• 한국철도학회논문집
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• 제11권5호
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• pp.489-498
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• 2008

일반적으로 허용처짐 기준은 정적 사용성과 구조적안전성에 그 기반을 두고 있으며 진동사용성에 대한 고려는 부족하다. 따라서 진동사용성을 고려할 수 있는 교량의 허용처짐 기준이 필요하다고 판단된다. 본 논문에서는, 한국철도교설계기준의 허용처짐기준을 주파수영역의 진동사용성 기준과 비교하였으며, 프랑스 및 일본의 철도교설계기준의 진동사용성 허용기준 또한 분석하였다. 그 결과, 한국철도교설계기준의 경우 열차의 속도에 따른 기준으로 진동지속시간이 부분적으로 고려되었지만 진동사용성은 만족하지 못하고 있는 것으로 판단되었으며, 국외 철도교설계기준비 분석결과, 진동사용성을 고려한 허용처짐기준을 제시할 수 있을 것으로 판단하였다. 공용중인 철도교량의 진동사용성을 평가하기위하여 다양한 형식의 철도교량의 진동신호를 측정하였다. 그리고, 현장에서 적용하기 편리한 진동사용성 처짐 및 강성한계를 제시하기 위하여 공용중인 교량을 대상으로 차량 교량 상호작용해석을 수행하였다.

• 한국동물생명공학회지
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• 제34권1호
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• pp.2-9
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• 2019

Because mesenchymal stem cells (MSCs) maintain distinct capacities with respect to self-renewal, differentiation ability and immunomodulatory function, they have been highly considered as the therapeutic agents for cell-based clinical application. Of particular, differentiation condition alters characteristics of MSCs, including cellular morphology, expression of gene/protein and cell surface molecule, immunological property and apoptosis. However, the previous results for differentiation-related apoptosis in MSCs have still remained controversial due to varied outcomes. Therefore, the present study aimed to disclose periodical alterations of pro- and anti-apoptosis in MSCs under differentiation inductions. The human dental pulp-derived MSCs (DP-MSCs) were differentiated into adipocytes and osteoblasts during early (1 week), middle (2 weeks) and late (3 weeks) stages, and were investigated on their apoptosis-related changes by Annexin V assay, qRT-PCR and western blotting. The ratio of apoptotic cell population was significantly (p < 0.05) elevated during the early to middle stages of differentiations but recovered up to the similar level of undifferentiated state at the late stage of differentiation. In the expression of mRNA and protein, whereas expressions of pro-apoptosis-related makers (BAX and BAK) were not altered in any kind and duration of differentiation inductions, anti-apoptosis marker (BCL2) was significantly (p < 0.05) elevated even at the early stage of differentiations. The recovery of apoptotic cell population at the late stage of differentiation is expected to be associated with the response by elevation of anti-apoptotic molecules. The present study may contribute on understanding for cellular mechanism in differentiation of MSCs and provide background data in clinical application of MSCs in the animal biotechnology to develop effective and safe therapeutic strategy.

• 한국재료학회지
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• 제29권4호
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• pp.241-251
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• 2019

There are several manufacturing techniques for developing thermionic cathodes for vacuum ultraviolet(VUV) ionizers. The triple alkaline earth metal emitters(Ca-Sr-Ba) are formulated as efficient and reliable thermo-electron sources with a great many different compositions for the ionizing devices. We prepare two basic suspensions with different compositions: calcium, strontium and barium. After evaluating the electron-emitting performance for europium, gadolinium, and yttrium-based cathodes mixed with these suspensions, we selected the yttrium for its better performance. Next, another transition metal indium and a lanthanide metal neodymium salt is introduced to two base emitters. These final composite metal emitters are coated on the tungsten filament and then activated to the oxide cathodes by an intentionally programmed calcination process under an ultra-high vacuum(${\sim}10^{-6}torr$). The performance of electron emission of the cathodes is characterized by their anode currents with respect to the addition of each element, In and Nd, and their concentration of cathodes. Compared to both the base cathodes, the electron emission performance of the cathodes containing indium and neodymium decreases. The anode current of the Nd cathode is more markedly degraded than that with In.

