• 약학회지
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• 제46권2호
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• pp.143-148
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• 2002

The human polyomavirus JC virus is the etiologic agent of progressive multifocal leukoencephalopathy (PML). The JC virus early promoter directs cell-specific expression of the viral replication factor large T antigen, thus transcriptional regulation constitutes a major mechanism of glial tropism in PML. Here we found that pentanucleotide sequence immediately upstream of the TATA sequence functions as a cell-specific silencer in the JC virus transcription. In vitro binding studies showed that synthetic oligonucleotides spanning a pentanucleotide sequence, designated "oligo 2", interacts with nuclear proteins from non-glial cells in a cell-specific manner. Furthermore, the sequence preferentially repressed the heterologous thymidine kinase promoter activity in non-glial cells. We also tested whether JC virus transcription is controlled by DNA methylation. Transient transfection of in vitro methylated JC virus promoter abolished transcription in both the glial and non-glial cells. The repression fold was much larger in glial cells than in non-glial cells. Taken together, this finding suggests that glial cell-specific expression of the JC virus is controlled by DNA methylation as well as cell-specific silencers.

• 한국유기농업학회지
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• 제30권3호
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• pp.393-407
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• 2022

본 논문에서는 Neobacillus sp. JC05의 추출물을 처리한 오이 식물체에서 고구마뿌리혹선충 감염에 대한 식물의 방어 반응을 조사하였다. Neobacillus sp. JC05의 추출물을 처리한 오이 식물체에서는 고구마뿌리혹선충에 의한 감염이 감소하여 control에 비하여 병 발생이 감소하였으며, 식물이 받는 피해도 줄어들었다. 이때 Neobacillus sp. JC05 추출물을 처리한 오이 식물체의 병 저항성 유전자의 발현량을 관찰한 결과, chitinase와 lipoxygenase 유전자의 발현량이 상대적으로 증가하였으며, 식물체 내 산화 스트레스를 감소시키는 항산화효소 중 peroxidase의 활성이 현저히 증가하는 것을 관찰하였다. 또한, Neobacillus sp. JC05 추출물을 처리한 오이 식물체의 토양 FDase 활성이 증가하였으며, 반면 인산가용화효소와 유레아제 활성은 control에 비해 유의하지 않았다. 그러므로, 이전의 연구 결과와 종합하면, Neobacillus sp. JC05의 추출물은 직접적으로 고구마뿌리혹선충의 알 부화율을 억제하고 유충의 사멸률을 높여 토양 내 고구마뿌리혹선충의 밀도를 감소시키기도 하지만 본 연구의 결과에 따르면, 오이 식물체의 고구마뿌리혹선충에 대한 방어 반응을 증가시켜 저항성을 유도하며, 이를 통해 고구마뿌리혹선충병 발생을 감소시키는데 기여하는 것으로 사료된다.

• Archives of Pharmacal Research
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• 제25권2호
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• pp.208-213
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• 2002

The human polyomavirus JC virus is the etiologic agent of progressive multifocal leukoencephalopathy (PML). As the JC virus early promoter directs cell-specific expression of the viral replication factor large T antigen, transcriptional regulation constitutes a major mechanism of glial tropism in PML. It has been demonstrated that SV4O or JC virus large T antigen interacts with p53 protein and regulates many viral and cellular genes. In this study we founts that p53 represses the JC virus early promoter in both glial and nonglial cells To identify the cis-regulatory elements responsible for p53-mediated repression, deletional and site-directed mutational analyses were performed . Deletion of the enhancer region diminished p53-mediated transcriptional repression. However, point mutations of several transcription factor binding sites in the basal promoter region did not produce any significant changes. In support of this observation, when the enhancer was fused to a heterologous promoter, p53 red reduced the promoter activity about three fold. These results indicate that the enhancer region is important for tole repression of JC virus transcription by p53. Furthermore, coexpression of JC virus T antigen with a p53 protein abolished p53-mediated repression of the JC virus early promoter in non-glial cells, but not in glial cells. This finding suggests that T antigen interacts with p53 and regulates JC virus transcription in a cell-specific manner.

