• Journal of Embryo Transfer
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• v.32 no.4
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• pp.275-285
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• 2017

Lysophosphatidic acid (LPA) is an important signaling molecule. Here, the effect and mechanism of LPA on the preimplantation development of porcine embryos during in vitro culture (IVC) was examined. Porcine embryos were cultured in porcine zygote medium (PZM-3) supplemented with $30{\mu}M$ LPA during different days. There was a significantly higher cleavage rate in Day 1-7 and significantly higher total cell number of blastocysts in Day 1-3 and Day 4-7. It was also found that messenger RNA (mRNA) expression level of PCNA, BCL2 and BAX in blastocysts obtained from D1-7 group were significantly higher and BCL2/BAX mRNA ratio in D1-3 group was significantly lower than control group but Day 4-7 and Day 1-7 groups were comparable with control group. Treatment with $20{\mu}M$ PLC inhibitor significantly decreased the embryo cleavage rate and blastocyst formation rate. Moreover, LPA as an activator of PLCs, enhanced the $30{\mu}M$ LPA + $20{\mu}M$ U73122 group embryo cleavage rate which similar with control group. In conclusion, the results suggest that treatment with LPA during IVC improves the porcine early embryo cleavage by activation of PLC signaling pathway and regulate the mRNA expression that contribute to total cell number of blastocysts during blastocyst formation.

• Korean Journal of Crystallography
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• v.8 no.1
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• pp.1-5
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• 1997

The crystal structure of the title compound consists of discrete 4-N,N-dimethylamino-4'-N'-methyl-stilbazolium, $C_{16}H_{19}N_2$, and tosylate, $C_7H_7SO_3$, dimer. The 4-N,N-dimethylamino-4'-N'-methyl-stilbazolium molecule has a trans conformation at the central C=C double bond: the dihedral angle between the phenyl and the pyridyl rings is $5.7(2)^{\circ}$ and the whole molecule is planar within $0.138(8){\AA}$. Tosylate molecules display hydrogen-bonded dimers with the O-H...O distances of 2.855(9) and $2.899(8){\AA}$, respectively. The shortest intermolecular contact is the distance $3.10(1){\AA}$ between O(3) and C(16).

• Transactions of the Korean Society of Mechanical Engineers B
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• v.39 no.1
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• pp.61-69
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• 2015

The objective of this study is to experimentally investigate the mixed flow behaviors and oxygen transfer characteristics of a vertical orifice ejector. The experimental apparatus consisted of an electric motor-pump, an orifice ejector, a circulation water tank, an air compressor, a high speed camera unit and control or measurement accessories. The mass ratio was calculated using the measured primary flow rate and suction air flow rate with experimental parameters. The visualization images of vertically injected mixed jet issuing from the orifice ejector were qualitatively analyzed. The volumetric oxygen transfer coefficient was calculated using the measured dissolved oxygen concentration. At a constant primary flow rate, the mass ratio and oxygen transfer coefficient increase with the air pressure of compressor. At a constant air pressure of the compressor, the mass ratio decreases and the oxygen transfer coefficient increases as the primary flow rate increases. The residence time and dispersion of fine air bubbles and the penetration of mixed flow were found to be important parameters for the oxygen transfer rate owing to the contact area and time of two phases.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.40 no.8
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• pp.503-510
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• 2016

Incinerators generally emit pollutants such as NOx and CO during the combustion process. In this paper, pollutant emissions and temperature distributions were studied in a simulated incinerator with a reversed (relative to the flue gas flow) secondary air injection system. The experiments were performed by using a lab-scale furnace in order to evaluate the effects of the injection location, direction and flow rate of secondary air jets. The emission of NOx was lower in the case of reversed secondary air injection than in the case of cross injection, due to the recirculation and mixing of the exhaust gas. In the reversed air injection cases, thermal NOx emissions decreased as secondary air ratio increased from 30 to 60 and slightly increased at secondary air ratios higher than 60. In most cases, CO emissions were not detected except for a few reversed secondary air injection cases, in which cases CO concentrations below 2ppm were observed.

• Journal of the Korean Institute of Gas
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• v.16 no.4
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• pp.59-64
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• 2012

The dispersion of gas discharged from the vent pipes of pressure relief valves attached LPG (Liquefied Petroleum Gas) storage tank was studied. In general, vent pipes should be positioned so that they discharge vertically upwards in a safe place, and installed so that, in the event of ignition of discharged gas, flame impingement on any vessel, equipment or piping is avoided[1][2]. In Korea, on the other hand, there are various type of the end of vent pipes because there is no rule for discharge directions from the vent pipes. In this paper, we took 4 types of vent directions from the pipes in to account, such as vertically upward, vertically downward, vertically 4-way and horizontally 2-way direction. A software package, FLACS, was adopted to simulate gas dispersion from the vent pipes. We found that vertically downward, vertically 4-way and horizontally 2-way discharge from vent pipes were undesirable to avoid ignition on near ground. Therefore, it was obvious that vertically upward opening of a vent pipe is the best option to discharge in a safe place.

