• Title/Summary/Keyword: Jang Hoon

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Immobilization stress increased cytochrome P450 1A2 (CYP1A2) expression in the ovary of rat

  • Hwang, Jong-Chan;Kim, Hwan-Deuk;Park, Byung-Joon;Jeon, Ryoung-Hoon;Baek, Su-Min;Lee, Seoung-Woo;Jang, Min;Bae, Seul-Gi;Yun, Sung-Ho;Park, Jin-Kyu;Kwon, Young-Sam;Kim, Seung-Joon;Lee, Won-Jae
    • Journal of Animal Reproduction and Biotechnology
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    • v.36 no.1
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    • pp.9-16
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    • 2021
  • Under the stressed condition, a complex feedback mechanism for stress is activated to maintain homeostasis of the body and secretes several stress hormones. But these stress hormones impair synthesis and secretion of the reproductive hormones, followed by suppression of ovarian function. Cytochrome P450 1A2 (CYP1A2) plays a major role in metabolizing exogenous substances and endogenous hormones, and its expression is recently identified at not only the liver but also several organs with respect to the pancreas, lung and ovary. Although the expression of CYP1A2 can be also affected by several factors, understanding for the changed pattern of the ovarian CYP1A2 expression upon stress induction is still limited. Therefore, CYP1A2 expression in the ovaries from immobilization stress-induced rats were assessed in the present study. The stress-induced rats in the present study exhibited the physiological changes in terms of increased stress hormone level and decreased body weight gains. Under immunohistological observation, the ovarian CYP1A2 expression in both control and the stressed ovary was localized in the antral to pre-ovulatory follicles. However, its expression level was significantly (p < 0.01) higher in the stress-induced group than control group. In addition, stress-induced group presented more abundant CYP1A2-positive follicles (%) than control group. Since expression of the ovarian CYP1A2 was highly related with follicle atresia, increased expression of CYP1A2 in the stressed ovary might be associated with changes of the ovarian follicular dynamics due to stress induction. We hope that these findings have important implications in the fields of the reproductive biology.

A Case Study on the Improvement of the Beauty of Photovoltaic Generator : Focusing on the case of installation on the vertical side wall of a building (태양광 발전기의 심미성 향상을 위한 사례분석 연구 : 건물 수직 측벽에 설치되는 사례를 중심으로)

  • Lee, Jae-Hyun;Park, Ji-Hoon;Nam, Won-Suk;Jang, Jung-Sik
    • Journal of the Korea Convergence Society
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    • v.11 no.12
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    • pp.97-103
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    • 2020
  • This study sets the solar power system installed and applied to the vertical side wall among the photovoltaic systems in the building as the scope of the research. The theoretical background was considered through literature research as a research method, and the current status, trends and characteristics of solar generator design installed and applied to domestic and foreign vertical side walls were then investigated and analyzed cases. As a result, the importance and necessity of photovoltaic generators, potential for power generation and growth were identified, and positive factors and directions were found for improving aestheticity. Based on this point, we would like to propose expected effects that can be applied to photovoltaic system design installed and applied to vertical side walls in the future, and confirm the direction and significance of the improvement of aesthetic quality of the proposal for development of thin film solar cell design technology using green facade design.

Immunohistological expression of cytochrome P450 1A2 (CYP1A2) in the ovarian follicles of prepubertal and pubertal rat

  • Hwang, Jong-Chan;Park, Byung-Joon;Kim, Hwan-Deuk;Baek, Su-Min;Lee, Seoung-Woo;Jeon, Ryoung-Hoon;Jang, Min;Bae, Seul-Gi;Yun, Sung-Ho;Park, Jin-Kyu;Kwon, Young-Sam;Kim, Seung-Joon;Lee, Won-Jae
    • Journal of Animal Reproduction and Biotechnology
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    • v.35 no.4
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    • pp.329-337
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    • 2020
  • Cytochrome P450 1A2 (CYP1A2) is a member of the cytochrome P450 superfamily enzymes in mammals and plays a major role in metabolizing endogenous hormones in the liver. In recent days, CYP1A2 expression has been found in not only the liver but also other tissues including the pancreas and lung. However, little information is available regarding the expression of CYP1A2 in the ovary, in spite of the facts that the ovarian follicle growth and atresia are tightly associated with controls of endocrine hormonal networks. Therefore, the expression of CYP1A2 in the ovaries of prepubertal and pubertal rats was investigated to assess its expression pattern and puberty-related alteration. It was demonstrated that the expression level of CYP1A2 was significantly (p < 0.01) higher in the pubertal ovaries than prepubertal counterparts. At the ovarian follicle level in both groups, whereas CYP1A2 expression was less detectable in the primordial, primary and secondary follicles, the strongly positive expression of CYP1A2 was localized in the granulosa cell layers in the antral and pre-ovulatory follicles. However, the ratio of CYP1A2-positive ovarian follicle was significantly (p < 0.01) higher in the ovary of pubertal group (73.1 ± 3.1%) than prepubertal one (41.0 ± 10.5%). During the Immunofluorescence, expression of CYP1A2 was mainly localized in Fas-positive follicles, indicating the atretic follicles. In conclusion, these results suggested that CYP1A2 expression was mainly localized at the atretic follicular cells and affected by the onset of puberty. Further study is still necessary but we hypothesize that CYP1A2 expresses in the atretic follicles to metabolize residue of the reproductive hormones. These findings may have important implications for the fields of reproductive biology of animals.

