• Journal of The Korean Society of Grassland and Forage Science
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• v.39 no.3
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• pp.127-131
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• 2019

The effects of lactic acid bacteria (LAB) mixtures on low moisture Italian ryegrass (IRG) silage fermentation was evaluated in field conditions. The experiment was categorized into two groups: Un-inoculated (Control) and Inoculated with LAB mixture for four storage periods (45, 90, 180, and 365 days, respectively). Silage inoculated with the LAB mixture had the lowest pH with highest lactic acid production than the control from beginning at 45-365 days at all moistures. Higher LAB counts were observed in inoculated silages than the control silages at whole experimental periods. It is a key reason for the rapid acidification and higher lactic acid production in silages during the storage periods. Overall results suggest that an adding of LAB mixture had positive effects on the increasing aerobic stability of silage and preserved its quality for an extended duration.

• Journal of The Korean Society of Grassland and Forage Science
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• v.39 no.3
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• pp.158-164
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• 2019

We examined the adaptability of Korean winter forage crops such as Italian ryegrass, barley, oat, and triticale in Uzbekistan. The overall growth before wintering was slightly different between Uzbekistani and Korean varieties, but there was no significant difference among winter forage crops. The heading date of the Korean triticale 'Shinyoung' was the earliest, (April 4), and the heading date of the Uzbekistani triticale 'Frack cerebristy' was quite late, (April 25). The heading date of the Korean Italian ryegrass 'Kowinearly' was four days earlier than that of 'Kowinmaster,' on April 13, and that of the Korean barley 'Youngyang' and 'Yuyeon' were five days earlier than that of the Uzbekistani barley 'Bolgaly'. The maximum dry matter yield of winter forage crop was from Uzbekistan triticale, 'Frack cerebristy', which was 13,536 kg/ha. The dry matter yield of Uzbekistan barley was 8,222 kg/ha. Compared with Uzbekistan barley, 'Bolgaly', yields of Korean barley 'Youngyang' and 'Yuyeon', Korean oat 'Samhan', and Korean Italian ryegrass 'Kowinearly' and 'Kowinmaster' were 59~64%, 125%, and 113~133%, respectively.

• Journal of The Korean Society of Grassland and Forage Science
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• v.44 no.3
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• pp.165-172
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• 2024

Italian ryegrass (IRG) has become a vital forage crop due to its increasing cultivation area and its role in enhancing forage self-sufficiency. However, its production is susceptible to environmental factors such as climate change and drought, necessitating precise yield prediction technologies. This study aimed to assess the growth characteristics of IRG and predict dry matter yield (DMY) using vegetation indices derived from unmanned aerial vehicle (UAV)-based remote sensing. The Green Leaf Index (GLI), normalized difference vegetation index (NDVI), normalized difference red edge (NDRE), and optimized soil-adjusted vegetation index (OSAVI) were employed to develop DMY estimation models. Among the indices, GLI demonstrated the highest correlation with DMY (R² = 0.971). The results revealed that GLI-based UAV observations can serve as reliable tools for estimating forage yield under varying environmental conditions. Additionally, post-winter vegetation coverage in the study area was assessed using GLI, and 54% coverage was observed in March 2023. This study assesses that UAV-based remote sensing can provide high-precision predictions of crop yield, thus contributing to the stabilization of forage production under climate variability.

• Korean Journal of Agricultural Science
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• v.45 no.1
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• pp.84-93
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• 2018

This study was conducted to develop a pasture feeding program for reducing the Hanwoo feeding cost. Italian ryegrass (IRG) is a major plant which is abundantly produced in the southern area of South Korea. Locally produced Italian ryegrass has a 30 - 50% reduced pasture cost compared to imported pasture grasses. We hypothesized that Italian ryegrass feed could reduce the feeding cost but not change the physiological characteristics of the Hanwoo steers in the feedlot. Four treatments (Control, short fed IRG silage, long fed IRG silage, and IRG hay) in a completely random design were used to feed 27 Hanwoo steers. Three or four steers were fed in the same pen, and 8 pens were used for the treatments. Blood was drawn from each steer on the first week of every month from 11 to 30 months. Overall, the average daily gain and feed efficiency were not different among the treatments (p < 0.05) except for the final fattening diets (p < 0.05). The long fed IRG silage group had a low ADG compared to the other groups (p < 0.05). The serum non-esterified fatty acid and glucose concentrations were reduced during the fattening periods (p < 0.05); however, the serum albumin, total proteins, triglycerides, and phosphorus were increased during the fattening periods (p < 0.05). The carcass weight, longissimus muscle area and back-fat thickness were low in the long fed IRG silage group (p < 0.05). The IRG diet induced saturated fatty acids in the longissimus dorsi (p < 0.05). These results show that the IRG diet increased the serum triglycerides but reduced the glucose level at the final fattening diet and decreased the meat yield score in Hanwoo steers.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.42 no.1
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• pp.104-111
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• 1997

