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Cell clusters in intervertebral disc degeneration: an attempted repair mechanism aborted via apoptosis

  • Polly Lama;Jerina Tiwari;Pulkit Mutreja;Sukirti Chauhan;Ian J Harding;Trish Dolan;Michael A Adams;Christine Le Maitre
    • Anatomy and Cell Biology
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    • v.56 no.3
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    • pp.382-393
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    • 2023
  • Cell clusters are a histological hallmark feature of intervertebral disc degeneration. Clusters arise from cell proliferation, are associated with replicative senescence, and remain metabolically, but their precise role in various stages of disc degeneration remain obscure. The aim of this study was therefore to investigate small, medium, and large size cell-clusters. For this purpose, human disc samples were collected from 55 subjects, aged 37-72 years, 21 patients had disc herniation, 10 had degenerated non-herniated discs, and 9 had degenerative scoliosis with spinal curvature <45°. 15 non-degenerated control discs were from cadavers. Clusters and matrix changes were investigated with histology, immunohistochemistry, and Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Data obtained were analyzed with spearman rank correlation and ANOVA. Results revealed, small and medium-sized clusters were positive for cell proliferation markers Ki-67 and proliferating cell nuclear antigen (PCNA) in control and slightly degenerated human discs, while large cell clusters were typically more abundant in severely degenerated and herniated discs. Large clusters associated with matrix fissures, proteoglycan loss, matrix metalloproteinase-1 (MMP-1), and Caspase-3. Spatial association findings were reconfirmed with SDS-PAGE that showed presence to these target markers based on its molecular weight. Controls, slightly degenerated discs showed smaller clusters, less proteoglycan loss, MMP-1, and Caspase-3. In conclusion, cell clusters in the early stages of degeneration could be indicative of repair, however sustained loading increases large cell clusters especially around microscopic fissures that accelerates inflammatory catabolism and alters cellular metabolism, thus attempted repair process initiated by cell clusters fails and is aborted at least in part via apoptosis.

Purification and Characterization of Six Fibrinolytic Serine-Proteases from Earthworm Lumbricus rubellus

  • Cho, Il-Hwan;Choi, Eui-Sung;Lim, Hun-Gil;Lee, Hyung-Hoan
    • BMB Reports
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    • v.37 no.2
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    • pp.199-205
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    • 2004
  • The six lumbrokinase fractions (F1 to F6) with fibrinolytic activities were purified from earthworm Lumbricus rubellus lysates using the procedures of autolysis, ammonium sulfate fractionation, and column chromatography. The proteolytic activities on the casein substrate of the six iso-enzymes ranged from 11.3 to 167.5 unit/mg with the rank activity orders of F2 > F1 > F5 > F6 > F3 > F4. The fibrinolytic activities of the six fractions on the fibrin plates ranged from 20.8 to 207.2 unit/mg with rank orders of F6 > F2 > F5 > F3 > F1 > F4. The molecular weights of each iso-enzyme, as estimated by SDS-PAGE, were 24.6 (F1), 26.8 (F2), 28.2 (F3), 25.4 (F4), 33.1 (F5), and 33.0 kDa (F6), respectively. The plasminogen was activated into plasmin by the enzymes. The optimal temperature of the six iso-enzymes was $50^{\circ}C$, and the optimal pH ranged from pH 4-12. The four iso-enzymes (F1-F4) were completely inhibited by PMSF. The two enzymes (F5 and F6) were completely inhibited by aprotinin, TLCK, TPCK, SBTI, LBTI, and leupeptin. The N-terminal amino acid (aa) sequences of the first 20 to 22 residues of each fraction had high homology. All six isoenzymes had identical aa residues 2-3 and 13-15. The N-terminal 21-22 aa sequences of the F2, F3, and F4 isoenzymes were almost the same. The N-terminal aa sequences of F5 and F6 were identical.

Knowledge-based Semantic Meta-Search Engine (지식기반 의미 메타 검색엔진)

  • Lee, In-K.;Son, Seo-H.;Kwon, Soon-H.
    • Journal of the Korean Institute of Intelligent Systems
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    • v.14 no.6
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    • pp.737-744
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    • 2004
  • Retrieving relevant information well corresponding to the user`s request from web is a crucial task of search engines. However, most of conventional search engines based on pattern matching schemes to queries have a limitation that is not easy to provide results corresponding to the user`s request due to the uncertainty of queries. To overcome the limitation in this paper, we propose a framework for knowledge-based semantic meta-search engines with the following five processes: (i) Query formation, (ii) Query expansion, (iii) Searching, (iv) Ranking recreation, and (v) Knowledge base. From simulation results on english-based web documents, we can see that the Proposed knowledge-based semantic meta-search engine provides more correct and better searching results than those obtained by using the Google.

