• Animal Bioscience
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• v.35 no.12
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• pp.1860-1870
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• 2022

Objective: The observation that temperate C₃ and tropical C₄ forage silages easily produce large amounts of ethanol or acetic acid has puzzled researchers for many years. Hence, this study aimed to assess the effects of epiphytic microbiota from C₃ forages (Italian ryegrass and oat) on fermentative products and bacterial community structure in C₄ forage (sorghum) silage. Methods: Through microbiota transplantation and γ-ray irradiation sterilization, the irradiated sorghum was treated: i) sterile distilled water (STSG); ii) epiphytic microbiota from sorghum (SGSG); iii) epiphytic microbiota from Italian ryegrass (SGIR); iv) epiphytic microbiota from oat (SGOT). Results: After 60 days, all the treated groups had high lactic acid (>63.0 g/kg dry matter [DM]) contents and low pH values (<3.70), acetic acid (<14.0 g/kg DM) and ammonia nitrogen (<80.0 g/kg total nitrogen) contents. Notably, SGIR (59.8 g/kg DM) and SGOT (77.6 g/kg DM) had significantly (p<0.05) higher ethanol concentrations than SGSG (14.2 g/kg DM) on day 60. After 60 days, Lactobacillus were predominant genus in three treated groups. Higher proportions of Chishuiella (12.9%) and Chryseobacterium (7.33%) were first found in silages. The ethanol contents had a positive correlation (p<0.05) with the abundances of Chishuiella, Acinetobacter, Stenotrophomonas, Chryseobacterium, and Sphingobacterium. Conclusion: The epiphytic bacteria on raw materials played important roles in influencing the silage fermentation products between temperate C₃ and tropical C₄ forages. The quantity and activity of hetero-fermentative Lactobacillus, Chishuiella, Acinetobacter, Stenotrophomonas, Chryseobacterium, and Sphingobacterium may be the key factors for the higher ethanol contents and DM loss in silages.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.37 no.4
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• pp.372-376
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• 1992

This experiment was conducted to determine hay making time on the drying period, hay moisture content, hay loss content, yield and forage quality of Italian ryegrass in paddy field. Plant height increased rapidly from middle of April, and leaf weight rate were 55.6% at April 20 and 18.9% at May 30. Moisture content and loss content of hay were 17.1-20.5% and 10.2-15.0%, respectively, at drying for 4 days from May 10 to May 30. As the growth stage later, crude protein, fat and ash contents reduced, but crude fiber and NFE contents increased. Dry matter and TDN yield at the cutting of May 20 and May 30 increased to 17-18% and 5-11%, respectively, than those of April 20＋May 30.

• Animal Bioscience
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• v.36 no.8
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• pp.1199-1208
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• 2023

Objective: The purpose of this study was to perform a comparative analysis of metabolite levels in serum and milk obtained from cows fed on different concentrate to forage feed ratios. Methods: Eight lactating Holstein cows were divided into two groups: a high forage ratio diet (HF; 80% Italian ryegrass and 20% concentrate of daily intake of dry matter) group and a high concentrate diet (HC; 20% Italian ryegrass and 80% concentrate) group. Blood was collected from the jugular vein, and milk was sampled using a milking machine. Metabolite levels in serum and milk were estimated using proton nuclear magnetic resonance and subjected to qualitative and quantitative analyses performed using Chenomx 8.4. For statistical analysis, Student's t-test and multivariate analysis were performed using Metaboanalyst 4.0. Results: In the principal component analysis, a clear distinction between the two groups regarding milk metabolites while serum metabolites were shown in similar. In serum, 95 metabolites were identified, and 13 metabolites (include leucine, lactulose, glucose, betaine, etc.) showed significant differences between the two groups. In milk, 122 metabolites were identified, and 20 metabolites (include urea, carnitine, acetate, butyrate, arabinitol, etc.) showed significant differences. Conclusion: Our results show that different concentrate to forage feed ratios impact the metabolite levels in the serum and milk of lactating Holstein cows. A higher number of metabolites in milk, including those associated with milk fat synthesis and the presence of Escherichia coli in the rumen, differed between the two groups compared to that in the serum. The results of this study provide a useful insight into the metabolites associated with different concentrate to forge feed ratios in cows and may aid in the search for potential biomarkers for subacute ruminal acidosis.

