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Effects of Dietary Bacillus-based Probiotic on Growth Performance, Nutrients Digestibility, Blood Characteristics and Fecal Noxious Gas Content in Finishing Pigs

  • Chen, Y.J.;Min, B.J.;Cho, J.H.;Kwon, O.S.;Son, K.S.;Kim, H.J.;Kim, I.H.
    • Asian-Australasian Journal of Animal Sciences
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    • v.19 no.4
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    • pp.587-592
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    • 2006
  • This study was conducted to evaluate the effects of supplementation with bacillus-based probiotic (Bacillus subtilis, $1.0{\times}10^7CFU/g$; Bacillus coagulans, $2.0{\times}10^6CFU/g$ and Lactobacillus acidophilus, $5.0{\times}10^6CFU/g$) on finishing pigs growth performance, nutrients digestibility, blood characteristics and fecal noxious gas content and to determine the optimal addition level of this probiotic preparation. A total of forty eight pigs with an initial body weight (BW) of $90.60{\pm}2.94kg$ were allotted to three dietary treatments (four pigs per pen with four pens per treatment) according to a randomized complete block design. Dietary treatment included: 1) CON (basal diet); 2) BP1 (basal diet+bacillus-based probiotic 0.1%) and 3) BP2 (basal diet+bacillus-based probiotic 0.2%). The experiment lasted 6 weeks. Through the entire experimental period, ADG was improved by 11% (p<0.05) in pigs fed diets supplemented with 0.2% bacillus-based probiotic compared to pigs fed the basal diet. ADFI and gain/feed were not affected by the treatments (p>0.05). Supplementation of bacillus-based probiotic did not affect either DM and N digestibilities or blood characteristics (p>0.05) of pigs. Fecal ammonia nitrogen ($NH_3$-N) measured at the end of experiment was reduced (p<0.05) when pigs were fed the diet with 0.2% bacillus-based probiotic. Fecal butyric acid concentration also decreased significantly (p<0.05) whereas acetic acid and propionic acid concentrations were not affected (p>0.05) when pigs were fed diets with added bacillus-based probiotic. In conclusion, dietary supplementation of bacillus-based probiotic can increase growth performance and decrease fecal noxious gas content concentration.

A Study on the develpoment of new recipes of 5-Korean Wild Vegetables (한국산 야생식용식물의 조리과학적 연구)

  • Lim, Sook-Ja;Park, North-Jung
    • Korean journal of food and cookery science
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    • v.10 no.4
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    • pp.412-419
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    • 1994
  • The Contents of proximate compositions, vitamin C and minerals(Ca, P, Fe and Zn) of five Korean wild vegetables were analyzed. Twenty four cooking recipes with three of the vegetables were developed and their taste characteristics were evaluated by 12-trained panels. The five Korean wild vegetables are Cassia tora(C.t.), Lycium chinese Mill(L.c.), Trichosanthes quadricirra MIQ(T.q.), Polygonatum japonicum MORR et DECAIS(P.j.) and Articum lappa L.(A.l.). They have been known as the efficacious plants agaginst diabetes mellitus. In our previous work, their hypoglycemic effects have been studied through streptozotocin-induced diabetic rats and fed on the wild vegetables. Contents of moisture were 14~86% in the samples and L.c. showed relatively higher content of crude protein(16.4%). High level of crude fat was seen in C.t.(9.4%). Contents of vitamin C were also relatively high in the wild vegetables(14.2~42.0 mg%). Analysis of minerals revealed the high level of Ca in L.c. leaves, P in A.l. and Fe in C.t. and L.c. leaves. All of the samples contained Zn in 5.0~14.6 mg%. Twelvetrained taste panels indicated that the 24-preparations of L.c. leaves, fruites and P.j. were well accepted in taste, color and odor. Although L.c. fruit gruel, L.c. cream soup and P.j. saute were evalulated as less acceptable, their sensory scores were in 3.6~5.4 which were not undesirable. The newly developed cooking recipes of L.c. fruits and leaves showed better scores in color and texture, P.j. in texture and odor.

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Effects of Iron on Arsenic Speciation and Redox Chemistry in Acid Mine Water

  • Bednar A.J.;Garbarino J.R.;Ranville J.F.;Wildeman T.R.
    • Proceedings of the KSEEG Conference
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    • 2004.12a
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    • pp.9-28
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    • 2004
  • Concern about arsenic is increasing throughout the world, including areas of the United States. Elevated levels of arsenic above current drinking-water regulations in ground and surface water can be the result of purely natural phenomena, but often are due to anthropogenic activities, such as mining and agriculture. The current study correlates arsenic speciation in acid mine drainage and mining influenced water with the important water-chemistry properties Eh, pH, and iron(III) concentration. The results show that arsenic speciation is generally in equilibrium with iron chemistry in low pH AMD, which is often not the case in other natural-water matrices. High pH mine waters and groundwater do not 짐ways hold to the redox predictions as well as low pH AMD samples. The oxidation and precipitation of oxyhydroxides depletes iron from some systems, and this also affects arsenite and arsenate concentrations differently through sorption processes.

