• Asian-Australasian Journal of Animal Sciences
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• 제22권6호
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• pp.871-884
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• 2009

Gene expression profiling is a useful tool for identifying critical genes and pathways in metabolism. The objective of this study was to determine the major differences in the expression of genes associated with metabolism and metabolic regulation in liver and mammary tissues of lactating cows. We used the Michigan State University bovine metabolism (BMET) microarray; previously, we have designed a bovine metabolism-focused microarray containing known genes of metabolic interest using publicly available genomic internet database resources. This is a high-density array of 70mer oligonucleotides representing 2,349 bovine genes. The expression of 922 genes was different at p<0.05, and 398 genes (17%) were differentially expressed by two-fold or more with 222 higher in liver and 176 higher in mammary tissue. Gene ontology categories with a high percentage of genes more highly expressed in liver than mammary tissues included carbohydrate metabolism (glycolysis, glucoenogenesis, propanoate metabolism, butanoate metabolism, electron carrier and donor activity), lipid metabolism (fatty acid oxidation, chylomicron/lipid transport, bile acid metabolism, cholesterol metabolism, steroid metabolism, ketone body formation), and amino acid/nitrogen metabolism (amino acid biosynthetic process, amino acid catabolic process, urea cycle, and glutathione metabolic process). Categories with more genes highly expressed in mammary than liver tissue included amino acid and sugar transporters and MAPK, Wnt, and JAK-STAT signaling pathways. Real-time PCR analysis showed consistent results with those of microarray analysis for all 12 genes tested. In conclusion, microarray analyses clearly identified differential gene expression profiles between hepatic and mammary tissues that are consistent with the differences in metabolism of these two tissues. This study enables understanding of the molecular basis of metabolic adaptation of the liver and mammary gland during lactation in bovine species.

• BMB Reports
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• 제44권10호
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• pp.686-691
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• 2011

Granulocyte colony-stimulating factor (G-CSF) is a cytokine secreted by stromal cells and plays a role in the differentiation of bone marrow stem cells and proliferation of neutrophils. Therefore, G-CSF is widely used to reduce the risk of serious infection in immunocompromised patients; however, its use in such patients is limited because of its non-persistent biological activity. We created an N-linked glycosylated form of this cytokine, hG-CSF (Phe140Asn), to assess its biological activity in the promyelocyte cell line HL60. Enhanced biological effects were identified by analyzing the JAK2/STAT3/survivin pathway in HL60 cells. In addition, mutant hG-CSF (Phe140Asn) was observed to have enhanced chemoattractant effects and improved differentiation efficiency in HL60 cells. These results suggest that the addition of N-linked glycosylation was successful in improving the biological activity of hG-CSF. Furthermore, the mutated product appears to be a feasible therapy for patients with neutropenia.

• Journal of Microbiology and Biotechnology
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• 제29권12호
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• pp.2006-2013
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• 2019

The isolation of respiratory viruses, especially from clinical specimens, often shows poor efficiency with classical cell culture methods. The lack of suitable methods to generate virus particles inhibits the development of diagnostic assays, treatments, and vaccines. We compared three inoculation methods, classical cell culture, the addition of a JAK2 inhibitor AZD1480, and centrifugation-enhanced inoculation (CEI), to replicate human respiratory syncytial virus (HRSV) and human metapneumovirus (HMPV). In addition, a combined method using AZD1480 treatment and CEI was used on throat swabs to verify that this method could increase virus isolation efficiency from human clinical specimens. Both CEI and AZD1480 treatment increased HRSV and HMPV genome replication. Also, the combined method using CEI and AZD1480 treatment enhanced virus proliferation synergistically. The combined method is particularly suited for the isolation of interferon-sensitive or slowly growing viruses from human clinical specimens.