• Journal of The Korean Society of Grassland and Forage Science
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• v.43 no.2
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• pp.103-108
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• 2023

Due to the recent impact of global warming, heavy rainfall and droughts have been occurring regardless of the season, affecting the growth of Italian ryegrass (IRG), a winter forage crop. Particularly, delayed sowing due to frequent heavy rainfall or autumn droughts leads to poor growth and reduced winter survival rates. Therefore, techniques to improve yield through additional sowing in spring have been implemented. In this study, the growth of IRG sown in Spring and Autumn was compared and analyzed using vegetation indices during the months of April and May. Spectral data was collected using an Unmanned Aerial Vehicle (UAV) equipped with a hyperspectral sensor, and the following vegetation indices were utilized: Normalized Difference Vegetation Index; NDVI, Normalized Difference Red Edge Index; NDRE (I), Chlorophyll Index, Red Green Ratio Index; RGRI, Enhanced Vegetation Index; EVI and Carotenoid Reflectance Index 1; CRI1. Indices related to chlorophyll concentration exhibited similar trends. RGRI of IRG sown in autumn increased during the experimental period, while IRG sown in spring showed a decreasing trend. The results of RGRI in IRG indicated differences in optical characteristics by sowing seasons compared to the other vegetation indices. Our findings showed that the timing of sowing influences the optical growth characteristics of crops by the results of various vegetation indices presented in this study. Further research, including the development of optimal vegetation indices related to IRG growth, is necessary in the future.

• Journal of The Korean Society of Grassland and Forage Science
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• v.43 no.3
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• pp.190-198
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• 2023

This study was conducted to calculate the damage of Italian ryegrass (IRG) by abnormal climate using machine learning and present the damage through the map. The IRG data collected 1,384. The climate data was collected from the Korea Meteorological Administration Meteorological data open portal.The machine learning model called xDeepFM was used to detect IRG damage. The damage was calculated using climate data from the Automated Synoptic Observing System (95 sites) by machine learning. The calculation of damage was the difference between the Dry matter yield (DMY)_normal and DMY_abnormal. The normal climate was set as the 40-year of climate data according to the year of IRG data (1986~2020). The level of abnormal climate was set as a multiple of the standard deviation applying the World Meteorological Organization (WMO) standard. The DMY_normal was ranged from 5,678 to 15,188 kg/ha. The damage of IRG differed according to region and level of abnormal climate with abnormal temperature, precipitation, and wind speed from -1,380 to 1,176, -3 to 2,465, and -830 to 962 kg/ha, respectively. The maximum damage was 1,176 kg/ha when the abnormal temperature was -2 level (+1.04℃), 2,465 kg/ha when the abnormal precipitation was all level and 962 kg/ha when the abnormal wind speed was -2 level (+1.60 ㎧). The damage calculated through the WMO method was presented as an map using QGIS. There was some blank area because there was no climate data. In order to calculate the damage of blank area, it would be possible to use the automatic weather system (AWS), which provides data from more sites than the automated synoptic observing system (ASOS).

• Journal of The Korean Society of Grassland and Forage Science
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• v.27 no.3
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• pp.183-188
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• 2007

This study was conducted to determine the effects of seeding date on agronomic characteristics, productivity and feed value of Italian ryegrass, barley and rye during cropping after rice in Suwon, middle region of Korea, during 2004 and 2005. In general, early seeding (26 September) showed longer plant length and branch numbers per square meter than late sowing (11 October). Italian ryegrass had more early seeding effect than barley and rye. The yield of each plant was affected by sowing date in all experiments: the earlier seeding had higher yields than late seeding. In case of Italian ryegrass and barley as late-heading varieties showed more early seeding effect than rye. On the other hand, feed value tended to respond differently to yields. The feed value of each plant were based on liquid swine manure (12%) > composted cattle manure (7%) > composted swine manure (2%). The present results highlight the earlier planting obtained higher yield compared to those planted conventionally.

• Journal of The Korean Society of Grassland and Forage Science
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• v.28 no.3
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• pp.215-220
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• 2008

This experiment was conducted to compare forage production and feed value of winter forage crops at the experimental field of Livestock Division, National Institute of Subtropical Agriculture from 2007 to 2008. The experiment was arranged in a randomized block design with three replications. The five winter forage crops used in this experiment were barely ('Youngyang' and 'Yuyeon'), oats ('Samhan' and 'Swan'), wheat ('Kumkang' and 'Woori'), triticale ('Shinyoung') and italian ryegrass ('Hwasan 101' and 'Florida-80'). The species having the fastest heading date was wheat (5th April) while the heading date of oats ('Samhan') was the latest as 28th April. The dry matter (DM) content of wheat was the highest among the five winter forage crops. Italian ryegrass ('Hwasan 101') showed the highest CP content by 11.2% and oats (Swan) showed the lowest content by 9.8%. Average acid detergent fiber (ADF) and neutral detergent fiber (NDF) content were 28.6 and 50.8% respectively. The five winter forage crops have narrow range of total nutrient digestibility (TDN) from 64.0% to 69.1%. Italian ryegrass ('Hwasan 101') showed the highest DM yield by 25,951 kg/ha, but the DM yield of barely and wheat low by 13,892 kg/ha ('Yuyeon') and 14,815 kg/ha ('Woori'), respectively. Italian ryegrass and oats have higher forage yield and feed value in Jeju. Also, oats has high grain and forage yield. The results demonstrated oats can cultivate as alternative winter forage crop for whole crop silage barely in Jeju.

