• 한국통신학회논문지
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• 제37B권12호
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• pp.1148-1159
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• 2012

사용자가 사용할 수 있는 단말이 다수 개가 되고, 수많은 단말들이 네트워크에 연결되는 유비쿼터스 네트워크 환경을 지원하는 NGN에서는 단말 이동성 지원 뿐 만 아니라 서비스 연속성 지원 또한 매우 중요하다. NGN에서는 기본 전화 서비스 뿐 만 아니라 다양한 멀티미디어 서비스를 IMS를 통해 사용자에게 제공한다. NGN에서의 서비스 연속성 지원을 위해 현재의 IMS에서도 단말 간 진행 중인 세션을 이동할 수 있는 이른 바 IUT(Inter-UE Transfer) 기능을 제공하고 있다. 그러나 IMS 서비스 연속성 제공 기능은, 현재 세션을 유지하고 있는 단말 및 세션을 이동할 목표 단말이 모두 같은 사용자의 단말이어야 한다는 한계가 존재한다. 본 논문에서는 이러한 문제를 극복하기 위하여 단말 디스커버리 기능과 IMS UE의 기능을 확장하였고, IMS 확장이 기존 방법에 비하여 얼마나 오버헤드가 생길 것인지 분석하였다.

• Journal of Microbiology and Biotechnology
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• 제19권12호
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• pp.1635-1643
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• 2009

The aim of this study was to provide new insight into the mechanism whereby the housekeeping enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH) locates to cell walls of Lactobacillus plantarum 299v. After purification, cytosolic and cell wall GAPDH (cw-GAPDH) forms were characterized and shown to be identical homotetrameric active enzymes. GAPDH concentration on cell walls was growth-time dependent. Free GAPDH was not observed on the culture supernatant at any time during growth, and provoked cell lysis was not concomitant with any reassociation of GAPDH onto the cell surface. Hence, with the possibility of cw-GAPDH resulting from autolysis being unlikely, entrapment of intracellular GAPDH on the cell wall after a passive efflux through altered plasma membrane was investigated. Flow cytometry was used to assess L. plantarum 299v membrane permeabilization after labeling with propidium iodide (PI). By combining PI uptake and cw-GAPDH activity measurements, we demonstrate here that the increase in cw-GAPDH concentration from the early exponential phase to the late stationary phase is closely related to an increase in plasma membrane permeability during growth. Moreover, we observed that increases in both plasma membrane permeability and cw-GAPDH activity were delayed when glucose was added during L. plantarum 299v growth. Using a double labeling of L. plantarum 299v cells with anti-GAPDH antibodies and propidium iodide, we established unambiguously that cells with impaired membrane manifest five times more cw-GAPDH than unaltered cells. Our results show that plasma membrane permeability appears to be closely related to the efflux of GAPDH on the bacterial cell surface, offering new insight into the understanding of the cell wall location of this enzyme.

• 한국정보처리학회논문지
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• 제4권11호
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• pp.2832-2839
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• 1997

본 연구에서는 Asynchronous Transfer Mode (ATM) 계층의 기능중 유휴 가상 채널에 프로토콜 프로토콜 적합성 시험 제어 기능을 갖게 하여 Test Coordination Procedures (TCP) 채널로 활용하는 방안을 제시하였다. 프로토콜 적합성 시험을 위하여 국제 기구에서 권고하고 있는 방법중 원격 시험 방법을 선택하면 System Under Test (SUT)측에 서 능동적으로 Test Event를 발생시켜야 하는 경우에 개발자가 직접 수동적으로 운용을 하거나, Implementation Under Test (IUT)에 대한 제어 및 관찰이 불가능한 경우도 발생하게 된다. ATM망에서 프로토콜 적합성 시험시 시험 영역(Test Coverage)을 최대화하고, 오류검출 영역(Fauit Coverase)을 극대화하기 위해서는 원격시험방법보다는 분산시험방법이 적절하며, 분산시험방법을 채택할 경우 TCP를 구현하기 위하여 시험정보를 전달하기 위한 채널을 확보하는 것이 요구된다. 본 고에서 제안하고 있는 방법은 오류 검출 영역을 극대화 시키고 오퍼레이터의 개입없이 시험의 자동화 구현이 용이하고, ATM장치의 정상동작에는 영향을 주지 않음은 물론 시험제어를 위한 별도의 채널을 확보하지 않고서도 분산시험방법을 적용할 수 있다는 점 을 특징으로 들 수 있다．

