• 한국균학회지
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• 제16권4호
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• pp.247-252
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• 1988

야생형(野生型)인 만가닥버섯(Lyophyllum ulmarium ASI 8007)의 원형질체(原形質體)로부터 분리(分離)한 염색체(染色體)를 영양요구주(營養要求株)인 잔나비걸상버섯(Ganoderma applanatum ASI 7-18; cys met)의 원형질체내(原形質體內)에 $PEG+CaCl_2$로 섭입(攝入)하였다. 형질전환주(形質轉煥株)는 microtransgenome과 macrotransgenome type으로 전자(前者)는 느리고 불안정성(不安定性)인 균사생장(菌絲生長), 후자(後者)는 아주 빠른 안정성(安定性)인 균사생장(菌絲生長)이 있으며, 균사(菌絲)가 양친(兩親)보다 굵었으며 GCM+benomyl에서 균총분리(菌叢分離)가 일어났다. 이들을 전기영동으로 esterase 동위효소(同位酵素) 패턴을 조사한 결과 양친에 비하여 위치가 다르게 나타났다.

• Environmental Sciences Bulletin of The Korean Environmental Sciences Society
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• 제2권1호
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• pp.37-48
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• 1998

Effect of plant hormones on the leaf senescence of mung bean (Phaseolus radiatus) was investigated by measuring the changes of reducing sugar contents and invertase isozyme activities in detached leaves treated with NAA, $GA_3$ or BA. During dark-induced senescence, reducing sugar contents in the detached leaves increased temporarily at 4 d, thereafter decreased rapidly and reached minimum values within 7-14 d. The pattern of soluble acid invertase activity in the senescing leaves kept in the dark was similar to that of reducing sugar accumulation, whereas the activities of alkaline and extracellular invertases were not significantly changed during leaf senescence. Therefore, these results suggest that soluble acid invertase, but not alkaline and extracellular invertases, induces the accumulation of reducing sugar during leaf senescence of mung bean plants. Exogenous NAA application had little or no effect in the increase of soluble acid invertase activity during dark-induced senescence compared to the control. However, exogenous applications of $GA_3$ and BA led to the increase of soluble acid invertase activity in the senescing leaves. Particularly, BA application was very effective in enhancing the activity of soluble acid invertase as well as in delaying chlorophyll breakdown during dark-induced senescence. These results suggest, therefore, that BA regulates the activity of soluble acid invertase, which leads to the accumulation of reducing sugar, and the stability of photosynthetic apparatus to delay leaf senescence.

• 한국식물병리학회지
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• 제1권3호
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• pp.178-183
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• 1985

수도품종 농백, 진흥, 낙동, 태백 등 4품종과 도열병균 KJ-101과 KJ-301의 2race를 사용하여 peroxidase 활성증가와 저항성과의 관계를 관찰하였다. peroxidase 활성은 수도유균가 성장할수록 모든 품종에서 증가되는 경향은 있었으나 품종간 차이는 크지 않았다. 도열병균을 접종 후 시간이 경과함에 따라 이병 잎에서는 무접종 건전잎보다 효소활성이 높았고 접종된 균주에 비친화적인 품종보다 발병이 심한 친화적 품종에서 더욱 높았다. 전기영동법에 의한 peroxidase pattern은 이병잎에서는 도열병균의 peroxidase band를 관찰할 수 없었다. 질소시비량을 증가시켰을 지라도 peroxidase 활성은 변함이 없었다.

• Animal cells and systems
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• 제4권1호
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• pp.23-30
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• 2000

Horizontal starch gel electrophoresis for 29 populations (n=543) of the wrinkled frog, Rana rugosa, from Korea and Japan was peformed to assess the degree of genic variation and genetic diversity, and to understand the biogeographic pattern of distribution and speciation. A sum of 22 presumptive loci was screened from 17 enzymes and general proteins. Four loci, Aco, Est-3, Me-2, and Pgm, demonstrated high levels of polymorphism. The degree of average genetic variation of R. rugosa was P=22.7% (9.1-40.9%), Ho=0.086 (0.048-0.165) and He=0.090 (0.042-0.168). In the south-eastern region of the Korean peninsula (Chongsong, Yongchon, Ulsan, Kyongju, Pohang, yongdok and Ulchin), a few unique alleles in the Mpi locus were detected and their biogeographic implications were considered. The degree of genetic differentiation among the Korean populations was moderate (S=0.900), whereas the degree of genetic diversity between Korean and Japanese populations was notably high (S=0.687, D=0.293). This result corresponds with the data obtained by the mitochondrial cytochrome b gene sequence (Lee et al., 1999) suggesting that the Korean and Japanese R. rugosa might have evolved a specific level of genetic differentiation since their geographic isolation.