Since seeds of Italian ryegrass should be imported every year, it is necessary to investigate the prossible production of seeds in Italian ryegrass field after rice. Seven Italian ryegrass varieties were planted on October 8, 1986 at National Honam Crop Experiment Station at Iksan and clippings were made on April 10 and April 30 in 1987. Headings of K-11 and T.N.T were earlier than those of Tetrone and Bettina. For all varieties tested, heading date was delayed and 1,000 grain weight was decreased linearly as the clipping was made later. Length, width and thickness of a grain were reduced by clipping and later clipping, while tetraploid varieties produced larger grains than diploid varieties. The germination percentage and germination speed at $25^{\circ}C$ were higher than at 15$^{\circ}C$. The final germination percentage was lower at the later clippings and was higher at 15$^{\circ}C$, and earlier varieties showed higher germination percentage. Uniformity of germination was greater at $25^{\circ}C$ and was decreased as the clipping was made later, and the eariler varieties such as K-11 were germinated more uniformly. Average number of days to germination was longer at 15$^{\circ}C$ than at $25^{\circ}C$ and was longer at later clipping. The germination percentage was decreased linearly as the heading was delayed, while it increased linearly as the 1,000 grain weight was increased.

• Asian-Australasian Journal of Animal Sciences
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• v.11 no.3
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• pp.277-284
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• 1998

This experiment was carried out to study the effects of lactic acid bacteria (LAB) inoculation and addition of cell wall degrading enzymes on the fermentation characteristics and chemical compositions of Italian ryegrass silage. An inoculant LAB with or without a cell wall degrading enzyme of Acremoniumcellulase (A), or Meicellulase (M) or a mixture of both (AM), was applied to 1 kg of fresh Italian ryegrass sample. The treatments were control untreated, LAB-treated (application rate $10^5$ cfu/g fresh sample), LAB+A 0.005%, LAB + A 0.01%, LAB+A 0.02%, LAB + M 0.005%, LAB + M 0.01%, LAB + M 0.02%, LAB+AM 0.005%, LAB + AM 0.01% and LAB+AM 0.02%. The sample was ensiled into 2-L vinyl bottle silo, with 9 silages of each treatment were made (a total of 99 silages). Three silages of each treatment were incubated at 20, 30 and $40{^{\circ}C}$ for an approximately 2-months storage period. All silages were well preserved as evidenced by their low pH values (3.79-4.20) and high lactic acid concentrations (7.71-11.34% DM). The fermentation quality and chemical composition of the control untreated and the LAB-treated silages were similar, except that for volatile basic nitrogen (VBN) content was lower (p < 0.05) in the LAB-treated silages. LAB + cellulase treatments improved the fermentation quality of silages by decreasing (p < 0.01) pH values and increasing (p<0.01) lactic acid concentrations, in all of cellulase types and incubation temperatures. Increasing amount of cellulase addition resulted in further decrease (p < 0.01) of pH value and increases (p < 0.01) of lactic acid and residual water soluble carbohydrate (WSC) concentrations. LAB + cellulase treatments reduced (p<0.01) NDF, ADF, hemicellulose and cellulose contents of silages compared with both the control untreated and LAB-treated silages. LAB + cellulase treatments did not affect the silage digestibility due to fact of in vitro dry matter digestibility (IVDMD) was similar in all silages. The silages treated with cellulase A resulted in a better fermentation quality and a higher rate of cell wall reduction losses than those of the silages treated with cellulases M and AM. Incubation temperature of $30{^{\circ}C}$ seemed to be more suitable for the fermentation of Italian ryegrass silages than those of 20 and $40{^{\circ}C}$.