• Journal of Animal Environmental Science
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• v.18 no.3
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• pp.257-266
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• 2012

Near infrared reflectance spectroscopy (NIRS) has become increasingly used as a rapid, accurate method of evaluating some chemical constituents in cereal and dired animal forages. Analysis of forage quality by NIRS usually involves dry grinding samples. Costs might be reduced if samples could be analyzed without drying or grinding. The objective of this study was to investigate effect of sample preparations on prediction ability of chemical composition and fermentation parameter for Italian ryegrass silages by NIRS. A population of 147 Italian ryegrass silages representing a wide range in chemical parameters were used in this investigation. Samples were scanned at 1nm intervals over the wavelength range 680-2500 nm and the optical data recorded as log 1/Reflectance (log 1/R) and scanned in oven-dried grinding and fresh ungrinding condition. The spectral data were regressed against a range of chemical parameters using partial least squares (PLS) multivariate analysis in conjunction with four spectral math treatments to reduced the effect of extraneous noise. The optimum calibrations were selected on the basis of minimizing the standard error of cross validation (SECV) and maximizing the correlation coefficient of cross validation (${R^2}_{CV}$). The results of this study show that NIRS predicted the chemical parameters with high degree of accuracy in oven-dried grinding treatment except for moisture contents. Prediction accuracy of the moisture contents was better for fresh ungrinding treatment (SECV 1.37%, $R^2$ 0.96) than for oven-dried grinding treatments (SECV 4.31%, $R^2$ 0.68). Although the statistical indexes for accuracy of the prediction were the lower in fresh ungrinding treatment, fresh treatment may be acceptable when processing is costly or when some changes in component due to the processing are expected. Results of this experiment showed the possibility of NIRS method to predict the chemical composition and fermentation parameter of Italian ryegrass silages as routine analysis method in feeding value evaluation and for farmer advice.

• Journal of The Korean Society of Grassland and Forage Science
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• v.30 no.3
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• pp.247-256
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• 2010

This experiment was carried out to investigate the effect of seeding rate of newly developed Italian ryegrass varieties, "Kogreen" and "Hwasan 101", on those seed production in Cheonan for three years (2005 to 2007). Two Italian ryegrass varieties were seeded at experimental fields of Grassland and Forage Division, National Institute of Animal Science on late September, and harvested at seed shattering stage. The experiment was arranged in randomized complete block design with three replications by each variety. The treatments was consisted of five seeding rates (5, 10, 20, 30, 40 kg/ha). The average heading date of "Kogreen" was 9 May and "Hwasan 101" was 25 May. The harvesting date of "Kogreen" was on 16 June when the rainy season should not set in. In the case of "Hwasan 101", the harvesting date of seed production was 3 July, because of rainy season. The number of ear per square meter, ear length, spikelet number, seed yield per ha and ripening rate of "Hwasan 101" were 471, 28.5 cm, 24.8, 1,631 kg and 71%, respectively. and those of "Kogreen" were 633, 24.0 cm, 20.8, 2,676 kg and 82.1%, respectively. The shattering property rate of "Hwasan 101" 40.3% was higher than that of "Kogreen" 2.6%. The germination rate of harvested "Hwasan 101" seed was 81.8% and that of "Kogreen" was 79.4%, respectively. In conclusion, the optimum seeding rate of Italian ryegrass for seed production are recommended 20kg/ha in both "Hwasan 101" and "Kogreen".

• Journal of The Korean Society of Grassland and Forage Science
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• v.29 no.4
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• pp.321-328
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• 2009

This experiment was carried out to investigate the effect of cultivar and seeding date on the winter survival rate, quality and DM yield of Italian ryegrass on paddy field for three years in Suwon. Seeding started from 30th Sep. 2003. at intervals of five days and finished 20th Oct. New varieties of Italian ryegrass used in this experiment were "Kospeed", "Kowinmaster" and "Hwasan 101". The winter survival average rate of 5th Oct. seeding plot was 89.8% and it decreased with delayed seeding date. The heading date of "Kospeed" was 7th~13rd May, "Kowinmaster" was 16th May, but "Hwasan 101 ho" didn't show heading until 17th May. Dry matter (DM) yields of 30th Sep. seeding plot were Kospeed 7,909kg/ha, Kowinmaster 6,398 kg/ha and Hwasan 101 ho 5,204 kg/ha. DM yield was decreased with delayed seeding date. Total digestible nutrient (TDN) yield was also decreased with delayed seeding date. Crude protein (CP) content was the highest in Kospeed. seeding plot as 18.3% and in vitro dry matter digestibility (IVDMD) was not showed significant difference among seeding dates.