• 미생물학회지
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• 제23권1호
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• pp.1-8
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• 1985

일산화 탄소를 이용한 enrichment 배양 방법을 통하여 흙으로부터 일산화탄소를 이용하여 성장 할 수 있는 세가지 종류 (JC1, JC2, HY1)의 호기성 Acinetobacter 들을 분리했다. 이세균들은 모두Gramdma성 세균들로 운동성이 없었으며, 지수성장기에는 간균의 형태를 나타내었으나 성장이 정지되었을 때에는 구균으로 변하였다. 이들은 페니실린에 대해 내성이 있었고 $42^{\circ}C$에서도 성장을 할 수 있었다. DNA의 G+C함량은 43%-44.5%이였고 모든 세균에서 oxidase의 활성이 나타나지 않았다. 이 세균들의 coloy들은 모두 둥글고 매끄러웠으며 연한 노란색을 띄었다. 이들은 또 여러가지 종류의 당이나 유기산, 아미노산. 알코올 등을 이용하여 생장할 수 있였다. JC1과 J JC2 벚 HY1이 30%의 일산화탄소를 이용하여 $30^{\circ}C$에서 성장할 때의 doubling time은 각각 19시간. 25시간, 그리 고 35시간 이었다. JC1의 자가영양적 성장을 위한 최적조건은 pH가 6.8이였L 온도는 $42^{\circ}C$ 그리고 일산화탄소의 농도는 30%이였다. JC1 의 자가영양적 성장에는 몰리브데늄이 필요치 않았고, 또 이 세균은 100ppm의 CO만으로도 성장할 수 있는 것으로 밝혀졌다.

• 한국초전도ㆍ저온공학회논문지
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• 제4권1호
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• pp.1-3
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• 2002

Single Josephson junctions, which are of cross type, of $50 ${\mu}{\textrm}{m}$ {\times} 50 ${\mu}{\textrm}{m}$$ were fabricated under several oxidation conditions to Investigate controllabilities of critical current density (Jc) with the standard KRISS processes. Considering that the self-field effect suppresses the observed critical current (Ice) at high Jc region, we could reasonably estimate Jc values from I-V observations. The dependence of the obtained Jc as a function of exposure, which is equal to pressure (P) times time (t), was well fitted to a curve of Jc ~ (Pt)-0.34. The maximum Jc value at the controllability margin was found to be 3 kA/cm$^2$with the current equipment set up.

• 한국발생생물학회지:발생과생식
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• 제21권3호
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• pp.237-247
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• 2017

Mammalian spermatogenesis occurs in a precise and coordinated manner in the seminiferous tubules. One of the attempts to understand the detailed biological process during mammalian spermatogenesis at the molecular level has been to identify the testis specific genes followed by study of the testicular expression pattern of the genes. From the subtracted cDNA library of rat testis prepared using representational difference analysis (RDA) method, a complimentary DNA clone encoding type III member of a DnaJ family protein, DnaJC18, was cloned (GenBank Accession No. DQ158861). The full-length DnaJC18 cDNA has the longest open reading frame of 357 amino acids. Tissue and developmental Northern blot analysis revealed that the DnaJC18 gene was expressed specifically in testis and began to express from postnatal week 4 testis, respectively. In situ hybridization studies showed that DnaJC18 mRNA was expressed only during the maturation stages of late pachytene, round and elongated spermatids of adult rat testis. Western blot analysis with DnaJC18 antibody revealed that 41.2 kDa DnaJC18 protein was detected only in adult testis. Immunohistochemistry study further confirmed that DnaJC18 protein, was expressed in developing germ cells and the result was in concert with the in situ hybridization result. Confocal microscopy with GFP tagged DnaJC18 protein revealed that it was localized in the cytoplasm of cells. Taken together, these results suggested that testis specific DnaJC18, a member of the type III DnaJ protein family, might play a role during germ cell maturation in adult rat testis.

