• 제목/요약/키워드: Jeju orange

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Evaluation of Shiranuhi, a Hybrid of Kiyomi Tangor and Nakano No.3 Ponkan, for Resistance to Citrus Canker in Growth Chamber

  • Myung, Inn-Shik;Hyun, Jae-Wook;Kim, Kwang-Sik;Lee, Seong-Chan;Lim, Han-Cheol
    • The Plant Pathology Journal
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    • 제19권5호
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    • pp.253-256
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    • 2003
  • Citrus canker disease is caused by bacteria Xanthomonas axonopodis .pv. Citri. Shiranuhi cultivar, a hybrid of Kiyomi tangor and Nakano No.3 ponkan was evaluated for resistance to citrus canker based on initiation of disease, percent area of lesion infected and growth rate of bacteria in the leaf under growth chamber condition. Significant differences between susceptible plant and resistant plants were observed in these assays. Resistant plants showed delayed disease symptoms compared to the susceptible plants after spray inoculation of the pathogen. The resistant verities, satsuma, yuzu, and Shiranuhi showed symptoms after six days where as susceptible, mexican lime showed the symptoms just after three days of inoculation. 18 days after inoculation, percent area of lesions developed on leaf and disease severity differed significantly in susceptible and resistant plants, and were ranked as follows: mexican lime > early satsuma =Shiranuhi =yuzu (P <, 0.01). However, 30 days after inoculation, percent area of lesion was further differentiated into resistant and highly resistant plants. That was ranked as follows: sweet orange> early satsuma =Shiranuhi =Kiyomi > yuzu (P < 0.01). These results indicate that host reaction to the bacterial was more distinct when the disease developed for a longer period. Growth rates of a citrus canker bacterium during 16 40 h also were distinct after infiltration into leaves of susceptible and resistant plants, and were ranked as follows: sweet orange> early satsuma =Shiranuhi =Kiyomi =yuzu (P < 0.01). Based on these results, we concluded that Shiranuhi is resistant to citrus canker as compared to Kiyomi, early satsuma, and yuzu.

성분분류학적 방법에 의한 제주도산 감귤류 과피의 생약학적 연구 (The Pharmacognosical Study on the Citrus Fruit Peels cultivated in Jeju Island by Chemotaxonomy)

  • 김창민;백완숙;권용수;고경수;허인옥
    • 생약학회지
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    • 제49권2호
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    • pp.113-123
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    • 2018
  • The application of 112 samples of Citrus fruit peel, which is commonly cultivated in Korea as crude drug, has been limited due to the absence of criteria concerning chemical constituents and application of itself. In the context, this study is on the analysis of 11 flavonoid compounds. As a result, the flavonoid pattern could be classified into 5 groups. Furthermore, by utilizing this HPLC data, this study could provide a basis for fruit peel applications of not only Citrus unshiu, but also its cultivars, hybrids and some of Citrus plants which has been cultivated in Jeju Island for a long time. Moondan could be used for the same purposes as Chinese Huajuhong, and Poncirus trifoliata of Korean Jisil origin are effective enough, in terms of ingredients and function, compared with C. aurantium. Based on the content and composition of the PMF region in this study, C. unshiu and C. reticulata, as Tanaka System shows, should be considered as different hortcultural speicies. Also, Jikak, which is known as 'sour orange' until now, is confirmed as Chosen-daidai, C. tenuissima, that was reported by Tanaka in 1970, and this C. tenuissima could be used for the same purposes as sour orange.

Evaluation of Potential of Mandarin Hybrid 'Shiranuhi' against inoculation of Bacterial Canker Disease Pathogen (Xanthomonas axonopodis pv. citri) in Citrus Field in Jeju Island

