Archives of Pharmacal Research

The Pharmaceutical Society of Korea (대한약학회)
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Anticoagulant 1,2,3,4,6-Pentagalloyl-$\beta$-D-Glucopyranose Isolated from Geranium (Pelargonium inquinans Ait)

  • Ji Myeong-Sim (Department of Agricultural Chemistry and Institute of Agricultural Science and Technology, Chonnam National University) ;
  • Piao Xiang-Lan (College of Pharmacy, Seoul National University) ;
  • Jin Yu-Lan (Department of Plant Protection, Laiyang Agricultural College) ;
  • Park Ro Dong (Department of Agricultural Chemistry and Institute of Agricultural Science and Technology, Chonnam National University)
  • Published : 2005.09.01
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Abstract

Geranium (Pelargonium inquinans Ait) leaves were extracted with $80\%$ MeOH, and partitioned into n-hexane, ethyl acetate, BuOH and $H_2O$ to isolate the anticoagulant principles. The EtOAc fraction was found to be the most active, and was further purified using silica and octadecylisilane column chromatography employing a bioassay-guided fractionation method. The active compound was isolated and identified as $1,2,3,4,6-pentagalloyl-\beta-D-glucopyranose$(PGG) (compound I). The isolated anticoagulant significantly prolonged the activated partial thrombin time (APTT) and thrombin time (TT) using normal human plasma. One microgram of $1,2,3,4,6-pentagalloyl-\beta-D-glucopyranose$ showed 0.063 heparin units in the APTT and 2.73 heparin units in the TT for anti-thrombosis. This is the first report of the isolation of PGG from geranium plants.

Keywords

References

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