고등소채류에 대한 세균학적 연구

BACTERIOLOGICAL STUDY ON CULTURED VEGETABLES

부산 근교에서 생산되는 고등 소채류에 대한 세균오염상태를 파악하고 세척, blanching등 처리방법에 따른 세균 제거율과 IMViC system에 의한 대장균군 동정시험 결과를 요약하면 다음과 같다. 1. 엽채류는 과채류에 비하여 분편소대장균의 오염도가 높았다. 2. 수도수로 세척했을 때 생균수 제거율에 있어서는 약 $70\%$, 엽채류에 있어서는 약 $20\%$ 있다. 3. 엽채류를 시판 서첵제로 세척했을 때 생균수 제거율을 약 $80\%$ 수도수로 세척했을때보다 $3\~4$배의 세척 효과가 없었다. 4. 2배의 끊는물에서 1분간 blanching했을 때 생균수 잔재율은 $0.1\%$ 이하있다. 5. 대장균군의 조성은 Eschrichia colr group $18\%$ Citrobacter freundii group $22\%$, Klebsiella aerogenes group $60\%$였으며 이중 Escherichia coli type I 이 $16\%$를 차지하였다. 6. 대장균군 확정시험 배지인 BGLB의 신선성은 약 $80\%$ 였다.

This experiment was carried out to evaluate the sanitary quality of cultured vegetables and to check the removing rate of bacteria by treating methods such as washing with tap water or commercial detergent, or blanching. Samples collected from farm land located at Busan suburbs and markets were Fragaria chiloensis var. ananasa, Lycopersicum esculentum, Capsium longum, Cucumis sativus, Lactuca scariola var. sativa leaf of Perilla frutescens var, japonica, Oananthe stolonifera and Allium odoium. Fecal coliform MPN was ra god from less than 30 to 430,000 per 100 grams of samples examined while plate count was $7.2\times10^3\;to\;2.2\times10^7$ per gram. Usually contamination rate of fecal coliform of leaf vegetal)les was much higher than that of fruit vegetables. Removing rate of bacterial density of vegetables by washing three times with tap water was about $70\%$ in fruit vegetables, about $20\%$ in leaf vegetables but it was about $80\%$ in leaf vegetables by washing with a commercial detergent. Sirvival rate of viable cell count of leaf vegetables was less than $0.1\%$ after blanching for one minutes in boiling tap water, Composition of coliform was $18\%$ Escherichia coli group, $22\%$ Citrobacter freundii group and $60\%$ Klebsiella aerogenes group, among Escherichia coli, type I being $16\%$ in total. The coliform detection from BGLB gas positive tubes being planted with various vegetables was about $80\%$ in the result of IMViC reaction.