• Environmental Analysis Health and Toxicology
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• 제22권2호통권57호
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• pp.129-135
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• 2007

Polycyclic aromatic hydrocarbons(PAHs) have been analyzed to assess vertical distribution of them with different land uses. The soils were collected from three layers; surface $(0{\sim}5cm)$, intermediate $(6{\sim}10cm)$, and deep $(11{\sim}15cm)$ layer, respectively considering land use; paddy, upland, and mountain in each site. Total 89 samples of soil from 10 sites were analyzed. Overall mean of ${\sum}PAHs$ were 137 (range $8.87{\sim}625{\mu}g\;kg^{-1}$), 203 (range $16.5{\sim}645{\mu}g\;kg^{-1}$), and $83.4{\mu}g\;kg^{-1}$ (range $6.65{\sim}667{\mu}g\;kg^{-1}$) for paddy, upland, and mountain soil, respectively. The dominant PAHs were fluoroanthene/benzo(b)fluoroanthene>pyrene>indeno(1, 2, 3-cd) pyrene in paddy, fluoroanthene/pyrene>benzo(b)fluoroanthene>chrysene in upland, and benzo(b)fluoroanthene>pyrene>chrysene in mountain soil, whereas the profile was quite similar for each other except that indeno(1, 2, 3-cd)pyrene and benzo(ghi)perylene are relatively higher in the paddy soils. Although the concentration gradient by depth was not observed in the paddy and upland soils because perturbation of soil layer by tillage, significant decrease was in the deep layer relative to the surface and intermediate layer. However, the concentration gradient of PAHs by soil depth was clearly shown in mountain soil without experiencing disturbance of tillage.

• The Korean Journal of Physiology and Pharmacology
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• 제13권3호
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• pp.161-168
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• 2009

In the peripheral nerves, injury-induced cytokines and growth factors perform critical functions in the activation of both the MEK/ERK and JAK/STAT3 pathways. In this study, we determined that nerve injury-induced ERK activation was temporally correlated with STAT3 phosphorylation at the serine 727 residue. In cultured Schwann cells, we noted that ERK activation is required for the serine phosphorylation of STAT3 by neuropoietic cytokine interleukin-6 (IL-6). Serine phosphorylated STAT3 by IL-6 was transported into Schwann cell nuclei, thereby indicating that ERK may regulate the transcriptional activity of STAT3 via the induction of serine phosphorylation of STAT3. Neuregulin-1 (NRG) also induced the serine phosphorylation of STAT3 in an ERK-dependent fashion. In contrast with the IL-6 response, serine phosphorylated STAT3 induced by NRG was not detected in the nucleus, thus indicating the non-nuclear function of serine phosphorylated STAT3 in response to NRG. Finally, we determined that the inhibition of ERK prevented injury-induced serine phosphorylation of STAT3 in an ex-vivo explants culture of the sciatic nerves. Collectively, the results of this study show that ERK may be an upstream kinase for the serine phosphorylation of STAT3 induced by multiple stimuli in Schwann cells after peripheral nerve injury.

• Asian Pacific Journal of Cancer Prevention
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• 제15권22호
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• pp.9791-9795
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• 2014

To study the gene expression change and possible signal pathway during androgen-dependent prostate cancer (ADPC) becoming androgen-independent prostate cancer (AIPC), an LNCaP cell model of AIPC was established using flutamide in combination with androgen-free environment inducement, and differential expression genes were screened by microarray. Then the biological process, molecular function and KEGG pathway of differential expression genes are analyzed by Molecule Annotation System (MAS). By comparison of 12,207 expression genes, 347 expression genes were acquired, of which 156 were up-ragulated and 191 down-regulated. After analyzing the biological process and molecule function of differential expression genes, these genes are found to play crucial roles in cell proliferation, differntiation, cell cycle control, protein metabolism and modification and other biological process, serve as signal molecules, enzymes, peptide hormones, cytokines, cytoskeletal proteins and adhesion molecules. The analysis of KEGG show that the relevant genes of AIPC transformation participate in glutathione metabolism, cell cycle, P53 signal pathway, cytochrome P450 metabolism, Hedgehog signal pathway, MAPK signal pathway, adipocytokines signal pathway, PPAR signal pathway, TGF-${\beta}$ signal pathway and JAK-STAT signal pathway. In conclusion, during the process of ADPC becoming AIPC, it is not only one specific gene or pathway, but multiple genes and pathways that change. The findings above lay the foundation for study of AIPC mechanism and development of AIPC targeting drugs.