• 한국정보처리학회논문지
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• 제4권5호
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• pp.1211-1220
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• 1997

다수 공급에 의해 제공되는 여러 구현들간의 상호운용성 시험은 비용을 초래할 뿐아니라 실제 상황에서 때로는 그 자체가 불가능하다. 적합성 시험이나 적합성 시험을 확정한 방법들 만으로는 효과적으로 상호운용성을 보장할 수 없다 본 논문 에서는 실시간 시스템인 통신망의 구성 요소들을 매우 훌륭하게 추상화 할 수 있는 실시간 객체 모형을 기반으로 한 프로토콜 상호운용성 시험 방법을 제시한다. 이 방법 에서는 RTO.k 객체 모형을 이용하여 IUT(lmplementation Under Test)와 시험 환경을 추상화하고 그 결과 얻어진 객체들을 실행 환경인 DREAM 커널 상에 지역 모니터와 전역 모니터를 두어 수행시킨다. 지역 모니터는 관찰과 제어를 주요 임무로 하고 전역 모니터는 시험 과정을 주관한다. 시험 방법의 검증을 위하여 B-ISDN UNI Q.2931 의 예를 들어 호 연결 제어 프로토콜 상호운용성 시험을 실험야 하였다. 이 방법으로 높은 결함 발견율을 얻을 수 있다.

• 한국정보전자통신기술학회논문지
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• 제6권3호
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• pp.135-140
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• 2013

본 논문은 스마트 그리드의 핵심 설비중 하나인 반도체 지능형 변압기의 시스템 구성과 이에 필요한 PWM 컨버터의 PWM 생성 방식에 관한 논문으로, 반도체 지능형 변압기의 경우 입력단이 수전계통에 직접 접속되기 때문에 고전압을 견딜 수 있는 방식으로 구성되어야 하고 이를 위해서는 다단 연결방식의 PWM 컨버터의 구성이 필수적이다. 종래의 PWM 생성 방식의 경우 지령전압의 크기에 따라서 각 단별 스위칭 부하가 균등하게 분포되지 않기 때문에 이를 해결하기 위한 복잡한 리던던시를 스위칭 패턴에 추가해야 했으나, 본 논문에서 제안하는 방식의 경우 부하의 분담이 자동적으로 이루어지기 때문에 그러한 부담없이 시스템을 구성하는 것이 가능하다.

• 대한수의학회지
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• 제53권4호
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• pp.199-205
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• 2013

Avian pathogenic Escherichia coli (APEC) is a causative agent for a number of extra intestinal diseases and account for significant losses to the poultry industry. Since protective immunity against APEC is largely directed to virulence antigens, we have individually expressed four different viulence antigens, papA, papG, IutA, and CS31A, using an attenuated Salmonella Typhimurium and a plasmid pBB244. Following oral immunization of mice with combination of two or four of these strains, serum IgG and mucosal IgA responses were elicited against each antigen represented in the mixture. The antigen-specific mucosal IgA responses were significantly higher in the group of mice immunized with the heat-labile Escherichia coli enterotoxin B subunit (LTB) strain than those in the group of mice immunized without the LTB strain. While, there was no significant difference between these two groups in antigen-specific serum IgG responses. The results showed that LTB could act as mucosal immune adjuvant. To assess the nature of immunity, the distribution of antigen-specific IgG isotypes was analyzed. All groups promoted Th1-type immunity as determined by the IgG2a/IgG1 ratio. Thus, our findings provided evidence that immunization with a combination of several vaccine strains is one of the strategies of developing effective vaccines against APEC.

• Advances in materials Research
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• 제2권3호
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• pp.155-172
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• 2013

The study of the corrosion inhibition of armatures made of steel conceived for reinforced concrete by sodium phosphate is the aim object of our experimental tests. Gravimetric and electrochemical measurements were carried in three different Mediums contaminated by chlorides (3% NaCl) with addition of increasing concentrations of sodium phosphate. Inhibitory efficiency reached 80% at an optimal concentration of $7,5{\times}10^{-3}M$, the results obtained using the gravimetric measurements are in good agreement with those obtained by electrochemical methods. However, the monitoring of the pH evolution after 24h shows in the three studied environments, that the pH decreases slightly at 24 hours from the initial pH at $t_0$, due to the presence of corrosion products which change the state of the final solution. Also, scanning electron microscopy revealed the existence of layers of apatite on the metal surface previously treated with the sodium phosphate which confirms the formation of a protective film around the surface of the metal.