• Fire Science and Engineering
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• v.30 no.4
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• pp.39-45
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• 2016

The optimal design of an air curtain nozzle installed at exits, such as fire doors, was determined in order to block the flow of smoke into safe zones. Smoke is the greatest cause of loss of life during the fire. To block the flow of smoke, the airflow must be expelled uniformly without eccentricity from the slits in the air curtain nozzle installed on the upper part of the opening. In order to accomplish this, factors such as air inflow volume, shape of the internal slits, and thickness of the external slits were considered as variables in this study, and a numerical analysis was performed under various conditions. This led to the selection of a final shape which led to the finalization of a design shape. The final shape was manufactured as a prototype and the results were compared and verified with the results of the numerical analysis. The relative error of the numerical analysis results was less than 1%, and the average speed of all the slits was tested, exhibiting a highly consistent tendency.

• Geomechanics and Engineering
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• v.30 no.6
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• pp.565-577
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• 2022

In this study, a series of three-dimensional numerical parametric study was conducted to investigate deformation mechanisms of an existing box culvert due to an adjacent multi-propped basement excavation in clays. Field measurements from an excavation case history are first used to calibrate a baseline Hardening Soil Small Strain (HS-small) model, which is subsequently adopted for parametric study. Results indicate that the basement-box culvert interaction along the basement centerline can be considered as a plane strain condition when the length of excavation (L) reaches 14 H_e (i.e., final excavation depth). If a plane strain condition (i.e., L/H_e=12.0) is assumed for analyzing the basement-box culvert interaction of a short excavation (i.e., L/H_e=2.0), the maximum settlement and horizontal movement of the box culvert are overestimated significantly by up to 15.7 and 5.1 times, respectively. It is also found that the deformation of box culvert can be greatly affected by the basement excavation if the distance between the box culvert and retaining wall is less than 1.5 H_e. The induced deformation in the box culvert can be dramatically reduced by improving the ground inside the excavation or implementing other precautionary measures. For example, by adding jet grouting columns within the basement and installing an isolation wall behind the retaining structures, the maximum settlements of box culvert are shown to reduce by 37.2% and 13.4%, respectively.

• Atmosphere
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• v.33 no.5
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• pp.441-456
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• 2023

The mobile observation method, in which a meteorological drone observes while ascending, can observe the vertical profile of wind at 1 m-interval. In addition, since continuous flights are possible at time intervals of less than 30 minutes, high-resolution observation data can be obtained both spatially and temporally. In this study, we verify the accuracy of mobile observation data from meteorological drone (drone) and fill the spatio-temporal observation gaps in the lower atmosphere. To verify the accuracy of mobile observation data observed by drone, it was compared with rawinsonde observation data. The correlation coefficients between two equipment for a wind speed and direction were 0.89 and 0.91, and the root mean square errors were 0.7 m s^-1 and 20.93°. Therefore, it was judged that the drone was suitable for observing vertical profile of the wind using mobile observation method. In addition, we attempted to resolve the observation gaps in the lower atmosphere. First, the vertical observation gaps of the wind profiler between the ground and the 150 m altitude could be resolved by wind observation data using the drone. Secondly, the temporal observation gaps between 3-hour interval in the rawinsonde was resolved through a drone observation case conducted in Taean-gun, Chungcheongnam-do on October 13, 2022. In this case, the drone mobile observation data every 30-minute intervals could observe the low-level jet more detail than the rawinsonde observation data. These results show that the mobile observation data of the drone can be used to fill the spatio-temporal observation gaps in the lower atmosphere.

• Plant Journal
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• v.16 no.1
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• pp.34-37
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• 2020

The need to improve the performance of the dust collector by increasing the fine dust pollution in Korea is eminent. In this study, electrostatic spray wet electrostatic precipitator is used to remove Particulate Matter (PM). In order to enter the Cone-jet mode, which is the most stable spray mode among the various modes of electrostatic spraying, an appropriate voltage-flow condition must be satisfied, and a voltage-flow interval enabling this can be expressed as Stability Island. The voltage at which the conduit mode starts in the Stability Island section is referred to as the electrostatic spray minimum voltage (Onset Voltage). However, the conventional electrostatic spray is uses a very low flowrate, at most few millilitres per minute. The use of much higher flowrates has introduced different patterns of electrospray modes. In this study, the different spray modes will be studied in detail.

• Journal of Embryo Transfer
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• v.29 no.2
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• pp.183-188
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• 2014

A study was carried out on 16 indigenous ewes in Bangladesh in order to assess the reproductive physiology, the pattern of vaginal cell exfoliation and progesterone profiles during the estrous cycle period. The mean estrous cycle length and duration of estrus were $15.8{\pm}0.12$ days and $31.1{\pm}0.57$ h respectively. The exfoliated epithelial cells were categorized into parabasal, intermediate, superficial and keratinized and their relative occurrences. The percentages of parabasal, intermediate and superficial cell type during proestrus were similar. The percentage of superficial cell type during estrus was 61.7%, which was significantly (p<0.01) differ from other types of cells and stages of estrus cycle. Metoestrus was predominant with neutrophils in addition with other cell types. Dioestrus was dominated by neutrophils. On days 0 to 5 of the cycle the progesterone concentration was 0.09 to $1.6{\pm}0.07ng/ml$. The length of diestrus was 5~10 days with a range of mean progesterone level of $1.6{\pm}0.07$ to $2.8{\pm}0.11ng/ml$. Progesterone levels increased significantly (p<0.01) after Day 5 and maximum level was $2.8{\pm}0.11ng/ml$ observed on Day 10 of the estrous cycle. Thereafter it dropped rapidly to basal level of $0.11{\pm}0.04ng/ml$ on Day 0 (p<0.01). These results indicate that the pattern of exfoliation of vaginal cells along with progesterone concentration could be used to determine the reproductive stages of indigenous ewe.