• BMB Reports
• /
• 제50권5호
• /
• pp.269-274
• /
• 2017

The biological activities of macrophage migration inhibitory factor (MIF) might be mediated through a classical receptor-mediated or non-classical endocytic pathway. JAB1 (C-Jun activation domain-binding protein-1) promotes the degradation of the tumor suppressor, p53, and the cyclin-dependent kinase inhibitor, p27. When MIF and JAB1 are bound to each other in various intracellular sites, MIF inhibits the positive regulatory effects of JAB1 on the activity of AP-1. The intestinal parasite, Anisakis simplex, has an immunomodulatory effect. The molecular mechanism of action of As-MIF and human JAB1 are poorly understood. In this study, As-MIF and hJAB1 were expressed and purified with high solubility. The structure of As-MIF and hJAB1 interaction was modeled by homology modeling based on the structure of Ace-MIF. This study provides evidence indicating that the MIF domain of As-MIF interacts directly with the MPN domain of hJAB1, and four structure-based mutants of As-MIF and hJAB1 disrupt the As-MIF-hJAB1 interaction.

• BMB Reports
• /
• 제42권5호
• /
• pp.299-303
• /
• 2009

In this study, we use promoter analysis to show that interaction between Jab1 and p53 induces suppression of p53 activation in U2OS and H1299 cells. Interaction between p53 and Jab1 was further confirmed by immunoprecipitation and immunofluorescent analyses. In particular, Jab1 was able to induce nuclear export of p53 as previously reported. When Jab1 was overexpressed in U2OS cells followed by etoposide or hydrogen peroxide ($H_2O_2$), cell death induced by such stresses was protected against. On the contrary, when the level of Jab1 was suppressed in U2OS cells, cytotoxicity imposed by etoposide and $H_2O_2$ was dramatically increased, suggesting a cell protective role for Jab1. These results indicate that Jab1 is a negative regulator of p53 and a plausible oncogene.

• BMB Reports
• /
• 제50권7호
• /
• pp.373-378
• /
• 2017

The Jun activation-domain binding protein 1 (Jab1) induces p53 nuclear export and cytoplasmic degradation, but the underlying mechanism is poorly understood. Here, we show that phosphorylation at the threonine 155 residue is essential for Jab1-mediated p53 nuclear export. Jab1 stimulated phosphorylation of p53 at T155 was inhibited by curcumin, an inhibitor of COP9 signalosome (CSN)-associated kinases. The T155E mutant, which mimics phosphorylated p53, exhibited spontaneous cytoplasmic localization in the absence of Jab1. This process was prevented by leptinomycin B (LMB), but not by curcumin. The substitution of threonine 155 for valine (T155V) abrogated Jab1-mediated p53 nuclear export, indicating that phosphorylation at this site is essential for Jab1-mediated regulation of p53. Although T155E can be localized in the cytoplasm in the absence of Mdm2, the translocation of T155E was significantly enhanced by ectopic Hdm2 expression. Our data suggests that Jab1-mediated phosphorylation of p53 at Thr155 residue mediates nuclear export of p53.

• Molecules and Cells
• /
• 제22권2호
• /
• pp.133-140
• /
• 2006

Jun activation domain-binding protein 1 (Jab1) is involved in various cellular mechanisms including development in Drosophila and mouse, cell cycle control and signal transduction pathways. Recent studies also determined that Jab1 functions as a nuclear exporter and inducer of cytoplasmic degradation for several proteins including p53, p27, capsid of West Nile virus, and Smad4/7 proteins. In particular, p53 is shown to bind to and to be exported into the cytoplasm by Jab1, which helps to maintain low levels of p53 under normal conditions. This review was undertaken in an effort to understand the biological significance of the homeostasis of p53 as maintained in the presence of Jab1. Based on our observations, we have provided potential mechanistic hypotheses for the nuclear export of p53 in coordination with Jab1 and the role of other factors in these processes.

• Asian Pacific Journal of Cancer Prevention
• /
• 제13권5호
• /
• pp.2115-2120
• /
• 2012

The presence of lung cancer cells in anoxic zones is a key cause od chemotherapeutic resistance. Thus, it is necessary to enhance the sensitivity of such lung cancer cells. However, loss of efficient gene therapeutic targeting and inefficient objective gene expression in the anoxic zone in lung cancer are dilemmas. In the present study, a eukaryotic expression plasmid pUC57-HRE-JAB1 driven by a hypoxia response elements promoter was constructed and introduced into lung cancer cell line A549. The cells were then exposed to a chemotherapeutic drug cis-diamminedichloroplatinum (C-DDP). qRT-PCR and western blotting were used to determine the mRNA and protein level and flow cytometry to examine the cell cycle and apoptosis of A549 transfected pUC57-HRE-JAB1. The results showed that JAB1 gene in the A549 was overexpressed after the transfection, cell proliferation being arrested in G1 phase and the apoptosis ratio significantly increased. Importantly, introduction of pUC57-HRE-JAB1 significantly increased the chemotherapeutic sensitivity of A549 in an anoxic environment. In conclusion, JAB1 overexpression might provide a novel strategy to overcome chemotherapeutic resistance in lung cancer.