• 한국유기농업학회지
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• 제12권1호
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• pp.101-114
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• 2004

This study was carried out to clarify the effects of antagonistic microorganism, Bacillus sp. JC181 isolated from the greenhouse soil grown cucumber plants on the growth inhibition of plant pathogen, fusarium wilt (Fusarium oxysporum) occurred in cucumber plants in greenhouse. Antagonistic bacterial strains were isolated and were investigated into the antifungal activity of the antagonistic microorganism against fusarium wilt. Screened fourteen bacterial strains which strongly inhibited F. oxysporum were isolated from thc greenhouse soil grown cucumber plants, and the best antagonistic bacterial strain designated as JC181, was finally selected. Antagonistic bacterial strain JC181 was identified to be the genus Bacillus sp. based on the morphological and biochemical characterization. Bacillus sp. JC181 showed 58.2% of antifungal activity against the plant pathogen growth of F. oxysporum. By the bacterialization of culture broth and heated filtrates of culture broth, Bacterial strain, Bacillus sp. JC181. showed 91.2% and 260% of antifungal activity against F. oxysporum, respectivrly.

• 한국식품영양학회지
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• 제13권2호
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• pp.139-145
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• 2000

To separate anticaries and antiinflammation from Aloe vera peel, we investigated a inhibited effect of Streptococcus mutans JC-2 that was antibiosis, glucosyltransferase activity about aloe-emodin and barbaloin. Aloe-emodin and barbaloin had strong antibiosis activity against Streptococcus mutans JC-2, they were especially antibiosis effect to low growth and prolong lag phase at attachment concentration 100$\mu\textrm{g}$/mL. The reduction rate of a culture fluid became to lessen than the comparison group for aloe-emodin and barbaloin. The intracellular materials of Streptococcus mutans JC-2 were to leakage as much as attachment concentration addition of aloe-emodin and barbaloin but there was no significant difference membrane demage between two active substances. The activity of GTase was inhibited by aloe-emodin and barbaloin and their inhibition rate was respectively 99.8%, 98.4% at the attachment concentration 100$\mu\textrm{g}$/mL.

• Restorative Dentistry and Endodontics
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• 제20권2호
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• pp.839-842
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• 1995

In its study, We investigated the effects of Areca catechu L on the growth and acid production of Streptococcus mutans JC-2 in broth system and the activity of glucosyltransferase. The results were summarized as follow; 1. The growth of Streptococcus mutans JC-2 was suppressed by adding Areca catechu L in broth system. Especially, its inhibitory effect was significant at 2,000ppm of concentration. 2. Areca catechu L decreased the acid production of Streptococcus mutans JC-2. Decrease of pH according to acid production was less in presence of Areca catechu L than in absence. 3. Areca catechu L exerted the inhibitory effect against glucosyltransferase activity form Streptococcus mutans JC-2.

• Korean Journal of Mathematics
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• 제22권1호
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• pp.91-121
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• 2014

Let X and Y be vector spaces. It is shown that a mapping $f:X{\rightarrow}Y$ satisfies the functional equation ${\ddag}$ $$2df(\frac{x_1+{\sum}_{j=2}^{2d}(-1)^jx_j}{2d})-2df(\frac{x_1+{\sum}_{j=2}^{2d}(-1)^{j+1}x_j}{2d})=2\sum_{j=2}^{2d}(-1)^jf(x_j)$$ if and only if the mapping $f:X{\rightarrow}Y$ is additive, and prove the Cauchy-Rassias stability of the functional equation (${\ddag}$) in Banach modules over a unital $C^*$-algebra, and in Poisson Banach modules over a unital Poisson $C^*$-algebra. Let $\mathcal{A}$ and $\mathcal{B}$ be unital $C^*$-algebras, Poisson $C^*$-algebras, Poisson $JC^*$-algebras or Lie $JC^*$-algebras. As an application, we show that every almost homomorphism $h:\mathcal{A}{\rightarrow}\mathcal{B}$ of $\mathcal{A}$ into $\mathcal{B}$ is a homomorphism when $h(d^nuy)=h(d^nu)h(y)$ or $h(d^nu{\circ}y)=h(d^nu){\circ}h(y)$ for all unitaries $u{\in}\mathcal{A}$, all $y{\in}\mathcal{A}$, and n = 0, 1, 2, ${\cdots}$. Moreover, we prove the Cauchy-Rassias stability of homomorphisms in $C^*$-algebras, Poisson $C^*$-algebras, Poisson $JC^*$-algebras or Lie $JC^*$-algebras, and of Lie $JC^*$-algebra derivations in Lie $JC^*$-algebras.