The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Uptake of Isolated Lyophyllum ulmarium Chromosomes by Ganoderma applanatum Protoplasts

잔나비걸상버섯 원형질체(原形質體)에 의(依)한 만가닥버섯 염색체(染色體)의 섭입(攝入)

  • Yoo, Young-Bok (Applied Mycology and Mushroom Division, Agricultural Science Institute RDA) ;
  • You, Chang-Hyun (Applied Mycology and Mushroom Division, Agricultural Science Institute RDA) ;
  • Chang, Kwon-Yawl (Department of Agronomy, College of Agriculture Gyeongsang National University)
  • 유영복 (농촌진흥청 농업기술연구소 균이과) ;
  • 유창현 (농촌진흥청 농업기술연구소 균이과) ;
  • 장권열 (경상대학교 농과대학 농학과)
  • Published : 1988.12.30
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Abstract

The uptake of isolated Lyophyllum ulmarium chromosomes by Ganoderma applanatum protoplasts was induced with polyethylen $glycol+CaCl_2+glycine$. Uptake products of chromosomes by protoplasts showed micro-and macrotransgenome type. The former was slowly growing and unstable, the latter was outgrowing and stable mycelial colonies which made thick hyphae of width and segregation of mycelial colony on GCM containing benomyl. A comparison of macrotransgenome type was using isozyme analysis of esterase. The enzyme pattern of two transformants was distinct in position and quantity compared with parents.

야생형(野生型)인 만가닥버섯(Lyophyllum ulmarium ASI 8007)의 원형질체(原形質體)로부터 분리(分離)한 염색체(染色體)를 영양요구주(營養要求株)인 잔나비걸상버섯(Ganoderma applanatum ASI 7-18; cys met)의 원형질체내(原形質體內)에 $PEG+CaCl_2$로 섭입(攝入)하였다. 형질전환주(形質轉煥株)는 microtransgenome과 macrotransgenome type으로 전자(前者)는 느리고 불안정성(不安定性)인 균사생장(菌絲生長), 후자(後者)는 아주 빠른 안정성(安定性)인 균사생장(菌絲生長)이 있으며, 균사(菌絲)가 양친(兩親)보다 굵었으며 GCM+benomyl에서 균총분리(菌叢分離)가 일어났다. 이들을 전기영동으로 esterase 동위효소(同位酵素) 패턴을 조사한 결과 양친에 비하여 위치가 다르게 나타났다.

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