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Study for the development of assembly type joint boxes for extra high voltage power cables (초고압 전력 케이블용 조립식 접속상의 개발을 위한 연구)

  • Seo, J.Y.;Park, J.S.;Lee, H.S.;Oh, E.J.;Han, B.S.;Hahn, K.M.;Lee, K.C.;Jeon, S.I.;Han, M.K.;Lee, J.H.;Shin, P.S.
    • Proceedings of the KIEE Conference
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    • 1992.07b
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    • pp.925-927
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    • 1992
  • Since that the portion of failures caused by mis-installation of accessories is about 50% of whole failures in power transmission lines, the necessity of assembly type joint boxes, which have excellent installation capability and which can be electrically tested before installation, is gradually increasing. In this paper, we presented the results from the study from the point of design and the results of experiments using models. With the results of study, we could make it clear that the possibility of practical use of assembly type joint boxes instead of conventional molding type joint boxes is very high.

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Variation of fibrinolytic enzyme activity produced Bacillus subtilis by gene cloning (유전자 cloning에 의한 Bacillus subtilis의 fibrinolytic enzyme 활성 변화)

  • 이홍석;유천권;이철수;강상모
    • Microbiology and Biotechnology Letters
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    • v.28 no.1
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    • pp.14-20
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    • 2000
  • The transformation of Bacillus subtilis K-54 and J-10 was carried out with constructed vectors containing structure and enhancer genes of aprN and prtR, to increase their fibrinolytic enzyme activity. Bands for the aprN and prtR genes were identified from B. subtilis J-10 by PCR that was carried out with the constructed primers for the genes. In addition, the gene fragments contained promoter site based on the results of analysing their nucleotide sequence. The two gene fragments, aprN and prtR, obtained by the PCR, were, then, inserted to vector such as T-vector and E.coli/Bacillus shuttle vector. The constructed vector were designated as pAPR2 (aprN), pENC2 (prtR) and pFLA1 (aprN and prtR), respectively. The constructed vector was used for transformation of the strains of B.subtilis J-10 and B. subtilis K-54 and the fribrinolytic activity of the transformed strains was investigated. The introduction of the vector, pAPR2 and the fibrinolytic activity of the transformed strains was investigated. The introduction of the vector, pAPR2 and pFLA1, resulted in the increase of fibrinolyitic enzyme activity in B. subtilis J-10 by 27.3% and 16%, respectively. However, the introduction of pENC2 to B. subtilis J-10 did not seem to induce increase of the enzyme activity. The strain of B.subtilis K-54 transformed with pENC2 showed an increased fibrinolytic activity by 5 folds compared with that of the original strain of B. subtilis K-54.

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Isolation and Characterization of Peroxidase from Jerusalem Artichoke Tubers (돼지감자 Peroxidase의 분리와 특성)

  • Yoon, Eun-Seok;Kang, Su-Jung;Noh, Bong-Soo;Choi, Eon-Ho
    • Korean Journal of Food Science and Technology
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    • v.25 no.5
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    • pp.565-570
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    • 1993
  • Peroxidase from Jerusalem artichoke tubers, which might be related to browning reaction, was purified by ammonium sulfate precipitation, DEAE-cellulose and Sephacryl S-200 chromatography. The optimum pH of the purified peroxidase was 5.0 and relatively stable at pH $5.0{\sim}6.0$ using substrate of p-phenylenediamine and $H_2O_2$. D-values for thermal inactivation at 60, 70 and $80^{\circ}C$ were 86, 45 and 33 sec, respectively. Activation energy was 4,111 J/mole. The enzyme showed the most sensitive specificity of substrate for p-phenylenediamine. The compounds such as 1mM potassium cyanide, 10mM sodium diethyldithiocarbamate, L-ascorbic acid, sodium hydrosulfite and L-cysteine inhibited completely while 1mM of $Ca^{2+}\;and\;Cu^{2+}$ activated the purified peroxidase.

