Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
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A Study on the Somatic Cells in Quarter Milk Samples of Holstein Cows with Suspected Mastitis

유방염으로 의심된 유우 분방유즙의 체세포 연구

  • Jeong Ji-young (Chonnam Livestock Research Institute) ;
  • Lee Jeong-chi (Department of veterinary nurse and pets sciences, Seojeong College Yangju) ;
  • Lee Chung-gil (College of Veterinary Medicine, Chonnam National University) ;
  • Kim Hye-ra (Chonnam Livestock Research Institute) ;
  • Choi Jong-sung (Chonnam Livestock Research Institute) ;
  • Lee Chai-yong (College of Veterinary Medicine, Chonnam National University)
  • 정지영 (전라남도 축산기술연구소) ;
  • 이정치 (서정대학 애완동물과) ;
  • 이정길 (전남대학교 수의과대학) ;
  • 김혜라 (전라남도 축산기술연구소) ;
  • 최종성 (전라남도 축산기술연구소) ;
  • 이채용 (전남대학교 수의과대학)
  • Published : 2005.09.01
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Abstract

This study was conducted to survey the relationship between the somatic cell count (SCC), and California mastitis test (CMT) & mastitis. A total of 328 quarter milk samples were collected from 211 cows suspected to have mastitis; Both SCC and CMT were performed on the samples. Milk smear was stained with Broadhurst and Paley stain and the cells were classified into either epithelial or blood cells. Bacterial isolation was made and antimicrobial susceptibility was tested. Of the 328 quarters, 78 ($23.8{\%}$) were CMT negative with SCC <750,000/ml. As expected, CMT score increased with the increase of SCC. The number of epithelial cells decreased with the increasing number of somatic cells, while the opposite was the case with the number of blood cells. The critical point was when the SCC reached 1,000,000/ml. Up to 1,000,000 cells/ml, the number of epithelial cells was greater than that of blood cells. The results indicate that when epithelia:blood cell ratio is 58.1:41.9, the milking line should be checked and bacterial isolation be performed on the samples in order to identity mastitis.

