• Journal of Life Science
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• v.17 no.12
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• pp.1627-1633
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• 2007

c-Jun N-terminal kinase (JNK)-interacting protein 1 (JIP1), also known as Islet-brain 1 (IB1), is a scaffold protein that is highly expressed in neurons and pancreatic ${\beta}-cells$. In this study subcellular localization of JIP was investigated in cultured rat hippocampal neurons using an antibody that recognize all variants of JIP1, JIP-2 and JIP-3. The overall expression profile of JIP is punctate throughout soma and dendrites. Statistic analysis showed that $54.8{\pm}4.0%\;and\;94.1{\pm}4.5%$ of total JIP immunopuncta overlapped with those of excitatory postsynaptic markers SD-95 and ${\alpha}Camik$, respectively. In contrast, only $8.6{\pm}0.5%\;and\;7.3{\pm}0.5%$ of JIP clusters overlapped with those of inhibitory postsynaptic markers glycine receptor (GlyR) and gephyrin, respectively. JIP clusters overlapped or juxtaposed with SV2 but not GAD, markers for general and inhibitory nerve terminals, respectively. A substantial fraction $(29.3{\pm}1.0%)$ of flotillin immunopuncta, a marker for lipid rafts, clusters overlapped with those of JIP. In addition, JIP was highly expressed in some select ends of dendrites but minimal in axons. These data suggest important roles of JIP in excitatory postsynaptic sites, lipid rafts and dendritic ends.

• Journal of the Korean housing association
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• v.19 no.5
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• pp.121-129
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• 2008

This research considers the characteristics of the plan of the vernacular form of dwelling in KoCheon-Ri village, situated in the Yong-Dong mountain region. As part of this study, an analysis of local diversity is carried out. The current existing village houses in Kocheon-Ri can be categorized according to their floor structure, either as a wooden-floored or an Ondol-floored Kyup-jip(a double row house type). It was found that the wooden-flooded Kyup-jip house has been transformed into Ondol-floorded Kyup-jip due to a range of various factors. In order to clarify the characteristics of the vernacular dwelling type occurring in a particular area, it is important to determine the typical form that appears within the broader area. However, differences is also essential.

• Journal of Educational Research in Mathematics
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• v.13 no.4
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• pp.429-446
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• 2003

Gu-il-jip is a mathematical book of Chosun dynasty in the 18c. It consists of nine chapters including more than 473 problems and their solutions. Analyzing the problems and their solutions, we can appreciate the mathematical researches by the professional mathematicians of Chosun. Especially, it is worth noting the followings: - units for measuring and decimal notations - $\pi$, area of circle, volume of sphere - naming the powers - counting rods - excess and deficit: calculation technique for excess-deficit relations among quantities - rectangular arrays: calculation technique for simultaneous linear equations - 'Thien Yuan' notation: method for representing equations - 'Khai Fang': algorithm for numerical solution of quadratic, cubic and higher equations Based on these analyses, some pedagogical applications are proposed.

• Journal of Microbiology
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• v.41 no.1
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• pp.46-51
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• 2003

RAD6 in yeast mediates postreplication DNA repair and is responsible for DNA-damage induced mutations. RAD6 encodes ubiquitin-conjugating enzyme that is well conserved among eukaryotic organisms. However, the molecular targets and consequences of their ubiquitination by Rad6 have remained elusive. In Aspergillus nidulans, a RAD6 homolog has been isolated and shown to be an allele of uvs). We screened a CDNA library to isolate UVSJ-interacting proteins by the yeast two-hybrid system. JIPA was identified as an interactor of UVSJ. Their interaction was confirmed in vitro by a GST-pull down assay. JIPA was also able to interact with mutant UVSJ proteins, UVSJl and the active site cysteine mutant UVSJ-C88A. The N- and the C-terminal regions of UVSJ required for the interaction with UVSH, a RAD18 homolog of yeast which physically interacts with Rad6, were not necessary for the JIPA and UVSJ interactions. About 1.4 kb jipA transcript was detected in Northern analysis and its amount was not significantly increased in response to DNA-damaging agents. A genomic DNA clone of the jipA gene was isolated from a chromosome I specific genomic library by PCR-sib selection. Sequence determination of genomic and cDNA of jipA revealed an ORF of 893 bp interrupted by 2 introns, encoding a putative polypeptide of 262 amino acids. JIPA has 33% amino acid sequence identity to TIP41 of Saccharomyces cerevisiae which negatively regulates the TOR signaling pathway.

• Korean Journal of Medicinal Crop Science
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• v.22 no.1
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• pp.38-45
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• 2014

This study was performed to investigate the physiological responses of Oplopanax elatus by water condition. Drought stress was induced by withholding water for 26 days. The results show that $P_{N\;max}$, SPAD, gs, E and Ci were significantly decreased with decreasing of soil moisture contents. However, AQY and WUE were decreased slightly only at 26 day. This implies that photosynthetic rate is reduced due to an inability to regulate water and $CO_2$ exchange through the stomatal. According to JIP analysis, ${\Phi}_{PO}$, ${\Psi}_O$, ${\Phi}_{EO}$ and $PI_{ABS}$ were dramatically decreased at 21 day and 26 day, which reflects the relative reduction state of the photosystem II. On the other hand, the relative activities per reaction center such as ABS/RC, TRo/RC were significantly increased at 26 day. Particularly, Dio/RC and DIo/CS increased substantially under drought stress, indicating that excessive energy was consumed by heat dissipation. These results of chlorophyll a fluorescence show that the sensitivity changes photosystem II activity. Thus, according to the results, O. elatus was exhibited a strong reduction of photosynthetic activity to approximately 10% soil moisture contents, and JIP parameters could be useful indicator to monitor the physiological states of O. elatus under drought stress.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.32 no.2
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• pp.74-81
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• 2004

• Proceedings of the Korean Ceranic Society Conference
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• 2003.04a
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• pp.101.1-101
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• 2003

• Proceedings of the Korean Ceranic Society Conference
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• 2003.10a
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• pp.114.1-114
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• 2003

• Proceedings of the Korean Ceranic Society Conference
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• 2003.10a
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• pp.39.1-39
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• 2003

• 한국생물공학회:학술대회논문집
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• 2005.04a
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• pp.429-429
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• 2005