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Lactic Acid Bacteria Mixture as Inoculants on Low Moisture Italian Ryegrass Silage Fermentation

  • Soundharrajan, Ilavenil (Grassland and Forage Division, National Institute of Animal Science, RDA) ;
  • Kuppusamy, Palaniselvam (Grassland and Forage Division, National Institute of Animal Science, RDA) ;
  • Park, Hyung Soo (Grassland and Forage Division, National Institute of Animal Science, RDA) ;
  • Kim, Ji Hea (Grassland and Forage Division, National Institute of Animal Science, RDA) ;
  • Kim, Won Ho (Grassland and Forage Division, National Institute of Animal Science, RDA) ;
  • Jung, Jeong Sung (Grassland and Forage Division, National Institute of Animal Science, RDA) ;
  • Choi, Ki Choon (Grassland and Forage Division, National Institute of Animal Science, RDA)
  • Received : 2019.08.06
  • Accepted : 2019.09.10
  • Published : 2019.09.30

Abstract

The effects of lactic acid bacteria (LAB) mixtures on low moisture Italian ryegrass (IRG) silage fermentation was evaluated in field conditions. The experiment was categorized into two groups: Un-inoculated (Control) and Inoculated with LAB mixture for four storage periods (45, 90, 180, and 365 days, respectively). Silage inoculated with the LAB mixture had the lowest pH with highest lactic acid production than the control from beginning at 45-365 days at all moistures. Higher LAB counts were observed in inoculated silages than the control silages at whole experimental periods. It is a key reason for the rapid acidification and higher lactic acid production in silages during the storage periods. Overall results suggest that an adding of LAB mixture had positive effects on the increasing aerobic stability of silage and preserved its quality for an extended duration.