Keywords

References

  1. Adkinson RW, Ingawa KH, Blouin DC, Nickerson SC. Distribution of clinical mastitis among quarters of bovine udder. J Dairy Sci, 1993; 76: 3453-3459 https://doi.org/10.3168/jds.S0022-0302(93)77684-5
  2. Bauer AW, Kirby WM, Sherris TC, Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol, 1966; 45: 493-496
  3. Baxter JD, Rogers GW, Spencer SB, Eberhart RJ. The effect of milking machine liner slip on new intramammary infections. J Dairy Sci, 1992; 75: 1015-1018 https://doi.org/10.3168/jds.S0022-0302(92)77844-8
  4. Bramley AJ, Dood FH. Reviews of the progress of dairy science: mastitis control- progress and prospects. J Dairy Res, 1984; 51: 481-512
  5. Broadhurst J, Paley C. A single-dip stain for the direct examination of milk. J Am Vet Med Assoc, 1939; 94: 525-527
  6. Capuco AV, Mein GA, Nickerson SC, Jack LJW, Wood DL, Bright SA, Aschenbrenner RA, Miller RH, Bitrnan J. Influence of pulsationless milking on teat canal keratin and mastitis. J Dairy Sci, 1994; 77: 64-74 https://doi.org/10.3168/jds.S0022-0302(94)76929-0
  7. Chrystal MA, Seykora AJ, Hansen LB. Heritabilities of teat end shape. and teat diameter and their relationships with somatic cell score. J Dairy Sci, 1999; 82: 2017-2022 https://doi.org/10.3168/jds.S0022-0302(99)75439-1
  8. Grindal RJ, Hillerton JE. Influence of milk flow rate on new intramammary infection in dairy cows. J Dairy Res, 1991; 58: 263-268 https://doi.org/10.1017/S0022029900029848
  9. Harmon RJ. Mastitis and genetic evaluation for somatic cell count: physiology of mastitis and factors affecting somatic cell counts. J Dairy Sci, 1994; 77: 2103-2112 https://doi.org/10.3168/jds.S0022-0302(94)77153-8
  10. Jain NC. Common mammary pathogens and factors in infection and mastitis. J Dairy Sci, 1979; 62: 128-134 https://doi.org/10.3168/jds.S0022-0302(79)83214-2
  11. Kehrli Jr ME, Shuster, DE. Factors affecting milk somatic cells and their role in health of the bovine mammary gland. J Dairy Sci, 1979; 77: 619-627 https://doi.org/10.3168/jds.S0022-0302(94)76992-7
  12. Kelly AL, Tiernan D, O'Sullivan C, Joyce P. Correlation between bovine milk somatic cell count and polymorphonuclear leukocyte level for samples of bulk milk and milk from individual cows. J Dairy Sci 2000; 83: 300-304 https://doi.org/10.3168/jds.S0022-0302(00)74878-8
  13. Leitner G, Shoshani E, Krifucks O, Chaffer M, Saran A. Milk leucocyte population patterns in bovine udder infection of different aetiology. J Vet Med B Infect Dis Vet Public Health, 2000; 47: 581-589 https://doi.org/10.1046/j.1439-0450.2000.00388.x
  14. MacFaddin JF. Biochemical tests for identification of medical bacteria. 2nd., Waverly Press, 1980: 166-342
  15. Rasmuseen MD, Madsen NP. Effects of milkline vacuum, pulsator airline vacuum, and cluster weight on milk yield, teat condition, and udder health. J Dairy Sci, 2000; 83: 77-84 https://doi.org/10.3168/jds.S0022-0302(00)74858-2
  16. Rivas AL, Quimby FW, Blue J, Coksaygan O. Longitudinal evaluation of bovine mammary gland health status by somatic cell counting, flow cytometry, and cytology. J Vet Diagn Invest, 2001; 13: 399-407 https://doi.org/10.1177/104063870101300506
  17. Rogers G W, Hargrove GL. Absence of quadratic relationship between genetic evaluation for somatic cell scores and udder linear traits. J Dairy Sci, 1993; 76: 3601-3606 https://doi.org/10.3168/jds.S0022-0302(93)77700-0
  18. Rogers GW, Spencer SB. Relationships among udder and teat morphology and milking characteristics. J Dairy Sci, 1991; 74: 4189-4194 https://doi.org/10.3168/jds.S0022-0302(91)78614-1
  19. Ruban Yu, Vard A. Selection of cows for resistance to mastitis. Molochnoe i Myasnoe Skotovodstvo, 1991; 5: 33-34
  20. Schalm OW, Caroll EJ, Jain NC. Bovine mastitis. Lea and Fiebiger, Philadelphia 1971: 316-329
  21. Seykora AJ, McDaniel BT. Udder and teat morphology related to mastitis resistance: a review. J Dairy Sci, 1985; 68: 2087-2093 https://doi.org/10.3168/jds.S0022-0302(85)81072-9
  22. Seykorya AJ, McDaniel BT. Genetics statistics and relationships of teat and udder traits, somatic cell counts and milk production. J Dairy Sci, 1986; 69: 2395-2407 https://doi.org/10.3168/jds.S0022-0302(86)80679-8
  23. Shook GE. Selection for disease resistance. J Dairy Res, 1989; 72: 1349-1362 https://doi.org/10.3168/jds.S0022-0302(89)79242-0
  24. Slettbakk T, Jorstad A, Farver TB, Holmes JC. Impact of milking characteristics and morphology of udder and teats on clinical mastitis in first- and second-lactation Norwegian cattle. Prev Vet Med, 1995; 24: 235-244 https://doi.org/10.1016/0167-5877(95)00490-N
  25. 문진산, 주이석, 구복경, 장금찬, 김종염, 임흥식, 김병기, 박용호. 체세포 수 등외목장의 착유기 설치 및 운용상의 문제점에 대한 연구. 한국수의공중보건학회지, 1996; 20: 121-126
  26. 박용호. 우군 및 집합유 관리를 통한 체세포 수 감소 방안. 한국유질유방염연구회, 1995: 11-37