A Splog Detection System Using Support Vector Systems (지지벡터기계를 이용한 스팸 블로그(Splog) 판별 시스템)

  • Lee, Song-Wook
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.15 no.1
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    • pp.163-168
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    • 2011
  • Blogs are an easy way to publish information, engage in discussions, and form communities on the Internet. Recently, there are several varieties of spam blog whose purpose is to host ads or raise the PageRank of target sites. Our purpose is to develope the system which detects these spam blogs (splogs) automatically among blogs on Web environment. After removing HTML of blogs, they are tagged by part of speech(POS) tagger. Words and their POS tags information is used as a feature type. Among features, we select useful features with X2 statistics and train the SVM with the selected features. Our system acquired 90.5% of F1 measure with SPLOG data set.

A Study on the Design and Implementation of System for Predicting Attack Target Based on Attack Graph (공격 그래프 기반의 공격 대상 예측 시스템 설계 및 구현에 대한 연구)

  • Kauh, Janghyuk;Lee, Dongho
    • Journal of Korea Society of Digital Industry and Information Management
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    • v.16 no.1
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    • pp.79-92
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    • 2020
  • As the number of systems increases and the network size increases, automated attack prediction systems are urgently needed to respond to cyber attacks. In this study, we developed four types of information gathering sensors for collecting asset and vulnerability information, and developed technology to automatically generate attack graphs and predict attack targets. To improve performance, the attack graph generation method is divided into the reachability calculation process and the vulnerability assignment process. It always keeps up to date by starting calculations whenever asset and vulnerability information changes. In order to improve the accuracy of the attack target prediction, the degree of asset risk and the degree of asset reference are reflected. We refer to CVSS(Common Vulnerability Scoring System) for asset risk, and Google's PageRank algorithm for asset reference. The results of attack target prediction is displayed on the web screen and CyCOP(Cyber Common Operation Picture) to help both analysts and decision makers.

An Empirical Evaluation Analysis of the Performance of In-memory Bigdata Processing Platform (메모리 기반 빅데이터 처리 프레임워크의 성능개선 연구)

  • Lee, Jae hwan;Choi, Jun;Koo, Dong hun
    • Journal of Korea Society of Industrial Information Systems
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    • v.21 no.3
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    • pp.13-19
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    • 2016
  • Spark, an in-memory big-data processing framework is popular to use for real-time processing workload. Spark can store all intermediate data in the cluster memory so that Spark can minimize I/O access. However, when the resident memory of workload is larger that the physical memory amount of the cluster, the total performance can drop dramatically. In this paper, we analyse the factors of bottleneck on PageRank Application that needs many memory through experiment, and cluster the Spark with Tachyon File System for using memory to solve the factor of bottleneck and then we improve the performance about 18%.

A Study on the Recognition Analysis of Participants in Urban Regeneration Project Using Text Network Analysis Technique (NetMiner): Focused on the Urban Regeneration Leading Area in Suncheon-City

  • Gim, Eo-Jin;Koo, Ja-Hoon
    • International Journal of Advanced Culture Technology
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    • v.7 no.4
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    • pp.246-254
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    • 2019
  • The purpose of this study is to analyze the issues related to urban regeneration project at the present time through surveys and interviews of participants in the urban regeneration leading project of Suncheon city. Most of the comments were related to business fragmentation and things that should be improved in the future. The text network technique is applied to the subject analysis using unstructured text data. As a result of the frequency of appearance and analysis of page rank centrality between words, words of 'parking', 'need', 'lack', 'region' and 'resident' appeared at the top, and the result of analyzing the mediation centrality of key words showed 'culture', 'Need', 'region', 'inflow' and 'lack' appeared at the top. In the network analysis, the most central words appeared, and many words appeared in the important position in the sentence. Text network analysis has provided timely results in terms of sustainability after completion of the Suncheon City Regeneration Leading Project..

A Systematic Literature Review on Service Quality: Bibliomertics and Network Analysis (서비스 품질의 체계적 문헌 조사 연구: 계량서지학과 네트워크 분석을 중심으로)

  • Jeong, EuiBeom;Park, Jinsoo
    • Journal of Korean Society for Quality Management
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    • v.47 no.2
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    • pp.327-344
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    • 2019
  • Purpose: This study aims to conduct a systematic literature review to suitably identify wide and specific issues and topics on service quality in supply chain. Methods: This study is to investigate service quality in supply chain research using a systematic literature review methodology. In order to extract influential journals and papers, we used the SJR impact factor provided by the SCOPUS database. The collected 169 papers were analyzed using bibliometric analysis, citation analysis as well as keywords network. Results: We conducted a bibliometric analysis to identify top authors contributing to service quality in supply chain and their issues, and further examined important keywords and new emerging keywords. In addition, we extracted five influential papers by PageRank to clarify critical issues and divided into five clusters to identify topics of service quality in supply chain by using network-based approach. In order to examine comprehensive issues and topics of service quality in supply chain, we constructed a keyword network to observe difference in the classification of important keywords across network centrality measures. Conclusion: Our study reviewed literature on service quality in supply chain and explored the future directions and trends of service quality in supply chain.