• Journal of The Korean Society of Grassland and Forage Science
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• v.29 no.4
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• pp.313-320
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• 2009

This experiment was conducted to evaluate the effect of tillage system and fertilizer type on the forage yield and quality of Italian ryegrass. This experiment was a $2{\times}2$ factorial arrangement for two tillage systems (tillage and no-tillage) and two fertilizer type (chemical and manure compost). The plant height, dry matter (DM) content, DM yield, TDN (total digestible nutrients) yield, and CP (crude protein) yield have significant differences in tillage system of Italian ryegrass. Italian ryegrass cultivated with tillage (plow and rotary till) had lower plant height, DM yield, TDN yield and CP yield than no-tillage, while its DM content showed the opposite results. However, there were no significant differences in the results on fertilizer types. Neutral detergent fiber (NDF) and acid detergent fiber (ADF) contents were significantly higher in no-tillage compared to tillage. However, there was no significant difference in CP content on tillage system. TDN and relative feed value (RFV) were higher in tillage than no-tillage system (p<0.01). The TDN content was lower when using manure as fertilizer. No significant effects were observed for NDF and RFV in fertilizer type of Italian ryegrass. Based on the results of this study, tillage system and fertilizer type affected forage quality as well as forage yield. Forage yield of no-tillage was higher compared to tillage.

• Journal of The Korean Society of Grassland and Forage Science
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• v.37 no.2
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• pp.183-188
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• 2017

This study investigated the comparative growth characteristics, yield, chemical compositions, and nutritive yield of Italian ryegrass cultivars harvested in a paddy field. The experimental design was a randomized block design with three replicates. The treatments consisted of seven Italian ryegrass cultivars (Kogreen, Kowinmaster, Hwasan 101, Kowinnearly, Kospeed, Sahalie select, and Strike). The planting date was October 21, 2009 and the sample harvest was occurred on May 21, 2010. Dry matter yield was higher in Kowinnerary and Kogreen than in the other varieties (p<0.05). Crude protein and ether extract contents were highest in Hwasan 101 and lowest in Kospeed. The content of total digestible nutrients did not differ among the Italian ryegrass varieties. The amino acid contents were in the order Hwasan 101 > Strike > Kowinnearly > Kogreen > Sahalie select > Kospeed > Kowinnearly (p<0.05). Fructose content was highest in Kospeed, while glucose and sucrose contents were highest in Hwasan 101. The results of this study indicate that Kowinnearly, in terms of dry matter yield, and Hwasan 101, in terms of feed value (chemical composition and free sugar content), may be recommended for forage production in a paddy field.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.67 no.2
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• pp.131-136
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• 2022

This study was conducted to evaluate the efficacy and phytotoxic of crop desiccant treatments on the seed production of Italian ryegrass (Lolium multiflorum Lam.) in Gimje and Buan, South Korea. Here, Italian ryegrass was treated 7, 10, and 14 days before harvest (DBH) and no difference in fresh weight, 1000-seed weight, germination rate, and seed yield was observed compared to the untreated control in the standard dose and double dose for seven DBH. However, the standard dose and double dose on the 10 DBH and 14 DBH had a lower 1000-seed weight, germination rate, and seed yield compared to the untreated control. In pesticide persistence analysis, the permitted standards were not exceeded in the seeds and straw according to the treatment time and concentration. These results suggest that the treatment effect and stability of Italian ryegrass can be expected if treated within seven DBH. However, it seems that crop desiccants should be used after considering grain ripening and weather to minimize loss of seeds.

• Journal of The Korean Society of Grassland and Forage Science
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• v.38 no.3
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• pp.190-195
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• 2018

Cold, salt and heat are the most critical factors that restrict full genetic potential, growth and development of crops globally. However, clarification of genes expression and regulation is a fundamental approach to understanding the adaptive response of plants under unfavorable environments. In this study, we applied an annealing control primer (ACP) based on the GeneFishing approach to identify differentially expressed genes (DEGs) in Italian ryegrass (cv. Kowinearly) leaves under cold, salt and heat stresses. Two-week-old seedlings were exposed to cold ($4^{\circ}C$), salt (NaCl 200 mM) and heat ($42^{\circ}C$) treatments for six hours. A total 8 differentially expressed genes were isolated from ryegrass leaves. These genes were sequenced then identified and validated using the National Center for Biotechnology Information (NCBI) database. We identified several promising genes encoding light harvesting chlorophyll a/b binding protein, alpha-glactosidase b, chromosome 3B, elongation factor 1-alpha, FLbaf106f03, Lolium multiflorum plastid, complete genome, translation initiation factor SUI1, and glyceraldehyde-3-phosphate dehydrogenase. These genes were potentially involved in photosynthesis, plant development, protein synthesis and abiotic stress tolerance in plants. However, this study provides new insight regarding molecular information about several genes in response to multiple abiotic stresses. Additionally, these genes may be useful for enhancement of abiotic stress tolerance in fodder crops as well a crop improvement under unfavorable environmental conditions.