• Journal of Digital Convergence
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• v.11 no.1
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• pp.299-308
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• 2013

We investigated the effect of mulberry leaf extract (MLE) on blood biochemical parameters of white rats induced TCDD toxicity. Data were analyzed by One-way ANOVA and Post-Hoc Test. The numbers of leukocyte and platelet decreased by TCDD were increased dramatically after treated MLE (p<0.01). The concentration of calcium and magnesium decreased by TT were increased by MLE and shown a statistical significance (p<0.01) but the concentration of phosphorus increased by TT was decreased by MLE. In the test of parameters related to renal function, only the concentration of uric acid in the MLE group was decreased than it in TT group and shown a statistical significance (p<0.01). Also the concentration of blood glucose and the activity of amylase and lipase increased by TT were decreased after treated MLE and shown a statistical significance (p<0.01), and MLE had an control effect for elevating blood glucose. In the test of parameters related to liver function, the acticity of AST, ALT and ALP increased by TT was decreased after treated MLE and shown a statistical significance (p<0.01), and MLE had an improving effect for liver function. In the case of total cholesterol (T,cho), triglyceride( TG), LDL-cholesterol (LDL-C) and HDL-cholesterol (HDL-C) related to lipid metabolism, the amount of T,cho, TG, LDL-C increased by TT were decreased after treated MLE and shown a statistical significance (p<0.01), and MLE had an ability of anti-hyperlipidemia. From these results, we concluded that MLE could mollified TCDD toxicity in white rats exposed to TCDD.

• Journal of Microbiology and Biotechnology
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• v.22 no.10
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• pp.1401-1405
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• 2012

A thermotolerant Saccharomyces cerevisiae mutant strain, TT6, was constructed after multi-parental hybridization of five mutant strains obtained by UV or NTG treatment of the original strain, S. cerevisiae KV1. When incubated at $40^{\circ}C$ in YPD broth, TT6 began to grow exponentially in 10 h, but KV1 did not show any noticeable growth even after 22 h. The thermotolerant growth of TT6 was confirmed by serial dilution assay at $42^{\circ}C$; TT6 grew at a cell concentration ($10^{-5}$) 10,000 times lower than that of KV1 ($10^{-1}$). Whereas ethanol production from YP containing 23% (w/v) glucose by KV1 decreased with increasing temperature from $30^{\circ}C$ to $36^{\circ}C$, ethanol production by TT6 did not decrease at temperatures up to $37^{\circ}C$. When TT6 was tested for ethanol production at $36^{\circ}C$ by simultaneous saccharification and fermentation (SSF) from 23% corn, 24 h of fermentation time or 50% of the glucoamylase dose was saved when compared with KV1 at $30^{\circ}C$. The ethanol yield from corn by SSF with TT6 at $36^{\circ}C$ was 91.7% of the theoretical yield, whereas that of KV1 at $30^{\circ}C$ was 90.6%.

• Journal of Microbiology and Biotechnology
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• v.29 no.12
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• pp.1938-1946
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• 2019

Isomaltooligosaccharides (IMOs) have good prebiotic effects, and long IMOs (LIMOs) with a degree of polymerization (DP) of 7 or above show improved effects. However, they are not yet commercially available, and require costly enzymes and processes for production. The N-terminal region of the thermostable Thermoanaerobacter thermocopriae cycloisomaltooligosaccharide glucanotransferase (TtCITase) shows cyclic isomaltooligosaccharide (CI)-producing activity owing to a catalytic domain of glycoside hydrolase (GH) family 66 and carbohydrate-binding module (CBM) 35. In the present study, we elucidated the activity of the C-terminal region of TtCITase (TtCITase-C; Met740-Phe1,559), including a CBM35-like region and the GH family 15 domain. The domain was successfully cloned, expressed, and purified as a single protein with a molecular mass of 115 kDa. TtCITase-C exhibited optimal activity at 40℃ and pH 5.5, and retained 100% activity at pH 5.5 after 18-h incubation. TtCITase-C synthesized α-1,6 glucosyl products with over seven degrees of polymerization (DP) by an α-1,6 glucosyl transfer reaction from maltopentaose, isomaltopentaose, or commercialized maltodextrins as substrates. These results indicate that TtCITase-C could be used for the production of α-1,6 glucosyl oligosaccharides with over DP7 (LIMOs) in a more cost-effective manner, without requiring cyclodextran.