Influential Factors on Supercooling of Nineteen Fruits and Vegetables (과채류 19종의 과냉각 영향요인 분석)

  • Kim, Jinse;Park, Jong Woo;Jung, Hyun Kyung;Park, Seok Ho;Choi, Dong Soo;Kim, Yong Hoon;Lee, Soo Jang;Park, Chun Wan;Lee, Young Hee
    • Food Engineering Progress
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    • v.22 no.4
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    • pp.321-327
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    • 2018
  • The main purposes of this study were to identify the factors affecting the supercooling property and to improve the possibility of supercooling storage of fruits and vegetables. Freezing point and nucleation temperature, moisture content, hardness, sugar content, and pH of nineteen fruits and vegetables were measured and Pearson correlation analysis was performed. Freezing point showed a statistically significant correlation with moisture content and sugar content (p<0.01), while ice nucleation temperature showed a correlation (p<0.05) only for sugar content. In particular, the water content and sugar content did not show any correlation with the freezing supercooling difference (FSD). From the correlation analysis between FSD, aerobic bacteria, lactic acid bacteria, yeast, and mold, FSD showed a correlation (p<0.01) with aerobic bacteria. The experiments of the saline solutions inoculated with aerobic bacteria at different concentrations showed FSDs of about 2 for saline inoculated with 9.4 log CFU/mL and about 6 for saline inoculated lower than 5 log CFU/mL. Therefore, the aerobic bacteria concentration was determined to be a key factor affecting the supercooling storage of fruits and vegetables.

Improved Tree-Based ${\mu}TESLA$ Broadcast Authentication Protocol Based on XOR Chain for Data-Loss Tolerant and Gigh-Efficiency (데이터 손실에 강하고 효율적 연산을 지원하는 XOR 체인을 이용한 트리기반 ${\mu}TESLA$ 프로토콜 개선)

  • Yeo, Don-Gu;Jang, Jae-Hoon;Choi, Hyun-Woo;Youm, Heung-Youl
    • Journal of the Korea Institute of Information Security & Cryptology
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    • v.20 no.2
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    • pp.43-55
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    • 2010
  • ${\mu}TESLA$ broadcast authentication protocol have been developed by many researchers for providing authenticated broadcasting message between receiver and sender in sensor networks. Those cause authentication delay Tree-based ${\mu}TESLA$[3] solves the problem of authentication delay. But, it has new problems from Merkel hash tree certificate structure. Such as an increase in quantity of data transmission and computation according to the number of sender or parameter of ${\mu}TESLA$ chain. ${\mu}TPCT$-based ${\mu}TESLA$[4] has an advantages, such as a fixed computation cost by altered Low-level Merkel has tree to hash chain. However, it only use the sequential values of Hash chain to authenticate ${\mu}TESLA$ parameters. So, It can't ensure the success of authentication in lossy sensor network. This paper is to propose the improved method for Tree-based ${\mu}TESLA$ by using XOR-based chain. The proposed scheme provide advantages such as a fixed computation cost with ${\mu}$TPCT-based ${\mu}TESLA$ and a message loss-tolerant with Tree-based ${\mu}TESLA$.