  • Hyun, Jae-Wook;Myung, Inn-Shik;Lee, Seong-Chan;Kim, Kwang-Sik;Lim, Han-Cheol
    • The Plant Pathology Journal
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    • 제19권5호
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    • pp.248-252
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    • 2003
  • This study was carried out to evaluate potential of Shiranuhi cultivar against inoculation of causal pathogen of citrus canker, Xanthomonas axonopodis pv. citri in Jeju Island by comparing degree of susceptibility of fruits and leaves/twigs, and analysis of incidence of canker disease. Progression of symptom, disease incidence, and percent area of lesion were surveyed for evaluation of resistance. In greenhouse condition, symptoms of bacterial citrus canker progressed more rapidly in sweet orange, a moderately susceptible cultivar, than in other four cultivars (satsuma mandarin, 'Kiyomi', 'Shiranuhi' and 'Yuzu'). At 20 days after inoculation, disease severity was the highest in sweet orange (5.0$\pm$0.0), and all tested leaves were distorted or had dropped. On the other hand, 'Yuzu' showed the lowest disease severity (2.6$\pm$0.47), followed by 'Kiyomi' (4.0$\pm$0.0), 'Shiranuhi' (4.0$\pm$0.82), and satsuma mandarin (4.3$\pm$0.47). Percent area of lesion per leaf 30 days after inoculation was the highest in sweet orange (8.31$\pm$1.78), followed by satsuma mandarin (1.51$\pm$1.25), 'Shiranuhi' (1.39$\pm$0.94), and 'Kiyomi' (1.1$\pm$0.9), while the lowest was in 'Yuzu' (0.26$\pm$0.17). Infield condition, percentage of diseased leaf in 'Shiranuhi' was very low, 5.2$\pm$2.9, compared with sweet orange, 71.0$\pm$ 11.5, while that of satsuma mandarin and 'Kiyomi' were 6.9$\pm$7.0 and 4.3$\pm$2.0, respectively. Percentages of diseased leaf was higher (17.4$\pm$7.1) than that of diseased fruit (3.2$\pm$2.5) in severely diseased trees of Shiranuhi cultivar, and the disease was not observed on twig in open field condition. Lesion sizes on leaves and fruits in open field condition were 4.1$\pm$2.2 mm2 and 5.1$\pm$5.6 mm2, respectively, while those in greenhouse condition were 8.7$\pm$5.7 mm2, 10.4$\pm$9.2 mm2 and 5.6$\pm$2.6 mm2 on leaves, fruits and twigs, respectively. The disease was observed in 5.6% out of total 107 farmers Shiranuhi fields under polyethylene film house, and average percentages of diseased tree in 31 fields of Shiranuhi cultivar and adjacent satsuma mandarin fields were 0.02% and 14.8%, respectively. Average percentage of diseased fruit was 1.6% in satsuma mandarin which was not observed in anyone of all the 31 Shiranuhi farmers fields. Therefore, it was concluded that 'Shiranuhi' cultivar is not potential against causal pathogen of citrus canker disease in Jeju Island because the cultivar has similar resistance as satsuma mandarin which occupies over 95% of total 25,000 ha in Jeju Island in polyethylene film houses protected from outside.

동질 사배체 탱자에서 염색체 배가와 수체 표현형의 변이 (Chromosome Redundancy and Tree Phenotype Variation in Autotetraploid Trifoliate Orange)

  • 오은의;채치원;김샛별;;윤수현;고상욱;송관정
    • 원예과학기술지
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    • 제32권3호
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    • pp.366-374
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    • 2014
  • 이배체 탱자로부터 자연적으로 발생한 동질 사배체 탱자의 수체 형질 관련 표현형 및 유전체 메틸화 변이 정도를 분석하여 후성 유전의 하나인 유전체 메틸화가 동질 사배체의 표현형 변이의 요인으로 작용할 수 있음을 구명하고자 본 실험을 수행하였다. 이배체 탱자에서 유래된 14주의 사배체 탱자로부터 염색체를 분석하여 이수성이 없는 2n = 4X = 36 식물체로 확인하였다. CMA 핵형 분석 결과 염색체가 배가된 동질 사배체임을 확인할 수 있었다. 동질 사배체에서 수고, 수폭, 원가지 수, 원가지 길이, 분지 각도, 마디 길이, 잎의 특성 등 동질 사배체 수체 표현형에 있어서 상당한 변이가 나타남을 확인하였다. 또한 동질 사배체 광합성률에는 큰 차이는 없었지만, SPAD 값에 의한 엽록소 지수에 있어서도 표현형이 다양하게 나타나는 것을 알 수 있었다. 그 외에도 기공 밀도와 공변 세포 길이에 있어서 광범위하게 변이가 관찰되는 것을 알 수 있었다. Global cytosine DNA 메틸화를 분석한 결과 개체 간 메틸화 정도에 차이가 존재함을 알 수 있었다. 동질 사배체 탱자 14주의 절반이 이배체 탱자의 메틸화와 비교하였을 때 2배 이상으로 나타난 것을 확인하였다. 본 연구 결과 동질 사배체에서 나타나는 수체형질 변이가 gene redundency를 줄이기 위한 global cytosine DNA 메틸화와 관계될 수 있음을 확인하였다.

