• Title/Summary/Keyword: Isolation unit

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Analysis of cellular fatty acid methyl esters (FAMEs) for the identification of leuconostoc strains isolated from kimchi

  • Lee, Jung-Sook;Chun, Chang-Ouk;Kim, Hong-Joong;Joo, Yun-Jung;Lee, Hun-Joo;Park, Chan-Sum;Park, Yong-Ha;Mheen, Tae-Ick
    • Journal of Microbiology
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    • v.34 no.3
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    • pp.225-228
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    • 1996
  • The cellular fatty acid methyl esters (FAMEs) analysis data obtained for clusters defined at a Euclidian distance of 17.5, in the classification of lactic acid bacteria isolated from kimchi, described by Lee et al. (4), was used for the identification of 79 Leuconostoc isolates. The test strains were isolated using a selective isolation medium specific for the genus Leuconostoc. These strains were then characterized according to their fatty acid profiles. The results show that all seventy nine test strains were identified to the known Leuconostoc clusters B, C, and D. Cluster B had the highest relative amount of the saturated fatty acid 16 : 0. The saturated fatty acid 16 : 0 and summed feature 9 were found as a major components in cluster C, which had a higher level of summed feature 9 than cluster B. Cluster D is characterized by the highest relative amount of the unsaturated fatty acid 18 : 1 w9c. It is suggested that FAMEs analysis can be successfully applied in the identification of lactic acid bacteria isolated from kimchi.

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Isolation and Identification of ${\gamma}$-Cyclodextrin Glycosyltransferase-Producing Bacteria, and Its Production Conditions (${\gamma}-Cyclodextrin$ Glycosyltransferase 생산균주의 분리, 동정 및 효소 생산조건)

  • Kim, Myung-Hee;Lim, Young-Hee;Bae, Kyung-Sook;Oh, Tae-Kwang;Sohn, Cheon-Bae
    • Applied Biological Chemistry
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    • v.40 no.6
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    • pp.459-464
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    • 1997
  • A cyclodextrin glycosyltransferase-producing bacterium was newly isolated from soil using alkaline pH medium containing 1% $Na_2CO_3$. The isolated strain was identified as Bacillus brevis by morphological and biochemical characteristics, and fatty acid composition and designated Bacillus brevis CD162. The strain showed the best enzyme production of 0.9 unit/ml after 96 hrs of culture at $30^{\circ}C$ in a medium of 2.0% soluble starch, 0.75% yeast extract, 0.5% bacto peptone, 0.2% $K_2HPO_4$ 0.05% $MgSO_4{\cdot}7H_2O$, and 1.5% $Na_2CO_3$ at initial pH 10.2.

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Interference Probability Calculation of Frequency Sharing Between Non-Geostationary-Satellite Orbit Mobile Earth Station and Land Mobile Station (비정지궤도 위성이동지구국과 육상이동간의 주파수 공유에 따른 간섭 확률 계산)

  • 박지웅;최재훈;김희동
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.23 no.7
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    • pp.1807-1816
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    • 1998
  • The interference probability between non-geostationary-statellite orbit mobile station is calculated when the two systems are sharing the frquency band below 1 GHz. The probability density function of a mobile earth station(MES) is calculated based on the established propagation model and then, a probability of exceeding the threshold level is derived. By changing the average transimission per unit time of total MSS(Moblie satellite service) area and the ratio of transmitters for specific area, we obtain the average transmission per unit time for the area under consideration. From this, the exceedance probability for the given pfd threshold level is evaluated. The exceedance probability is increased as the average transmissio per unit time and ratio become larger. Also the effect of filter isolation between channels is accounted for.

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Characteristics of Traditional Wine-Koji and Isolation of Fungi (전통주 제조용 발효제의 특성 및 진균류의 분리)

  • Jeong, Seung-Chan;Yu, Mi-Jung;Cho, Yun-Kyoung;Lee, Jong-Soo
    • The Journal of Natural Sciences
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    • v.13 no.1
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    • pp.73-82
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    • 2003
  • Microflora and enzyme activity of traditional wine-koji were investigated. Bacteria was contained the greatest of $1.3\times10^7$ CFU/g-Koji, and its amylase and protease activities were 120.0 u/g and 36.6 u/g, respectively. 6 Kinds of yeast were isolated from the koji and identified as Hansenula alni (No 1), Hansenula canadensis (No 2), Hansenula silvicola (No.3), Hansenula califrnica (No 4), Hansenula beijerinckii (No 9) and Hansenula saturnus var. sturnus (No11). Furthermore, 14 kinds of mold were also isolated from the koji and identified as Rhizopus sp(No 1-41, 11 species) and Aspergillus sp.(No. 46, 53, 64, 3 species). Only Aspergillus sp. No 46 was showed a-amylase activity of 5.5 Unit and protease activity of Rhizopus sp. No 8 was the highest of 45.0 Unit.

