• Journal of Microbiology and Biotechnology
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• 제20권1호
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• pp.21-29
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• 2010

The phenolic compounds are a major contaminant class often found in industrial wastewaters and the biological treatment is an alternative tool commonly employed for their removal. In this sense, monitoring microbial community dynamics is crucial for a successful wastewater treatment. This work aimed to monitor the structure and activity of the bacterial community during the operation of a laboratory-scale continuous submerged membrane bioreactor (SMBR), using PCR and RT-PCR followed by denaturing gradient gel electrophoresis (DGGE) and 16S rRNA libraries. Multivariate analyses carried out using DGGE profiles showed significant changes in the total and metabolically active dominant community members during the 4-week treatment period, explained mainly by phenol and ammonium input. Gene libraries were assembled using 16S rDNA and 16S rRNA PCR products from the fourth week of treatment. Sequencing and phylogenetic analyses of clones from the 16S rDNA library revealed a high diversity of taxa for the total bacterial community, with predominance of Thauera genus (ca. 50%). On the other hand, a lower diversity was found for metabolically active bacteria, which were mostly represented by members of Betaproteobacteria (Thauera and Comamonas), suggesting that these groups have a relevant role in the phenol degradation during the final phase of the SMBR operation.

• Journal of Microbiology and Biotechnology
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• 제20권1호
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• pp.110-116
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• 2010

Lactic acid (LA) fermentation by Lactobacillus salivarius ATCC 11741 immobilized on loofa sponge (LS) was evaluated. To increase the surface area of LS for cell immobilization, $H_2O_2$ and chitosan were introduced as surface modifying reagents. Four chitosans of different molecular weights were separately coated on LS. All experiments were conducted in shaking flask mode at 100 rpm rotating speed and $37^{\circ}C$ with 5% $CaCO_3$ as a pH regulating agent. The effects of initial glucose concentration were investigated in the range of 20-100 g/l on LA fermentation by free cells. The results indicate that the maximum concentration of LA was produced with 50 g/l glucose concentration. The immobilized cell system produced 1.5 times higher concentration than free cells for 24 h of fermentation. Moreover, immobilized cells can shorten the fermentation time by 2-fold compared with free cells at the same level of LA concentration. At 1% (w/v) chitosan in 2% (v/v) acetic acid, the Yp/s and productivities of various molecular weights of chitosans were insignificantly different. Repeated batch fermentations showed 5 effective recycles with Yp/s and productivity in the range of 0.55-0.85 and 0.90-1.20 g/l.h, respectively. It is evident that immobilization of L. salivarius onto LS permits reuse of the system under these fermentation conditions. Scanning electron micrographs indicated that there were more intact cells on the chitosan-treated LS than on the untreated LS, thus confirming the effectiveness of the LS-chitosan combination when being utilized as a promising immobilization carrier for LA fermentation.

• Journal of Microbiology and Biotechnology
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• 제20권1호
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• pp.169-178
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• 2010

The microbial community structure in Thailand soils contaminated with low and high levels of arsenic was determined by denaturing gradient gel electrophoresis. Band pattern analysis indicated that the bacterial community was not significantly different in the two soils. Phylogenetic analysis obtained by excising and sequencing six bands indicated that the soils were dominated by Arthrobacter koreensis and $\beta$-Proteobacteria. Two hundred and sixty-two bacterial isolates were obtained from arsenic-contaminated soils. The majority of the As-resistant isolates were Gramnegative bacteria. MIC studies indicated that all of the tested bacteria had greater resistance to arsenate than arsenite. Some strains were capable of growing in medium containing up to 1,500 mg/l arsenite and arsenate. Correlations analysis of resistance patterns of arsenite resistance indicated that the isolated bacteria could be categorized into 13 groups, with a maximum similarity value of 100%. All strains were also evaluated for resistance to eight antibiotics. The antibiotic resistance patterns divided the strains into 100 unique groups, indicating that the strains were very diverse. Isolates from each antibiotic resistance group were characterized in more detail by using the repetitive extragenic palindromic-PCR (rep-PCR) DNA fingerprinting technique with ERIC primers. The PCR products were analyzed by agarose gel electrophoresis. The genetic relatedness of 100 bacterial fingerprints, determined by using the Pearson product-moment similarity coefficient, showed that the isolates could be divided into four clusters, with similarity values ranging from 5-99%. Although many isolates were genetically diverse, others were clonal in nature. Additionally, the arsenic-resistant isolates were examined for the presence of arsenic resistance (ars) genes by using PCR, and 30% of the isolates were found to carry an arsenate reductase encoded by the arsC gene.