• Steel and Composite Structures
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• 제6권5호
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• pp.401-415
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• 2006

Based on a non-linear model taking into account flexural-torsional couplings, analytical solutions are derived for lateral buckling of simply supported I beams under some representative load cases. A closed form is established for lateral buckling moments. It accounts for bending distribution, load height application and pre-buckling deflections. Coefficients $C_1$ and $C_2$ affected to these parameters are then derived. Regard to well known linear stability solutions, these coefficients are not constant but depend on another coefficient $k_1$ that represents the pre-buckling deflection effects. In numerical simulations, shell elements are used in mesh process. The buckling loads are achieved from solutions of eigenvalue problem and by bifurcations observed on non linear equilibrium paths. It is proved that both the buckling loads derived from linear stability and eigenvalue problem lead to poor results, especially for I sections with large flanges for which the behaviour is predominated by pre-buckling deflection and the coefficient $k_1$ is large. The proposed solutions are in good agreement with numerical bifurcations observed on non linear equilibrium paths.

• Advances in concrete construction
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• 제5권5호
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• pp.539-561
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• 2017

In this study, the effects of initial damage of concrete columns on the post-repair performance of reinforced concrete (RC) columns strengthened with carbon-fiber-reinforced polymer (CFRP) composite are investigated experimentally. Four kinds of compression-damaged RC cylinders were reinforced using external CFRP composite wraps, and the stress-strain behavior of the composite/concrete system was investigated. These concrete cylinders were compressed to four pre-damaged states including low -level, medium -level, high -level and total damage states. The percentages of the stress levels of pre-damage were, respectively, 40, 60, 80, and 100% of that of the control RC cylinder. These damaged concrete cylinders simulate bridge piers or building columns subjected to different magnitudes of stress, or at various stages in long-term behavior. Experimental data, as well as a stress-strain model proposed for the behavior of damaged and undamaged concrete strengthened by external CFRP composite sheets are presented. The experimental data shows that external confinement of concrete by CFRP composite wrap significantly improves both compressive strength and ductility of concrete, though the improvement is inversely proportional to the initial degree of damage to the concrete. The failure modes of the composite/damaged concrete systems were examined to evaluate the benefit of this reinforcing methodology. Results predicted by the model showed very good agreement with those of the current experimental program.

• Journal of Veterinary Science
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• 제23권1호
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• pp.9.1-9.11
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• 2022

Background: Escherichia coli, which causes subclinical or clinical mastitis in cattle, is responsible for transmitting antimicrobial resistance via human consumption of raw milk or raw milk products. Objectives: The objective of this study was to investigate the molecular characteristics of 183 E. coli from bulk tank milk of five different dairy factories in Korea. Methods: The molecular characteristics of E. coli such as serogroup, virulence, antimicrobial resistance, and integron genes were detected using polymerase chain reaction and antimicrobial susceptibility were tested using the disk diffusion test. Results: In the distribution of phylogenetic groups, group D was the most prevalent (59.6%) and followed by group B1 (25.1%). The most predominant serogroup was O173 (15.3%), and a total of 46 different serotypes were detected. The virulence gene found most often was fimH (73.2%), and stx1, fimH, incC, fyuA, and iutA genes were significantly higher in isolates of phylogenetic group B1 compared to phylogenetic groups A, B2, and D (p < 0.05). Among 64 E. coli isolates that showed resistance to at least one antimicrobial, the highest resistance rate was observed for tetracyclines (37.5%). All 18 integron-positive E. coli carried the integron class I (int1) gene, and three different gene cassette arrangements, dfrA12+aadA2 (2 isolates), aac(6')-Ib3+aac(6')-Ib-cr+aadA4 (2 isolates), and dfrA17+aadA5 (1 isolate) were detected. Conclusions: These data suggest that the E. coli from bulk tank milk can be an indicator for dissemination of antimicrobial resistance and virulence factors via cross-contamination.