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Isolation of a Bacterium That Inhibits the Growth of Anabaena cylindrica

  • Kim, Chul-Ho;Leem, Mi-Hyea;Choi, Yong-Keel
    • Journal of Microbiology
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    • v.35 no.4
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    • pp.284-289
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    • 1997
  • A Gram (-), rod-shaped bacterium $2.3{\sim}2.8{\times}0.45{\mu}m$ in size which exhibited growth-inhibiting effects against a cyanobacterium (Anabaena cylindrica) was isolated from Daechung Dam Reservoir. This isolate was identified as Moraxella sp. and designated Moracella sp. CK-1. Hollow zones formed around bacterial colonies on the cyanobacterial lawn. In a mixed-culture of A. cylindrica and the isolate, each microorganism grew inverse-proportionally, and the cyanobacterial vegetative cells completely disappeared within 24 hours. On treatment with Moraxella sp. CK-1, cell walls of A. cylindrica disappeared, but sheathes remained in a more electron dense form. The unit membrane such as thylakoidal membrane was stable to bacterial lysing activity. This bacterium showed a broad action spectrum against cyanobacteria. The growth-inhibiting activity of Moracella sp. CK-1 against A. cylindrica is believed to be performed through the excretion of active substances.

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A Study on the Architectural Planning of the Ward in Mental Hospital (II) -focused on the Composition of the Nurse Unit- (우리 나라 정신병원 병동부에 관한 건축계획적 연구 (II) -간호유닛의 구성을 중심으로-)

  • Choi, Sung-Bong;Choi, Young-Jib;Kim, Kwang-Moon
    • Journal of The Korea Institute of Healthcare Architecture
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    • v.4 no.7
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    • pp.9-18
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    • 1998
  • Even though the interest to the mental hospital is recently increased, the mental hospitals are not well developed due to the pending arrangement of the concept of returning to the society and the system of local communities. Also, the hospitalism due to the isolation of patients and the long term stay in hospitals has become an issue. Despite the present circumstances, the prototype of mental health care in many field are investigated and progressively fixed. This study attempts to examine the current status of domestic and foreign mental institution and to establish the design guideline of the wards of the mental hospitals.

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Design of Broad Band RF Components for Partial Discharge Monitoring System (부분방전 모니터링 시스템을 위한 광대역 RF 소자설계 연구)

  • Lee, Je-Kwang;Ko, Jae-Hyeong;Kim, Koon-Tae;Kim, Hyeong-Seok
    • The Transactions of The Korean Institute of Electrical Engineers
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    • v.60 no.12
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    • pp.2286-2292
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    • 2011
  • In this paper we present the design of Low Noise Amplifier(LNA), mixer and filter for RF front-end part of partial discharge monitoring system. The monitoring system of partial discharge in high voltage power machinery is used to prevent many kinds of industrial accidents, and is usually composed of three parts - sensor, RF front-end and digital microcontroller unit. In our study, LNA, mixer and filter are key components of the RF front-end. The LNA consists of common gate and common source-cascaded structure and uses the resistive feedback for broad band matching. A coupled line structure is utilized to implement the filter, of which size is reduced by the meander structure. The mixer is designed using dual gate structure for high isolation between RF and local oscillator signal.

Unscented Kalman Filter For Aircraft Sensor Fault Detection

  • Kim, In-Jung;Kim, You-Dan
    • 제어로봇시스템학회:학술대회논문집
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    • 2003.10a
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    • pp.2335-2339
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    • 2003
  • To prevent the critical situation due to the fault in the aircraft sensor system, the fault tolerant system with triple or quadruple redundancy can be made. However, if the faults are occurred in two or more than sensors simultaneously, the conventional fault detection process, such as cross-channel monitoring, may give the wrong fault alarm. For this case, we can detect the fault by estimating the state vector based on the system dynamics model, which is nonlinear for aircraft. In this paper, we propose the unscented Kalman filter to estimate the nonlinear state vector. This filter utilizes the so-called unscented transformation of sigma points featured the statistical characteristics of the random variable. For verification, we perform the simulations for F-16 aircraft with accelerometers, gyros, GPS and air data system.

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Analysis of the Reduction of Light-weight Imapct Noise for Load Condition of Floating Floor (뜬바닥층의 하중조건에 따른 경량충격음 저감량 분석)

  • Kim, Myung-Jun;Lee, Sung-Ho;Yang, Jae-Hoon
    • Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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    • 2006.11a
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    • pp.356-360
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    • 2006
  • Recently, for the purpose of improving the isolation performance of impact noise, many resilient materials have been installed in a residential building. As one of the reduction method for improving the performance of light-weight impact noise, this study is focused on the load condition of floating layer over resilient material. We studied the correlation between the mass or load of the floating layer and the reduction of light-weight impact noise by experiments in reverberation chamber for testing the impact noise. The results show that the reduction of impact noise is improved by increasing the mass per unit area of floating layer until about $140kg/m^2$. But the reduction is not obvious by adding extra mass on the floating layer.

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A Study on the Architectural Planning of a Maternity Ward Focused on Rooming-in System (모자동실 개념을 중심으로 한 산과 병동의 건축계획에 관한 연구)

  • Kim, Eon-Hwa;Yu, Young-Min
    • Journal of The Korea Institute of Healthcare Architecture
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    • v.8 no.2
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    • pp.25-33
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    • 2002
  • The effects and necessity of the breast milk are well known, but the breast-feeding rate in our country is very low. One of the reason is due to the separation policy of mother and baby after delivery. The mother is in the inpatient-room and the baby is in the new-born baby room. This isolation operation of hospital get increased according to the decreasing ratio of breast feeding in Korea. Only a few hospital operates and provides the space for a new-born baby in the mother's room. The system of baby and mother in one room is adopted and operated due to the breast feeding campaign. It is very encouraging, but the space for the baby and the mother is not enough in multi-bed rooms. We should reconsider the relationship between the new-born baby unit and the obstetric ward, and design the patient's room for the baby and the mother.

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