Improving the Yield of Semiconductor Manufacturing Processes using Clustering Analysis and Response Surface Method (군집분석 및 반응표면분석법을 활용한 반도체 공정 수율향상에 관한 연구)

  • Koh, Kwan Ju;Kim, Na Yeon;Kim, Yong Soo
    • Journal of Korean Society for Quality Management
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    • v.47 no.2
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    • pp.381-395
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    • 2019
  • Purpose: This study aims to conduct a systematic literature review to suitably identify wide and specific issues and topics on service quality in supply chain. Methods: This study is to investigate service quality in supply chain research using a systematic literature review methodology. In order to extract influential journals and papers, we used the SJR impact factor provided by the SCOPUS database. The collected 169 papers were analyzed using bibliometric analysis, citation analysis as well as keywords network. Results: We conducted a bibliometric analysis to identify top authors contributing to service quality in supply chain and their issues, and further examined important keywords and new emerging keywords. In addition, we extracted five influential papers by PageRank to clarify critical issues and divided into five clusters to identify topics of service quality in supply chain by using network-based approach. In order to examine comprehensive issues and topics of service quality in supply chain, we constructed a keyword network to observe difference in the classification of important keywords across network centrality measures. Conclusion: Our study reviewed literature on service quality in supply chain and explored the future directions and trends of service quality in supply chain.

Learning Material Bookmarking Service based on Collective Intelligence (집단지성 기반 학습자료 북마킹 서비스 시스템)

  • Jang, Jincheul;Jung, Sukhwan;Lee, Seulki;Jung, Chihoon;Yoon, Wan Chul;Yi, Mun Yong
    • Journal of Intelligence and Information Systems
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    • v.20 no.2
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    • pp.179-192
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    • 2014
  • Keeping in line with the recent changes in the information technology environment, the online learning environment that supports multiple users' participation such as MOOC (Massive Open Online Courses) has become important. One of the largest professional associations in Information Technology, IEEE Computer Society, announced that "Supporting New Learning Styles" is a crucial trend in 2014. Popular MOOC services, CourseRa and edX, have continued to build active learning environment with a large number of lectures accessible anywhere using smart devices, and have been used by an increasing number of users. In addition, collaborative web services (e.g., blogs and Wikipedia) also support the creation of various user-uploaded learning materials, resulting in a vast amount of new lectures and learning materials being created every day in the online space. However, it is difficult for an online educational system to keep a learner' motivation as learning occurs remotely, with limited capability to share knowledge among the learners. Thus, it is essential to understand which materials are needed for each learner and how to motivate learners to actively participate in online learning system. To overcome these issues, leveraging the constructivism theory and collective intelligence, we have developed a social bookmarking system called WeStudy, which supports learning material sharing among the users and provides personalized learning material recommendations. Constructivism theory argues that knowledge is being constructed while learners interact with the world. Collective intelligence can be separated into two types: (1) collaborative collective intelligence, which can be built on the basis of direct collaboration among the participants (e.g., Wikipedia), and (2) integrative collective intelligence, which produces new forms of knowledge by combining independent and distributed information through highly advanced technologies and algorithms (e.g., Google PageRank, Recommender systems). Recommender system, one of the examples of integrative collective intelligence, is to utilize online activities of the users and recommend what users may be interested in. Our system included both collaborative collective intelligence functions and integrative collective intelligence functions. We analyzed well-known Web services based on collective intelligence such as Wikipedia, Slideshare, and Videolectures to identify main design factors that support collective intelligence. Based on this analysis, in addition to sharing online resources through social bookmarking, we selected three essential functions for our system: 1) multimodal visualization of learning materials through two forms (e.g., list and graph), 2) personalized recommendation of learning materials, and 3) explicit designation of learners of their interest. After developing web-based WeStudy system, we conducted usability testing through the heuristic evaluation method that included seven heuristic indices: features and functionality, cognitive page, navigation, search and filtering, control and feedback, forms, context and text. We recruited 10 experts who majored in Human Computer Interaction and worked in the same field, and requested both quantitative and qualitative evaluation of the system. The evaluation results show that, relative to the other functions evaluated, the list/graph page produced higher scores on all indices except for contexts & text. In case of contexts & text, learning material page produced the best score, compared with the other functions. In general, the explicit designation of learners of their interests, one of the distinctive functions, received lower scores on all usability indices because of its unfamiliar functionality to the users. In summary, the evaluation results show that our system has achieved high usability with good performance with some minor issues, which need to be fully addressed before the public release of the system to large-scale users. The study findings provide practical guidelines for the design and development of various systems that utilize collective intelligence.