• 한국식물학회:학술대회논문집
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• 한국식물학회 1987년도 식물생명공학 심포지움 논문집 Proceedings of Symposia on Plant Biotechnology
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• pp.157-167
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• 1987

The have been many reports that phenoloxidase are correlated with development in many fungi. C. congregatus, one of nushroom-forming basidiomycetes, which requires light for its development also has phenoloxidases. In C. congragatus, there are two sets of membrane-associated phenoloxidase (PHO I and PHO II) which are differentiated by their isozyme patterns, and each enzyme set consists of two different subtrate specific enzyme protein; o-tolidine reacting enzyme, and DOPA reacting enzyme. PHO I which is localized by a protoplast-concanavalin A technique by using a new solidifying agent, Pluronic Polyol F 127, instead of agar appears in the vegetative hyphae, and PHO II appears at the early primordial stage on agar and at the sclerotial stage of liquid shake cultures. Inhibition of PHO I with the enzyme inhibitors inhibits mushroom formation as well as melanization of the vegetative hyphae at concentrations which do not inhibit the vegetative growth. PHO I deficient mutants do not form mushrooms or melanins, and the mutants show abnormal nuclear migration patterns. PHO II has roles; possibly cementing the adjacent hyphae during the actual three dimensonal structure formation, and melanizing mushrooms and sclerotia. The possible roles of PHO I in the light reception complex and in melanin formation, the function of malanin, and possible roles of postulated post translational modifying enzymes which regulate the phenoloxidases, nuclear migration pattern, and self-nonself recognition mechanism are discussed.

• 한국환경과학회지
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• 제2권1호
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• pp.37-48
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• 1993

Effect of plant hormones on the leaf senescence of mung bean (Phseoln radiatus) was investigated by measuring the changes of reducing sugar contents and invertase isozyme activities in detached leaves treated with NAA, $GA_3$ or BA. During dark-induced senescence, reducing sugar contents in the detached leaves increased temporarily at 4 6, thereafter decreased rapidly and reached minimum values within 7-14 6. The pattern of soluble acid invertase activity in the senescing leaves kept in the dark was similar to that of reducing sugar accumulation, whereas the activities of alkaline and extracellular invertases were not significantly changed during leaf senescence. Therefore, these results suggest that soluble acid invertase, but not alkaline and extracellular invertases, induces the accumulation of reducing sugar during leaf senescence of Rung bean plants. Exogenous NAA application had little or no effect In the increase of soluble acid invertase activity during dark-induced senescence compared to the control. However, exogenous applications of $GA_3$ and BA led to the increase of soluble acid invertase activity in the senescing leaves. Particularly, BA application was very effective In enhancing the activity of soluble acid invertase as well as in delaying chlorophyll breakdown during dark-induced senescence. These results suggest, therefore, that BA regulates the activity of soluble acid invertase, which leads to the accumulation of reducing sugar, and the stability of photosynthetic apparatus to delay leaf senescence.

• Parasites, Hosts and Diseases
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• 제59권1호
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• pp.1-8
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• 2021

Leptotrombidium scutellare is one of the 6 main vectors of scrub typhus in China. It has been found in more than 15 provinces of China. Especially in Yunnan, it was found to be mainly distributed in some mountainous areas with high altitude, low temperature and low precipitation. Rodents and some other small mammals were the most common hosts of L. scutellare. To date, more than 40 host species of L. scutellare have been recorded with very low host specificity, and the main hosts varied in different geographical regions. L. scutellare had a strong resistance against the cold environment, and the temperature and humidity were 2 important factors affecting its growth and development. Among different individuals of their rodent hosts, L. scutellare mites often showed an aggregated distribution pattern, which reflected the interspecific cooperation of the mites. The chromosome karyotype of L. scutellare was 2n=16 and all the 8 pairs of chromosomes were short rod-shaped with metacentric or sub-metacentric types. The isozyme spectrum supported that L. scutellare, L. deliense and L. rubellum were in the same species group. Based on the natural infection, experimental transmission and epidemiological evidence, L. scutellare has been eventually confirmed as the second major vector of scrub typhus in China, which is second only to L. deliense.

