• Journal of Embryo Transfer
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• v.25 no.2
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• pp.93-96
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• 2010

The isolation of preantral follicles from the ovaries of bovine was performed under mechanical and enzymatic methods. A significant increase in the total number of follicles retrieved was detected when tissue chopper was used. Micro-dissection could supply good quality, larger sized follicles (400 to $700{\mu}m$) but with the lowest yield ($9.0{\pm}1.0$). The isolated preantral and early antral follicles were cultured for 14 days. Follicles isolated by the mechanical method had a greater growth during a culture period than follicles collected enzymatically. Morphologically normal bovine oocytes from early antral follicles after 14 days culture were 59.6% after culture and after 24 h of maturation culture, 12.9% of in vitro-grown oocytes reached the second metaphase. In conclusion, this study showed that mechanical method can be used effectively to isolate intact preantral follicles from bovine ovaries.

• The Korean Journal of Physiology and Pharmacology
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• v.13 no.5
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• pp.357-360
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• 2009

It is hypothesized that a number of environmental factors affect animals' behavior. Without controlling these variables, it is very hard for researchers to get not only reliable, but replicable data from various behavioral experiments testing animals' cognitive as well as emotional functions. For example, laboratory mice which had restricted environment showed different synaptic potentiation properties with wild mice (Zhao MG et al., 2009). While performing behavioral experiments, however, it is sometimes inevitable that the researcher changes the animals' environments, as by switching the cages in which experimental animals are housed and separating animals raised together into small experimental groups. In this study, we investigated the effect of environmental changes on mice's emotional behaviors by socially isolating them or reducing the size of their cage. We found that social isolation selectively increases the animals' levels of anxiety, while leaving depression-like behaviors unchanged. On the other hand, alteration of the housing dimensions affected neither their anxiety levels nor their depression-like behaviors. These results suggest that environmental variables may have a prominent impact on experimental animals' emotional behaviors and possibly their psychological states, leading to bias in the behavioral data produced from experiments.

• Korean Journal of Pharmacognosy
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• v.34 no.3 s.134
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• pp.206-209
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• 2003

The fruit of Evodia officinalis (Rutaceae) is known for containing a number of indoloquinazoline and quinoline type alkaloids. Evodiamine, evocarpine and rutaecarpine are the major constituents of Evodiae Fructus. These alkaloids were isolated and determined by forming complex compounds from Evodiea Fructus. For the determination of these alkaloids, a new spectrophotometric method was developed with a simple and selective sample clean-up using thiocyanatocobaltate [II] complex ion. The absorbance of alkaloidal complex in 1.2-dichloroethane solution was measured at 625 nm. A calibration curve for the alkaloids isolated from Evodia Fruit was linear over the concentration range of $1.0{\sim}6.0\;mg/ml$. The method proved to be rapid, simple and reliable for the isolation and the determination of the alkaloids in Evodiae Fructus.

• Journal of Veterinary Clinics
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• v.22 no.3
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• pp.244-248
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• 2005

This study was conducted to survey the relationship between the somatic cell count (SCC), and California mastitis test (CMT) & mastitis. A total of 328 quarter milk samples were collected from 211 cows suspected to have mastitis; Both SCC and CMT were performed on the samples. Milk smear was stained with Broadhurst and Paley stain and the cells were classified into either epithelial or blood cells. Bacterial isolation was made and antimicrobial susceptibility was tested. Of the 328 quarters, 78 ($23.8{\%}$) were CMT negative with SCC <750,000/ml. As expected, CMT score increased with the increase of SCC. The number of epithelial cells decreased with the increasing number of somatic cells, while the opposite was the case with the number of blood cells. The critical point was when the SCC reached 1,000,000/ml. Up to 1,000,000 cells/ml, the number of epithelial cells was greater than that of blood cells. The results indicate that when epithelia:blood cell ratio is 58.1:41.9, the milking line should be checked and bacterial isolation be performed on the samples in order to identity mastitis.

• Journal of the Earthquake Engineering Society of Korea
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• v.12 no.1
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• pp.67-77
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• 2008

Seismic isolation has been studied continuously as a solution of the seismic engineering to reduce the sectional forces and the damages of structures caused by earthquakes. To certify reliable design and installation of the seismic isolation systems, seismic isolation bearings should be fabricated under well planned quality control process, and proper evaluation tests for their seismic performance should be followed. In this study, shear property evaluation tests for the lead rubber bearings(LRB) and the rubber bearings(RB) were implemented and the temperature dependency tests were also implemented to evaluate the changes of shear properties according to the changes of temperature. After evaluation tests, the measured shear properties were compared to their design values and their deviation was analyzed comparing with the allowable error ranges specified in Highway Bridge Design Specifications. These results showed that a considerable number of isolation bearings have so large deviations from their design values that their error ranges were over or very close to the allowable ranges. And the test results for temperature dependency showed that the shear properties of isolation bearings would be changed in great degree by the change of temperature during their service period. If these two types of changes in their shear properties are superposed, it would possible that the changes of shear properties from their original design values are over than 50%.