Studies on Physiology, Ecology and Protection of Citrus Canker Caused by Xanthomonas axonopodis pv, citri

  • Lee, Seong-Chan;Hyun, Jae-Wook;Kim, Dong-Hwan;Kim, Kwang-Sik;Lim, Han-Chul
    • 한국식물병리학회:학술대회논문집
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    • 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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    • pp.124-124
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    • 2003
  • Citrus canker is very important disease in international trade of citrus. The disease was usually take place from late of June, and severe middle of July to middle of August, though disease occurrence was affected by environmental conditions. In pathogenicity test, three varieties, orange, lemon and kiyomi among 7 varieties, were succeptible, two varieties, satsuma mandarin and iwasachi, intermediate resistant. On the other hand, shiranuhi and yuzu were resistant relatively. The pathogen, Xanthomonas axonopodis pv. citri, grew well in PD broth adjusted to pH 7.0 at 26$^{\circ}C$. It's growth was best in medium containing group of monosaccharide as a carbon source and group of ammonium as a nitrogen source. Tow isolates were resistant to streptomycin among 11 isolates isolated from diseased leaves in field in Jeju-Do. The streptomycin sensitives isolate was controlled by in greenhouse test. On the other hand, the resistant and sensitive isolates were controlled by treatment with copper sulfate, the control value is 88.7% and 90.6%, respectively.

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Reduced Autophagy in 5-Fluorouracil Resistant Colon Cancer Cells

  • Yao, Cheng Wen;Kang, Kyoung Ah;Piao, Mei Jing;Ryu, Yea Seong;Fernando, Pattage Madushan Dilhara Jayatissa;Oh, Min Chang;Park, Jeong Eon;Shilnikova, Kristina;Na, Soo-Young;Jeong, Seung Uk;Boo, Sun-Jin;Hyun, Jin Won
    • Biomolecules & Therapeutics
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    • 제25권3호
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    • pp.315-320
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    • 2017
  • We investigated the role of autophagy in SNUC5/5-FUR, 5-fluorouracil (5-FU) resistant SNUC5 colon cancer cells. SNUC5/5-FUR cells exhibited low level of autophagy, as determined by light microscopy, confocal microscopy, and flow cytometry following acridine orange staining, and the decreased level of GFP-LC3 puncta. In addition, expression of critical autophagic proteins such as Atg5, Beclin-1 and LC3-II and autophagic flux was diminished in SNUC5/5-FUR cells. Whereas production of reactive oxygen species (ROS) was significantly elevated in SNUC5/5-FUR cells, treatment with the ROS inhibitor N-acetyl cysteine further reduced the level of autophagy. Taken together, these results indicate that decreased autophagy is linked to 5-FU resistance in SNUC5 colon cancer cells.

지하공기 이용 히트펌프시스템의 망고온실 난방효과 (Heating Effect of Greenhouse Cultivated Mangos by Heat Pump System using Underground Air as Heat Source)

  • 강연구;김영화;유영선;김종구;장재경;이형모
    • 한국신재생에너지학회:학술대회논문집
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    • 한국신재생에너지학회 2011년도 춘계학술대회 초록집
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    • pp.200.1-200.1
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    • 2011
  • Underground air is a special energy source in Jeju and distributes lava cave, pyroclastic, open joint, and crushing zone. A possible area to utilize underground air is 85% of Jeju except to the nearby area of Sambang Mt. and 25m high coastal area from sea level. In Jeju, underground air is used for heating agricultural facilities such as greenhouse cultivated mangos, Hallbong and mandarin orange, pigsty, mushroom cultivation house, etc. and fertilizing natural $CO_2$ gas by suppling directly into agricultural facilities. But this heating method causes several problem because the underground air has over 90% relative humidity and is inadequate in heating for crops. Mangos are the most widely grown tropical fruit trees and have been cultivated since 1993 in Jeju. In Jeju, the cultivating area is about 20ha and amount of harvest is 275ton/year in 2010. In this study, the heat pump system using underground air as heat source was installed in mangos greenhouse which area is $495m^2$. The capacity of heat pump system and heat storage tank was 10RT, 5ton respectively and heating effect and heating performance of the system were analysed.