Effect of Pallet Size Modified Atmosphere Packaging Using Polyamide Film and Linear Low Density Polyethylene Film on the Shelf Life of Strawberries (PA와 LLDPE 필름을 이용한 딸기 저장용 Modified Atmosphere Pallet Packaging 연구)

  • Kim, Jinse;Jung, Hyun Kyung;Nam, So Young;Son, Jae Yong;Park, Seok Ho;Choi, Dong Soo;Kim, Yong Hoon;Lee, Soo Jang;Park, Chun Wan
    • Food Engineering Progress
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    • v.23 no.1
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    • pp.55-61
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    • 2019
  • For the vessel export of strawberries, modified atmosphere package (MAP) using polyamide (PA) film and linear low density polyethylene (LLDPE) film was investigated to extend the shelf life of strawberries. Because the temperature and relative humidity changes of the MAP were lower than the changes of the control, the weight loss of the MAP were lower than that of the control. The low oxygen level and high carbon dioxide level were effective to decrease the fungal decay rate and to increase the hardness of strawberries. The Hunter's color differences before and after storage showed no distinct difference between the MAP and the control. The lightness had a tendency to decrease while the redness increased. There were no significant changes of the soluble solids during the storage. The shelf life of strawberries could be extended to 16 days using the MAP considering the weight loss and the fungal decay rate. Thus, this MAP method using PA film and LLDPE film was effective to extend the shelf life of strawberries.

A Study on the Development of 3D Virtual Reality Campus Tour System for the Adaptation of University Life to Freshmen in Non-face-to-face Situation - Autonomous Operation of Campus Surrounding Environment and University Information Guide Screen Design Using Visual Focus Movement - (비대면 상황에서 신입생 대학생활적응을 위한 3차원 가상현실 캠퍼스 투어시스템 개발연구 - 시야초점의 움직임을 활용한 캠퍼스주변 환경의 자유로운 이동과 대학정보안내화면 GUI설계 -)

  • Lim, Jang-Hoon
    • Journal of Information Technology Applications and Management
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    • v.28 no.3
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    • pp.59-75
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    • 2021
  • This study aims to establish a foundation for autonomous driving on campus and communication of abundant university information in the HCI environment in a VR environment where college freshmen can freely travel around campus facilities. The purpose of this study is to develop a three-dimensional VR-style campus tour system to establish a media environment to provide abundant university information guidance services to freshmen in non-face-to-face situations. This study designed a three-dimensional virtual reality campus tour system to solve the problem of discontinuity in which VR360 filming space does not lead to space like reality, and to solve many problems of expertise in VR technology by constructing an integrated production environment of tour system. We aim to solve the problem of inefficiency, which requires a large amount of momentum in virtual space, by constructing a GUI that utilizes the motion of the field of view focus. The campus environment was designed as a three-dimensional virtual reality using a three-dimensional graphic design. In non-face-to-face situations, college freshmen freely transformed the HMD VR device, smartphone, FPS operation mode of the gyroscope sensor. The design elements of the three-dimensional virtual reality campus tour system were classified as ①Visualization of factual experiences, ②Continuity of space movement, ③Operation, automatic operation mode, ④Natural landscape animation, ⑤Animation according to wind direction, ⑥Actual space movement mode, ⑦Informatization of spatial understanding, ⑧GUI by experience environment, ⑨Text GUI by building, ⑩VR360, 3D360 Studio Environment, ⑪Three-dimensional virtual space coupling block module, ⑫3D360-3D Virtual Space Transmedia Zone, ⑬Transformable GUI(VR Device Dual Viewer-Gyro Sensor Full Viewer-FPS Operation Viewer) and an integrated production environment was established with each element. It is launched online (http://vautu.com/u1) by constructing a GUI for free driving mode and college information screens to adapt to college life for freshmen, and designing an environment that can be used simultaneously by current media such as PCs, Android, and iPads. Therefore, it conducted user research, held a development presentation, a forum on excellence in university innovation support projects, and applied it as a system on the website of a particular university. College freshmen will be able to experience university information directly from the web and app to the virtual reality campus environment.

Improvement of Temperature Constancy of Direct Refrigerator for Supercooled Storage (직냉식 냉장고의 과냉각 저장을 위한 항온 특성 개선 연구)

