Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Effects of Jingansikpung-tang and Gamijingansikpung-tang Water Extract on the Cultured Spinal Sensory Neurons

진간식풍탕 및 가미진간식풍탕 추출물이 배양 척수감각신경세포에 미치는 영향

  • Seo Young Suk (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Yun Sang Hak (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Yeom Seung Ryong (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Lee Su kyung (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Shin Byung Cheul (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Kwon Young Dal (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Song Yung Sun (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Wonkwang University)
  • 서영석 (원광대학교 한의과대학 한방재활의학과교실) ;
  • 윤상학 (원광대학교 한의과대학 한방재활의학과교실) ;
  • 염승룡 (원광대학교 한의과대학 한방재활의학과교실) ;
  • 이수경 (원광대학교 한의과대학 한방재활의학과교실) ;
  • 신병철 (원광대학교 한의과대학 한방재활의학과교실) ;
  • 권영달 (원광대학교 한의과대학 한방재활의학과교실) ;
  • 송용선 (원광대학교 한의과대학 한방재활의학과교실)
  • Published : 2003.04.01
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Abstract

To evaluate the mechanism of oxidative damage by Xanthine oxidase(XO) and hypoxanthine(HX)-induced oxygen radicals, XTT assay was carried out. Neurofilament EIA and PKC activity were measured to evaluate the protective effect of Jingansikpung-tang(JST) and Gamijingansikpung-tang(GJST) water extract on cultured spinal sensory neurons damaged by XO/HX, after the cultured mouse spinal sensory neurons were preincubated with various concentrations of JST and GJST water extract for 3 hours prior to exposure of XO/HX. The results were XO/HX decreased significantly, in proportion to concentration and exposed time, the survival rate of the cultured mouse sensory neurons on XTT assay. And in proportion to concentration and exposed time on cultured spinal sensory neurons, XO/HX showed the quantitative decrease of neurofilament by EIA, increase of PKC activity, but JST and GJST showed the neuroprotective effects against decrease of neurofilament and increase of PKC activity by XO/HX. From the above results, it is concluded that XO/HX have a neurotoxic effect on cultured spinal sensory neurons and the herbs water extract, such as JST and GJST prevent the toxicity of XO/HX effectively.

Keywords

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