• 한국응용과학기술학회지
• /
• 제37권2호
• /
• pp.232-240
• /
• 2020

본 연구는 복싱 잽 동작 시 제자리 스텝의 사용이 운동역학적 요인들에 미치는 영향을 분석 하는데 목적이 있었다. 이를 위해 최근 1년간 근골격계에 상해가 없었던 오른손잡이 스탠스 유형의 대학교 복싱 선수 8명(나이: 20.38±0.52 yrs, 신장: 172.38±5.80 cm 체중: 63.45±8.56 kg, 경력 6.00±1.07 yrs)이 실험에 참여하였다. 잽 동작 시 제자리 스텝 사용이 운동역학적 요인들에 미치는 영향을 검증하기 위하여 대응표본 t-test(α = .05) 통계방법을 사용하였으며 다음과 같은 결과를 도출하였다. 첫째, W.S(with-step) 가 N.S(non-step) 보다 더 큰 충격력을 나타내었고, 근육의 활성도는 낮은 것으로 분석되었다. 둘째 W.S가 인체 분절의 전방의 속도의 영향을 미쳐서 골반과 발 분절이 더 빠르게 이동하는 것으로 분석되었다. 셋째, W.S는 골반의 회전 움직임이 더 빠른 것으로 나타났다. 넷째, W.S는 N.S보다 상대적으로 오른발과 왼발에 의해서 발생된 전방의 지면반발력이 더 큰 것으로 분석되었다. 이를 통해서 복싱 잽 동작 시 제자리 스텝의 사용은 지면반발력을 증가시켜 인체 분절의 이동 속도와 회전 움직임에 영향을 미치는 것으로 나타났다. 따라서 더 빠르고 민첩한 움직임을 가능케 하여 상대적으로 적은 근육의 사용으로도 더 큰 충격력을 내는 것으로 확인되었다. 그러므로 복싱의 잽 동작 시 효율적으로 상대방에게 큰 충격력을 전달하기 위해서는 제자리 스텝을 동반하는 것이 효과적으로 분석된다.

• Management Science and Financial Engineering
• /
• 제9권1호
• /
• pp.1-10
• /
• 2003

We introduce a new estimator of the uncertainty of a jackknife estimate of standard error: the jack-knife-after-jackknife (JAJ). Using Monte Carlo simulation, we assess the accuracy of the JAJ in a variety of settings defined by statistic of interest, data distribution, and sample size. For comparison, we also assess the accuracy of the jackknife-after-bootstrap (JAB) estimate of the uncertainty of a bootstrap standard error. We conclude that the JAJ provides a useful new supplement to Tukey's jackknife, and the combination of jackknife and JAJ provides a useful alternative to the combination of bootstrap and JAB.

• Management Science and Financial Engineering
• /
• 제9권1호
• /
• pp.4-10
• /
• 2003

We introduce a new estimator of the uncertainty of a jackknife estimate of standard error: the jack-knife-after-jackknife (JAJ). Using Monte Carlo simulation, we assess the accuracy of the JAJ in a variety of settings defined by statistic of interest, data distribution, and sample size. For comparison, we also assess the accuracy of the jackknife-after-bootstrap (JAB) estimate of the uncertainty of a bootstrap standard error. We conclude that the JAJ provides a useful new supplement to Tukey's jackknife, and the combination of jackknife and JAJ provides a useful alternative to the combination of bootstrap and JAB.

• 한국의사학회지
• /
• 제24권1호
• /
• pp.57-62
• /
• 2011

With the discovery of WooJam JabJeo, Jang-Taegyung[1809~1887] was highlighted as a noted doctor who won fame in the neighborhood of Gwangju, but with 'WoodJam Mango' seeing the light of day, this study was able to confirm the appearance of a literary man enjoying a reputation enough to sway literary circles with his writing of poems. It's possible to confirm his past related to medical service only until age of 42, which is because description in "WooJam JabJeo" came to a halt there. The situation is that the contents of 'WooJam Mango' started from age of 46 and were carried on even until after his death by his followers. Therefore, there exists a very intense impression that he might have put a focus on the life as a literary man ever after his mid-40s. and considering that he breathed his last at the age of 79, it's not difficult for us to estimate that his life was predominantly a series of literary activities rather than a health care provider. The place names appearing in "WooJam JabJeo" and "WooJam Mango" are limited to today's Jeonlanam-do, and this researcher would say that the through-composed poems, which sang of the beauty of Gwangju area scenery are the works, deserve to get a spotlight in local history even today. Through the above discussion, this study could prove that WooJam is a flawless figure enough to represent Gwangju and Jeonlanam-do as a health care provider and a literary person as well.

• 한국융합학회논문지
• /
• 제9권11호
• /
• pp.145-152
• /
• 2018

본 연구의 목적은 근로자의 건강행위실천 및 직업적 특성과 치주질환 간의 관련성 분석을 통해 근로자의 구강건강 증진사업에 필요한 기초자료를 제공하는 것이다. 2015년도 국민건강영양조사를 이용하여 만 19세 이상 총 1,737명의 근로자를 연구대상으로 하였으며, 자료는 SPSS 24.0 프로그램을 이용하여 분석하였다. 근로자의 건강행위실천에 따른 치주질환 위험도는 흡연, 비만, 유산소 신체활동 실천에서 통계적으로 유의한 차이를 보였으며, 직업특성에 따른 치주질환 위험도에서는 직업의 종류, 근무상태, 정규직여부에서 통계적으로 유의한 차이를 보였다. 이상의 결과에서 근로자의 치주질환은 건강행위실천과 사회경제적 수준에 영향을 받기에 근로자의 건강행위실천을 위한 사업주들의 적극적인 관리와 저소득층을 위한 구강보건의료정책 개발을 통한 의료서비스 양극화 해소가 필요할 것으로 사료된다.