  • Kim, Jinse;Nam, Soyoung;Jung, Hyun Kyung;Son, Jae Yong;Choi, Dong Soo;Kim, Yong Hoon;Lee, Soo Jang;Park, Chun Wan;Kim, Ha Yoon;Park, Seok Ho
    • Food Engineering Progress
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    • v.23 no.4
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    • pp.270-277
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    • 2019
  • Commercial direct refrigerators have good energy efficiency, but are difficult to use for supercooled storage due to their large temperature deviation. Placing insulators and conductors inside the refrigerator could reduce these temperature deviations to within 0.3 degrees, allowing for the supercooled storage. The supercooled storage of salted Chinese cabbages during ten weeks was progressed to compare the other low temperature storages. The nucleation temperatures of salted Chinese cabbage were around -2.5℃ and the freezing points were around -0.4℃, so -2℃ was selected for the supercooled storage. The growth rate of lactic acid bacteria and yeast at -2℃ storage was lower than that at 2℃ storage. The reducing sugar was maintained higher due to the growth rate of lactic acid bacteria. The supercooled storage had an effect of delaying the fermentation of the salted Chinese cabbage, which may have the effect of delaying the fermentation of kimchi. This enhancement method of the direct refrigerator was effective for the supercooled storage and would be promising for commercial use.

Effect of Jesaeng-sinkihwan on Renal Dysfunction in Ischemia/Reperfusion-Induced Acute Renal Failure Mouse (제생신기환이 허혈-재관류로 유발된 급성 신부전 마우스에 미치는 효과)

  • Han, Byung Hyuk;Lee, Hyeon Kyoung;Jang, Se Hoon;Tai, Ai Lin;Yoon, Jung Joo;Kim, Hye Yoom;Lee, Yun Jung;Lee, Ho Sub;Kang, Dae Gill
    • Herbal Formula Science
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    • v.29 no.1
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    • pp.33-44
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    • 2021
  • Renal ischemia-reperfusion injury(IRI), an important cause of acute renal failure (ARF), cause increased renal tubular injury. Jesaeng-sinkihwan (JSH) was recorded in a traditional Chines medical book named "Bangyakhappyeon (方藥合編)". JSH has been used for treatment of diabetes and glomerulonephritis with patients. Here we investigate the effects of Jesaeng-sinkihwan (JSH) in a mouse model of ischemic acute kidney injury. The animals model were divided into four groups at the age of 8 weeks; sham group: C57BL6 male mice (n=9), I/R group: C57BL6 male mice with I/R surgery (n=9), JSH Low group: C57BL6 male mice with surgery + JSH 100 mg/kg/day (n=9) and JSH High group: C57BL6 male mice with surgery + JSH 300 mg/kg/day (n=9). Ischemia was induced by clamping the both renal arteries during 25 min, and reperfusion was followed. Mouse were orally given with JSH (100 and 300 mg/kg/day during 3 days after surgery. Treatment with JSH significantly ameliorates creatinine clearance(Ccr), Creatinine (Cr) and blood urea nitrogen(BUN) in obtained plasma. . Treatment with JSH reduced kidney inflammation markers such as Neutrophil Gelatinase Associated Lipocalin (NGAL) and kidney injury molecule-1 (KIM-1). JSH also reduced the periodic acid schiff (PAS) staining intensity and picro sirius red staining intensity in kidney of I/R group. These findings suggest that JSH ameliorates tubular injury including renal dysfunction in I/R induced ARF mouse.

Comparison of Growth Characterisitics, Asiaticoside Content and Antioxidant Activities of Centella asiatica (L.) Urb. (병풀의 생육 특성, Asiaticoside 함량 및 항산화 활성 비교)

  • Choi, Jang Nam;Oh, Myeong Won;Lee, Hee Jung;Lee, Jeong Hoon;Jeong, Jin Tae;Lee, Yun Ji;Chang, Jae Ki;Park, Chun Geon
    • Korean Journal of Plant Resources
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    • v.34 no.1
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    • pp.44-51
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    • 2021
  • Centella asiatica (L.) Urban is a medicinal plant containing madecassic acid, triterpenoids and asiaticoside, and has a wound healing effect. However, since it grows only in some regions of Korea, it is necessary to breed stable varieties. In this study, it were analyzed for the growth characteristics, asiaticoside, anxiodant components and 2,2-diphenyl-1picryl hydrazyl (DPPH) radical scavening activity of C. asiatica collected from domestic natural habitat (Hapcheon, Gyeongnam). In the growth characteristics of each resource, CA9 and CA10 were superior in characteristics of aerial part and in dry weight. In asiaticoside content analysis, CA5 and CA3 were high with 53.96 mg/g and 52.40 mg/g, respectively. CA12 showed the possibility of development because the resource showed high in total flavonoid (7.3 mg/g), total polyphenol (10.64 mg/g) and DPPH activity (11.96 mg AAE/g) among other resources. However, it is necessary to select a resource suitable for each characteristic of C. asiatica and to study charateristics through additional resources collection since the excellent growth characteristics of the collected resources do not necessarily coincide with the content and antioxidant activities.