Keywords

References

  1. AOAC.1990. Official method of analysis. 15thed. Washington, DC.
  2. Arasu, M.V., Jung, M.W., Kim, D.H., Ilavenil, S., Jane, M., Park, H.S., Al-Dhabi, N.A., Jeon, B.T., and Choi, K.C. 2014. Enhancing Nutritional Quality of Silage by Fermentation with Lactobacillus plantarum. Indian Journal of Microbiology. 54:396-402. https://doi.org/10.1007/s12088-014-0473-9
  3. Borreani, G., Tabacco, E., Schmidt, R.J., Holmes, B.J., and Muck, R.E. 2018. Silage review: Factors affecting dry matter and quality losses in silages. Journal of Dairy Science. 101:3952-3979. https://doi.org/10.3168/jds.2017-13837
  4. Danner, H., Holzer, M., Mayrhuber, E., and Braun, R. 2003. Acetic acid increases stability of silage under aerobic conditions. Applied and Environmental Microbiology. 69:562-567. https://doi.org/10.1128/AEM.69.1.562-567.2003
  5. Davies, D.R., Theodorou, M.K., Kingston-Smith, A.H. and Merry, R.J. 2005. Advances in silage quality. Pages International Silage Conference. Proc. Belfast, Northern Ireland. pp. 121-133.
  6. Dietl, W. and Lehmann, J. 2004. Okologischer Wiesenbau. Nachhaltige Bewirtschaftung von Wiesen und Weiden. Osterreichischer Agrarverlag, Leopoldsdorf.
  7. Driehuis, F., Oude Elferink, S.G. and Van Wikselaar, P. 2001. Fermentation characteristics and aerobic stability of grass silage inoculated with Lactobacillus buchneri, with or without homofermentative lactic acid bacteria. Grass and Forage Science. 56:330-343. https://doi.org/10.1046/j.1365-2494.2001.00282.x
  8. Eisner, I., Sudekum, K.H., and Kirchhof, S. 2006. Relationships between silage fermentation characteristics and feed intake by dairy cows. Ubers. Tierernahr. 34:197-221.
  9. Han, J.K., Alison, M.D., Pitman, W.E. and McCormick, M. 2013. Contribution of Field Pea to Winter Forage Production and Nutritive Value in the South-Central United States. Crop Science. 53:315. https://doi.org/10.2135/cropsci2012.04.0260
  10. Hides, H.D.A., Lovatt, J.V. and Hayward, M. 2006. Influence of stage of maturity on the nutritive value of Italian ryegrass. Grass and Forage Science. 38:33-38. https://doi.org/10.1111/j.1365-2494.1983.tb01619.x
  11. Ilavenil, S., Kim, D.H., Srisesharam, S., Kuppusamy, P., Park, H.S., Yoon, Y.H., Kim, W.H., Song, Y.G. and Choi, K.C. 2017. Application of customised bacterial inoculants for grass haylage production and its effectiveness on nutrient composition and fermentation quality of haylage. 3 biotech. 7:321.
  12. Kim, H., Hyun, S. and Kim, K.S. 2014. A Study on the Prediction of Suitability Change of Forage Crop Italian Ryegrass (Lolium multiflorum L.) using Spatial Distribution Model. Korean Journal of Agricultural and Forest Meteorology. 16:103-113. https://doi.org/10.5532/KJAFM.2014.16.2.103
  13. Kim, K.D., Sung, K.I., Jung, Y.S., Lee, H.I., Kim, E.J., Nejad, J.G., Jo, M.H. and Lim, Y.C. 2012. Suitability classes for italian ryegrass (Lolium multiflorum Lam.) using soil and climate digital database in Gangwon Province. The Korean Society of Grassland and Forage Science. 32:437-446. https://doi.org/10.5333/KGFS.2012.32.4.437
  14. Kleinschmit, D.H. and Kung Jr, L. 2006. A meta-analysis of the effects of Lactobacillus buchneri on the fermentation and aerobic stability of corn and grass and small-grain silages. Journal of dairy science. 89:4005-4013. https://doi.org/10.3168/jds.S0022-0302(06)72444-4
  15. Kung Jr, L., Shaver, R.D., Grant, R.J. and Schmidt, R.J. 2018. Silage review: Interpretation of chemical, microbial, and organoleptic components of silages. Journal of Dairy Science. 101:4020-4033. https://doi.org/10.3168/jds.2017-13909
  16. Kung Jr, L., Stokes, M.R. and Lin, C.J. 2003. Silage additives, Buxton D.R., Muck, R.E. and Harrison, H.J. ed. American Society of Agronomy, Madison, WI.
  17. Kung, L. and Shaver, R. 2001. Interpretation and Use of Silage Fermentation Analysis Reports, Focus on Forage, University of Wisconsin Extension.
  18. McDonald, P. 1981. The Biochemistry of Silage. J. Wiley.
  19. Muck, R.E. 2010. Silage microbiology and its control through additives Revista Brasileria de Zootecnia. 39:183-191. https://doi.org/10.1590/S1516-35982010001300021
  20. Pahlow, G., Muck, R.E. and Driehuis, F. 2003. Microbiology of ensiling. Silage science and technology, Proc. Madison: ASCSSA-SSSA. pp. 31-93.
  21. Pan, L., Yang, Z., Wang, J., Wang, P., Ma, X., Zhou, M., Li, J., Gang, N., Feng, G., Zhao, J. and Zhang, X. 2017. Comparative proteomic analyses reveal the proteome response to short-term drought in Italian ryegrass (Lolium multiflorum). PloS one. 12:0184289.
  22. Seo, S. 2005. Forage production and animal husbandry in Korea. Grassland Science. 51:21-25. https://doi.org/10.1111/j.1744-697X.2005.00004.x
  23. Shehzad, M., Ayub, M., Shehzad, M., Akhtar, N., Tahir, M. and Arif, M. 2014. Dry matter yield and forage quality of oat, barley and canola mixture. Pakistan Journal of Agricultural Sciences. 51:443-449.
  24. Succi, G., Crovetto, G.M., Tamburini, A. and Rapetti, L. 1992. Determinazione dell'energianetta di loiessa (Lolium multiflorum) insilataedaffienata. 18:271-283.
  25. Warnke, S.E., Barker, R.E., Jung, G., Sim, S.C., Rouf Mian, M.A., Saha, M.C., Brilman, L.A., Dupal, M.P. and Forster, J.W. 2004. Genetic linkage mapping of an annual x perennial ryegrass population. TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik. 109:294-304. https://doi.org/10.1007/s00122-004-1647-3
  26. Wilkinson, M. and Toivonen, M.I. 2003. World silage. Chalcombe Publications. Painshall, Lincoln, UK.