• 한국구조물진단유지관리공학회 논문집
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• 제25권2호
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• pp.35-42
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• 2021

본 연구에서는 NFPA 13을 참조하여 구조물 2층을 구현한 강재지그를 제작하였으며, 그루브 조인트의 배관연결재가 적용된 배관계통을 구성하고 정적 반복가력에 의한 지진모사실험을 수행하였다. 지진모사실험은 엑츄에이터로 건축물 내진설계기준의 최대허용 층간변위에 대한 반복가력실험을 수행하였다. 지진하중 발생 시 입상배관의 구조물에 대한 변형 또는 구조부재간의 상대변위에 의한 변위지배적인 거동에 따른 배관계통과 주요 배관요소의 지진거동을 분석하였다. 배관계통의 변형각은 기존의 센서를 이용하여 측정하기가 어려우므로 이미지측정시스템을 적용하였다.

• 한국구조물진단유지관리공학회 논문집
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• 제25권1호
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• pp.112-119
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• 2021

지진하중으로 인한 배관계통의 파괴모드는 라체트를 동반하는 저주기 피로파괴이며 비선형 거동이 집중되고 파손이 발생하는 요소는 엘보인 것으로 나타났다. 본 연구에서는 저주기 피로에 의한 SCH 40 3인치 탄소강관엘보의 파괴기준을 정량적으로 표현하기 위하여 한계상태를 누수로 정의하고 면내반복가력실험을 수행하였다. 배관계통에서 지진하중에 취약한 요소인 탄소강관엘보에 대하여 모멘트-변형각의 관계를 이용한 손상지수를 나타내었으며 힘-변위의 관계를 이용하여 산정된 손상지수와 비교-분석하였다. 탄소강관엘보에 대하여 반복되는 외력에 의한 소산에너지에 기반을 둔 손상지수로서 누수가 발생한 한계상태를 정량적으로 표현하였다.

• 한국동물위생학회지
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• 제45권4호
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• pp.305-316
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• 2022

Bordetella (B.) bronchiseptica, Mycoplasma (M.) felis, and Chlamydia (C.) felis are considered as main bacterial pathogens of feline upper respiratory tract disease (URTD). In this study, a new triplex quantitative real-time polymerase chain reaction (tqPCR) assay was developed for the rapid and differential detection of these bacteria in a single reaction. The assay specifically amplified three bacterial genes with the detection limit of below 10 copies/reaction. The assay showed high repeatability and reproducibility, with coefficients of intra-assay and inter-assay variation of less than 1%. Based on the diagnostic results of the assay using 94 clinical samples obtained from cats with URTD signs, prevalence of B. bronchiseptica, M. felis, or C. felis was 10.6%, 36.2%, or 6.4%, respectively, indicating that the diagnostic sensitivity was comparable to those of previously reported monoplex qPCR assays. The dual infection rates for B. bronchiseptica and M. felis or M. felis and C. felis was 2.1% or 3.2%, respectively. These results indicated that M. felis has been widely spread, and its co-infection with B. bronchiseptica or M. felis has been frequently occurred in Korean cat population. The developed tqPCR assay will serve as a promising tool for etiological and epidemiological studies of these three bacterial pathogens and the prevalence data obtained in this study will contribute to expanding knowledge about the epidemiology of feline URTD in Korea.

• 한국구조물진단유지관리공학회 논문집
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• 제12권2호
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• pp.121-130
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• 2008

최근 한국에서는 기상이변 및 노후화, 지진발생 등에 따른 댐 손상 및 붕괴 사례가 증가하고 있으며, 이와관련된 댐안전 문제는 국가차원의 재난관리 측면에서 중요하게 다루어지고 있다. 지속적인 댐의 안전성 확보를 위해 정부차원의 다각적 관련 법제도 마련뿐 아니라, 각 댐 관리기관들에서도 댐의 안전관리를 위한 다양한 제도적, 기술적 대책을 수립하고 있다. 본 연구에서는 기존 댐 안전관리 업무의 모순점을 개선하고, 지속적이며 효율적인 댐 안전관리 업무수행을 위해 댐안전관리시스템(KDSMS)을 개발하였다. 댐안전관리시스템은 제원정보시스템 및 수문정보시스템, 현장점검시스템, 지진감시 등을 포함한 매설계기계측시스템, 조사진단시스템, 총괄 정보자료시스템 등으로구성되어 있다. 댐안전관리시스템은 현장 및 본사의 안전관리관련의 세부 정보뿐 아니라, 현장 기술자 및 본사, 연구소 등 관련자들의 안전관리 업무내용 및 상호연계를 총괄하고 있는 전사적 시스템으로 개발되었다.

