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Histopathological Study of Acute Toxicity of Ammonia on Common Carp Cyprinus carpio (잉어, Cyprinus carpio에 미치는 암모니아 급성독성의 조직병리학적 연구)

  • YANG Han Choon;CHUN Seh-Kyu
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.19 no.3
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    • pp.249-256
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    • 1986
  • The carp (Cyprinus carpio) used in the experiment were hatched in the spring this year and reared to 5.96g($4.84{\sim}6.55g$) in mean weight in a nursery pond at Daeyon fish farm, Pusan, Korea. The sample fish were exposed to different conditions of total ammonia (TA-N) concentrations 10, 20 and 30ppm and pH 6.5, 7.0, 7.5 and 8.0 at water temperatures 20, 25 and $30^{\circ}C$ for 24, 48 and 72 hours. After the procedure, the gill, liver and kidney of the fish were examined histopathologically. In this experiment, with the rise of water temperature, increase of pH and ammonia concentration, and the extension of exposure time the three organs showed the tendency of apparent abnormal changes such as hypertrophy and necrosis in their tissues. At $20^{\circ}C$ of water temperature gill tissue did not show any abnormality regardless of the change of pH at 10 ppm of ammonia concentration for 24 hours of exposure, but beyond the conditions given above, there occurred hypertrophy and the epithelium of gill lamellae was detached. The detach of gill lamellae epithelium initiated from the proximal part of the gill lamellae then gradually spread toward the uppermost tip. The heavier vacuolation of the liver was observed with the rise of water temperature and pH, and such morbid state in the liver was considered to be the result of edema in the liver tissue. The kidney showed no damage to the renal tubule epithelium at pH 6.5, but it was damaged at pH 8.0 when exposed to 30 ppm ammonia at $20^{\circ}C$ for 24 hours.

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Origin and Developing Process of Ark-shell Culture Industry in Korea (우리나라 꼬막양식업의 발상과 발달과정)

  • BAE Su-Whan
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.19 no.1
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    • pp.72-82
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    • 1986
  • There were three important species among the marine organisms which were cultured in the inter tidal zone in the old days, and an ark-shell was one of them. Exaiming old books and reports concerning fishery were made in order to reveal the history and industrial trend of ark-shell culture. Ark-shell was called by the different names in the old days "Komak" and "Sohap". The name of Komak was originated in the southern coast area in the 1,700s, and from the begining of 18 century Komak was used only. Ark-shell was produced in all southern and western coast areas, where a little of fresh water flows into the calm inner sea and the intertidal zone is well developed. It was well known that ark-shells shell as well as its flesh took the effect of a medicine. Its culturing method in Korea seems to be derived from China in the beginning of 19 century, it was cultured first at the southern area of Chollanam-do. It was considered as the side business, but after Korean war it is produced on the basis of cooperative work, now its production is quite increased (more than $5,000\fra{M}{T}$) due to the goverments development policy.

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BACTERIOLOGICAL STUDY ON CULTURED VEGETABLES (고등소채류에 대한 세균학적 연구)

  • CHANG Dong-Suck;KIM Young-Man;KIM Young-Goan
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.12 no.4
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    • pp.261-266
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    • 1979
  • This experiment was carried out to evaluate the sanitary quality of cultured vegetables and to check the removing rate of bacteria by treating methods such as washing with tap water or commercial detergent, or blanching. Samples collected from farm land located at Busan suburbs and markets were Fragaria chiloensis var. ananasa, Lycopersicum esculentum, Capsium longum, Cucumis sativus, Lactuca scariola var. sativa leaf of Perilla frutescens var, japonica, Oananthe stolonifera and Allium odoium. Fecal coliform MPN was ra god from less than 30 to 430,000 per 100 grams of samples examined while plate count was $7.2\times10^3\;to\;2.2\times10^7$ per gram. Usually contamination rate of fecal coliform of leaf vegetal)les was much higher than that of fruit vegetables. Removing rate of bacterial density of vegetables by washing three times with tap water was about $70\%$ in fruit vegetables, about $20\%$ in leaf vegetables but it was about $80\%$ in leaf vegetables by washing with a commercial detergent. Sirvival rate of viable cell count of leaf vegetables was less than $0.1\%$ after blanching for one minutes in boiling tap water, Composition of coliform was $18\%$ Escherichia coli group, $22\%$ Citrobacter freundii group and $60\%$ Klebsiella aerogenes group, among Escherichia coli, type I being $16\%$ in total. The coliform detection from BGLB gas positive tubes being planted with various vegetables was about $80\%$ in the result of IMViC reaction.