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MONOTONICITY PROPERTIES OF THE GENERALIZED STRUVE FUNCTIONS

  • Ali, Rosihan M.;Mondal, Saiful R.;Nisar, Kottakkaran S.
    • Journal of the Korean Mathematical Society
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    • v.54 no.2
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    • pp.575-598
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    • 2017
  • This paper introduces and studies a generalization of the classical Struve function of order p given by $$_aS_{p,c}(x):=\sum\limits_{k=0}^{\infty}\frac{(-c)^k}{{\Gamma}(ak+p+\frac{3}{2}){\Gamma}(k+\frac{3}{2})}(\frac{x}{2})^{2k+p+1}$$. Representation formulae are derived for $_aS_{p,c}$. Further the function $_aS_{p,c}$ is shown to be a solution of an (a + 1)-order differential equation. Monotonicity and log-convexity properties for the generalized Struve function $_aS_{p,c}$ are investigated, particulary for the case c = -1. As a consequence, $Tur{\acute{a}}n$-type inequalities are established. For a = 2 and c = -1, dominant and subordinant functions are obtained for the Struve function $_2S_{p,-1}$.

In vitro cytotoxicity and in vivo acute toxicity of selected polysaccharide hydrogels as pharmaceutical excipients

  • Kulkarni GT;Gowthanarajan K;Raghu C;Ashok G;Vijayan P
    • Advances in Traditional Medicine
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    • v.5 no.1
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    • pp.29-36
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    • 2005
  • Polysaccharide hydrogels constitute a structurally diverse class of biological macromolecules with a wide range of physicochemical properties. They also constitute important members of the family of industrial water-soluble polymers. They find application in Pharmacy as binders, disintegrants, suspending, emulsifying and sustaining agents. According to the International Pharmaceutical Excipients Council (IPEC), an excipient must have an established safety profile. Hence, in the present study, in vitro cytotoxicity on Vero and HEp-2 cell lines, and in vivo acute toxicity in rats were carried out to establish the safety of polysaccharide hydrogels from the seeds of Plantago ovata and Ocimum basilicum. The in vitro cytotoxicity was determined by MTT and SRB assays. In the in vivo acute toxicity, the effects of three different doses of hydrogels (100, 200 and 400 mg/kg body weight) on food and water intake, body weight, biochemical and hematological parameters were studied. The results of in vitro did not show any cytotoxicity on both the cell lines used. In the in vivo acute toxicity, the hydrogels did not show any toxic symptoms in all three dose levels. This establishes the safety of the selected hydrogels. Hence, they can be used as excipients in pharmaceutical dosage forms.

Analysis of Harmonic Effect of Residential Loads for Load Modeling (부하모델을 위한 주거용 부하의 고조파 영향 분석)

  • Ji, P.S.;Lim, J.T.;Lee, D.J.;Lee, J.P.;Park, J.W.;Lim, J.Y.
    • Proceedings of the KIEE Conference
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    • 2008.07a
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    • pp.199-200
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    • 2008
  • 산업의 발달과 함께 전력계통에는 첨단 제어장치, 전력전자 기기로 대표되는 다양한 반도체 전력설비와 전력기기인 변압기, 회전기기 등의 비선형 특성을 나타내는 부하설비의 사용이 증가하고 있다. 이러한 비선형 부하들은 고조파를 발생시켜 입력전압의 왜곡뿐만 아니라 연계된 계통내로 고조파 전류가 흘러 들어가 다른 부하설비에 까지 영향을 미친다. 따라서 본 논문에서는 고조파가 포함된 조건하에서 부하모델링에 필수 요소인 유효전력과 무효전력에 대한 특성을 주거용 부하를 대상으로 실험하여 고조파에 대한 주거용 부하의 특성을 분석하고자한다.

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GEOUNED: A new conversion tool from CAD to Monte Carlo geometry

  • J.P. Catalan;P. Sauvan;J. Garcia;J. Alguacil;F. Ogando;J. Sanz
    • Nuclear Engineering and Technology
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    • v.56 no.6
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    • pp.2404-2411
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    • 2024
  • The GEOUNED code is specifically designed to convert CAD models, defined using the B-rep approach, into MC radiation transport models, defined using the CSG approach, and vice versa from MC to CAD. This code incorporates standard features commonly found in conversion tools, including decomposition, conversion, and automatic void generation. Additionally, it introduces innovative features, mainly in the automatic void generation part, which are described in this article. GEOUNED has demonstrated successful application in highly detailed 3D models used in fusion neutronics, which are known for their complex geometries, particularly those utilized in ITER. The article includes examples showcasing GEOUNED's performance in these challenging models, as well as custom applications that highlight its flexibility in addressing non-standard problems. The code is open-source and utilizes Open CASCADE as the geometry engine, with FreeCAD serving as the Python API.