• 한국환경복원기술학회지
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• 제27권4호
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• pp.15-27
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• 2024

Ecosystem-disturbing plant species pose a significant threat to native ecosystems due to their high reproductive capacity, making it essential to monitor their distribution and develop effective mitigation strategies. Consequently, it is crucial to enhance the evaluation of the impacts of these species in environmental impact assessments by incorporating scientific evidence alongside qualitative assessments. This study introduces a dispersal model into the species distribution model to simulate the potential spread of ecosystem-disturbing plant species, reflecting their ecological characteristics. Additionally, we developed mitigation scenarios and quantitatively calculated reduction rates to propose effective mitigation strategies. The species distribution model showed a reliable AUC (Area Under the Curve) of at least 0.890. The dispersal model's results were also credible, with 31 out of 34 validation coordinates falling within the predicted spread range. Simulating the impact of the spread of ecosystem-disturbing plant species over the next five years revealed that one project site had potential habitats for Ambrosia artemisiifolia, necessitating robust mitigation measures such as seed removal. Another project site, with potential habitats for Symphyotrichum pilosum, indicated that physical removal methods within the site were effective due to the species' relatively short dispersal distance. These findings can serve as fundamental data for project executors and reviewers in evaluating the impact of the spread of ecosystem-disturbing plant species during the planning stages of projects.

• 한국수산과학회지
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• 제46권5호
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• pp.552-558
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• 2013

This study was performed to determine effects of the supplementation of Kimchi lactic acid bacterial culture in extruded pellets (EP) on the growth, body composition, blood chemistry and immune response of olive flounder Paralichthys olivaceus. Four hundred eighty individuals averaging 16.1 g were randomly distributed into 12, 180 L flow-through tanks (forty fish per tank). Four concentrations of Kimchi lactic acid bacterial culture (KL) were prepared: Control (0%), 0.1%, 0.2% and 0.5%. Three concentrations (0.1%, 0.2% and 0.5%) of Kimchi lactic acid bacterial culture were each diluted to 10% of EP weight and then fully absorbed by EP for 10 minutes. Each diet was fed to triplicate groups of fish. Fish were hand-fed to apparent satiation twice a day for 8 weeks. At the end of the 8-week feeding trial, the plasma lysozyme and bacterial activities of fish were determined. In addition, the cumulative mortality of fish was monitored for 8 days after their artificial infection with Edwardsiella tarda. The weight gain, specific growth rate, feed efficiency ratio, protein efficiency ratio, protein retention, hepatosomatic index and condition factor of fish were not affected by dietary supplementation with KL. None of the proximate composition, plasma parameters, lysozyme or bactericidal activities of fish was affected by dietary supplementation with KL. However, the cumulative mortalities of fish fed EP containing 0.1% and 0.5% Kimchi lactic acid bacterial culture were relatively low compared to that of fish fed the control diet. In conclusion, dietary supplementation with KL did not effectively improve growth, feed utilization, body composition, plasma chemistry, lysozyme, bactericidal activities or immune response of olive flounder after E. tarda infection under these experimental conditions.

• 한국수정란이식학회지
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• 제29권3호
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• pp.283-287
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• 2014

Sexed semen is commonly used for the production of calves of the desired gender. Gender selection is important in animal production industries. For example, female cattle are required for the dairy industry while males are preferred in the beef cattle industry. The present study was to assess the in vivo embryo production efficiency using the semen separated according to sex during superovulation in Hanwoo. Seventy Hanwoo donor cows were flushed on day 7 of estrus cycle with same FSH and artificial insemination by the same technicians. Embryos were recovered on 7 days after the third insemination by flushing the uterus with embryo collection medium. KPN semen straws used artificial insemination contained 20 million sperm (total number 60 million per donor). Sex-sorted semen straws contained 4 million sperm (total number 12 million per donor). The results obtained were as follows: No differences were observed in the efficiency of superovulation rates on KPN semen 87%, and sexed semen 100%, respectively. The mean numbers of total embryos are each $12.58{\pm}8.31$ and $13.25{\pm}7.86$. The mean numbers of transferable embryos, sexed semen were significantly lower than KPN semen ($3.75{\pm}1.98$ vs. $8.23{\pm}6.07$, P<0.05). The rates of unfertilized embryos from superovulation using sexed semen were significantly higher than KPN semen (50% vs. 15%, P<0.05). The rate of degenerated 2-cell embryos from sexed and KPN semen was 60.87% and 11.11%, respectively (p<0.05). In conclusion, these results indicate that superovulation using sexed semen was useful, but efficient embryo production was important to reducing the damage caused by the Flowcytometer-based sperm sorting procedure.