출물과 n-hexane분획물을 1 mg/ml 처리한 시험군에서 각각 89.3, 90.8%의 유기산 생성 억제효과를 나타내었다.

• 한국균학회지
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• 제46권4호
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• pp.437-446
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• 2018

We investigated the biodiversity of endophytic fungi in Pinus densiflora and Junipreus rigida from Mt. Baekryeonsan and Mt. Johangsan in Korea. In total, 59 isolates were discovered from 26 host plants and identified using sequences from the rDNA internal transcribed spacer (ITS) region. Twenty taxa of endophytic fungi were identified in this study; 62% of isolates belonged to Leotiomycetes, 34% belonged to Sordariomyetes, and 4% belonged to Agaricomycetes. Results confirmed three previously unreported endophytic fungi in Korea: Phanerochaete concrescens, Creosphaeria sassafras, and Scopuloides rimosa. Community analysis showed that the fungal diversity patterns of the samples from Mt Baekryeonsan and Mt. Johangsan are similar to those from previous studies and varied depending upon host and locality.

• 생약학회지
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• 제41권3호
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• pp.216-220
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• 2010

Obesity is an important risk factor that significantly increases mortality and disease rates in the cardiovascular disease, diabetes, and various diseases. So far, the most powerful way to inhibit fat absorption is pancreatic lipase inhibitors. In this study, we investigated the anti-obesity effect of the extract of Juniperus rigida. Juniperus rigida extract (JRE) had a inhibitory effect on pancreatic lipase activity ($IC_{50}$=8.63 ${\mu}g$/ml). In in vivo oil-emulsion loading test, this extract also inhibited the intestinal fat absorption. In addition, we measured inhibitory effects of JRE on activity of phosphodiesterase (PDE) and hormone sensitive lipase (HSL) among the important enzymes associated with lipolysis. JRE strongly inhibited PDE activity ($IC_{50}$=4.56 ${\mu}g$/ml), whereas inhibitory effect on HSL activity was very weak compared with orlistat. As a result, JRE inhibited the absorption of fat by inhibiting the activity of pancreatic lipase and induced lipolysis through inhibition of PDE activity. Therefore, we suggest that Juniperus rigida may be a potential therapeutic agent improving obesity.

• 한국산림과학회지
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• 제73권1호
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• pp.9-13
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• 1986

Juniperus rigida의 경남(慶南)과 충북(忠北)의 두 산지종(産地種)에 대하여 핵형분석(核型分析)을 하였다. 염색체수(梁色體數)는 두 산지종(産地種) 공(共)히 2n=22이다. 두 산지종(産地種)에 있어 가장 공통적(共通的)인 특징(特徵)을 나타낸 염색체(染色體)는 두완(短腕)에 secondary constriction을 지닌 No.7 이고, 가장 특이적(特異的) 특징(特徵)을 한 염색체(染色體)는 secondary constriction을 지닌 경남산(慶南産)의 No.9와 충북산(忠北産)의 No.5 염색체(染色體)이다. 핵형식(核型式)은 다음과 같다. 경남산지종(慶南山地種) $$K(2n)=22=2A^m+2B^m+2C^m+2D^{sm}+2E^{st}+2F^m+2^{sc}G^m+2H^m+2^{sc}I^t+2J^{st}+2K^m$$ 충북산지종(忠北山地種) $$K(2n)=22=2A^m+2B^m+2C^m+2D^{st}+2^{sc}E^{sm}+2F^m+2^{sc}G^m+2H^m+2I^m+2J^{st}+2K^{sm}$$.

• 한국동물학회:학술대회논문집
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• 한국동물학회 1999년도 한국생물과학협회 학술발표대회
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• pp.294.2-294
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• 1999

No Abstract, See Full Text

• 동굴
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• 제56권
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• pp.15-38
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• 1998

Biomass and soil properties of plant communites was investigated in the limestone ares, Tanyang, Ch'ungbuk Province in Korea. Plants was classified in calcicoles and calcifuges within plant communities occurring in the limestone area. Biomass of plant populatins in the limestone area was measured that Stellaria aquatica was the lowest(0.58g/plant), Chrysanthemum boreale was the highest(8.87g/plant) and that Pennisetum alopecuroides, Miscanthus sinensis was 6.67g/plant, 5.76g/plant, respectively. Diameter of breast height among the tree layer communities was investigated that Pinus densiflora was the widest(20cm), Juniperus rigida was the narrowest(6cm) and that Quercus aliena, Populus ${\times}$ tomentiglandulosa was 10cm, 12cm, respectively. The high of density in the tree layer was found out Populus ${\times}$ tomentiglandulosa, Juniperus rigida, Quercus aliena, Pinus densiflora in this order. Dominant species in the study area were Pinus densiflora, Quercus aliena, Juniperus rigida, Quercus dentata in the tree layer, Lespedeza bicolor, Buxus microphylla, Rhus chinensis in the shrub layer, Humulus Japonicus, Erigeron canadensis, Xanthium strumarium, Oenothera odorata Persicaria hypropiper on the riverside, Pennisetum alopecuroides, Vicia amoena, Chrysanthemum boreale on the roadside. Soil properties of the limestone area was investigated around the protrusion of rock in the upper region, around the cave and underlayer accumulated by corrosion in the study area. Lime-chlorosis was observed in the upper region, underlayer and around the cave. It was observed that biota of kum-gul and dungbong-gul was Rhinolophus ferrumequinum korai, Epanerchodus kimi, Antrokoreana gracilipes, Diestrammena japonical. Dominant species were Humulus japonicus, Stellaria aquatica, Hydrocotyle maritima, Phragmites communis, Miscanthus sinensis around the inlet of a kum-gul. It was classified that plants in the study area was 34 order, 53 family, 135 species in all. Calcicoles were 11 order, 16 family, 18 species and calcufuges were 6 order, 8 family, 11 species of them all.

• 생명과학회지
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• 제23권12호
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• pp.1445-1453
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• 2013

사람의 피부는 자외선, 오염된 공기, 화학제품 등 환경에 끊임없이 노출된다. 특히 자외선은 피부에 다양한 형태로 영향을 주어 주름, 잔주름, 피부거침, 피부건조와 같은 현상을 발생시켜 피부노화를 유발시킨다. 미백효과를 확인하기 위하여 tyrosinase 저해활성을 측정한 결과 열수 추출물에서 49.4%, 에탄올 추출물에서 80.0%의 효과를 나타내어 열수 추출물에 비해 에탄올 추출물의 효과가 높게 나타났으며, 노간주나무 에탄올 추출물이 B16F10 흑세포종에 대하여 멜라닌 색소 생성 억제 및 tyrosinase, MITF, TRP-1, TRP-2의 억제 효과를 가지고 있는 것을 확인하였다. 이와 같은 결과로 미루어 보아 열수 추출물보다 에탄올 추출물이 더 높은 효과를 나타냄을 확인할 수 있었으며, 노간주나무 추출물의 미백효과를 이용하여 기능성 화장품 소재로서의 가능성을 확인할 수 있었다.