• Applied Science and Convergence Technology
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• v.24 no.5
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• pp.132-135
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• 2015

Schematic of RAON vacuum system is introduced. Vacuum test for superconducting cavity with liquid nitrogen is performed. Schematic plan for RAON vacuum system is introduced and vacuum control system for superconducting cavity test is constructed. Vacuum pressure of cavity is shown as a function of pumping time. The temperature of cavity is shown as a function of cooling time. Outgassing species from cavity is also detected. Detailed experimental procedure is presented to test the cavity vacuum with liquid nitrogen.

• Journal of the Korean Magnetic Resonance Society
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• v.24 no.3
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• pp.77-85
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• 2020

Stable isotope enrichment in proteins is necessary for high-resolution nuclear magnetic resonance (NMR) experiments. Although methods for ¹³C, ¹⁵N and ²H-enrichment in prokaryotic cells are well established, full processing and correct folding of complex protein systems require higher organisms as the expression host. In the present study, we review recent efforts to enrich stable isotopes in mammalian cells for protein NMR studies.

• 대한핵의학회:학술대회논문집
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• 1970.10a
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• pp.94.1-94.1
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• 1970

• Proceedings of the KSEEG Conference
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• 2001.04a
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• pp.212-213
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• 2001

• Analytical Science and Technology
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• v.5 no.2
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• pp.229-234
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• 1992

Stable Isotope Mass Spectrometric technique was studied for quality assessment of Korean Honey and compared with the current regulation for the feasibility of adaptation to the regulation.

• 한국문화재보존과학회:학술대회논문집
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• 2005.11a
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• pp.268-271
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• 2005

Sixty-four obsidian artifacts found in the Korean Peninsula have been characterised by a study of their minor elements and Sr isotope ratio. The artifacts are from the following locations: Yondaedo Island; Yokjido Island; Sangnodaedo Island, Tongyeong, Gyeongnam; Dongsamdong, Busan; Songdo Island, Yeosu, Jeonnam; Suyanggae, Danyang, Chungbuk; Sangmuyongri, Yanggu, Gangwondo; Paektusan Mountain; Kyushu region, Japan. The study of minor elements and Sr isotope ratio recognised three distinct major groups. This result suggests that there are no correlations among the three groups whose provenances are different. But as a result of classification using Sr isotope ratio, obsidian artifacts found in the southern part of the Korean Peninsula classified two groups within C group. This research will furnish scientific datum to be able to mutual comparison of obsidians excavated from other site.

• Applied Science and Convergence Technology
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• v.24 no.4
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• pp.77-83
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• 2015

Many welding processes are performed to construct cryogenic system. Leak-tight for the cryogenic system is required at low temperature environment. Helium leak detection techniques are commonly used to find leak for the cryogenic system. The helium leak detection techniques for spraying, sniffing and pressurizing techniques are introduced. High vacuum is also necessary to use helium leak detector. So, types of fluid flow, effective temperature, conductance and pumping speed are introduced for vacuum pumping. Leak test procedure is shown for pipe welding, cryomodule and low temperature test. Cryogenic seals which include copper gasket, helicoflex gasket and indium are investigated.

• Bulletin of the Korean Chemical Society
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• v.23 no.11
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• pp.1541-1544
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• 2002

The concentration of B in steels is important due to its influence on mechanical properties of steel such as hardenability, hot workability, and creep resistance. An analytical method has been developed to determine B in steel samples by high-resolution inductively coupled plasma mass spectrometry (HR-ICP-MS). National Institute of Standard and Technology Standard Reference Material (NIST SRM) 348a was analyzed to validate the analytical method. The steel sample was digested in a centrifuge bottle with addition of aqua regia and $^{10}B$ spike isotope. Sample pH was then adjusted to higher than 10 to precipitate most matrix elements such as Fe, Cr, and Ni. After centrifugation, the supernatant solution was passed through a cation exchange column to enhance the matrix separation efficiency. B recovery efficiency was about 37%, while matrix removal efficiency was higher than 99.9% for major matrix elements. The isotope dilution method was used for quantification and the determined B concentration was in good agreement with the certified value.

• Journal of the Korean Magnetic Resonance Society
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• v.18 no.2
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• pp.47-51
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• 2014

Larger proteins (above molecular weight 50 kDa) usually show slow motional tumbling in solution, which facilitates the decay of NMR signal, resulting in poor signal-to-noise. In the past twenty years, researchers have tried to overcome this problem with higher molecular weight by improvement of hardware (higher magnetic field and cryoprobe), optimization of pulse sequences for lager molecules, and development of isotope-labeling techniques. Actually, GroEL/ES complex (${\approx}$ 900 kDa) was successfully studied using combination of above techniques. Among the techniques used in large molecular studies, the impact of isotope-labeling for large molecules study is summarized and discussed here.

• Journal of Microbiology and Biotechnology
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• v.16 no.7
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• pp.1139-1143
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• 2006

Various techniques and strategies have been developed for the identification of intracellular metabolic conditions, and among them, isotope balance-based flux analysis with gas chromatography/mass spectrometry (GC/ MS) has recently become popular. Even though isotope balance-based flux analysis allows a more accurate estimation of intracellular fluxes, its application has been restricted to relatively small metabolic systems because of the limited number of measurable metabolites. In this paper, a strategy for incorporating isotope balance-based flux data obtained for a small network into metabolic flux analysis was examined as a feasible alternative allowing more accurate quantification of intracellular flux distribution in a large metabolic system. To impose GC/MS based data into a large metabolic network and obtain optimum flux distribution profile, data reconciliation procedure was applied. As a result, metabolic flux values of 308 intracellular reactions could be estimated from 29 GC/ MS based fluxes with higher accuracy.