• Journal of Microbiology and Biotechnology
• /
• v.18 no.3
• /
• pp.503-511
• /
• 2008

An edible marine red alga, Grateloupia filicina, collected from Jeju Island of Korea was hydrolyzed by cheap food-grade carbohydrases (Viscozyme, Celuclast, AMC, Termamyl, and Ultraflo) to investigate its anticoagulant activity. Among the tested enzymatic extracts of G. filicina, a Termamyl extract showed the highest anticoagulant activity. Anion-exchange chromatography on DEAE-cellulose and gel-permeation chromatography on Sepharose-4B were used to purify the active polysaccharide from the crude polysaccharide fraction of G. filicina. The purified sulfated polysaccharide (0.42 sulfate/total sugar) showed ${\sim}1,357kDa$ molecular mass and was comprised mainly of galactose(98%) and 1-2% of glucose. The sample showed potential anticoagulant activity on activated partial thromboplastin time (APTT) thrombin time (TT) assays. The purified G. filicina anticoagulant (GFA) inhibited the coagulation factor X (92%), factor II (82%), and factor VII (68%) of the coagulation cascade, and the molecular interaction (protein-polysaccharide) was highly enhanced in the presence of ATIII (antithrombin III). The dissociation constant of polysaccharide towards serine proteins decreased in the order of FXa (58.9 nM) >FIIa (74.6 nM) >FVII (109.3 nM). The low/less cytotoxicity of the polysaccharide benefits its use in the pharmaceutical industry; however, further studies that would help us to elucidate the mechanism of its activity are needed.

• KSCE Journal of Civil and Environmental Engineering Research
• /
• v.34 no.5
• /
• pp.1599-1608
• /
• 2014

This study is to suggest specialty contractors with countermeasures to construction orders falling off, one of the construction business indicators for the construction industry, by analyzing the impact of dwindling construction orders to business performance indicators of specialty contractors as well as identifying inter-relationships between those performance indicators. For specialty contractors of civil construction, it is analyzed that their current ratio is significantly affecting obtention of construction orders, which in turn greatly affecting the ratio of owner's equity. It seems that the amount of construction orders has a direct relationship with the corporate stability. Therefore, this type of specialty contractors are determined to be able to obtain more orders for construction by improving current ratio.

• The Plant Pathology Journal
• /
• v.31 no.3
• /
• pp.269-277
• /
• 2015

Anthracnose caused by Colletotrichum gloeosporioides has been destructive during pepper fruit production in outdoor fields in Korea. In vitro antifungal activities of 15 different plant essential oils or its components were evaluated during conidial germination and mycelial growth of C. gloeosporioides. In vitro conidial germination was most drastically inhibited by vapour treatments with carvacrol, cinnamon oil, trans-cinnamaldehyde, citral, p-cymene and linalool. Inhibition of the mycelial growth by indirect vapour treatment with essential oils was also demonstrated compared with untreated control. Carvacrol, cinnamon oil, trans-cinnamaldehyde, citral and eugenol were among the most inhibitory plant essential oils by the indirect antifungal efficacies. Plant protection efficacies of the plant essential oils were demonstrated by reduced lesion diameter on the C. gloeosporioides-inoculated immature green pepper fruits compared to the inoculated control fruits without any plant essential oil treatment. In planta test showed that all plant essential oils tested in this study demonstrated plant protection efficacies against pepper fruit anthracnose with similar levels. Thus, application of different plant essential oils can be used for ecofriendly disease management of anthracnose during pepper fruit production.

• KSCE Journal of Civil and Environmental Engineering Research
• /
• v.33 no.6
• /
• pp.2563-2573
• /
• 2013

Specialty contractors are in charge of the actual construction work and managing many work force in construction sites. The effective educational program for specialty contractors' employees is essential to improve the competitiveness of construction industry. However, there is no in-depth research on training programs for their management or technical staff. Therefore, this study proposes a framework of educational program for the specialty contractors' employees in order to enhance their competence. An extended review of literature related the educational program was carried out to understand the concept, structure, and element of the education system. A questionnaire survey for specialty contractors' employees was performed to investigate the status and problems of educational programs as well as the their educational needs. Based on the survey, the demand-oriented education programs is required to meet the needs of specialty contractors' employees. The framework of demand-oriented education programs for specialty contractors' employees consists of training contents, training type and method, training hour.

• Journal of Industrial Convergence
• /
• v.18 no.3
• /
• pp.109-116
• /
• 2020

The purpose of this study is to make suggestions for the infectious disease surveillance systems as part of the government's healthcare policy intended to minimize damage by implementing an appropriate and swift crisis management in the event of future infectious disease outbreaks. To that end, this descriptive study analyzes the infectious disease outbreaks and the management and control thereof in Korea and other countries, so as to suggest some approaches to infectious disease surveillance systems applicable to affected regions. The analysis results shed light on the causes of the spread of future infectious diseases over the past years, and the management systems that could possibly deal with the trial and error in the response policy. It seems crucial to roll out appropriate information, training and promotion programs as part of the national disaster response systems to prevent infectious diseases in relation to the roles of multiple relevant government agencies in the event of disasters especially amid the COVID-19 pandemic.