• 한국균학회지
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• 제15권4호
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• pp.250-253
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• 1987

원형질체내에 외래유전물질 도입에 의한 균주 개발의 가능성 검토의 일환으로 사철느타리의 원형질체로부터 핵을 분리하여 느타리의 원형질체내로 전이하여 얻은 결과는 다음과 같았다. 1. 핵전이주는 크게 3가지 type으로 구분되었는데 type 1은 아주 빠른 균사생장과 clamp connection를 형성하지 않으며 CM+benomyl에서 균총분리가 나타남으로서 이질이배체로 사료되었다. type 2는 주된 핵전이주로서 모균주와 비슷하거나 다소 빠른 균사생장과 clamp connection을 지녀 자실체를 형성하였으며 heterokaryon으로서 원형질체 융합주와 유사하였다. 그러고 type 3은 핵 또는 염색체 단편의 전이로 clamp connection를 지니지 않고 아주 느리거나 MM에서 생육이 거의 불가능한 균주였다. 2. 핵전이주 및 모균주를 전기영동법으로 esterase 동위효소 pattern을 조사한 결과 핵전이주는 모균주와 구별되었는데 type 1인 이질이배체는 새로운 band를 나타내었으며, type 2와 type 3은 모균주들을 합한 것으로 보였다.

• 한국약용작물학회지
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• 제4권1호
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• pp.52-57
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• 1996

국내에 재배되는 마(Dioscorea batatas) 주요품종으로는 식용으로 널리 쓰이는 단마, 약용으로 이용되는 장마 그리고 최근 식용마로 도입육성된 축근종(筑根種) 등이 있다. 본 연구는 이들 세종류의 마 주요품종을 대상으로 동위효소 분석과 생육특성 및 수량성을 비교하여 얻어진 결과이다. 1. 마 주요품종의 동위효소 분석 마 주요품종의 동위효소를 분석한 결과 단백질의 band pattern은 잎, 엽병, 줄기 모두에서 Band의 수와 위치의 차이를 보였다. Peroxidase의 동위효소는 잎과 엽병은 거의 동일하게 조사되었으나 줄기조직에 의해 구분이 가능하였다. 그리고 Esterase의 경우는 잎과 엽병, 줄기 모두에서 band pattern의 차이를 보여 품종간 구분이 가능하였다. 2. 마 주요품종의 생육특성 및 수량비교 엽형비교에서 단마와 장마는 상위엽이 피침형이고 축근종(筑根種)은 장심장형 이었으며, 엽서는 장마와 단마의 상위엽이 호. 대생을 보인반면 축근종(筑根種)은 대생이었다. 뿌리 생육과 수량성 비교에서 괴 근수가 단마 1.2개, 장마 1.3개지만 축근종(筑根種)은 1개였다. 괴근장은 단마와 장마가 각각 30cm와 51cm로 길었지만 축근종(筑根種)은 9.7cm로 짧았다. 괴근수량은 단마가 2,751kg/10a로 가장 높았고 장마가 2,288kg/10a였으며, 축근종(筑根種)은 1,785kg/10a였다. 축근종(筑根種)의 수량은 단마와 장마에 비하여 적었으나 단괴형이어서 기계화 수확에 유리한 품종으로 생각되었다.

• 대한의생명과학회지
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• 제5권1호
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• pp.119-129
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• 1999

호르텐스극구흡충 성충 (adult worm)을 재료로 하여 acid phosphatase (Acp)와 alkaline phosphatase (Alp)의 조직 세포에 대한 분포 및 동위효소의 특성을 규명하기 위하여 Gomori lead nitrate 및 Gomori calcium방법 그리고 전기영동법 등을 시행하였다. 조직 내의 Acp및 Alp의 분포는 충체의 소장 (intestine)과 난황선 (vitellaria)그리고 인두 (pharynx)에서 강한 반응을 나타냈다. 동위효소 유형은 Acp에서 179.5 및 209.4 kDa 두 개의 분획이 분리되었고, Alp는 145.9, 207.5 및 220.8 kDa의 세 개의 분획이 분리되었다. 열에 대한 안정도는 Alp가 9$0^{\circ}C$에서 12초경과 후에 완전히 불활성화 되었으며, Alp의 최적 온도 및 pH는 4$0^{\circ}C$와 pH 9이었고 Acp의 치적 pH는 5였다. 그리고 Acp및 Alp의 최대 활성도 (unit)는 Acp가 71 범위였고, Alp는 189의 범위에 있었다. 이상에서와 같이 호르텐스극구흡충은 phosphatase가 소화관과 난황선에서 주로 분포하고 있었으며 숙주 체내에서 소화관을 중심으로 대사가 활발하게 이루어지고 있음을 알 수 있었다.