• Korean Journal of Poultry Science
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• v.23 no.2
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• pp.77-84
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• 1996

The purpose of this paper is to introduce the present situation of broiler breeder industry, and to investigate some problems related to it in Korea. The number of broiler breeding farms were 231 in 1990. It peaked to 264 in 1992, but decreased afterward to 232 in 1995. The number of broiler parent stock were over 4 millions since 1992 except in 1993. The number of commercial chicks produced per breeding hen was around 100, which is far below to the suggested values of breeding companies. The number of hatching eggs produced per breeding hen were 168 eggs in US, 156~170 eggs in Thailand, and 160~170 eggs in China. Factors involved in the lowered performances of broiler parent stock in Korea appear to be 1) the poor isolation of breeding farms from commercial broiler farms, causing easy contamination with various diseases, 2) the failure to provide proper environment to the breeder, and 3) the failure to control body weight during laying periods.

• Journal of Plant Biotechnology
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• v.34 no.4
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• pp.363-373
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• 2007

To establish an efficient and reliable microspore culture system for pepper (Capsicum annuum L.), the effect of light intensity used for donor plant's growth, microspore isolation methods, the composition of culture medium, and culture period in light on the production of embryos were investigated. The viability of microspores taken from the plants grown under the light intensity of 10,000 lux was almost same as that from the lower (5,500 lux) light intensity, and the embryo induction and development were a bit higher when donor plants were grown under the lower light intensity. This result implies that lower light intensity does not interfere with the embryo induction and development. However, it was very difficult to prepare microspores for culture since only a small number of flower buds could be harvested from plants grown under the light intensity of 5,500 lux. Microspore isolation methods greatly affected microspores viability; that is, when microspores were isolated by blending rather than maceration, the greater number of viable microspores were easily generated (about 13 times). Among media used for microspores culture in this study, MN medium was most efficient for embryo induction and development. Total number of embryos and the number of cotyledonary embryos were highest when microspores were cultured in dark for 4 weeks, and then in light for one week. These results will be provide valuable information to set up efficient microspore culture system of hot pepper with a high frequency of embryo production, which are applicable to gene transformation and mutagenesis.

• Korean Journal of Veterinary Research
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• v.28 no.1
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• pp.59-65
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• 1988

This paper deals with the 0 groups of citrate utilizing variants of Escherichia coli ($Cit^+$ E. coli) isolated from cattle, the production of colicin, hemolysin, K99 antigen, heat stable enterotoxin, and the isolation of plasmid DNA. Among 42 $Cit^+$ E. coli, 12 strains were 020, 9 strains 08, 5 strains 045, 3 strains 0115, 1 strain 064, 1 strain 0139 and remaining strains(11) were untypable. Thirty-nine(81.3%) out of 48 $Cit^+$ E. coli were produced colicin and 13(27.0%) were produced hemolysin. Of 12 $Cit^+$ E. coli bearing K99 antigen, 6(50.0%) were produced heat stable enterotoxin. In gel electrophoresis for the isolation of plasmid DNA, the number of plasmids varied from 1 to 7 in 10 $Cit^+$ E. coli. It's molecular weight ranged from 2 to 50 Mdalton, and 50 Mdalton plasmid was commonly existed in all strains.

• 제어로봇시스템학회:학술대회논문집
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• 2005.06a
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• pp.1239-1243
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• 2005

A multipath mitigation method using the fault detection and isolation technique is proposed for the CDGPS. The base station is assumed to be immune to the effect of the multipath. With this reasonable assumption, the effect of multipath in moving station is mitigated. For that, the double difference measurement is produced, and then another additional difference between code pseudorange and acclumulated carrier phase is calculated. The test statistic is constituted with those differences. The hypothesis testing is applied to that test statistic. The proposed test statistic makes use of the effect of multipath in code pseudoranges and it does not use time differences. Therefore the detection ability for multipath is improved in most environments. However, the increased number of differences makes the measurement noises larger. The performance of the method is compared with that of the conventional parity space method with code pseudorange.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.57 no.7
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• pp.1247-1253
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• 2008

A design method of a T-S fuzzy model based reduced order nonlinear unknown input observer(NUIO) is presented. The fuzzy NUIO is designed based on the parallel distributed compensation(PDC) concept. It consists of a number of the linear UIOs, each of which is designed for each local linear model in the T-S fuzzy model of a class of nonlinear systems. The fuzzy NUIO provides not only the state estimates insensitive to the unknown inputs, for example, disturbances and faults etc., but also the estimates of the unknown inputs. Therefore, It can be employed in the state feedback control and disturbance rejection control of a class of nonlinear systems with unknown disturbances. It also applied to the robust residual generation for the fault detection and isolation systems and to the design of fault tolerant control systems. As an example, the NUIO is applied to an inverted pendulum system to show the state and disturbance estimation performance and to illustrate the fuzzy reduced order NUIO design method.