• Journal of Korean Society for Atmospheric Environment
• /
• v.20 no.5
• /
• pp.685-695
• /
• 2004

Size-segregated measurements of aerosol composition are used to estimate the transport of natural and anthropogenic aerosols at Gosan site during May 2002. The results of measurement show that not only soil dust but also anthropogenic aerosols, including sulfur and enriched trace metals such as Pb, Zn, Cu, are transported to Gosan. This study combines the size- and time-resolved aerosol composition measurements with factor analysis in order to identify some source materials. As a result, coarse particles (2.5${\mu}m$~12${\mu}m$) are influenced by soil, sea-salt, coal, coal combustion, and nonferrous sources. But fine particles have different sources. The fine particles, which the diameter is from 0.56${\mu}m$ to 2.5${\mu}m$, are more affected by road dust, oil combustion, industry. municipal incineration, and ferrous metal sources. The very fine particles, from 0.09${\mu}m$ to 0.56${\mu}m$, mainly supplied by biomass burning, oil combustion, nonferrous and ferrous metal sources.

• Journal of Environmental Science International
• /
• v.22 no.4
• /
• pp.481-492
• /
• 2013

In this study, the use survey of domestic and international weather information on coastal regions and the demand survey (e.g. general and in-depth surveys) for customer needs with coastal industries were carried out to design customized coastal weather contents. The general demand survey showed that most of the customers working in the coastal industries were interested in a short-term forecast, such as a general weather outlook (approximately 29% of the total respondents) and typhoon information (19%), and they preferred to be given the forecast information from new media such as the internet web-pages (36%) and mobile utilities (23%) rather than old media such as TV (16%) and radio (11%). In addition, only 31% of the total respondents were found to be satisfied with the use of the current coastal weather service. This low percentage might mainly be a result of lack of information accuracy (about 64%) and diversity (28%). From in-depth survey with site visiting, the need of coastal weather contents, such as weather elements, data form, a tool of communication, and forecast interval, differed with the working stages in three coastal industries (e.g. shipbuilding, maritime trade, and passenger transport industries).

• Applied Chemistry for Engineering
• /
• v.29 no.3
• /
• pp.264-269
• /
• 2018

PSf/D2EHPA/TBP/CNTs beads were prepared by immobilizing carbon nanotubes (CNTs) and two extractants, di-(2-ethylhexyl)-phosphoric acid (D2EHPA) and tributyl phosphate (TBP) on polysulfone (PSf). The prepared PSf/D2EHPA/TBP/CNTs beads were characterized by SEM, TGA, and FTIR. The removal rate of Sr(II) by PSf/D2EHPA/TBP/CNTs beads was well described by the pseudo-second-order kinetic model. The maximum removal capacity of Sr(II) obtained from Langmuir isotherm was found to be 5.52 mg/g. The results showed that the removal efficiency of Sr(II) by PSf/D2EHPA/CNTs beads prepared in this study was significantly improved compared to that of using PSf/D2EHPA/CNTs beads without TBP.

• Journal of fish pathology
• /
• v.23 no.1
• /
• pp.9-16
• /
• 2010

We determined the effects of temperature shifts on the kinetics of the numbers of antibody-secreting cell (ASC) in the olive flounder Paralichthys olivaceus immunised with formalin-killed Edwardsiella tarda. When fish that were acclimated to $22^{\circ}C$ and immunised at that temperature were transferred to a lower temperature ($12^{\circ}C$) at a various times (immediately, 1, 2 or 4 weeks) after immunisation, both further differentiation of B cells and secretion of antibody from the ASC developed at $22^{\circ}C$ were suppressed at $12^{\circ}C$. However, in the converse experiment ($12^{\circ}C$ to $22^{\circ}C$), the magnitude of the humoral immune response was recovered independent of the time of the transfer after immunisation at low temperature, even though the peak levels of each transferred group did not reach the level found in $22^{\circ}C$ control group. The results were confirmed by counting the number of specific antibody secreting cells (SASC) in the spleen. This study provides the evidences of the immune reaction that the potential for antibody production in B cells of flounder, the most important species in aquatic industry of Korea, immunized at high temperature is suppressed by subsequent exposure to low temperature and that low temperature-induced humoral immuno suppression can be reversed by exposure to a higher temperature.

• ALGAE
• /
• v.29 no.3
• /
• pp.237-247
• /
• 2014

Polysaccharides prepared from marine algae sometimes contain contaminants such as polyphenols and endotoxins that may mislead their bona fide biological activities. In this study, we examined bioactive contaminants in commercially available fucoindan from Fucus vesiculosus, along with ascophyllan and fucoidan from Ascophyllum nodosum. F. vesiculosus fucoidan inhibited the growth of Vibrio alginolyticus in a concentration-dependent manner ($0-1,000{\mu}g\;mL^{-1}$). However, the antibacterial activity of the fucoidan significantly reduced after methanol-extraction, and the methanol-extract showed a potent antibacterial activity. The extract also showed cytotoxicity to RAW264.7 and U937 cells, and induced apoptotic nuclear morphological changes in U937 cells. These results suggest that the antibacterial activity of the fucoidan is partly due to the methanol-extractable contaminants that can also contribute to the cytotoxicity on RAW264.7 and U937 cells. On the other hand, the activities to induce secretion of nitric oxide and tumor necrosis factor-${\alpha}$ from RAW264.7 cells were observed in the fucoidan even after methanol extraction, and the extract had no such activities. Our observations suggest that commercially available fucoidan should be purified prior to biochemical use.