• 동의생리병리학회지
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• 제16권3호
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• pp.563-566
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• 2002

To evaluate the effect of Jingansikpung-tang(JST) water extract on cultured mouse spinal sensory neuron which was inhibited by glucose oxidase(GO)-induced cytotoxicity, MTT and LDH activity assay were carried out after the cultured mouse spinal sensory neuron were pre-incubated with various concentrations of JST extract for 3 hours prior to exposure of GO. The results obtained were as follows: GO, a oxygen radical, decreased the survival rate of the cultured mouse spinal sensory neuron cells on MTT assay. JST water extract have efficacy of decreasing LDH activity increasing by GO in cultured mouse spinal sensory neuron. From above the results, it is concluded that JST water extract has marked efficacy as a treatment for the damages caused in the GO-mediated oxidative process.

• 동의생리병리학회지
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• 제17권2호
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• pp.374-379
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• 2003

To evaluate the mechanism of oxidative damage by Xanthine oxidase(XO) and hypoxanthine(HX)-induced oxygen radicals, XTT assay was carried out. Neurofilament EIA and PKC activity were measured to evaluate the protective effect of Jingansikpung-tang(JST) and Gamijingansikpung-tang(GJST) water extract on cultured spinal sensory neurons damaged by XO/HX, after the cultured mouse spinal sensory neurons were preincubated with various concentrations of JST and GJST water extract for 3 hours prior to exposure of XO/HX. The results were XO/HX decreased significantly, in proportion to concentration and exposed time, the survival rate of the cultured mouse sensory neurons on XTT assay. And in proportion to concentration and exposed time on cultured spinal sensory neurons, XO/HX showed the quantitative decrease of neurofilament by EIA, increase of PKC activity, but JST and GJST showed the neuroprotective effects against decrease of neurofilament and increase of PKC activity by XO/HX. From the above results, it is concluded that XO/HX have a neurotoxic effect on cultured spinal sensory neurons and the herbs water extract, such as JST and GJST prevent the toxicity of XO/HX effectively.

• 대한한방내과학회지
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• 제23권1호
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• pp.47-56
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• 2002

Objectives: We identified the effects of Jingansikpung-tang, (JGSPT), derived from therapy on closed head trama(CHT)-induced brain edema and neurologial disturbance symptom in rats. Methods : We used a drop device and induced CHT. We divided the animals into 3 groups. They were JGSPT 1 group (Administered JGSPT 96.5mg/kr through anal far one hour after using CHT) and JGSPT 2 group(Administered JGSPT 386mg/kg through anal for one hour after using CHT) Then, we tested neurological severity score(NSS), water content of brain and haematological changes. Result : aNSS increased significantly in JGSPT 1 and JGSPT 2 as compared to the controlled group, and the change of water content which was pulled out of the right hemisphere from the left hemisphere decreased in JGSPT 1 and JGSPT 2 as compared to the controlled group. The lactate level in serum decreased in JGSPT 1 and JGSPT 2 as compared to the controlled group, which showed no efficacy. Glucose level in serum increased in JGSPT 1 and JGSPT 2 as compared to the controlled group, only JGSPT 2 showed no efficacy. Conclusions : JGSPT, caused by Anal Therapy and effect on CHT-induced brain edema and neurological symptom.