• Archives of Pharmacal Research
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• v.28 no.9
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• pp.1037-1041
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• 2005

Geranium (Pelargonium inquinans Ait) leaves were extracted with $80\%$ MeOH, and partitioned into n-hexane, ethyl acetate, BuOH and $H_2O$ to isolate the anticoagulant principles. The EtOAc fraction was found to be the most active, and was further purified using silica and octadecylisilane column chromatography employing a bioassay-guided fractionation method. The active compound was isolated and identified as $1,2,3,4,6-pentagalloyl-\beta-D-glucopyranose$(PGG) (compound I). The isolated anticoagulant significantly prolonged the activated partial thrombin time (APTT) and thrombin time (TT) using normal human plasma. One microgram of $1,2,3,4,6-pentagalloyl-\beta-D-glucopyranose$ showed 0.063 heparin units in the APTT and 2.73 heparin units in the TT for anti-thrombosis. This is the first report of the isolation of PGG from geranium plants.

• Transactions of the Korean Society of Pressure Vessels and Piping
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• v.20 no.1
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• pp.16-24
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• 2024

Denting of steam generator (SG) tube is defined as the reduction in tube diameter due to the stresses exerted by the corrosion products formed on the outer diameter surface. This phenomenon is mostly observed in the crevices between SG tube and the top-of tubesheet or tube support plate. Despite the replacement of SG tube with Alloy 690, which has better corrosion resistance than Alloy 600, the denting of SG tube still remains a potential problem that could decrease the SG integrity. Deformation of SG tube by denting phenomenon can affect the surface properties and microstructure of SG tube. In this study, the effects of plastic deformation on surface properties and microstructure of Alloy 690 thermally treated (TT) tube was investigated by using the various analysis techniques. The plastic deformation of Alloy 690 increased the surface roughness and area. Many surface defects such as ripped surface and micro-cracks were observed on the deformed Alloy 690TT specimen. Based on the electron backscatter diffraction analysis, the dislocation density of deformed SG tube increased compared to non-deformed SG tube. In addition, the effects of changes in surface properties and microstructure of SG tube on general corrosion behavior were discussed.

• Journal of Life Science
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• v.23 no.12
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• pp.1460-1470
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• 2013

To examine whether miscellaneous cereal grains have an antithrombotic effect, we investigated the anticoagulant activity of 80% ethanol extracts from eleven selected miscellaneous cereal grains. The 80% ethanol extract of hwanggeumchal sorghum (Sorghum bicolor) showed the highest anticoagulant activity, followed by that of green foxtail millet grains, in terms of thrombin time (TT). When the ethanol extract of hwanggeumchal sorghum was sequentially fractionated with n-hexane, methylene chloride, ethyl acetate, and n-butanol, the majority of the TT-inhibitory activity was detected in the hexane and methylene chloride fractions. Whereas aspirin (final conc. 480 ${\mu}g/ml$) prolonged TT by 2-fold, the ethanol extract, hexane fraction, and methylene chloride fraction in the same dose prolonged TT by 2.2-fold, 2.9-fold, and 2.5-fold, respectively. The ethanol extract of hwanggeumchal sorghum could delay activated partial thromboplastin time (APTT) as well as prothrombin time (PT). Although the APTT-inhibitory activity of the ethanol extract was mainly partitioned into the hexane and methylene chloride fractions, the PT-inhibitory activity of the ethanol extract was solely partitioned into the hexane fraction. The APTT- and PT-inhibitory activities of these organic solvent fractions were more potent than those of the control warfarin (final conc. 3.13 mg/ml). The TT-inhibitory activity of the ethanol extract was heat-stable and acid-stable. The ethanol extract, hexane fraction, and methylene chloride fraction of hwanggeumchal sorghum appeared to possess a direct fibrinolytic activity toward fibrin clotting. These results show that hwanggeumchal sorghum can exert anticoagulant and fibrinolytic effects and, thus, have the potential to be applicable as antithrombotic dietary sources.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.2
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• pp.701-705
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• 2013