• 식물분류학회지
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• 제42권4호
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• pp.330-334
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• 2012

골풀과의 골풀속에 속하는 Juncus fauriei L$\acute{e}$v. & Vaniot는 일본과 러시아에 분포한다고 알려져 있는 종인데, 강원도의 하조대 해안가 사구 습지에서 이를 발견하여 보고하고자 한다. 이 식물은 줄기의 단면이 납작하고 주로 나선상으로 꼬여있고, 화피편의 가장자리가 검은색을 띄는 특징으로 국내 골풀속 내의 section Juncotypus에 속하는 근연 종인, J. decipiens (골풀), J. setchuensis (푸른갯골풀), J. haenkei (갯골풀), J. brachyspathus (참골풀)와 확연하게 구분 된다. 이러한 형태적 특징에 따라 국명을 '검정납작골풀'로 신칭하였고, 이 분류군의 사진 및 형질에 대한 기재, 근연 분류군들과 구분할 수 있는 검색표를 제시하였다.

• 한국철도학회논문집
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• 제16권1호
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• pp.71-77
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• 2013

본 연구는 KTX승무서비스 종사자 263명을 대상으로 이들이 경험하는 감정노동을 내면행동과 표면행동으로 구분하고, 각각 감정소진과 이직의도에 미치는 영향을 실증 분석 하였다. 분석결과, KTX승무서비스 종사자의 내면행동은 감정소진에 유의미한 영향을 미치지 않았으나, 표면행동은 정(+)의 영향을 미치는 것으로 나타났다. 또한 KTX 승무서비스 종사자의 내면행동은 이직의도에 부(-)의 영향을 미쳤으나, 표면행동은 유의미한 영향을 미치지 않는 것으로 나타났다. 마지막으로 KTX승무서비스 종사자의 감정소진은 이직의도에 유의미한 정의 영향을 미치는 것으로 나타났다. 결론적으로 본 연구는 KTX승무서비스 종사자의 이직예방을 위해서는 감정관리가 중요하다는 사실을 시사하고 있다.

• 한국토양비료학회지
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• 제31권3호
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• pp.253-258
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• 1998

마이크로파 시료 분해법이 식물체와 퇴비 중의 K, Ca, Mg, Cu, Zn, Mn등을 분석하는데 적합한가를 검정하기 위하여 기존의 습식분해법과 비교하였다. 분석시간은 기존의 습식분해법이 4~6시간 걸리던 것에 비하여 마이크로파 분해법에서는 1시간 이하로 단축할 수 있었으며, 두 분석법간의 각 성분별 분석값의 상관계수($R^2$)는 각각 K 0.98, Ca 0.97, Mg 0.91, Zn 0.94, Mn 0.99, Cu 0.99를 나타내었다. 칼륨과 칼슘의 경우 기존 습식분해법의 분석값이 더 높은 경향을 나타내었고 구리, 망간, 아연의 경우는 마이크로파 분해법의 분석값이 더 높은 경향을 나타내었으나 마그네슘의 경우 일정한 경향이 없었다. 그러나 칼슘의 경우를 제외한 그 외 성분들의 편기도(biases)는 10% 이내였다.

• Animal Systematics, Evolution and Diversity
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• 제17권2호
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• pp.171-177
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• 2001

작약도에서 서식하고 있는 쌍뾰족혹히드라(Obelia bicuspidata Clarke, 1875)의 생활사를 서식지와 실험실에서 조사하였다. 쌍뾰족혹히드라는 종히드라과(Campanulariidae)에 속하고 주로 뻘 해안에 있는 작은 바위 또는 굴의 패각에 부착한다. 해파리를 방출하며 해파리 눈(medusa buds)은 일년에 2번, 즉 3월과 6월 사이, 9월과 10월 사이에 발달한다. 폴립 성장의 최적온도는 4-8$^{\circ}C$로 나타났고 수온이 9$^{\circ}C$이상으로 올라가면 퇴화하기 시작하여 수표면 온도가 약 25一되는 8월에는 히드라협이 모두 퇴화하는 것으로 나타났다. 해파리는 작고 폴립에서 방출된 후 약 9일 후 생식선이 발달하였다. 본 연구에서 쌍뾰족혹히드라는 냉수성 종으로 판명되었다.