• 한국해양환경ㆍ에너지학회지
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• 제13권2호
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• pp.75-82
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• 2010

FRP 선박은 제작이 쉽고 저렴한 건조비용으로 소형 선박을 중심으로 국내에 보급되었지만, 수명이 다한 노후 FRP 선박이 하천이나 어항 내에 방치되어 운항안전과 해양환경보전에 위협을 주는 등 사회문제로 대두대고 있다. 이를 개선하기 위하여 폐 FRP 선박 처리 기술과 용융처리시스템이 개발되었으며, 현지 적용을 위해 폐 FRP선박 처리 단계에 따른 전처리 지역 및 용융처리시스템 입지 방안이 필요하게 되었다. 폐 FRP선박 용융처리시스템 입지 장소는 기술적, 경제적, 환경적으로 적정해야 하므로 종합적인 차원의 평가가 요구되어 적지 선정에 어려움이 있다. 본 연구에서는 폐 FRP 용융처리시스템의 최적 입지 선정을 위해 입지경제적 요인, 생활환경 요인, 기반인프라 요인의 상위 요인과 각 상위 요인의 세부요인을 도출하여 폐 FRP선박 관련자를 대상으로 설문을 수행하였다 또한, Fuzzy AHP, 극한확률 이론을 이용하여 평가요인 간의 중요 가중치, 연관 가중치를 계산하고 후보지로 선정한 목포시, 강진군, 고흥군, 여수시에 대한 평가 결과를 검토 하였다.

• 로봇학회논문지
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• 제7권4호
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• pp.259-266
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• 2012

In these days, researches about underwater robots have been actively in progress for the purposes of ocean detection and resource exploration. Unlike general underwater robots such as ROV(Remotely Operated Vehicle) and AUV(Autonomous Underwater Vehicle) which have propellers, an articulated underwater robot which is called Crabster has been being developed in KORDI(Korea Ocean Research & Development Institute) with many cooperation organizations since 2010. The robot is expected to be able to walk and swim under the sea with its legs. Among many researching fields of this project, we are focusing on a swimming section. In order to find effective swimming locomotion for the robot, we approached this subject in terms of Biomimetics. As a model of optimized swimming organism in nature, diving beetles were chosen. In the paper, swimming motions of diving beetles were analyzed in viewpoint of robotics for applying them into the swimming motion of the robot. After modeling the kinematics of diving beetle through robotics engineering technique, we obtained swimming patterns of the one of living diving beetles, and then compared them with calculated optimal swimming patterns of a robot leg. As the first trial to compare the locomotion data of legs of the diving beetle with a robot leg, we have sorted two representative swimming patterns such as forwarding and turning. Experimental environment has been set up to get the motion data of diving beetles. The experimental equipment consists of a transparent aquarium and a high speed camera. Various swimming motions of diving beetles were recorded with the camera. After classifying swimming patterns of the diving beetle, we can get angular data of each joint on hind legs by image processing software, Image J. The data were applied to an optimized algorithm for swimming of a robot leg which was designed by robotics engineering technique. Through this procedure, simulated results which show trajectories of a robot leg were compared with trajectories of a leg of a diving beetle in desired directions. As a result, we confirmed considerable similarity in the result of trajectory and joint angles comparison.

• Journal of Microbiology and Biotechnology
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• 제20권7호
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• pp.1134-1139
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• 2010

This study explored the chronic effects of different doses of the triazole fungicide tebuconazole on the growth, and metabolic and enzymatic functions of the filamentous paddy field cyanobacterium, Westiellopsis prolifica Janet. The growth of the cyanobacterium was determined by an estimation of the change in pigment contents. Chlorophyll-a, carotenoids, and accessory pigments such as phycocyanin, allophycocyanin, and phycoerythrin were shown to decline over a 16-day period by a factor of 92%, 93%, 83%, 95%, and 100%, respectively, with increasing doses of the fungicide. Metabolic and enzymatic activities were also adversely affected. Over the 16 days, a gradual rise in total phenol content was recorded when Westiellopsis prolifica Janet was treated with 60 ppm of the fungicide, despite the reduction in carbohydrates, proteins, and amino acids by 96%, 92%, and 90%, respectively. Moreover, the enzymes nitrate reductase (NR), glutamine synthetase (GS), and succinate dehydrogenase (SDH) also registered reductions of 93%, 90%, and 98%, respectively. This study indicates that tebuconazole, although an important fungicide used extensively in rice fields, exhibits an inhibitory effect on the growth and metabolic activities of Westiellopsis prolifica Janet and hence possibly on other varieties as well.