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Phytophthora-Induced Diseases on Citrus in Jeju Island

  • Hyun, Jae-Wook;Lee, Seong-Chan;Kim, Kwang-Sik;Jee, Hyeong-Jin
    • The Plant Pathology Journal
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    • 제17권3호
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    • pp.184-188
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    • 2001
  • Phytophthora-induced diseases on citrus in Jeju island have been considered of minor importance because of the use as root stock of trifoliate orange, which is immune to Phytophthora. However, brown rot on fruit, which severely occurred in 1998 and 1999, has become a great threat to citrus production in the island. About one-half of the surveyed orchards were infected in 1998 and 4 out of 19 infected fields showed over 20% fruit infection rate. The disease was less severe in 1999, with an estimated infected area and total fruit reduction of 3,155 ha and 15,300 tons, respectively. Typical gummosis was also occasionally observed on cv. Shiranugi, which is mostly cultivated under plastic film houses. Two types of Phytophthora were consistently isolated from various plant parts, identified as P. citrophthora and P. nicotianae. The former was isolated from the aerial parts of the fruit, young leaf, and shoot in the fields. Meanwhile, the latter was only isolated from the basal stem showing gummosis in plastic film houses.

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온주밀감 신품종 '하례조생' 육성 (A New Early Maturing Satsuma Mandarin Cultivar, 'Haryejosaeng')

  • 윤수현;김성종;김한용;박재호;안현주;강성구;문영일;김광식;이동훈;고상욱;김창명
    • 한국육종학회지
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    • 제40권2호
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    • pp.184-187
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    • 2008
  • A new early maturing satsuma mandarin (Citrus unshiu Marc.) 'Haryejosaeng' was developed as a nucellar seedling selection of 'Tachima Wase' (C. unshiu Marc.) that was crossed with C. natsudaidai Hayata at the National Institute of Subtropical Agriculture in Jeju island in 1992. The 'Jegam ga No. 2', a first selection from the seedlings fruited in 2001 was finally named "Haryejosaeng" through field evaluation trials at three locations in Jeju island from 2003 to 2004. 'Haryejosaeng' produces seedless fruit maturing in early November, and has higher soluble solids and lower acidity than 'Miyagawa Wase', the leading early-maturing satsuma mandarin cultivar in Jeju island. Fruit weight is moderate at about 80~90 g and its shape is compressed-oblate globose with a light orange color. The rind thickness of about 2 mm provides easy peeling. The flesh shows light orange colored and contains 10 to 11 Brix and 1 to 1.1% acidity when mature. Tree shows vigorous growth and spreading thornless twigs. Alternate bearing is similar to 'Miyagawa Wase'. 'Haryejosaeng' is susceptible to citrus scab disease and melanose, but resistant to citrus canker.

시설재배 '부지화' M16 A계통의 '탱자'와 '스윙글 시트루멜로' 대목과 과실 및 잎의 수분상태가 과실품질 차이에 미치는 영향 (Effect of Water States of Fruit Vesicle and Leaf on Fruit Quality in 'Trifoliate' Orange and 'Swingle citrumelo' Rootstock of 'Shiranuhi' [(Citrus unshiu ${\times}$ C. sinensis) ${\times}$ C. reticulata] Mandarin Hybrid, M16 A Line in Plastic Film House Cultivation)

  • 한상헌;강종훈
    • 생물환경조절학회지
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    • 제20권3호
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    • pp.204-210
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    • 2011
  • 본 연구에서는 M16 A계통 '부지화'를 강세대목인 대목 '스윙글 시트루멜로' 또는 보통대목인 '탱자'에 접목하여 무가온 하우스에서 재배한 잎과 과실 사양의 수분상태 변화 및 과실 성숙기의 과실 가용성 고형물과 산 함량변화를 조사했다. 강세대목인 '스윙글 시트 루멜로'와 보통대목인 '탱자'에 접목한 M16 A계통 '부지화'의 대목에 따라 과실의 품질은 영향을 받는 것으로 나타났는데, '스윙글 시트루멜로' 대목은 '탱자' 대목보다 과실 가용성 고형물과 산함량이 낮았다. 동트기 직전에 측정된 과실사양의 수분포텐셜 및 팽압은 '스윙글 시트루멜로' 대목이 '탱자' 대목보다 조금 높은 경향을 보였으나, 삼투 포텐셜의 값은 '탱자' 대목이 '스윙글 시트루멜로' 대목보다 낮았다. 과실사양과 같은 시간대에 측정된 엽수분포텐셜 값의 변화도 과실 사양의 삼투포텐셜 변화와 유사한 경향을 보여주었다. 이상의 결과로 보아, M16 A계통 '부지화'에 있어 두개의 대목에 따라 잎의 수분 상태와 과실사양의 삼투포텐셜 차이가 있었으며, 이러한 차이로 인하여 과실 가용성 고형물과 산 함량에 차이가 나타나는 것으로 판단되며, 대목에 따른 수분 상태와 과실 품질의 차이는 대목의 뿌리분포가 영향을 미쳤기 때문인 것으로 사료된다.