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A Study on the Preparation and Characteristics of Electrodeposited Lead Dioxide Electrodes (전착 이산화납전극의 제조 및 특성에 관한 연구)

  • Kim, Jae Kwan;Lee, Chung Young;Nam, Chong Woo
    • Applied Chemistry for Engineering
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    • v.4 no.2
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    • pp.273-283
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    • 1993
  • Addition of various acids or organic compounds to the electrolyte solution during the electrodeposition of $PbO_2$ on titanium madras substrate strongly affected performance of the deposited $PbO_2$ layer. Results of X-ray diffractometry ascertained that ${\beta}-PbO_2$ was deposited in acidic electrolyte. Among additives used in this experiment, $PbO_2$ with a high oxygen overvoltage was electrodeposited when sodium lauryl sulfate was added, and $PbO_2$ with a lower chlorine overvoltage was electrodeposited when polyethylene glycol was added to the electrolyte solution. The oxygen and chlorine overvoltage of $PbO_2$ was strongly dependent on the stirring provided during the electrodeposition experiment. It was observed by the SEM results that the $PbO_2$ grains deposited when stirring was not provided during the electrodeposition have larger than $PbO_2$ grains deposited by stirring. In the $PbO_2$ deposition under acidic electrolyte, the oxygen overvoltage increased with larger $PbO_2$ grains and the chlorine overvoltage decreased with smaller $PbO_2$ grains. The optimal current efficiency of $PbO_2$ in the presence of perchloric acid was observed at $Pb(NO_3)_2$ 560g/l, $65{\sim}70^{\circ}C$, and pH>1.

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A study on the development of 'Hodojook' (호도죽의 개발에 관한 연구)

  • June, Jung-Hee;Yoon, Jae-Young;Kim, Hee-Sup
    • Journal of the Korean Society of Food Culture
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    • v.13 no.5
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    • pp.509-518
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    • 1998
  • The purpose of this study was to develop korean traditional gruel 'Hodojook' as a easy-to cook product by modifying cooking method and its concentrations. Physical and sensory properties of 'Hodojook' with various levels of concentration were investigated using viskograph and sensory evaluation panel. The result showed that hydrated rice flour has higher maximum viscosity than instantly ground rice. Addition of walnut lowered maximum viscosity and gelatinization beginning temperature. But gelatinization pattern was not affected by the level of walnut and modified cooking method. Sensory characteristics on the roasted odor, roasted taste, oily odor, oily taste and viscosity were significantly increased as the walnut levels are increased. 'Hodojook' of' with the 1:1:6 ratio (rice flour walnut : water) was recommended.

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Influence of Glass Fiber Orientation on the Bi-directional GFRP Characteristics (직교이방향 GFRP 재료 특성에 미치는 유리 섬유방향의 영향)

  • Suh, Jung-Joo;Moon, Duk-Hong
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.21 no.1
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    • pp.75-81
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    • 1985
  • The tensile and dielctric strength of the epoxied resin with bi-directional woven glass fibers with a laminate of two layers(G-10) are studied, and the test parameter is the angle between fiber orientation and the tensile axis. The obtained results may be summaried as follows: 1) when the angle between fiber orientation and tensile axis was varied from 0$^{\circ}$ to 45$^{\circ}$ the yield and fracture stresses have a tendency to decrease with increase in the angle. Especially, the decrease rates in the yield and fracture stresses are changed remarkably in the range of 0$^{\circ}$ to 15$^{\circ}$. 2) The fracture strain has showed the maximum value when the angle between fiber orientation and tensile axis is 45$^{\circ}$, and showed the rapid rate of change from 15$^{\circ}$ to 45$^{\circ}$. 3) For the sample with same angle between fiber orientation and tensile axis the maximum dielectric strength under compressive stress is decreased with increase in tensile stress, when the compressive stress is increased as a parameter of tensile stress. 4) When the angle between fiber orientation and tensile axis is 45$^{\circ}$, the dielectric strength showed the worst value, as the mechanical strength did.

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Chemical Components of Hololeion maximowiczii Root and Change by Drying Methods (께묵뿌리의 화학성분과 건조방법에 따른 변화)

  • Choi, Dong-Yeon;Kim, Young-Hee;Park, Hoon;Yang, Cha-Bum
    • Korean Journal of Food Science and Technology
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    • v.25 no.5
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    • pp.421-425
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    • 1993
  • Hololeion maximowiczii root was analyzed for proximate composition, free sugar, fatty acids of crude lipid, amino acids and minerals after freeze dried (FD), hot air dried (HAD) and hot air dried after blanched (HADB), respectively. Proximate composition was little different among drying methods. Free sugar was abundant in order of sucrose $(28{\sim}29mg/g\;dw)$, fructose $(0.8{\sim}3mg/g\;dw)$, glucose and xylose (trace). Fructose was decreased in HADB. In fatty acid composition of crude lipids, linoleic acid was highest $(55{\sim}59%)$ and then palmitic and linolenic acid in abundance. These three fatty acids made $88{\sim}90%$ of total lipid, Low molecular fatty acids showed decreasing tendancy in HAD and HADB. Amino acid composition of hydrolyzed sample showed abundancy in order of arginine $(34{\sim}42mg/g)$, lysine, glutamic acid, glycine, aspartic acid, valine and histidine, while that of free amino acids was arginine, histidine, lysine, aspartic acid, serine, glutamic acid, isoleucine. Glycine and glutamic acid in hydrolyzed sample showed slight decreasing tendency in HADB. Mineral content was abundance in order of Ca $(1927{\sim}2379\;ppm),\;K\;(1159{\sim}1259ppm)$ and $Mg(760{\sim}777\;ppm)$. No difference was found in drying methods.