• 대한의용생체공학회:의공학회지
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• 제31권3호
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• pp.227-233
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• 2010

This study analyzed performance according to kick point and stiffness of Soft-$golf^{TM}$ shaft. This research team developed soft-$golf^{TM}$ as a new fusion sports with similar motions with golf and it can be learned safely for all age groups in 2002. The head of Soft-$golf^{TM}$ club is made of zinc alloy and has a mesh or a grid structure, and shaft uses carbon graphite to reduce the total weight of the club. To improve carry distance and to assure consistency of a ball during Soft-$golf^{TM}$ swing, this study manufactured shaft with various kick points (low, middle and high) and stiffness (stiff, regular, lady, morelady) and analyzed a swing motion with characteristics of each shaft presented in a dynamic condition such as a ball's speed, a head's torsion angle and a ball's deviation with ProAnalyst program through a high-speed camera taking pictures using a swing machine robot system(Robo-7). From all of the results, this study determined an appropriate shaft of Soft-$golf^{TM}$.

• 한국안전학회지
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• 제25권4호
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• pp.13-18
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• 2010

To investigate electrostatic ignition hazards of commercial gasoline used in the gas station, experiments were conducted dealing with the minimum ignition energy(MIE) of several kinds of gasoline under the various temperature. The conductivity of gasoline that was required for an accurate risk assessment as well as the MIE were also examined. The solvent ignitability apparatus which can heat up the inside of the vessels up to $210^{\circ}C$ was used in this study. Four kinds of premium gasoline and four kinds of regular gasoline, differing with respect to the companies, were used as test specimens. The following results were obtained: (1) all gasoline specimens were so sensitive that even an electrostatic discharge with a very low energy, such as about 0.5mJ, could ignite them. The ignitability of premium gasoline was constant irrespective of the companies. On the other hand, the ignitability of regular gasoline was variable depending on the company. (2) The MIE of all specimens depended markedly on the temperature; in other words, an increase in temperature decreases the ignition energy value. (3) The conductivity values of all specimens were low. Those must be taken into consideration in electrostatic risk assessment.

• KIEAE Journal
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• 제10권3호
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• pp.57-62
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• 2010

To effectively use Building Information Modelling (BIM) dealing with semantic information including the entities of building components, the information about building components should be standardized. Like standardized modern buildings. in the past, Korean traditional houses were built according to strict procedures and formats. Therefore, if the Korean traditional house are modelled by using BIM,. not only the Korean traditional house of good quality will be built quickly and cheaply, but also spaces in the existing Korean traditional house will be easily analyzed. However, when analyzing spaces of the Korean traditional house using Space Syntax, some problems are caused in dividing outdoor space such as yard with unclear boundaries, unlike indoor space with clear boundaries surrounded by walls. These comes from the fact that researchers have subjectively divided a space in the house into convex spaces as units for Space Syntax analysis. Therefore, this study aims to develop an objective and rational spatial subdivision technique for Space Syntax analysis of a Korean traditional house modelled by using BIM. We could objectively and reasonably divide a Korean traditional house space into convex spaces by recognizing the building components in the house modelled in the form of Industry Foundation Classes(IFC). Depending on the connection of convex spaces allocated in the spatial subdivision technique, j-graph in Space Syntax could be drawn and the measurements of spatial configurations could be determinded. Through the developed technique, the social properties including the cultural and philosophical aspects of Korean people was identified by measuring the spatial configurations of Korean traditional house. The developed technique will serve as useful means to help architects to find an appropriate purpose of each space for sustainable architecture on the basis of the spatial and social relationships in buildings or urban systems.

• 한국정보과학회논문지:컴퓨팅의 실제 및 레터
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• 제16권2호
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• pp.237-241
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• 2010

최근 서버시스템 에너지 절감이 중요하게 대두되고 있다. 서버시스템에서 스토리지는 에너지 사용량이 매우 큰 서브시스템이지만, RAID와 같은 스트라이핑 기법과 하드디스크의 물리적 특성 때문에 실용적인 에너지 절감기법 개발에 어려움이 따른다. 본 연구팀의 관찰에 따르면 데이터 센터에서 전체 데이터 셋의 크기에 비해 하루 또는 몇 시간과 같이 짧은 기간의 풋프린트는 매우 작다. 본 논문은 이러한 관찰에 기반하여 SSD를 그러한 풋프린트를 감당하기 위한 커다란 캐시로 활용하여 에너지를 절약하는 새로운 아키텍처와 기법을 제안한다. 제안된 기법을 Linux 환경에서 네 개의 하드디스크와 한 개의 SSD로 구성된 RAID5에 구현하고 전력 사용량 측정 장치를 이용해 실제 전력 소모량을 측정한 결과 널리 사용되는 서버 트레이스에서 최대 14%의 저장장치 에너지 절감 효과를 얻었다.