Polymorphisms in XPG are considered to contribute to the clinical outcome of patients receiving platinum drug chemotherapy. We aimed to investigate the role of five potential SNPs of XPG gene on the response to platinum-based chemotherapy in advanced Chinese NSCLC patients. A total of 451 patients with newly diagnosed and histopathologically confirmed primary NSCLC were consecutively collected. XPG rs2296147, rs4150261, rs17655, rs1047768 and rs2094258 were genotyped by the Taqman real-time polymerase chain reaction (PCR). In our study, we found patients carrying rs1057768 TT genotype had a significantly lower treatment response when compared with the CC genotype (OR=0.38, 95% CI=0.18-0.78). Patients carrying rs1047768 TT genotype showed a significantly short median PFS (11.2 months) and OS (13.6 months) than CC genotype, and the hazard ratios (HR) for PFS and OS were 2.06 (1.01-4.50) and 2.29 (1.21-2.49), respectively. Moreover, we found a significant decreased risk of death from NSCLC among patients carrying the rs2296147 TT genotype when compared with the CC genotype, the HR (95% CI) for OS being 0.50 (0.27-0.95). In conclusion, our study found that polymorphisms in rs1047768 C/T and rs2296147 C/T are associated with response to platinum-based chemotherapy in advanced NSCLC, and XPG polymorphisms could be predictive of prognosis.

• Journal of the Korean Chemical Society
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• v.52 no.6
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• pp.703-711
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• 2008

• Proceedings of the KAIS Fall Conference
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• 2011.05b
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• pp.892-896
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• 2011

영어 교육용 소프트웨어는 다양한 기법을 적용하여 개발, 활용되고 있으며 영어 능력을 중요시하는 현재의 시대상을 반영하듯 학교, 학원, 직장 외에 개인 PC를 이용해 시간과 장소의 제약 없이 쉽고, 재미있게 영어 공부를 돕는데 목적이 두고 지속적으로 개발되고 있다. 따라서 본 논문에서는 영어 교육의 중요성을 높게 판단하여 영어 단어를 중점적으로 학습하는데 목표를 둔 시스템을 구현하였다. 즉, TTS를 활용하여 음성적 교육 방식을 적용하였으며 영어 단어 학습 과정과 문제 풀이 과정으로 분류하여 영어 공부에 대한 흥미를 유발시키는 시스템을 설계하였으며 개인 계정을 통한 학습 과정 진도를 저장 및 관리하는 시스템 환경을 구현하였다.

• BMB Reports
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• v.40 no.5
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• pp.832-838
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• 2007

A novel inhibitory protein against blood coagulation factor Va (FVa) was purified from muscle protein of granulated ark (Tegillarca granosa, order Arcoida, marine bivalvia) by consecutive FPLC method using anion exchange and gel permeation chromatography. In the results of ESI-QTOF tandem mass analysis and database research, it was revealed that the purified T. granosa anticoagulant protein (TGAP) has 7.7 kDa of molecular mass and its partial sequence, HTHLQRAPHPNALGYHGK, has a high identity (64%) with serine/threonine kinase derived from Rhodopirellula baltica (order Planctomycetales, marine bacteria). TGAP could potently prolong thrombin time (TT), corresponding to inhibition of thrombin (FIIa) formation. Specific factor inhibitory assay showed that TGAP inhibits FVa among the major components of prothrombinase complex. In vitro assay for direct-binding affinity using surface plasmon resonance (SPR) spectrometer indicated that TGAP could be directly bound with FVa. In addition, the binding affinity of FVa to FII was decreased by addition of TGAP in dose-dependant manner ($IC_{50}$ value = 77.9 nM). These results illustrated that TGAP might interact with a heavy chain of FVa ($FVa_H$) bound to FII in prothrombin complex. The present study elucidated that non-cytotoxic T. granosa anticoagulant protein (TGAP) bound to FVa can prolong blood coagulation time by inhibiting conversion of FII to FIIa in blood coagulation cascade. In addition, TGAP did not significantly (P < 0.05) show fibrinolytic activity and cytotoxicity on venous endothelial cell line (ECV 304).