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Penetration of Some Humectants into Shark Muscle (상어육(肉)중의 수분유지제(水分維持劑) 침투(浸透))

  • Lee, Jong-Soo;Koo, Jae-Keun;Cha, Yong-Jun;Lee, Eung-Ho
    • Korean Journal of Food Science and Technology
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    • v.16 no.4
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    • pp.475-481
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    • 1984
  • Estimation of the penetration rate of humectants has been considered to be important in effective control of food processing when intermediate moisture food is manufactured by the moist-infusion method. In this study, when shark (Isurus glaucus) muscle was soaked in four common humectants (sucrose, sorbitol, glycerol, and propylene glycol), the equation of their penetration rate was drawn as a function of time using high performance liquid chromatography analysis. Penetration rates increased with soaking temperatures and decreased inversely with molecular weights of humectants. The penetrated amounts for 10% humectant solution reached about equilibrium after soaking for 10 hours and for 30% humectant after soaking for about 7 hours. In consideration with the penetration rate of the sample soaked in 10% humectant and complex solution of each 10% humectant, little difference was found between them. When the sample was soaked in 10% humectant and 30% humectant, it seemed to be able to apply the following regression equation to estimate the penetrated amounts: M = a log (c.t)+ b where M = penetrated amounts; c = concentration of humectant; t= soaking time; a, b = constant and c.t should be within $10^3\;-\;4{\times}10^4$.

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L-Methionine Production by Protoplast Fusion of Brevibacterium flavum ATCC 14067 and Corynebacterium glutamicum ATCC 13032 (Brevibacterium flavum ATCC 14067과 Corynebacterium glutamicum ATCC 13032의 원형질체 융합에 의한 L-Methionine의 생산)

  • Bin, Jae-Hoon;Chung, Soo-Ja;Shin, Dong-Bun;Ryu, Beung-Ho
    • Korean Journal of Food Science and Technology
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    • v.23 no.5
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    • pp.561-567
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    • 1991
  • This study was designed to investigate the productivity of L-methionine by the method of protoplast fusion between Brevibacterium flavum ATCC 14067 and Corynebacterium glutamicm ATCC 13032, and then L-methionine production was performed to continuous fermentation using the immobilized fusant cells. Mutants B. flavum K 104($thr\;met\;Km^{r}\;Et^{r}\;Sm^{r}\;Tm^{r}\;as\;genetic\;marker$) and C. glutamicum B 70($thr\;Hos\;Km^{r}\;Et^{r}\;Sm^{r}\;Tm^{r}as\;genetic\;marker$) were isolated by MNNG treatment. On the other hand, protoplast of mutants were formed to treat with lysis solution containing $500{\mu}g/ml$ of lysozyme. The ratios of protoplast formation and regeneration were 99% and $64{\sim}66%$ respectively. Fusion frequency between B. flavum K 104 and C. glutamicum B 70 showed the $3.5{\times}10^{5}$ in the 35% polyethylene glycol(PEG6000) containing 3% PVP solution. The productivity of L-methionine by fusant BFCG 37 immobilized with sodium alginate was 0.89 g/l the batch fermentation and was $18.75mg/^{1}hr\;^{1}$ on the continuous fermentation at $30^{\circ}C$ for 72 hr.

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Sensory and Quality Characteristics of Sanyakbyung Prepared with Different Amounts of Glutinous Rice Flour (찹쌀가루 첨가량에 따른 산약병의 기호성 및 품질특성에 관한 연구)

  • Yun, Sook-Ja;Jang, Myung-Sook
    • Korean journal of food and cookery science
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    • v.15 no.6
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    • pp.591-594
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    • 1999
  • An instrumental analysis of Sanyakbyung was carried out along with sensory evaluation to find out how its quality characteristics changed by the addition of glutinous rice flour(0%, 10%, 20%, 30%, 40% and 50%). The moisture content of Sanyakbyung decreased in proportion to the increment of glutinous rice flour. In terms of color, L-value increased depending on the increment of glutinous rice whereas a-value and b-value showed a decrease. This could be accounted for by two factors: one is that the protein contained in yam flour contributed to black coloring and the other is that the color of yam flour itself contributed ostensively to the tint of blackish coloring, and also to the tint of yellow and red coloring. ln the two bite compression test, the hardness, gumminess, chewiness, and cohesiveness of Sanyakbyung decreased depending on the increment of glutinous rice flour. The springiness did not show any significant variation among the samples. That is, the more yam flour was added, the more cohesive and gummy Sanyakbyung was produced. There were significant differences(p<0.05) in the sensory characteristics of the samples in which Sanyakbyung with 10% glutinous rice flour was most preferred in color, those with 20% in flavor, and those with 40% in taste. Adhesiveness and consistency in the palate responses also showed significant differences among samples in which the sample with 40% glutinous rice flour was most favored in consistency. When the content of yam increased, the gumminess also increased. Generally, Sanyakbyung with soft but less gummy texture was preferred to strong gummy one which was easily stuck to teeth. In the overall acceptance, Sanyakbyung with 40% glutinous rice four was most preferred.

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